Integrated approach of whole-genome analysis, toxicological evaluation and life cycle assessment for pyrene biodegradation by a psychrophilic strain, Shewanella sp. ISTPL2.

Polycyclic aromatic hydrocarbons (PAHs) such as pyrene are universal contaminants existing in the environment which have known cancer-causing and mutagenic characteristics. A psychrophilic bacterial strain Shewanella sp. ISTPL2 was isolated from the sediment sample collected from the Pangong lake, Jammu & Kashmir, India. In our previous study, the pyrene degradation potential of the ISTPL2 strain was studied in both mineral salt media as well as in soil artificially spiked with different concentrations of pyrene. Whole-genome sequencing of ISTPL2 strain in the current study highlighted the key genes of pyrene metabolism, including alcohol dehydrogenase and ring hydroxylating dioxygenase alpha-subunit. Pyrene cytotoxicity was evaluated on HepG2, a human hepato-carcinoma cell line. The cytotoxicity of the organic extract decreased with the increasing duration of bacterial treatment. To develop a more sustainable biodegradation approach, the potential impacts were evaluated for human health and ecosystem using life-cycle assessment (LCA) following the ReCiPe methodology for the considered PAH. The results implemented that global warming potential (GWP) had the highest impact, whereas both ecotoxicity and human toxicity had least from this study.

Environmental DNA and RNA as Records of Human Exposome, Including Biotic/Abiotic Exposures and Its Implications in the Assessment of the Role of Environment in Chronic Diseases.

Most of environment-related diseases often result from multiple exposures of abiotic and/or biotic stressors across various life stages. The application of environmental DNA/RNA (eDNA/eRNA) to advance ecological understanding has been very successfully used. However, the eminent extension of eDNA/eRNA-based approaches to estimate human exposure to biotic and/or abiotic environmental stressors to understand the environmental causes of chronic diseases has yet to start. Here, we introduce the potential of eDNA/eRNA for bio-monitoring of human exposome and health effects in the real environmental or occupational settings. This review is the first of its kind to discuss how eDNA/eRNA-based approaches can be applied for assessing the human exposome. eDNA-based exposome assessment is expected to rely on our ability to capture the genome- and epigenome-wide signatures left behind by individuals in the indoor and outdoor physical spaces through shedding, excreting, etc. Records of eDNA/eRNA exposome may reflect the early appearance, persistence, and presence of biotic and/or abiotic-exposure-mediated modifications in these nucleic acid molecules. Functional genome- and epigenome-wide mapping of eDNA offer great promise to help elucidate the human exposome. Assessment of longitudinal exposure to physical, biological, and chemical agents present in the environment through eDNA/eRNA may enable the building of an integrative causal dynamic stochastic model to estimate environmental causes of human health deficits. This model is expected to incorporate key biological pathways and gene networks linking individuals, their geographic locations, and random multi-hits of environmental factors. Development and validation of monitoring of eDNA/eRNA exposome should seriously be considered to introduce into safety and risk assessment and as surrogates of chronic exposure to environmental stressors. Here we highlight that eDNA/eRNA reflecting longitudinal exposure of both biotic and abiotic environmental stressors may serve as records of human exposome and discuss its application as molecular tools for understanding the toxicogenomics basis of environment-related health deficits.

Assessment of methane emissions and energy recovery potential from the municipal solid waste landfills of Delhi, India.

Rising rate of MSW generation and unscientific disposal in the open dumping sites are responsible for emission of high concentrations of methane in developing countries. IPCC Default method (DM), First-order decay (FOD) and LandGEM were used to estimate methane emissions from the unengineered landfill sites of Delhi-Okhla, Bhalswa and Ghazipur between 1984 and 2015. During the period, the total CH4 emissions was found to be 1288.99, 311.18, 779.32 Gg from the 3 landfill sites of Delhi as predicted by DM, FOD and LandGEM respectively. The energy generation potential from methane for the year 2015 was found to vary from 4.16 × 108 to 9.86 × 108 MJ for Ghazipur, 2.08 × 108 to 4.06 × 108 MJ for Okhla and 3.42 × 108 to 8.11 × 108 MJ for Bhalswa. Efficient utilization of methane from the landfills as an energy source can be a sustainable waste management option.

Characterization of leaf waste based biochar for cost effective hydrogen sulphide removal from biogas.

Installation of decentralized units for biogas production along with indigenous upgradation systems can be an effective approach to meet growing energy demands of the rural population. Therefore, readily available leaf waste was used to prepare biochar at different temperatures and employed for H2S removal from biogas produced via anaerobic digestion plant. It is found that biochar prepared via carbonization of leaf waste at 400 °C effectively removes 84.2% H2S (from 1254 ppm to 201 ppm) from raw biogas for 25 min in a continuous adsorption tower. Subsequently, leaf waste biochar compositional, textural and morphological properties before and after H2S adsorption have been analyzed using proximate analysis, CHNS, BET surface area, FTIR, XRD, and SEM-EDX. It is found that BET surface area, pore size, and textural properties of leaf waste biochar plays a crucial role in H2S removal from the biogas.

An integrated approach to study the risk from landfill soil of Delhi: Chemical analyses, in vitro assays and human risk assessment.

In the present study, landfill soil of three municipal solid waste landfill sites of Delhi, India were toxico-chemically analyzed for human risk assessment as inadequate information is available on the possible health effects of the contaminants present in landfill soil. The landfill soil samples were prepared for analyzing heavy metal concentration, organic contaminants and toxicity analysis separately. Composite soil sample collected from three landfill sites were analyzed for heavy metal by ICP-AES. Metal concentration so obtained was below the permissible limit of soil but higher than the set limits for effluent. Some of the persistent organic contaminants like phthalates, benzene derivatives, halogenated aliphatic compounds and PAHs derivatives were detected by scan mode GC-MS. Further, concentration of 17 polycyclic aromatic hydrocarbons (PAHs) in landfill soil of Delhi was evaluated by selective ion monitoring GC-MS in order to ascertain their contamination levels and potential health risk. The concentration of total PAHs in the samples ranged from 192 to 348µg/kg. The maximum concentrations of PAHs were found in Ghazipur landfill site followed by Okhla and Bhalswa landfills. Cancer risk (CR) values of sampling sites were within the acceptable range for adults, adolescents and children (both male and female) suggesting that PAHs present in landfill soil are unlikely to pose any cancer risk for population based on dermal contact, ingestion and inhalation exposure pathways. However, landfill soil organic extract showed significant cytotoxic and genotoxic effects on HepG2 cell line as revealed by MTT and Comet assays respectively. The observed MTT EC50 values ranged from 7.58 to 12.9g SedEq/Lalong with statistically significant DNA damage. Thus, although the soil organic extract contained low concentrations of PAHs with negligible carcinogenic potential, but the mixture of organic pollutants present in soil were found to be toxic enough to affect human health due to their synergistic or additive actions.

Bioassays for toxicological risk assessment of landfill leachate: A review.

Landfilling is the most common solid waste management practice. However, there exist a potential environmental risk to the surface and ground waters due to the possible leaching of contaminants from the landfill leachates. Current municipal solid waste landfill regulatory approaches consider physicochemical characterization of the leachate and do not assess their potential toxicity. However, assessment of toxic effects of the leachates using rapid, sensitive and cost-effective biological assays is more useful in assessing the risks as they measure the overall toxicity of the chemicals in the leachate. Nevertheless, more research is needed to develop an appropriate matrix of bioassays based on their sensitivity to various toxicants in order to evaluate leachate toxicity. There is a need for a multispecies approach using organisms representing different trophic levels so as to understand the potential impacts of leachate on different trophic organisms. The article reviews different bioassays available for assessing the hazard posed by landfill leachates. From the review it appears that there is a need for a multispecies approach to evaluate leachate toxicity.

Emerging role of Geographical Information System (GIS), Life Cycle Assessment (LCA) and spatial LCA (GIS-LCA) in sustainable bioenergy planning.

Sustainability of a bioenergy project depends on precise assessment of biomass resource, planning of cost-effective logistics and evaluation of possible environmental implications. In this context, this paper reviews the role and applications of geo-spatial tool such as Geographical Information System (GIS) for precise agro-residue resource assessment, biomass logistic and power plant design. Further, application of Life Cycle Assessment (LCA) in understanding the potential impact of agro-residue bioenergy generation on different ecosystem services has also been reviewed and limitations associated with LCA variability and uncertainty were discussed. Usefulness of integration of GIS into LCA (i.e. spatial LCA) to overcome the limitations of conventional LCA and to produce a holistic evaluation of the environmental benefits and concerns of bioenergy is also reviewed. Application of GIS, LCA and spatial LCA can help alleviate the challenges faced by ambitious bioenergy projects by addressing both economics and environmental goals.

Biosorption of landfill leachate by Phanerochaete sp. ISTL01: isotherms, kinetics and toxicological assessment.

The study investigates the ability of fungus Phanerochaete sp. ISTL01 for biosorption of color from landfill leachate. Batch mode experiments were conducted to study the effects of pH, temperature, adsorbent dose, contact time and initial leachate concentration on biosorption. Maximum biosorption capacity was determined as 17.73 mg g-1 of biomass. Equilibrium isotherms and kinetics were further studied. The biosorption data were found to fit well to the Freundlich isotherm and pseudo-second-order kinetic model. The value of activation energy suggested that chemisorption mechanism was involved. Biosorption efficiency was also evaluated by the Methyltetrazolium (MTT) assay for cytotoxicity and alkaline comet assay in HepG2 human hepato-carcinoma cells. The fungus reduced toxicity as shown by 1.3-fold increase in MTT EC50 and 1.5- and 1.1-fold reduction in Tail moment and Olive tail moment, respectively, after 12 h biosorption. The fungus showed good biosorption characteristics in terms of contaminant-level reduction per unit mass of adsorbent, process kinetics and toxicity reduction, envisaging its application in leachate treatment.

Landfill leachate treatment using bacto-algal co-culture: An integrated approach using chemical analyses and toxicological assessment.

The present study aims to evaluate the feasibility of leachate treatment using a synergistic approach by microalgae and bacteria. Leachate from one of the landfill of Northern India showed the presence of various toxic organic contaminants like naphthalene, benzene, phenol and their derivatives, napthols, pesticides, epoxides, phthalates and halogenated organic compounds. ICP-AES analysis revealed high concentrations of Zn, Cr, Fe, Ni, and Pb beyond the maximum permissible limit of discharge. Bacto-algal co-culture was found to be the most efficient in removal of toxic organic contaminants and heavy metals. Further, detoxification efficiency of bacto-algal treatment was evaluated by Methyl tetrazolium (MTT) assay for cytotoxicity and alkaline comet assay for genotoxicity using hepatoma HepG2 cells. Reduction in toxicity was confirmed by an increase in LC50 by 1.9 fold and reduction in Olive Tail Moment by 40.6 fold after 10 days of treatment. Results of the study indicate bioremediation and detoxification potency of bacto-algal co-culture for leachate treatment.

Intake estimates of phthalate esters for South Delhi population based on exposure media assessment.

An indirect estimation method was followed to derive exposure levels of fifteen phthalate congeners in urban population of Delhi, India. The exposure media samples were collected from Jawaharlal Nehru University (JNU) campus and Okhla industrial area. GC-MS analysis of the samples indicated di(2-ethylhexyl) phthalate (DEHP) to be the most abundant congener and its estimated total daily intake level reached upto 70 µg kg(-1) d(-1). Out of the studied congeners, intake doses for di-n-butyl phthalate (DnBP) and DEHP, reached levels near or above the established exposure limit. In JNU, DEHP, dimethyl phthalate (DMP) and butyl benzyl phthalate (BBP) had 69% share in combined daily intake of Σ15 phthalates (CDI15); whereas, in Okhla, DEHP, diethyl phthalate (DEP), diisobutyl phthalate (DIBP), DnBP and DMP shared 64% of the CDI15. Food was found to be the major source of exposure contributing 67% and 74% of the estimated CDI15 at JNU and Okhla respectively.

Assessment of in vitro cyto/genotoxicity of sequentially treated electroplating effluent on the human hepatocarcinoma HuH-7 cell line.

The present study compares in vitro toxicity of electroplating effluent after the batch treatment process with that obtained after the sequential treatment process. Activated charcoal prepared from sugarcane bagasse through chemical carbonization, and tolerant indigenous bacteria, Bacillus sp. strain IST105, were used individually and sequentially for the treatment of electroplating effluent. The sequential treatment involving activated charcoal followed by bacterial treatment removed 99% of Cr(VI) compared with the batch processes, which removed 40% (charcoal) and 75% (bacteria), respectively. Post-treatment in vitro cyto/genotoxicity was evaluated by the MTT test and the comet assay in human HuH-7 hepatocarcinoma cells. The sequentially treated sample showed an increase in LC50 value with a 6-fold decrease in comet-assay DNA migration compared with that of untreated samples. A significant decrease in DNA migration and an increase in LC50 value of treated effluent proved the higher effectiveness of the sequential treatment process over the individual batch processes.

Assessment of Cryptococcus albidus for biopulping of eucalyptus.

Cryptococcus albidus shows delignification activity in nature. It was used for the biopulping of eucalyptus wood (Eucalyptus grandis) to access its potential for industrial application in the pulp and paper industry. Enzyme analysis on days 15, 30, and 60 showed the presence of laccase and xylanase as key enzymes. The production of endo-glucanase (CMCase) and exo-glucanase (FPase) was very low. Scanning electron microscopy (SEM) showed the surface colonization of wood and loosening of wood fibers in C. albidus-treated samples. Fourier-transformation infrared spectroscopy (FT-IR) indicated the chemical modification of eucalyptus wood. Denaturing gradient gel electrophoresis (DGGE) analysis on days 15, 30, and 60 confirmed the presence of C. albidus throughout the experiments. Cryptococcu albidus was able to suppress the growth of a native population. Further, after 60 days both the control and treated eucalyptus wood chips were given kraft pulping treatment. The kappa number of pulp of control wood was 21 and for treated wood was 17. Kappa number is considered a measure of lignin content in wood; hence the treatment of eucalyptus by C. albidus (biopulping) was effective in reducing its lignin content and can be used for biopulping in the pulp and paper industry.