Nutritional inequalities structure worker division of labor in social insects.

Eusocial insect societies are fundamentally non-egalitarian. The reproductive caste 'wins' in terms of resource accumulation, whereas non-reproductive workers 'lose'. Here, we argue that the division of labor among workers is also organized by nutritional inequalities. Across vastly different social systems and a variety of hymenopteran species, there is a recurrent pattern of lean foragers and corpulent nest workers. Experimental manipulations confirm causal associations between nutritional differences, associated molecular pathways, and behavioral roles in insect societies. The comparative and functional genomic data suggest that a conserved toolkit of core metabolic, nutrient storage, and signaling genes has evolved to regulate the social insect division of labor. Thus, the unequal distribution of food resources can be considered a fundamental organizing factor in the social insect division of labor.

A survey of DNA methylation across social insect species, life stages, and castes reveals abundant and caste-associated methylation in a primitively social wasp.

DNA methylation plays an important role in the epigenetic control of developmental and behavioral plasticity, with connections to the generation of striking phenotypic differences between castes (larger, reproductive queens and smaller, non-reproductive workers) in honeybees and ants. Here, we provide the first comparative investigation of caste- and life stage-associated DNA methylation in several species of bees and vespid wasps displaying different levels of social organization. Our results reveal moderate levels of DNA methylation in most bees and wasps, with no clear relationship to the level of sociality. Strikingly, primitively social Polistes dominula paper wasps show unusually high overall DNA methylation and caste-related differences in site-specific methylation. These results suggest DNA methylation may play a role in the regulation of behavioral and physiological differences in primitively social species with more flexible caste differences.