Enzymatic probe sonication for quick extraction of total bisphenols from animal-derived foods: Applicability to occurrence and exposure assessment.

A high demand exists in bisphenols (BPs) screening studies for quick, reliable and straightforward analytical methods that generate data faster and simultaneously. Herein, we describe a combination of enzymatic probe sonication (EPS) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for quick extraction and simultaneous quantification of eight important BPs in animal-derived foods. Results obtained demonstrated that the ultrasonic probe power could not only enhance the enzymatic hydrolysis efficiency, but also accelerate the liquid-liquid extraction procedure. Under optimized EPS parameters, one sample could be exhaustively extracted within 120 s, as compared with 12 h needed for the conventional enzymatic extraction which is more suitable for high-throughput analysis. The method was successfully applied to analyze residual BPs in animal-derived foods collected from Beijing, China. Widespread occurrence of BPA, BPS, BPF, BPAF, BPP, and BPB were found, with detection frequencies of 65.2%, 42.4%, 33.7%, 29.4%, 28.3%, and 27.2%, respectively. The highest total concentration levels of BPs (sum of the eight BPs analyzed, ΣBPs) were found in chicken liver (mean 12.2 µg/kg), followed by swine liver (6.37 µg/kg), bovine muscle (3.24 µg/kg), egg (2.03 µg/kg), sheep muscle (2.03 µg/kg), chicken muscle (1.45 µg/kg), swine muscle (1.42 µg/kg), and milk (1.17 µg/kg). The estimated daily intake (EDI) of BPs, based on the mean and 95th percentile concentrations and daily food consumptions, was estimated to be 5.687 ng/kg bw/d and 22.71 ng/kg bw/d, respectively. The human health risk assessment in this work suggests that currently BPs do not pose significant risks to the consumers because the hazard index (HI) was <1.

The occurrence of perfluoroalkyl acids in an important feed material (fishmeal) and its potential risk through the farm-to-fork pathway to humans.

Few studies have attempted to elucidate the occurrence of perfluoroalkyl acids (PFAAs) in animal feeds and how they play a role in human ingestion. Fishmeal was the most important animal-derived feed in global husbandry and may have been subjected to PFAA contamination considering the PFAAs' ubiquitous distribution in aquatic ecosystems. We collected ninety-two commercial fishmeal from the most important fishmeal-producing countries and found that Σ16PFAAs ranged from 0.65 to 85.5 ng/g (average: 18.2 ng/g, 12% moisture). PFOS still predominated, with unexpected high detection of PFUnDA. The wide occurrence of short-chain PFAAs (e.g., PFBA, PFBS) in fishmeal were found for the first time. From a geographical view, PFAA levels in fishmeal that originated from the Northern Hemisphere were significantly higher than those from the Southern Hemisphere (p < 0.01). Higher levels of under-studied long-chain PFAAs (PFNA, PFDA, PFUnDA, PFDoDA and PFTrDA) weighted more in industrialized areas than less industrialized areas, whereas the legacy PFAAs (PFOS, PFOA and PFHxS) were comparable among all regions. The estimated daily intake was calculated from animal feed to humans (via cultured fish) from 20 Chinese provinces by the Monte Carlo Simulation. A proportion of 29.8% of residents from the Fujian Province exceeded the EFSA's suggestion for PFOS ingestion.