Performance assessment of DNA sequencing platforms in the ABRF Next-Generation Sequencing Study.

Assessing the reproducibility, accuracy and utility of massively parallel DNA sequencing platforms remains an ongoing challenge. Here the Association of Biomolecular Resource Facilities (ABRF) Next-Generation Sequencing Study benchmarks the performance of a set of sequencing instruments (HiSeq/NovaSeq/paired-end 2 × 250-bp chemistry, Ion S5/Proton, PacBio circular consensus sequencing (CCS), Oxford Nanopore Technologies PromethION/MinION, BGISEQ-500/MGISEQ-2000 and GS111) on human and bacterial reference DNA samples. Among short-read instruments, HiSeq 4000 and X10 provided the most consistent, highest genome coverage, while BGI/MGISEQ provided the lowest sequencing error rates. The long-read instrument PacBio CCS had the highest reference-based mapping rate and lowest non-mapping rate. The two long-read platforms PacBio CCS and PromethION/MinION showed the best sequence mapping in repeat-rich areas and across homopolymers. NovaSeq 6000 using 2 × 250-bp read chemistry was the most robust instrument for capturing known insertion/deletion events. This study serves as a benchmark for current genomics technologies, as well as a resource to inform experimental design and next-generation sequencing variant calling.

Assessment of Additive Manufacturing for Increasing Sustainability and Productivity of Smallholder Agriculture.

The System of Rice Intensification (SRI) seeks to increase both the sustainability and the productivity of smallholder rice farms, but the adoption of this methodology is constrained by local access to appropriate mechanical equipment. Valuable information about real difficulties in the adoption of SRI was collected through communications with five field partners. Two primary mechanization obstacles were identified, that is, the availability and performance of a roller component on a push seeder and a rotor component on a push weeder. The potential of additive manufacturing (AM), and especially material extrusion three-dimensional (3D) printing (ME3DP), to assist in overcoming the identified obstacles was assessed for two cases while considering both local prototyping and low-volume production. A simplified cost model was used to compare with the cost of manufacturing both in the United States and locally in the field. The acquired data suggests that in specific cases current ME3DP technology can more rapidly provide functional parts, accelerating the design cycle and lowering cost by about a factor of 10 relative to local fabrication routes. In the case where mechanical performance is critical and dimensional precision and surface finish are not, wire arc metal AM appears promising, but it is not as economical as fabrication by traditional means in the field.