Simultaneous detection of IgE binding to several allergens using a nitrocellulose 'polydisc'.

The use of nitrocellulose for allergen disc preparation permits several different allergens to be applied as separate spots to the same disc for simultaneous evaluation with one serum sample (50 microliter). To achieve this, an immunological application method involving anti-human IgE and the peroxidase-anti-peroxidase procedure must be used with a substrate that produces an insoluble product. The suitability of the polydiscs for routine clinical evaluation of several allergens at once was demonstrated by testing the sera of seven allergic bakers with discs containing extracts of flour or pollen from wheat, cereal rye, or rye grass.