Asterias Rolleston starfish gonad lipids: A novel source of Omega-3 fatty acids - assessment of major components and their antioxidant activities.

The major lipids and antioxidant activities of Asterias rolleston gonad lipids were evaluated systematically. Major lipids of A. Rolleston gonad lipids were triacylglycerols (TAGs) and phospholipids (PLs). Total lipids were composed of 15.62% of polyunsaturated fatty acids (PUFAs), and 40.81% of monounsaturated fatty acids (MUFAs). The most abundant PUFA were C20:5n-3 (EPA) (6.28%) and C22:6n-3 (DHA) (5.80%). Predominantly composed of phosphatidylcholine (PC) and phosphatidylethanolamine (PE), polar lipids were rich in PUFAs and could contain up to 34.59% EPA and DHA, and PE and PI (phosphatidylinositol) were also found to be the main carriers of EPA and ARA (arachidonic acid) in polar lipids. The MUFA and PUFA of Sn-2 in TAG are 39.72% and 30.37%, respectively. A total of 64 TAG species were identified, with Eo-P-M, Eo-Eo-M, and M-M-Eo being the main TAGs components. Moreover, A. rollestoni gonad lipids exhibited potent radical scavenging activities and reducing power in a dose-dependent manner.

Varietal Authenticity Assessment of QTMJ Tea Using Non-Targeted Metabolomics and Multi-Elemental Analysis with Chemometrics.

In this paper, a combination of non-targeted metabolomics and multi-element analysis was used to investigate the impact of five different cultivars on the sensory quality of QTMJ tea and identify candidate markers for varietal authenticity assessment. With chemometric analysis, a total of 54 differential metabolites were screened, with the abundances significantly varied in the tea cultivars. By contrast, the QTMJ tea from the Yaoshan Xiulv (XL) monovariety presents a much better sensory quality as result of the relatively more abundant anthocyanin glycosides and the lower levels of 2'-o-methyladenosine, denudatine, kynurenic acid and L-pipecolic acid. In addition, multi-elemental analysis found 14 significantly differential elements among the cultivars (VIP > 1 and p < 0.05). The differences and correlations of metabolites and elemental signatures of QTMJ tea between five cultivars were discussed using a Pearson correlation analysis. Element characteristics can be used as the best discriminant index for different cultivars of QTMJT, with a predictive accuracy of 100%.

The impact of digital economy on the export competitiveness of China's manufacturing industry.

The digital economy is an important driving force for the high-quality development of the manufacturing industry. This paper uses 2013-2019 manufacturing panel data to empirically test the impact of digital economic growth on China's manufacturing export competitiveness. The results show that, first, the degree of integration of manufacturing and digital economy in the eastern region is higher than that in the central and western regions. Second, the development of the digital economy has indeed had a significant positive impact on the export competitiveness of China's manufacturing industry. Third, the digital economy has different impacts on the export competitiveness of manufacturing industries in different industries and regions. Based on the findings, some suggestions are made to further enhance the export competitiveness of China's manufacturing industry, such as paying attention to the development of digital infrastructure, optimizing the mode of integration of the manufacturing sector and the digital economy, and enhancing international collaboration in digital technology innovation.

Participating in health insurance and health improvements for the relatively poor population: A propensity score analysis.

This study examined the causal relationship between participation in the New Rural Cooperative Medical Scheme (NRCMS) and health status among relatively poor population in rural China. Data were obtained from the China Family Panel Studies (CFPS) conducted in 2018, which contained 4,507 samples. This study used propensity score matching (PSM) to examine the net effect of participation in the NRCMS on the health of the relatively poor population, and this effect was tested for equilibrium using nearest neighbor matching, radius matching, and kernel matching. This study showed that participation in the NRCMS has a significant and positive effect on the health status of the relatively poor population and the positive health effect may come from three channels, including the increased frequency of physical activity, the fact that an individual is more likely to seek medical care at a lower level of visit, and a plan to reduce health care expenditures.

Factors Influencing Public Participation in Community Disaster Mitigation Activities: A Comparison of Model and Nonmodel Disaster Mitigation Communities.

Public participation in community-organized disaster mitigation activities is important for improving disaster mitigation capacity. With data from 260 questionnaires, this study compared the current status of public participation in model disaster mitigation communities and nonmodel communities in a geological-disaster-prone area. Three community-organized disaster mitigation education activities were compared cross-sectionally. A binary logistic regression was used to analyze the effects of attitude, perceived behavioral control, disaster experience, and other key factors on the public's choice to participate in community disaster mitigation activities. The analysis results indicated that model communities had higher public participation in two efforts, evacuation drills and self-help skills training, and lower participation in activities that invited them to express their feedback than nonmodel communities. The influence of attitudinal factors on the decision to participate in disaster mitigation activities had a high similarity across community types. The public participation in model disaster mitigation communities is influenced by factors such as subjective norms and participation cognition; the behavior of people in nonmodel communities is influenced by factors such as previous experience with disasters, perceived behavioral control, risk perception, and participation cognition and has a greater potential for disaster mitigation community construction. This study provides practical evidence and theoretical support for strengthening the sustainable development of disaster mitigation community building.

Combination of Endogenous Estradiol and Adipokine Leptin in Breast Cancer Risk and Prognosis Assessment in Postmenopausal Chinese Women.

Objective: Our study aims to clarify the role of estradiol and leptin in breast cancer risk and prognostic assessment in postmenopausal Chinese women. Design: The serum circulating estradiol and leptin level was detected by ELISA. Then the correlation between estradiol, leptin level, and clinical characteristics was analyzed using Fisher's exact test. Next, the Kaplan-Meier model was used to analyze the association between estradiol, leptin, and prognosis of postmenopausal breast cancer patients in our cohort and the TCGA dataset. Setting: The study was conducted at the National Cancer Center, Cancer Hospital, Chinese Academy of Medical Sciences, and Peking Union Medical College. Patients: A total of 182 postmenopausal breast cancer patients and 111 healthy subjects from January 2010 to August 2010 were included in the analysis. Another 702 cases with breast cancer were retrieved from The Cancer Genome Atlas (TCGA) database for subsequent analysis. Main Outcome Measure: Serum circulating estradiol and leptin level. Results: The level of estradiol was significantly higher (P<0.001) but the level of leptin had no significant difference (P = 0.764) in postmenopausal breast cancer patients compared with healthy subjects. The level of estradiol and leptin was not significantly different between estrogen receptor (ER) positive and ER-negative groups (P>0.05). Estradiol was significantly correlated with tumor T stage (P = 0.002) and leptin was significantly associated with perineural invasion (P = 0.014). In addition, the disease-free survival of patients with a high level of estradiol was significantly shorter (P = 0.025) but leptin tended to be a protective factor for overall survival in TCGA analysis (P = 0.038). Conclusion: Circulating estradiol and leptin played important roles in the risk of postmenopausal breast cancer even in low-estrogen nations with an independent expression of ER status. High circulating estradiol was a poor prognostic factor and leptin may be a protection signal in Chinese postmenopausal patients with breast cancer.

Assessment of antimicrobial and wound healing effects of Brevinin-2Ta against the bacterium Klebsiella pneumoniae in dermally-wounded rats.

Antimicrobial peptides (AMPs) are regarded as promising alternatives for antibiotics due to their inherent capacity to prevent microbial drug resistance. Amphibians are rich source of bioactive molecules, which provide numerous AMPs with various structures as drug candidates. Here, we isolated and identified a novel AMP Brevinin-2Ta (B-2Ta) from the skin secretion of the European frog, Pelophylax kl. esculentus. In vitro studies revealed that it showed broad antimicrobial activities against S. aureus, E. coli and C. albicans with low cytotoxicity to erythrocytes. Furthermore, we examined the anti-inflammation effect in vivo by using Klebsiella pneumoniae-infected Sprague-Dawley (SD) rats. The wound closure outcomes revealed that B-2Ta effectively restrained the bacterial infection at a dose of 10 times minimal inhibitory concentration (MIC) during the 14 days of the wound healing process. Ultra-structure analyses showed that B-2Ta caused structural damage to the microorganism, and bacterial culture found that the number of microbes was significantly reduced by the end of treatment. Immunohistochemistry for the inflammatory marker IL-10 and the endothelial cell marker CD31 suggested positive effects on inflammatory status and epithelial migration and angiogenesis following treatment of the infected granulation tissues with B-2Ta. These results exhibited the continuous phase of inflammation reduction and wound healing acceleration in the B-2Ta-modulated re-epithelialisation of K. pneumoniae infected rats. Taken together, these data demonstrated that B-2Ta has great potential to be developed as antibacterial agents in clinic.