A highly selective optical sensor Eu-BINAM for assessment of high sensitivity cardiac troponin tumor marker in serum of cancer patients.

A novel, easy, touchy and selective spectrofluorimetric technique has been successfully applied for sensitive determination of High Sensitivity Cardiac Troponin (TNHS I) in the serum samples of patients suffering malignant tumors through the usage of optical sensor Eu3+-BINAM complex. The technique is primarily based on quenching of the Eu3+-BINAM complex's luminescence intensity upon introducing various concentrations of High Sensitivity Cardiac Troponin (TNHS I). The synthesis and characterization of the optical sensor was performed via absorption and emission. The sensor was also adapted to offer excitation at 394 nm in acetonitrile at pH 7.5. Concentration of High Sensitivity Cardiac Troponin (TNHS I) in serum samples was found to be proportional to the luminescence intensity quenching of the Eu3+-BINAM complex, most prominently at λem = 618 nm. The limit of the dynamic range is 4.26 × 10-4 to 2 ng/mL. The limit of detection and quantitation were calculated to be 1.35 and 4.10 ng/mL, respectively. The suggested analytical approach proved its applicability, simplicity and comparatively interference- free. The technique was effectively recruited to quantify High Sensitivity Cardiac Troponin (TNHS I) in human serum samples. The proposed technique could be further extended to evaluate some biomarkers associated with malignancy related diseases in human.

An assessment of the role of surgical loupe technique in prevention of postthyroidectomy complications: a comparative prospective study.

The magnification technique offered by surgical loupe is a new method that enhances visualization and helps head and neck surgeons with recurrent laryngeal nerve (RLN) and parathyroid glands identification. This study aimed to assess the safety and efficacy of using binocular surgical loupes in thyroidectomy procedures. Material and Methods: Eighty patients with thyroid nodules who underwent thyroidectomy procedure were divided randomly into two comparable groups, group A subjected to thyroidectomy by using binocular magnification loupe, group B underwent conventional thyroidectomy without using magnification. Patients' demographics, operation time, and postoperative morbidities were recorded. All cases had preoperative and postoperative vocal cords assessment by video laryngoscopy. Pathology, laboratory, and radiology investigations were also conducted. Results: Out of 80 patients, there were 58 females and 22 males. Benign thyroid pathology was found in 74 patients and malignant pathology in 6 patients. The mean operating time was 106 min in group A compared to 138.5 min in group B. The mean amount of intraoperative bleeding was 30 ml in group A while 50 ml in group B. There were no cases of the external branch of the superior laryngeal nerve in both groups; there was better identification in group A. There was only one patient who suffered from a temporary RLN injury in group A, while three cases of temporary and one case of permanent RLN injury were recorded in group B. Permanent hypoparathyroidism was diagnosed in only one patient in group B. Conclusion: The utilization of binocular surgical loupe magnification in thyroid surgery is considered a safe and effective maneuver that has the advantages of decreasing the overall operating time and significantly reducing postoperative complications.

Comparative assessment of frictional forces between differently designed esthetic brackets during simulated canine retraction.

OBJECTIVES: To evaluate force loss due to friction (FR) with an emphasis on esthetic brackets and their design differences during simulated canine retraction. MATERIALS AND METHODS: The tested brackets were round and sharp-cornered conventional-ligating brackets and round-cornered self-ligating brackets. The tested archwires were stainless steel (0.018â€¯× 0.025″ and 0.019â€¯× 0.025″, and 0.018″) archwires. A total of 90 bracket-archwire combinations in 9 equally-sized groups (n = 10) were analyzed. Canine retraction was experimentally simulated in a biomechanical set-up utilizing the custom-made orthodontic measurement and simulation system (OMSS) using a NiTi coil spring that delivered a constant force of 1 N. The simulated retraction path was up to 4 mm. FR was compared among groups using the Welch t­test. Significance level (α) was set to 0.05. RESULTS: The round-cornered conventional-ligating bracket exhibited the least FR (28.6 ± 5.4%), while there were no significant differences in FR between the round-cornered conventional-ligating bracket and the round-cornered self-ligating bracket with 0.018″ stainless steel wires. However, the round-cornered self-ligating bracket exhibited the least FR (34.9 ± 5.1% and 39.3 ± 4.6%) with 0.018â€¯× 0.025″ and 0.019â€¯× 0.025″ stainless steel archwires, respectively. The sharp-cornered conventional-ligating bracket showed the highest FR of 72.4 ± 3.0% among the bracket systems tested in this study. CONCLUSIONS: The round-cornered conventional-ligating bracket showed less FR when compared to sharp-cornered conventional-ligating bracket. Conversely, the round-cornered conventional-ligating bracket exhibited greater FR when compared to the round-cornered self-ligating bracket, with an exception with respect to the 0.018″ wire. In general, FR increased with increased wire dimension.

Metabolite Profiling and Biological Activity Assessment of Casuarina equisetifolia Bark after Incorporating Gold Nanoparticles.

OBJECTIVE: Casuarina equisetifolia bark is rich in various active metabolites and selected to be studied due to limitation of the synthetic antioxidants that have adverse side effects. The present study aimed to enhance efficiency of the most effective extract by incorporating gold nanoparticles (Au-NPs). METHODS: The phytochemical and biological measurements were carried out in total methanolic extract and its successive fractions. Moreover, these measurements were assayed in the most effective extract after incorporating Au-NPs. RESULTS: The study revealed that total methanolic extract exhibited the highest biological and cytotoxic activities as compared to other fractions. Therefore, it is considered as good candidate for nano-extract preparation. The methanolic extract incorporated with Au-NPs showed higher antioxidant, scavenging and cytotoxic activities in addition to higher inhibitory effect against α-amylase activity as compared to native extract itself. To pinpoint active agents in total methanolic extract, the secondary metabolite profiling via HPLC-MS showed that 33 and 17 metabolites were annotated in the extract before and after incorporating Au-NPs, respectively. The median lethal dose (LD50) showed that gold total methanolic nano-extract is safer than total methanolic extract. CONCLUSION: This study concluded that total methanolic C. equisetifolia bark extract is a valuable bioresource to synthesize an eco-friendly Au-NPs with health-enhancing effect as antioxidant, antidiabetic and cytotoxic agents. The present study is considered as the first report on utilization of C. equisetifolia bark in synthesis of Au-NPs by mean of green nanotechnology and investigation of its biological activity in relation to its metabolite fingerprint.

A systematic and robust assessment of hot-melt extrusion-based amorphous solid dispersions: Theoretical prediction to practical implementation.

Amorphous solid dispersions (ASDs) have gained attention as a formulation strategy in recent years, with the potential to improve the apparent solubility and, hence, the oral bioavailability of poorly soluble drugs. The process of formulating ASDs is commonly faced with challenges owing to the intrinsic physical and chemical instability of the initial amorphous form and the long-term physical stability of drug formulations. Numerous research publications on hot-melt extrusion (HME) technology have demonstrated that it is the most efficient approach for manufacturing reasonably stable ASDs. The HME technique has been established as a faster scale-up production strategy for formulation evaluation and has the potential to minimize the time to market. Thermodynamic evaluation and theoretical predictions of drug-polymer solubility and miscibility may assist to reduce the product development cost by HME. This review article highlights robust and established prediction theories and experimental approaches for the selection of polymeric carriers for the development of hot melt extrusion based stable amorphous solid dispersions (ASDs). In addition, this review makes a significant contribution to the literature as a pilot guide for ASD assessment, as well as to confirm the drug-polymer compatibility and physical stability of HME-based formulations.

A highly selective and sensitive spectrofluorimetric method for the assessment of 3-nitrotyrosine in serum using (Eu(TTA)3Phen) photo probe.

A simple, accurate and fast method was developed for the assessment of 3-nitrotyrosine as a biomarker for the early diagnosis of liver cirrhosis with minimal hepatic encephalopathy (MHE) using a (Eu(TTA)3Phen) photo probe. 3-Nitrotyrosine can remarkably quench the luminescence intensity of the (Eu(TTA)3Phen) complex in DMSO at pH = 9 and λ em = 617 nm. The quenching of the luminescence intensity of (Eu(TTA)3Phen) complex particularly the electrical emission band at λ em = 617 nm is used for the assessment of 3-nitrotyrosine in different serum samples of patients with liver cirrhosis.

Assessment of the Biological Activities of Egyptian Purslane (Portulaca oleracea) Extract after Incorporating Metal Nanoparticles, in Vitro and in Vivo Study.

OBJECTIVE: Egyptian Purslane (Portulaca oleracea) is rich in omega-3 fatty acids and a wide range of vitamins and phyto-constituents that were absorbed slowly due to their high molecular weights. Therefore, this study was designed to accelerate the absorption of these phyto-constituents and hence increase their bioavailability by incorporating silver (Ag-NPs) and zinc oxide nanoparticles (ZnO-NPs) due to their impressive properties. METHODS: The major phyto-constituents and different biological activities were quantified in aqueous extract before and after incorporating metal nanoparticles (M-NPs). The efficiency of ZnO-P. nano-extract was studied on cell cycle and apoptosis of human hepatocellular carcinoma (HEPG-2) cells. Then, both Ag- and ZnO-P. nano-extracts were studied against hepatic fibrosis induced by thioacetamide (TAA) in rats through undergoing different hematological and biochemical measurements in addition to the histopathological examination in hepatic tissues compared to the extract itself. RESULTS: The ZnO-P. nano-extract showed significantly (P<0.05) higher antioxidant and scavenging activity due to the existence of higher total polyphenolic content. Also, it exhibited a significantly (P<0.05) higher inhibitory effect on acetyl cholinesterase (AChE) activity and higher cytotoxic activity against HEPG-2 cells. Therefore, ZnO-P. nano-extract was studied against the cell cycle and apoptosis of HEPG-2 cells compared to the extract itself. It was found that ZnO-P. nano-extract was safer than Ag-P. nano-extract. Both Ag- and ZnO- P. nano-extracts were studied against the hepatic fibrosis induced by thioacetamide (TAA) compared to the native extract. It was noticed that ZnO-P. nano-extract exhibited an ameliorative effect against hepatic fibrosis by decreasing levels of inflammatory and fibrotic markers significantly (P<0.05) more than Ag-P. nano-extract. Furthermore, it improved the antioxidant status of the hepatic tissue in addition to restoring the histopathological architecture of liver tissue. CONCLUSION: ZnO-P. nano-extract showed higher in vitro and in vivo biological activities than Ag-P. nano-extract and native P. extract itself.

Clinico-pathological assessment of surgically removed abdominal wall endometriomas following cesarean section.

BACKGROUND: Over the past few decades, the rate of Cesarean Section (CS) delivery has been rising rapidly and the prevalence of CS-associated complications including Abdominal Wall Endometriomas (AWE) increases with each additional operation. The aim of this study was to evaluate the clinical characteristics, histopathological diagnostic role and surgical management of post-CS AWE through a retrospective case review. METHODS: We calculated the incidence of AWE and reviewed all the patients underwent surgical removal of Post-CS AWE during the period of 2012-2018 who were diagnosed, treated and followed up for 2-8 years at our tertiary hospital. RESULTS: Thirty women with AWE were included. The main symptom in 2/3 of cases was cyclic pain and 4 cases (13.3%) had no symptoms. The mean interval between prior CS and appearance of symptoms was 55.2 months and the mean size of the excised mass was 42 mm. Free surgical margin was less than 9 mm in 9 patients (30%) but no recurrence was recorded among all the studied patients. Pre-operative FNAC diagnosis was performed for only 3 patients (10%) which helped in excluding other potential pathologies. The clinical-pathological agreement value for detection of the nature of the abdominal wall mass was 93.4%. CONCLUSIONS: Patients with suspected AWE should undergo preoperative cytological biopsy to exclude alternative diagnosis. Wide surgical excision with margin of less than 1 cm could be accepted especially in case of weak abdominal wall. More studies on the post-CS complications; risks, prevention, early detection and proper management should be encouraged.

Assessment, diagnosis and management of the dizzy patient.

Dizziness and balance disorders are very common problems. Having a structured approach, including adequate history taking and clinical examination, in a multidisciplinary environment allows for effective management of patients with these complex symptoms. Hearing assessment is an integral part of the assessment of patients with dizziness, along with the occasional need for further testing. Identifying red flags, along with the ability to involve different medical specialties, are prerequisites for safe management and a successful outcome. While surgical intervention has a small role in the management of balance disorders, vestibular rehabilitation remains the cornerstone of treatment, along with different supportive measures. This article outlines the approach used in the authors' centre to manage patients with dizziness and balance disorders.

Flood Hazard Assessment of the Urban Area of Tabuk City, Kingdom of Saudi Arabia by Integrating Spatial-Based Hydrologic and Hydrodynamic Modeling.

This study deals with the use of remote sensing (RS), geographic information systems (GISs), hydrologic modeling (water modeling system, WMS), and hydraulic modeling (Hydrologic Engineering Center River Analysis System, HEC-RAS) to evaluate the impact of flash flood hazards on the sustainable urban development of Tabuk City, Kingdom of Saudi Arabia (KSA). Determining the impact of flood hazards on the urban area and developing alternatives for protection and prevention measures were the main aims of this work. Tabuk City is exposed to frequent flash flooding due to its location along the outlets of five major wadis. These wadis frequently carry flash floods, seriously impacting the urban areas of the city. WMS and HEC-HMS models and RS data were used to determine the paths and morphological characteristics of the wadis, the hydrographic flow of different drainage basins, flow rates and volumes, and the expansion of agricultural and urban areas from 1998 to 2018. Finally, hydraulic modeling of the HEC-RAS program was applied to delineate the urban areas that could be inundated with floodwater. Ultimately, the most suitable remedial measures are proposed to protect the future sustainable urban development of Tabuk City from flood hazards. This approach is rarely used in the KSA. We propose a novel method that could help decision-makers and planners in determining inundated flood zones before planning future urban and agricultural development in the KSA.

Suitability estimation for urban development using multi-hazard assessment map.

Preparation of natural hazards maps are vital and essential for urban development. The main scope of this study is to synthesize natural hazard maps in a single multi-hazard map and thus to identify suitable areas for the urban development. The study area is the drainage basin of Xerias stream (Northeastern Peloponnesus, Greece) that has frequently suffered damages from landslides, floods and earthquakes. Landslide, flood and seismic hazard assessment maps were separately generated and further combined by applying the Analytical Hierarchy Process (AHP) and utilizing a Geographical Information System (GIS) to produce a multi-hazard map. This map represents the potential suitability map for urban development in the study area and was evaluated by means of uncertainty analysis. The outcome revealed that the most suitable areas are distributed in the southern part of the study area, where the landslide, flood and seismic hazards are at low and very low level. The uncertainty analysis shows small differences on the spatial distribution of the suitability zones. The produced suitability map for urban development proves a satisfactory agreement between the suitability zones and the landslide and flood phenomena that have affected the study area. Finally, 40% of the existing urban pattern boundaries and 60% of the current road network are located within the limits of low and very low suitability zones.

Assessment of cochlear and auditory pathways in patients with migraine.

PURPOSE: In this study, we aimed to determine the function of the cochlea and peripheral and central auditory pathways with migraine. MATERIALS AND METHODS: Fifty-eight patients with migraine and 40 healthy subjects were assessed using routine diagnostic audiometry along with transient evoked otoacoustic emissions (TOAEs), distortion product otoacoustic emissions (DPOAEs), and auditory brainstem response (ABR) at high and low repetition rate frequencies. RESULTS: Nearly two thirds of patients with migraine had one or more abnormalities in electrophysiological testing. Compared with control subjects, patients reported significant lowering of TOAEs amplitude at frequencies of 1 kHz (right: P = .0003; left: P = .002), 3 kHz (right: P = .025), and 4 kHz (right: P = .019); prolonged wave III latency (right: P = .009); and I-V interpeak latency (IPL) (left: P = .024) at high repetition rate frequencies. Significant correlations were identified between age, duration of illness and frequency of migraine and TOAEs total response and at amplitude of 4 kHz, amplitudes of DPOAEs at 1, 1.5, 2, 3, and 5 kHz and I, III and wave latencies and I-V IPL of ABR at high rate frequencies. CONCLUSIONS: These data suggest that subclinical changes in cochlear function and auditory pathways are associated with chronic migraine. It is possible that migraine could be accompanied by compromise of blood supply of auditory system.