Occurrence, distribution, and sources of organophosphate esters (OPEs) in the air of the Indo-China Peninsula Based on a Passive Air Monitoring Network.

Organophosphate esters (OPEs) are a class of emerging and ubiquitous contaminants that are attracting increasing attention, and their large-scale use as flame retardants and plasticizers has led to their pervasive presence in the environment, although their broader impacts remain unknown. In this study, 11 OPEs were measured in the atmosphere of Southeast Asia and Southwest China during 2016. The ∑11OPEs were higher in this region (78.0-1670 pg/m3, mean 458 pg/m3) than in many remote areas, lower than in developed regions, and comparable to levels in many developing country cities. Generally, the ∑11OPEs were higher in urban (105-1670 pg/m3, mean 538 pg/m3) than in suburban (78.0-1350 pg/m3, mean 388 pg/m3). Seasonal variations of OPEs in the air were more pronounced in Cambodia and Laos, especially for Triphenyl Phosphate (TPHP). Seasonal variations of ∑11OPEs in most regions correspond to changes in temperature and rainfall. Biomass burning may be also a factor in facilitating OPE emissions from biomass materials or soil into the atmosphere of Southeast Asia. The random forest analysis showed that among these, rainfall had the greatest effect on the seasonal variation of atmospheric OPE concentrations, followed by biomass burning and temperature. The inter-regional variation of ∑11OPEs in Southeast Asia was related to population and economic development in each region. Airflow trajectories indicated that the OPEs in this region were mainly from local sources. The health risk assessment revealed that the inhalation exposure risks of OPEs to the residents in the study areas were very low during the sampling period, but may be increasing.

Metabolic and immune costs balance during natural acclimation of corals in fluctuating environments.

Epigenetic modifications based on DNA methylation can rapidly improve the potential of corals to adapt to environmental pressures by increasing their phenotypic plasticity, a factor important for scleractinian corals to adapt to future global warming. However, the extent to which corals develop similar adaptive mechanisms and their specific adaptation processes remain unclear. Here, to reveal the regulatory mechanism by which DNA methylation improves thermal tolerance in Pocillopora damicornis under fluctuating environments, we analyzed genome-wide DNA methylation signatures in P. damicornis and compared the differences in the methylation and transcriptional responses of P. damicornis from fluctuating and stable environments using whole-genome bisulfite sequencing and nanopore-based RNA sequencingtranscriptome sequencing. We discovered low methylation levels in P. damicornis (average methylation 4.14%), with CpG accounting for 74.88%, CHH for 13.27%, and CHG for 11.85% of this methylation. However, methylation levels did not change between coral samples from the fluctuating and stable environments. The varied methylation levels in different regions of the gene revealed that the overall methylation level of the gene body was relatively high and showed a bimodal methylation pattern. Methylation occurs primarily in exons rather than introns within the gene body In P. damicornis, there was only a weak correlation between methylation and transcriptional changes at the individual gene level, and the methylation and gene expression levels generally exhibited a bell-shaped relationship, which we speculate may be due to the specificity of cnidarian species. Correlation analysis between methylation levels and the transcriptome revealed that the highest proportion of the top 20 enriched KEGG pathways was related to immunity. Additionally, P. damicornis collected from a high-temperature pool had a lower metabolic rate than those collected from a low-temperature pool. We hypothesize that the dynamic balance of energy-expenditure costs between immunity and metabolism is an important strategy for increasing P. damicornis tolerance. The fluctuating environment of high-temperature pools may increase the heat tolerance in corals by increasing their immunity and thus lowering their metabolism.

Bioaccumulation and trophic transfer of PAHs in tropical marine food webs from coral reef ecosystems, the South China Sea: Compositional pattern, driving factors, ecological aspects, and risk assessment.

Multiple environmental pressures caused by global warming and human activities have aroused widespread concern about PAHs pollution in tropical marine coral reef regions (CRRs). However, the trophodynamics of PAHs in the food webs of the CRRs and the related influence factors have not been reported. This study investigated the occurrence, trophic amplification, and transmission of PAHs in various organisms selecting between at least representative species for each level in CRRs of the South China Sea (SCS); revealed their driving mechanisms; and explored the trophodynamics of PAHs in the food web of the coral reef ecosystem. Results showed that more PAHs can be accumulated in the mantle tissue of Tridacnidae, and the proportion of mantle tissue of Tridacnidae increases with the increase of latitude (y = 0.01x + 0.17, R2 = 0.49, p < 0.05). Latitude drives the differential occurrence level and bioaccumulation of PAHs in tropical marine organisms, and also affects the trophodynamics of PAHs in aquatic ecosystem food webs. PAHs undergo trophic amplification in the food webs of tropical marine ecosystems represented by coral reefs, thus further aggravating the negative environmental impact on coral reef ecosystems. The cancer risk caused by accidental ingestion of PAHs by humans through consumption of seafood in CRRs is very low, but we should be alert to the biomagnification effect of PAHs.

Occurrence, distribution, source identification, and risk assessment of organophosphate esters in the coastal waters of Beibu Gulf, South China Sea: Impacts of riverine discharge and fishery.

As emerging pollutants, the environmental geochemistry of organophosphate esters (OPEs) in the coastal zone with multiple functional areas are still less recognized. This study investigated spatiotemporal distribution, sources and risks of 11 widely used OPEs in surface waters from seagoing rivers and multiple coastal functional areas of the Beibu Gulf. The results indicated that significantly higher ∑11OPEs (total concentrations of 11 OPEs, ng/L) occurred in summer (34.2-1227) than in winter (20.6-840), as a result of the high emission caused by climate reasons. In general, higher ∑11OPEs occurred in rivers (41.2-1227) than in the coast (34.2-809) in summer, especially in the urban rivers, while in winter, higher ∑11OPEs occurred in the coast (23.4-840 vs 20.6-319 in rivers) because of obviously higher ∑11OPEs in marine fishery areas (99-840). Source identification revealed that fishery activity, especially fishing vessels, and urban rivers were the main sources of OPEs in the Beibu Gulf. For the individual OPE, only tri-n-butyl phosphate (TNBP) may have ecological risks to aquatic organisms in a few sites, but if considering the additive effects, the OPEs mixtures would pose a high risk to algae and low to medium threats to crustaceans and fish.

Occurrence, distribution, sources, and bioaccumulation of polycyclic aromatic hydrocarbons (PAHs) in multi environmental media in estuaries and the coast of the Beibu Gulf, China: a health risk assessment through seafood consumption.

The coastal zone is a crucial transitional area between land and ocean, which is facing enormous pressure due to global climate change and anthropogenic activities. It is essential to pay close attention to the pollution caused by polycyclic aromatic hydrocarbons (PAHs) in the coastal environment and their effect on human health. The pollution status of PAHs was investigated in the Beibu Gulf, taking into consideration various environmental media. The results showed that the total concentration of 16 PAHs (Σ16PAHs) was significantly higher in winter than in summer. Compared to the coastal area, the status of PAHs in the estuarine areas was found to be more severe in summer, while the regional difference was insignificant in winter. In summer, the Σ16PAHs in estuarine waters (71.4 ± 9.58 ng/L) > coastal waters (50.4 ± 9.65 ng/L); estuarine sediment (146 ± 116 ng/g) > coastal zone (76.9 ± 108 ng/g). The source apportionment indicated that spilled oil, biomass, and coal burning were the primary sources of PAHs in the water. The predominant sources of pollution in the sediments were spilled oil, fossil fuel burning, and vehicle emissions. With regard to the status of PAHs in marine organisms in the coastal area of the Beibu Gulf, the highest average concentration of PAHs was indicated in shellfishes (183 ± 165 ng/g), followed by fishes (73.7 ± 57.2 ng/g), shrimps (42.7 ± 19.2 ng/g), and crabs (42.7 ± 19.2 ng/g) in Beibu Gulf coastal area. The calculated bioaccumulation factor indicates a low bioaccumulation capacity of PAHs in various seafood considering the ambient environment. The human health risk assessment considering multiple age groups indicates minimal health risk on accidental ingestion of PAHs through seafood. However, it is suggested that the intake of shellfish in children be controlled.

Occurrence, source apportionment and risk assessment of antibiotics in water and sediment from the subtropical Beibu Gulf, South China.

The widespread use of antibiotics has raised global concerns, but scarce information on antibiotics in the subtropical marine environment is available. In the present study, seawater and sediment samples were collected to investigate the occurrence, spatial distribution, source, and ecological risks of 22 antibiotics in the Beibu Gulf. The total concentrations of target antibiotics (∑antibiotics) were in the range of 1.74 ng/L to 23.83 ng/L for seawater and 1.33 ng/g to 8.55 ng/g dry weight (dw) for sediment. Spatially, a decreasing trend of antibiotic levels from coast to offshore area was observed, with relatively high levels at the sites close to the Qinzhou Bay and Qiongzhou Strait. Sulfamethoxazole (SMX), trimethoprim (TMP), and norfloxacin (NOX) were predominant in seawater, while NOX, enoxacin (ENX), and enrofloxacin (ENR) were the most abundant antibiotics in sediment. In general, the sediment-water partitioning coefficients (Kd) were positively correlated with log molecular weight (MW). Salinity, particle size, and pH of water were predicted to be vital factors influencing the partition of sulfadiazine (SDZ), CIX, and ENR (p < 0.05). Livestock and aquaculture were identified as dominant sources of antibiotics in the Beibu Gulf based on PCA-MLR and Unmix model. Risk assessment revealed that SMX, CIX could pose medium risks to algae in the Beibu Gulf. Overall, our results provided paramount insights into understanding the fate and transport behaviors of antibiotics in the subtropical marine environment.

Legacy and alternative per- and polyfluoroalkyl substances in a subtropical marine food web from the Beibu Gulf, South China: Fate, trophic transfer and health risk assessment.

The usage of alternative per- and polyfluoroalkyl substances (PFASs) has been increasing due to the restriction and elimination of legacy PFASs. However, there is limited knowledge on bioaccumulation and trophic magnification of alternative PFASs, especially in subtropical ecosystems. In the present study, we performed a comprehensive survey to investigate the occurrence, bioaccumulation and trophic magnification of legacy and alternative PFASs in subtropical marine food webs in the Beibu Gulf, South China. Results showed that perfluorobutanoic acid (PFBA) and perfluorooctanoic acid (PFOA) were the predominant PFASs in water phase, while perfluorooctane sufonate (PFOS) contributed most to the sum of target PFASs in sediments and marine organisms. Of the investigated PFASs, PFOS and 6:2 chlorinated polyfluoroalkyl ether sulfonic acids (F-53B) exhibited the highest bioaccumulation factor with values > 5000, qualifying as very bioaccumulative chemicals. There was a significant positive correlation between log BSAF and the carbon chain length of perfluoroalkyl carboxylic acids (PFCAs). Trophic magnification (TMF) was observed for PFOS and F-53B, while the remaining PFASs were biodiluted through the present food web. The hazard ratios for PFOS and PFOA in all organisms were far less than unity, suggesting overall low PFAS risks for humans through consumption of marine organisms.

Spatial distribution, source identification, and risk assessment of organochlorines in wild tilapia from Guangxi, South China.

Seventy-five wild tilapia samples from six rivers (ten sites) in Guangxi province were collected and analyzed for 53 organochlorine compounds. DDTs, endosulfan, and PCBs were the most dominant compounds found in this study. Tiandong County (TD) and Guigang City (GG) sites were found to be heavily contaminated with high levels of endosulfan (385-925 ng/g lw) and/or DDTs (20.1-422 ng/g lw). The diagnostic ratios indicated that the residues of DDTs and endosulfan in wild tilapia are associated with historical applications as well as the recent introduction of technical DDTs and endosulfan at some sampling sites. The correlation between total length, body mass, and organochlorines (OCs) was higher than the correlation between age and lipid content. There was no significant correlation between organochlorine pesticides (OCPs) and lipid content. Therefore, for organisms, the feeding intensity (related to length and mass) of fish could better reflect degree of pollution than exposure time (age) of fish. The hazardous ratios for the 50th and 95th percentile data of OCPs and PCBs in fish were both below 1, suggesting that daily exposure to OCPs and PCBs yields a lifetime cancer risk lower than 1 in 10,000.

Occurrence, phase distribution, and bioaccumulation of organophosphate esters (OPEs) in mariculture farms of the Beibu Gulf, China: A health risk assessment through seafood consumption.

As emerging pollutants, the occurrence and risks of organophosphate esters (OPEs) in mariculture farms should be concerned; however, information is limited. Beibu Gulf is one of the essential mariculture zones in China. This study aimed to investigate the occurrence of OPEs in mariculture farms of the Beibu Gulf, their phase distribution and bioaccumulation among sediment, organisms (shrimp, crab, and oyster), water, and feed. Human exposure to OPEs through seafood consumption was also assessed. The total concentrations of the 11 target OPEs (∑11OPEs) in the water samples ranged 32.9-227 ng L-1. It was significantly higher in water from the culture ponds (mean 122 ng L-1) than in water from the estuaries and nearshore areas (mean 51.1 ng L-1) (nonparametric test, p < 0.05). ∑11OPEs in the feeds averaged 46.0 (range 21.7-84.5) ng g-1 dw, which is similar to the level in the organism samples (mean 55.5, range 21.3-138 ng g-1 dw) and 4.4 times higher than that in the sediment (mean 10.9, range 35-22.1 ng g-1 dw). The ∑11OPEs released from the feeds to the culture ponds was estimated to be 49 µg m-2 per three-month period. In the aquaculture ponds, the sediment-water distribution coefficient (log KOC), and the bioaccumulation factors from the water (log BWAFs) or the feed (log BFAFs) to the organisms, depend linearly on the hydrophobicity (log KOW) of OPEs. The log BWAFs and log BFAFs increased with increasing log KOW within the log KOW range of 1-7. The human exposure to OPEs through consumption of shrimp, crab, and oysters from the mariculture farms does not pose a health risk at present.

Species-specific profiles and risk assessment of perfluoroalkyl substances in coral reef fishes from the South China Sea.

The contamination profiles of sixteen perfluoroalkyl substances (PFAS) were examined in coral reef fish samples collected from the South China Sea (SCS) where no information about this topic was available in the literature. The results revealed that six PFAS were found in coral reef fish samples from the SCS. Perfluorooctane sulfonate (PFOS) was the most predominant PFAS contaminant detected in most of the samples, with the highest concentration value of 27.05 ng/g wet weight (ww) observed in Cephalopholis urodelus. Perfluoroundecanoic acid (PFUnDA) and Perfluorotridecanoic acid (PFTrDA) were the second and third dominant PFAS, respectively. Mean PFOS concentrations in muscle of seven coral reef fish varied from 0.29 ng/g ww in Lethrinus olivaceus to 10.78 ng/g ww in Cephalopholis urodelus. No significant linear relationship was observed between PFOS levels and coral reef fish traits (length, weight) collected in this region. Average daily intake of PFOS for the seven coral reef fishes ranged from 0.79 ng/kg/d for Lethrinus olivaceus to 29.53 ng/kg/d for Cephalopholis urodelus. The hazard ratio (HR) values for human consumption of PFOS-contaminated coral reef fishes ranged from 0.04 to 1.48, with Cephalopholis urodelus having the highest HR value of 1.18 (higher than 1) among the species, indicating frequent consumption of Cephalopholis urodelus might pose potential health risk to local population. The present work have provided the first hand data of PFAS in coral reef fishes in the SCS and indirectly demonstrated the existence of low level PFAS pollution in the SCS in China.