Silk fibroin nanoparticles and ß-tricalcium phosphate loaded tissue engineered gelatin bone scaffolds: A Nature-based, low-cost solution.

Tissue engineering has recently attracted attention as an alternative to traditional treatment methods for tissue and organ damage. Since bone is one of the most important vital parts of the body, the treatment of bone damage is important. Silk fibroin is a natural polymer with properties such as biocompatibility and biodegradability, which attracts attention with its controlled release, especially in drug delivery systems. In this study, gelatin-based scaffolds loaded with silk fibroin nanoparticles and ß -tricalcium phosphate (ß -TCP) were developed to be used as a potential drug delivery system in bone tissue engineering. The chosen nanoparticle formulation has a 294 nm average diameter with a 0.380 polidispersity index (PDI). In vitro characterization of scaffolds was performed by mechanical, morphological characterization, swelling capacity, Differential Scanning Calorimetry (DSC), Fourier-Transform Infrared Spectroscopy (FT-IR) measurements, and biocompatibility was evaluated by cell culture studies. Swelling index, tensile strength, elongation at break, and Young modulus of the ß -TCP and silk nanoparticles loaded scaffold were found as 456%, 1.476 MPa, 6.75%, and 24 MPa, respectively. In vitro cell culture studies have shown that scaffolds prepared in the present study can accelerate osteoblast differentiation and increase the healing rate of bone tissues. In addition, they have the potential to be used as a drug delivery system in bone tissue engineering that needs to be evaluated with further studies.

Determination of selected endocrine disruptors in organic, free-range, and battery-produced hen eggs and risk assessment.

An increasing amount of evidence suggests that phthalic acid esters (PAE), polychlorinated biphenyls (PCB), polybrominated diphenyl ethers (PBDE), and organochlorine pesticides (OCP) are related to mutagenic, carcinogenic, and endocrine disruptor effects (EDCs). These lipophilic compounds are highly resistant to breakdown processes, and consequently remain in the environment, followed by uptake into the food chain. Human exposure to lipophilic compounds results from the consumption of food containing EDCs, mainly foodstuffs of animal origin with a high fat content, since these contaminants accumulate in fatty tissues. Foodstuffs in which EDCs can accumulate include meat, fish, eggs, and milk. We investigated the contamination in edible eggs to determine whether relative differences in the contaminants' residue levels appeared in three types of egg production (i.e., battery, free-range, and organic). The results showed that PAEs, especially dimethyl phthalate contamination, was the most abundant in the battery eggs, and the PCBs, PBDEs, and OCPs were the most abundant in the free-range eggs. The eggs were contaminated by more than one chemical, and as many as five contaminants (PCB180, PBDE47, dimethyl phthalate, diethyl phthalate, and di-n-butyl phthalate in battery eggs, and PCB138, PCB153, PCB180, diethyl phthalate, and di-2-ethylhexyl phthalate in organic eggs) were detected in the same egg. However, none of the chemicals detected were at the maximum limit of acceptable risk.