RESUMO
In this study we evaluated cloning efficiency of second-generation (G2) cloned Holstein cows derived from ear fibroblasts of a first-generation (G1) cloned cow, and assessed their health status in terms of physical, growth and reproductive parameters. Compared with G1 cloning, G2 cloning showed a slight decrease on blastocyst rate of reconstructed embryos (30.2±5.8% vs. 28.5±7.2%, p>0.05), while the quality of its blastocysts reduced significantly (Grade 1 and Grade 2, 21.1±4.1% vs. 17.1±5.7%, p<0.05). After embryo transfer (ET), both pregnancy rate to term and calving rate of G2 cloning were approximately half of G1 cloning (5.8% vs. 10.7%; 3.9% vs. 8.6%, p>0.05). Six G2 cloned cows were delivered, and three of them survived. G2 cloned calves displayed symptoms of being overweight at birth and tachycardia in the first week after birth. During the first 12 months, the growth of G2 cloned calves was similar to control calves derived from artificial insemination (AI). Furthermore, the interindividual variation of growth within the G2 clonal family was smaller except at birth and at two months of age. Interestingly, although G2 cloned cows reached puberty 45 days later in comparison with control cows derived from AI, they were all pregnant by AI, and gave birth to healthy calves. This suggests that their reproductive performance was not affected by late puberty. In summary, our results showed that although cloning efficiency of G2 was lower than that of G1, the surviving G2 clones appeared physically healthy and were fertile.