RESUMO
Infection and epithelial downgrowth are two major problems with maxillofacial transcutaneous implants, and both are mainly due to lack of stable closure of soft tissues at transcutaneous sites. Fibroblasts have been shown to play a key role in the formation of biological seals. In this work, titanium (Ti) model surfaces were coated with mussel adhesive proteins (MAPs) utilizing its unique adhesion ability on diverse inorganic and organic surfaces in wet environments. Prepared basic fibroblast growth factor (bFGF)-poly(lactic-co-glycolic acid) (PLGA) microspheres can be easily synthesized and combined onto MAPs-coated Ti surfaces, due to the negative surface charges of microspheres in aqueous solution, which is in contrast to the positive charges of MAPs. Titanium model surfaces were divided into three groups. Group A: MAPs/bFGF-PLGA microspheres composite-coated Ti surfaces. Group B: MAPs-coated Ti surfaces. Group C: uncoated Ti surfaces. The effects of coated Ti surfaces on adhesion of fibroblasts, cytoskeletal organization, proliferation, and extracellular matrix (ECM)-related gene expressions were examined. The results revealed increased adhesion (P < 0.05), enhanced actin cytoskeletal organization, and up-regulated ECM-related gene expressions in groups A and B compared with group C. Increased proliferation of fibroblasts during five days of incubation was observed in group A compared with groups B and C (P < 0.05). Collectively, the results from this in vitro study demonstrated that MAPs/bFGF-PLGA microspheres composite-coated Ti surfaces had the ability to increase fibroblast functionality. In addition, MAPs/bFGF-PLGA microsphere composite-coated Ti surfaces should be studied further as a method of promoting formation of stable biological seals around transcutaneous sites.
Assuntos
Fator 2 de Crescimento de Fibroblastos/farmacologia , Fibroblastos/citologia , Fibroblastos/fisiologia , Ácido Láctico/farmacologia , Ácido Poliglicólico/farmacologia , Proteínas/farmacologia , Titânio/farmacologia , Animais , Bivalves/química , Cápsulas/administração & dosagem , Cápsulas/síntese química , Adesão Celular/efeitos dos fármacos , Adesão Celular/fisiologia , Proliferação de Células/efeitos dos fármacos , Proliferação de Células/fisiologia , Células Cultivadas , Materiais Revestidos Biocompatíveis/síntese química , Materiais Revestidos Biocompatíveis/farmacologia , Resinas Compostas/farmacologia , Fator 2 de Crescimento de Fibroblastos/química , Fibroblastos/efeitos dos fármacos , Humanos , Ácido Láctico/química , Microesferas , Organização e Administração , Ácido Poliglicólico/química , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Proteínas/química , Propriedades de Superfície , Titânio/químicaRESUMO
We describe a new osteotomy for transport of a disc of alveolar bone, which can simultaneously close an oronasal fistula with two layers of mucoperiosteum during distraction osteogenesis for reconstruction of a maxillary defect.
Assuntos
Alveoloplastia/métodos , Doenças Maxilares/cirurgia , Doenças Nasais/cirurgia , Fístula Bucal/cirurgia , Osteogênese por Distração/métodos , Osteotomia/métodos , Procedimentos de Cirurgia Plástica/métodos , Fístula do Sistema Respiratório/cirurgia , Processo Alveolar/patologia , Humanos , Maxila/patologia , Mucosa Bucal/cirurgia , Palato/patologia , Periósteo/cirurgiaRESUMO
This clinical report presents a novel approach that integrates a new optical digitizing (scanning) technique, a three-dimensional ocular prostheses database, and the Selective Laser Sintering technique to achieve the computer-aided design and manufacture of an orbital prosthesis. An optical-structured light scanner was used to develop a color digital model of the unaffected orbital contour, which was copied and then mirrored to generate the orbital prosthesis contour data. The ocular prostheses database was applied to ascertain the size and position of the eyeball within the orbital prosthesis. Then, a Selective Laser Sintering machine directly manufactured the wax pattern of the definitive orbital prosthesis from the three-dimensional orbital data. This new approach is time and cost-effective and can be considered an alternative to traditional manual techniques of creating facial prostheses.