Determination of heavy metals from Aloe vera by- product in golden mullet (Liza aurata); A consumer health risk assessment.

Heavy metals have become subject of concern in the recent years because of its potency to cause cardiovascular diseases and other toxic health effects. Therefore, this research was assumed to investigate the level of toxicity in terms of heavy metals accumulation in the fish samples and its benefits and risk for human consumers health and also evaluate the partial replacement of plant sources by canarian Aloe vera diets as a pure product or like a by-product on toxicological effects on the golden mullet (Liza aurata) fillet and whole body. In this study risks arising from fish metal content has been measured using various parameters as Estimated Daily Intake (EDI), Maximum Safe Consumption (MSCA), Target Hazard quotient (THQ), Hazard Index (HI) Carcinogenic risk of As (As- CR), the Value Selenium Health Benefit (Se HBV) and also the Nutritional Values has been evaluated. The results showed that all heavy metal levels in the fish tissue and diets were below the confirmed safe limits for consumption. In case of diets, it is obvious that with the exception of As, Hg, and Se, the presence of heavy or essential metals in both whole fish and raw fillet in golden grey mullet given experimental diets revealed that the whole fish had the highest concentration. Thus, it can be concluded that Aloe vera product and byproduct were in safety limits for fish and also for humans through food chain. Various risk and benefit assessment measures established by national and international authorities concluded that Liza aurata use was mostly safe.

Assessment of Aloe vera (L.) genotoxic potential on Escherichia coli and plasmid DNA.

Aloe vera is a tropical plant, known in Brazil as babosa and several reputable suppliers produce a stabilized aloe gel for topic use. Since people use Aloe vera topically, they could be exposed to solar ultraviolet light in addition and it might cause a cross damage effect between these agents. The aim of this work was to investigate the biological effects of Aloe vera pulp extract, associated or not to UVA radiation, on Escherichia coli-deficient repair mutants and plasmid DNA, in order to test its genotoxic potential. Data obtained from analysis of survival fractions, bacterial transformation and agarose gel electrophoresis suggest that Aloe vera has genotoxic properties, but it seems not to be able to damage the cell membrane.