RESUMO
This study is the first to show that Hazard Analysis by Critical Control Point (HACCP)-based monitoring can be an effective tool for ensuring Salmonella-safe feed, by virtually eliminating feedborne Salmonella infection even in broiler production. Data from the control of Salmonella in feed and food animal production during 1982-2005, showed that conventional endpoint testing in feed mills did not ensure a Salmonella-safe feed, and in one feed mill failed to detect Salmonella contamination, resulting in the feed infecting 80 out of 197 (40.6%) recipient broiler flocks. Following implementation in 1991 of a HACCP-based control in feed mills, the annual number of samples tested at specified critical control points during a 15-year period increased from â¼4400 to 10,000, while the proportion of Salmonella-contaminated samples decreased from 2.0% to 0.3%. Thus, introduction of HACCP was followed by a dramatic decrease, from 40 to <5, in the annual number of Salmonella-infected broiler flocks identified by preslaughter monitoring. Incidence has generally remained at that low level, despite production since 1980 increasing from 39 to 112 million chickens per year. Feed mills start using soymeal with an unsafe Salmonella status and possibly with a suboptimal HACCP control, increased their level of Salmonella-contaminated HACCP samples, and their feed subsequently infected 78 swine-producing herds. The results also show that the HACCP concept can be an effective tool to supply feed mills with Salmonella-safe feed ingredients as demonstrated for a soybean crushing plant, which produced Salmonella-safe soymeal over a 19-year period despite frequent (34%) and highly varied (92 different serovars) Salmonella contamination in samples from incoming soybean. Similar results are reported for a plant producing rapeseed meal. It is emphasized that the achievements described through use of the HACCP required interventions of relevant preventive biosecurity measures and corrective actions when the HACCP-based monitoring identified Salmonella contamination.
Assuntos
Galinhas , Salmonelose Animal , Animais , Suínos , Análise de Perigos e Pontos Críticos de Controle , Suécia , Ração Animal , Salmonella , Salmonelose Animal/epidemiologia , Salmonelose Animal/prevenção & controleRESUMO
Ensuring the chemical, physical, and microbial safety of food and ingredients underpins the international trade of food items and is integral to building consumer confidence. Achieving this requires effective systems to support the safety of food across the supply chain. Differing risk-assessment approaches are utilized globally for establishing food safety systems, and bench marking these approaches against international food safety standards can assist in the development of country-specific systems. This China-Australia collaborative review examined similarities and differences in the food safety risk-assessment systems of China, Australia/New Zealand, Canada, and the United States, with the view to identify areas that could support improvements to the Chinese system. Key differences include the level of cohesiveness among stakeholders and the level to which each country promotes the international harmonization of standards. The evidence highlights a need for greater capacity-building in risk assessment in China that may support greater stakeholders' cohesion, improve hazard identification, and allow regulators to more readily keep abreast of changes to international standards. This review may help the Chinese food industry to replicate the same level of food safety risk assessment currently applied by other key countries, and reflects the determination, government prioritization, and active strengthening of China's National Centre for Food Safety Risk Assessment currently underway.
Assuntos
Comércio , Análise de Perigos e Pontos Críticos de Controle , Estados Unidos , Nova Zelândia , Internacionalidade , Inocuidade dos Alimentos , China , Medição de Risco , CanadáRESUMO
Abstract The present work reports the implementation of the Hazard Analysis Critical Control Point (HACCP) methodology to analyze the water purification system of a pharmaceutical site, in order to assure the system quality and prevent failures. As a matter of fact, the use of HACCP for development and implementation of Quality Risk Management (QRM) is not usual in pharmaceutical plants and it is applied here to improve the performance of the water purification system of a polymerization pilot plant used to manufacture pharmaceutical grade polymer microparticles. Critical Control Points (CCP) were determined with the aid of a decision tree and questions were made to characterize whether identified hazards constitute actual CCPs and should be monitored. When deviations were detected, corrective actions were performed and action plans were used for following-up and implementation of corrective actions. Finally, microbiological and physicochemical parameters were analyzed and the obtained results were regarded as appropriate. Therefore, it is shown that HACCP constitutes an effective tool for identification of hazards, establishment of corrective actions and monitoring of the critical control points that impact the process and the quality of the final pharmaceutical product most significantly.
Assuntos
Gestão de Riscos/classificação , Purificação da Água/instrumentação , Análise de Perigos e Pontos Críticos de Controle/métodos , Monitoramento Ambiental/instrumentação , Gestão da Qualidade Total/métodos , Indústria Farmacêutica/classificação , Metodologia como Assunto , Relatório de PesquisaRESUMO
Refined red palm-pressed mesocarp olein (PPMO) is recovered from palm-pressed mesocarp fiber, which is a by-product from palm oil mill. Its utilization in food industry is extremely limited even though it contains various phytonutrients. Thus, this study aimed to evaluate its toxicity effects by using the male Sprague-Dawley rat model. The rats were administered with a single dose of 2 g/kg PPMO in an acute toxicity study while administered with 2, 1, or 0.5 g/kg PPMO daily for 28 days in a sub-chronic toxicity study. The mortality, oral LD50 value, clinical observation, body and organ weight, hematological and biochemical analyses, pathological and histopathological examinations were assessed. The overall outcomes indicated that PPMO is non-toxic up to 2 g/kg and considered safe to be used in food application, especially as functional food ingredient and supplement attributed to its phytonutrients. Besides, this study provides an insight in alternative utilization of the wastes from palm oil mill.
Assuntos
Análise de Perigos e Pontos Críticos de Controle/métodos , Óleo de Palmeira/toxicidade , Testes de Toxicidade Aguda/métodos , Testes de Toxicidade Crônica/métodos , Animais , Peso Corporal/efeitos dos fármacos , Suplementos Nutricionais , Inocuidade dos Alimentos , Alimento Funcional , Dose Letal Mediana , Masculino , Tamanho do Órgão/efeitos dos fármacos , Óleo de Palmeira/administração & dosagem , Óleo de Palmeira/química , Compostos Fitoquímicos , Ratos Sprague-Dawley , Resíduos SólidosRESUMO
Fumonisin mycotoxins are a persistent challenge to human and livestock health in tropical and sub-tropical maize cropping systems, and more efficient methods are needed to reduce their presence in food systems. We constructed a novel, low-cost device for sorting grain, the "DropSort", and tested its effectiveness on both plastic kernel models and fumonisin-contaminated maize. Sorting plastic kernels of known size and shape enabled us to optimize the sorting performance of the DropSort. The device sorted maize into three distinct fractions as measured by bulk density and 100-kernel weight. The level of fumonisin was lower in the heaviest fractions of maize compared to the unsorted samples. Based on correlations among fumonisin and bulk characteristics of each fraction, we found that light fraction 100-kernel weight could be an inexpensive proxy for unsorted fumonisin concentration. Single kernel analysis revealed significant relationships among kernel fumonisin content and physical characteristics that could prove useful for future sorting efforts. The availability of a low-cost device (materials~USD 300) that can be used to reduce fumonisin in maize could improve food safety in resource-limited contexts in which fumonisin contamination remains a pressing challenge.
Assuntos
Contaminação de Alimentos/análise , Microbiologia de Alimentos , Fumonisinas/análise , Fusarium/química , Análise de Perigos e Pontos Críticos de Controle/métodos , Zea mays/química , Zea mays/microbiologia , Produtos Agrícolas/química , Produtos Agrícolas/microbiologia , Doenças das Plantas/microbiologia , TexasRESUMO
The COVID-19 pandemic has disrupted economies and societies throughout the world since early 2020. Education is especially affected, with schools and universities widely closed for long periods. People under 25 years have the lowest risk of severe disease but their activities can be key to persistent ongoing community transmission. A challenge arose for how to provide education, including university level, without the activities of students increasing wider community SARS-CoV-2 infections. We used a Hazard Analysis of Critical Control Points (HACCP) framework to assess the risks associated with university student activity and recommend how to mitigate these risks. This tool appealed because it relies on multiagency collaboration and interdisciplinary expertise and yet is low cost, allowing rapid generation of evidence-based recommendations. We identified key critical control points associated with university student' activities, lifestyle, and interaction patterns both on-and-off campus. Unacceptable contact thresholds and the most up-to-date guidance were used to identify levels of risk for potential SARS-CoV-2 transmission, as well as recommendations based on existing research and emerging evidence for strategies that can reduce the risks of transmission. Employing the preventative measures we suggest can reduce the risks of SARS-CoV-2 transmission among and from university students. Reduction of infectious disease transmission in this demographic will reduce overall community transmission, lower demands on health services and reduce risk of harm to clinically vulnerable individuals while allowing vital education activity to continue. HACCP assessment proved a flexible tool for risk analysis in a specific setting in response to an emerging infectious disease threat. Systematic approaches to assessing hazards and risk critical control points (#HACCP) enable robust strategies for protecting students and staff in HE settings during #COVID19 pandemic.
Assuntos
COVID-19/epidemiologia , Análise de Perigos e Pontos Críticos de Controle , Estudantes , Universidades , COVID-19/prevenção & controle , COVID-19/virologia , Humanos , Pandemias , SARS-CoV-2/isolamento & purificaçãoRESUMO
Deoxynivalenol (DON) is one of the most commonly found mycotoxins across the world, and it mainly contaminates staple food crops. This study aims to evaluate the dietary exposure of DON and to provide a geographical profile of DON exposure in China. The concentrations of DON and its acetylated derivatives in 15,004 cereal samples (10,192 wheat flour, 1750 maize meal, 892 oat flakes, and 2170 polished rice) were collected from 30 provinces, autonomous regions, or municipalities across China during 2010-2017, through a national food safety risk surveillance system. The consumption data for cereals were obtained from China National Nutrition and Health Survey in 2002, and 67,923 respondents from the same 30 regions were included in the analysis. Among all the cereals considered, the concentration was the highest in wheat flour, with the mean concentration of 250.8 µg/kg. Applying a worst-case scenario, some individuals were possibly at risk, but the probability of acute effects was low. The mean and median exposure for the entire population was 0.61 and 0.36 µg/kg bw/day, respectively, below the (PM) TDI, indicating an acceptable overall health risk in Chinese population. Wheat contributed to 86% of the total DON exposure. Significant discrepancy was observed between the exposure and the contamination of DON. The high-exposure cluster area was in northern China, whilst the most seriously contaminated regions were all located in the southeast, which formed a seriously contaminated area.
Assuntos
Exposição Ambiental/análise , Contaminação de Alimentos/análise , Micotoxinas/análise , Tricotecenos/análise , Acetilação , Adolescente , Adulto , Criança , Pré-Escolar , China , Exposição Dietética/análise , Grão Comestível , Análise de Perigos e Pontos Críticos de Controle , Humanos , Limite de Detecção , Pessoa de Meia-Idade , Vigilância da População , Vigilância em Saúde Pública , Medição de Risco , Tricotecenos/química , Adulto JovemRESUMO
ABSTRACT: Food safety is a current challenge that needs to be addressed globally to overcome burden of foodborne diseases. In this study, food samples collected from Pakistan Institute of Medical Sciences, Islamabad, were analyzed for microbial quality. Parameters to measure the presence or absence of Salmonella, Staphylococcus, coliform, fungi, enteropathogenic Escherichia coli, and total viable counts in foods were studied. Enumeration of aerobic mesophilic bacteria and coliform bacteria was carried out to check the quality of water. The results showed that there was microbial contamination in the foods served at hospital under investigation for this study. Most of the contamination existed because of nonhygienic practices by individuals and serving places. Salmonella, fecal coliforms, and fungal cross-contamination was reported. A hazard analysis and critical control point system was implemented to study what areas are at greater risk and are a reason of contamination in the hospital. The study concluded that high prevalence of the microbial contamination was observed in facilities of the Pakistan Institute of Medical Sciences hospital, requiring strict preventive and precautionary measures to be taken to ensure the safety and health of patients and attendants in the hospital.
Assuntos
Microbiologia de Alimentos , Análise de Perigos e Pontos Críticos de Controle , Contagem de Colônia Microbiana , Contaminação de Alimentos/análise , Hospitais , Humanos , Paquistão , SalmonellaRESUMO
BACKGROUND: Human dietary exposure to chemicals can result in a wide range of adverse health effects. Some substances might cause non-communicable diseases, including cancer and coronary heart diseases, and could be nephrotoxic. Food is the main human exposure route for many chemicals. We aimed to assess human dietary exposure to a wide range of food chemicals. METHODS: We did a total diet study in Benin, Cameroon, Mali, and Nigeria. We assessed 4020 representative samples of foods, prepared as consumed, which covered more than 90% of the diet of 7291 households from eight study centres. By combining representative dietary surveys of countries with findings for concentrations of 872 chemicals in foods, we characterised human dietary exposure. FINDINGS: Exposure to lead could result in increases in adult blood pressure up to 2·0 mm Hg, whereas children might lose 8·8-13·3 IQ points (95th percentile in Kano, Nigeria). Morbidity factors caused by coexposure to aflatoxin B1 and hepatitis B virus, and sterigmatocystin and fumonisins, suggest several thousands of additional liver cancer cases per year, and a substantial contribution to the burden of chronic malnutrition in childhood. Exposure to 13 polycyclic aromatic hydrocarbons from consumption of smoked fish and edible oils exceeded levels associated with possible carcinogenicity and genotoxicity health concerns in all study centres. Exposure to aluminium, ochratoxin A, and citrinin indicated a public health concern about nephropathies. From 470 pesticides tested across the four countries, only high concentrations of chlorpyrifos in smoked fish (unauthorised practice identified in Mali) could pose a human health risk. INTERPRETATION: Risks characterised by this total diet study underscore specific priorities in terms of food safety management in sub-Saharan Africa. Similar investigations specifically targeting children are crucially needed. FUNDING: Standards and Trade Development Facility.
Assuntos
Dieta/estatística & dados numéricos , Exposição Dietética/efeitos adversos , Poluentes Ambientais/efeitos adversos , Análise de Alimentos , Análise de Perigos e Pontos Críticos de Controle , Benin , Camarões , Humanos , Mali , NigériaRESUMO
Fish production systems in North-western Nigeria have a significant role in food, nutrition and income generations to families, yet an important setup for zoonotic disease transmission. The aim of this study was to provide a broad knowledge of the structure, activities and food safety risks of the fish value chains operating in North-western Nigeria using the value chain framework. A total of 16 focus groups and 8 key informant interviews were conducted to gather data from fish producers, fish sellers and fish processors in selected peri-urban and rural settlements in Kaduna State. In addition, 129 semi-structured questionnaires and observation checklists were used in this study to gather evidence-based data, such as demography of value chain actors, product characterization, and food safety risks. The fish value-chain in North-western Nigeria is characterised by four main stakeholders, namely, fish producers, transporters, as well as raw- and processed-fish-sellers (wholesalers, retailers). Two major sources were identified supplying farmed fish to North-western Nigeria: the distributors from the central and southern part of the country and the fish farmers within the North-western and North-eastern regions of Nigeria. Raw-fish-wholesalers within the two major markets sold most of their high-value products to raw-fish-retailers, while low-value products were routed to raw-fish-retailers in the rural communities, processed-fish-retailers and household consumers in rural settlements. There were no large companies operating and no differentiation of chains between aquaculture and wild fishery. Raw fishes not sold and began to rot were sold to street vendors at a cheaper price and household consumers. Fish production and supply chains were characterised by poor structural and sanitary support for food safety and hygiene measures. Food safety risks identified were related to lack of biosecurity measures in fish farms, lack of cold chain and access to running water, poor hygiene practices by all handlers, lack of fish inspection at all levels, lack of use of protective clothing and limited health inspection of handlers. In overall, government control of activities in fish value chains was relatively poor leading to the absence of food safety regulatory enforcement characterised by lack of institutional goals on improving food safety measures in a chain-wide distribution. Hence, this study points the significant structural, sanitary and hygiene limitations along the fish value chain components. It provides a baseline for microbial food safety risk assessments, and information required for policy-making regarding implementation of disease control programmes, as the sector is fast-evolving in Sub-Saharan Africa.
Assuntos
Aquicultura/estatística & dados numéricos , Peixes , Análise de Perigos e Pontos Críticos de Controle , Marketing/estatística & dados numéricos , Animais , Aquicultura/economia , Aquicultura/métodos , Inocuidade dos Alimentos , Análise de Perigos e Pontos Críticos de Controle/métodos , Marketing/economia , Marketing/métodos , NigériaRESUMO
The objective of this study was to develop a generic risk management system based on the Hazard Analysis and Critical Control Point (HACCP) principles for the prevention of critical negative energy balance (NEB) in dairy herds using an expert panel approach. In addition, we discuss the advantages and limitations of the system in terms of implementation in the individual dairy herd. For the expert panel, we invited 30 researchers and advisors with expertise in the field of dairy cow feeding and/or health management from eight European regions. They were invited to a Delphi-based set-up that included three inter-correlated questionnaires in which they were asked to suggest risk factors for critical NEB and to score these based on 'effect' and 'probability'. Finally, the experts were asked to suggest critical control points (CCPs) specified by alarm values, monitoring frequency and corrective actions related to the most relevant risk factors in an operational farm setting. A total of 12 experts (40 %) completed all three questionnaires. Of these 12 experts, seven were researchers and five were advisors and in total they represented seven out of the eight European regions addressed in the questionnaire study. When asking for suggestions on risk factors and CCPs, these were formulated as 'open questions', and the experts' suggestions were numerous and overlapping. The suggestions were merged via a process of linguistic editing in order to eliminate doublets. The editing process revealed that the experts provided a total of 34 CCPs for the 11 risk factors they scored as most important. The consensus among experts was relatively high when scoring the most important risk factors, while there were more diverse suggestions of CCPs with specification of alarm values and corrective actions. We therefore concluded that the expert panel approach only partly succeeded in developing a generic HACCP for critical NEB in dairy cows. We recommend that the output of this paper is used to inform key areas for implementation on the individual dairy farm by local farm teams including farmers and their advisors, who together can conduct herd-specific risk factor profiling, organise the ongoing monitoring of herd-specific CCPs, as well as implement corrective actions when CCP alarm values are exceeded.
Assuntos
Bovinos/fisiologia , Indústria de Laticínios/métodos , Análise de Perigos e Pontos Críticos de Controle , Gestão de Riscos/métodos , Bem-Estar do Animal , Animais , Europa (Continente) , FemininoRESUMO
BACKGROUND: The safety assessment and control of stacked transgenic crops is increasingly important due to continuous crop development and is urgently needed in China. The genetic stability of foreign genes and unintended effects are the primary problems encountered in safety assessment. Omics techniques are useful for addressing these problems. The stacked transgenic maize variety 12-5 × IE034, which has insect-resistant and glyphosate-tolerant traits, was developed via a breeding stack using 12-5 and IE034 as parents. Using 12-5 × IE034, its parents (12-5 and IE034), and different maize varieties as materials, we performed proteomic profiling, molecular characterization and a genetic stability analysis. RESULTS: Our results showed that the copy number of foreign genes in 12-5 × IE034 is identical to that of its parents 12-5 and IE034. Foreign genes can be stably inherited over different generations. Proteomic profiling analysis found no newly expressed proteins in 12-5 × IE034, and the differences in protein expression between 12 and 5 × IE034 and its parents were within the range of variation of conventional maize varieties. The expression levels of key enzymes participating in the shikimic acid pathway which is related to glyphosate tolerance of 12-5 × IE034 were not significantly different from those of its parents or five conventional maize varieties, which indicated that without selective pressure by glyphosate, the introduced EPSPS synthase is not has a pronounced impact on the synthesis of aromatic amino acids in maize. CONCLUSIONS: Stacked-trait development via conventional breeding did not have an impact on the genetic stability of T-DNA, and the impact of stacked breeding on the maize proteome was less significant than that of genotypic differences. The results of this study provide a theoretical basis for the development of a safety assessment approach for stacked-trait transgenic crops in China.
Assuntos
Variação Genética , Melhoramento Vegetal , Plantas Geneticamente Modificadas , Zea mays/genética , China , Inocuidade dos Alimentos , Dosagem de Genes , Instabilidade Genômica , Análise de Perigos e Pontos Críticos de Controle , ProteômicaRESUMO
Human breastmilk is a mixture of nutrients, hormones and bioactive molecules that are vital for infant growth and development. Infant formula (IF) lacks many of these compounds, most notably human milk oligosaccharides (HMOs), which are abundant in breastmilk but scarce in IF. Sialyllactoses, such as 3'-sialyllactose, constitute a large portion of the HMO fraction. To produce IF that matches breastmilk more closely, biosynthesized human-identical milk oligosaccharides (structurally identical to HMOs) such as 3'-sialyllactose sodium salt (3'-SL) are proposed for use in IF and foods for the general population. The safety assessment of 3'-SL comprised in vitro genotoxicity tests and a 90-day oral (gavage) toxicity study. This is the first 90-day study conducted with 3'-SL using neonatal rats (7 days old at the start of dosing-equivalent age to newborn human infants in terms of central nervous system and reproductive development), demonstrating the safety of 3'-SL for consumption by infants, the most sensitive age group. The neonatal rats received 3'-SL at doses up to 5,000 mg/kg body weight (BW)/day and reference controls received 5,000 mg/kg BW/day of fructooligosaccharide (an ingredient approved for use in IF) for comparison with the high-dose 3'-SL group, followed by a 4-week recovery period. There was no evidence of genotoxicity in vitro. In the absence of any test item-related adverse effects in the 90-day study, the high dose (5,000 mg/kg BW/day) was established as the no-observed-adverse-effect level. This confirms the safety of 3'-SL for use in IF for infants, as well as in functional foods for the general population.
Assuntos
Análise de Perigos e Pontos Críticos de Controle/métodos , Fórmulas Infantis/química , Fórmulas Infantis/toxicidade , Leite Humano/química , Testes de Mutagenicidade/métodos , Oligossacarídeos/química , Oligossacarídeos/toxicidade , Adulto , Animais , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Modelos Animais , RatosRESUMO
Kresoxim-methyl is a high-efficiency and broad-spectrum fungicide used for the control of rice fungal diseases; however, its residues after application potentially threaten human health. Investigations on the dissipation of kresoxim-methyl residue in rice field systems and dietary risk assessment of kresoxim-methyl in humans are limited. The present study employed the QuEChERS-GC-MS/MS method for residue analysis of kresoxim-methyl in rice plants, brown rice, and rice husks. The samples were extracted with acetonitrile and purified by PSA, C18 column, and GCB. The average recovery of the spiked target compounds in the three matrices was between 80.5% and 99.3%, and the RSD was between 2.1% and 7.1%. The accuracy and precision of the method is in accordance with the requirements of residue analysis methods. Dissipation dynamic testing of kresoxim-methyl in rice plants indicated a half-life within the range of 1.8â»6.0 days, and a rapid dissipation rate was detected. Dietary intake risk assessment showed that the national estimated daily intake (NEDI) of kresoxim-methyl in various Chinese subpopulations was 0.022â»0.054 µg/(kg bw·days), and the risk quotient (RQ) was 0.0000055â»0.00014%. These findings indicate that the risk for chronic dietary intake of kresoxim-methyl in brown rice is relatively low. The present study provides information and theoretical basis for guiding the scientific use of kresoxim-methyl in rice fields and evaluating its dietary risk in brown rice.
Assuntos
Contaminação de Alimentos/análise , Oryza/química , Resíduos de Praguicidas/análise , Estrobilurinas/análise , Análise de Perigos e Pontos Críticos de Controle , Resíduos de Praguicidas/isolamento & purificação , Reprodutibilidade dos Testes , Medição de Risco , Estrobilurinas/isolamento & purificaçãoRESUMO
The challenge of the present comprehensive toxicological study was to evaluate water, mechanical and thermal processing factors (PFs) of twenty four pesticides (acetamiprid, alphacypermethrin, azoxystrobin, boscalid, bupyrimate, chlorpyrifos, chlorothalonil, cyprodinil, deltamethrin, difenoconazole, fenazaquin, fenhexamid, fludioxonil, folpet, iprodione, lambdacyhlothrin, metalaxyl, pirymicarb, propargite, pyraclostrobin, tetraconazole, tiophanate methyl, thiram, trifloxystrobin) in different fruit and vegetables and estimate health risk for adults and children. The water (PFâ¯=â¯0,09-0,94), mechanical (PFâ¯=â¯0,13-0,32) and thermal (PFâ¯=â¯0,02-0,57) technology significantly or completely reduced concentrations of twenty one active substances in broccoli, tomatoes, strawberries and black currants. Pyrethroid insecticides (alphacypermethrin, deltamethrin and lambdacyhalothrin) exhibited PF above one in berries influenced by high temperatures. Comprehensive processing factor database technology/pesticide/matrix (over 160 PFs) for 24 pesticides in selected fruit and vegetable species after different processing treatments was created. This paper for the first time compares health risk assessments of acute and chronic of two subpopulations of adults and the most critical group of small children using two mathematical models, without (I) and with including (II) calculated PF values. More realistic estimation of the dietary intakes of the pesticides was achieved using PFs. The hazard quotients (HQs) estimated from chronic and acute dietary exposure (Model l) were above 20% and after intake correction (Model II) were reduced to HQsâ¯=â¯11.5% after water, 3% mechanical and 9.5% thermal treatment.
Assuntos
Contaminação de Alimentos/análise , Manipulação de Alimentos/métodos , Frutas/química , Análise de Perigos e Pontos Críticos de Controle/métodos , Resíduos de Praguicidas/análise , Verduras/química , Bases de Dados Factuais , Humanos , Medição de RiscoRESUMO
Staphylococcus species are a major pathogen responsible for nosocomial infections and foodborne illnesses. We applied a laser-based BARDOT (bacterial rapid detection using optical scattering technology) for rapid colony screening and detection of Staphylococcus on an agar plate and differentiate these from non-Staphylococcus spp. Among the six growth media tested, phenol red mannitol agar (PRMA) was found most suitable for building the Staphylococcus species scatter image libraries. Scatter image library for Staphylococcus species gave a high positive predictive value (PPV 87.5-100%) when tested against known laboratory strains of Staphylococcus spp., while the PPV against non-Staphylococcus spp. was 0-38%. A total of nine naturally contaminated bovine raw milk and ready-to-eat chicken salad samples were tested, and BARDOT detected Staphylococcus including Staphylococcus aureus with 80-100% PPV. Forty-five BARDOT-identified bacterial isolates from naturally contaminated foods were further confirmed by tuf and nuc gene-specific PCR and 16S rRNA gene sequence. This label-free, non-invasive on-plate colony screening technology can be adopted by the food industries, biotechnology companies, and public health laboratories for Staphylococcus species detection including S. aureus from various samples for food safety and public health management. Graphical abstract.
Assuntos
Técnicas de Tipagem Bacteriana/métodos , Difusão Dinâmica da Luz/métodos , Contaminação de Alimentos/análise , Carne/análise , Leite/microbiologia , Staphylococcus/isolamento & purificação , Animais , Técnicas de Tipagem Bacteriana/economia , Bovinos , Galinhas/microbiologia , Difusão Dinâmica da Luz/economia , Microbiologia de Alimentos , Análise de Perigos e Pontos Críticos de Controle/métodos , RNA Ribossômico 16S/análise , RNA Ribossômico 16S/genética , Infecções Estafilocócicas/microbiologia , Staphylococcus/genética , Staphylococcus/crescimento & desenvolvimentoRESUMO
Food authenticity and food safety are of high importance to organizations as well as to the food industry to ensure an accurate labeling of food products. Respective analytical methods should provide a fast screening and a reliable cost-efficient quantitation. HPTLC was pointed out as key analytical technique in this field. A new HPTLC method applying caffeine-impregnated silica gel plates was developed for eight most frequently found fat-soluble azo dyes unauthorizedly added to spices, spice mixtures, pastes, sauces, and palm oils. A simple post-chromatographic UV irradiation provided an effective sample cleanup, which took 4 min for up to 46 samples in parallel. The method was trimmed to enable 23 simultaneous separations within 20 min for quantitation or 46 separations within 5 min for screening. Linear (4-40 ng/band) or polynomial (10-200 ng/band) calibrations of the eight azo dyes revealed high correlation coefficients and low standard deviations. Limits of detection and quantification were determined to be 2-3 and 6-9 ng/zone, respectively. After an easy sample extraction, recoveries of 70-120% were obtained from chili, paprika, and curcuma powder as well as from chili sauce, curry paste, and palm oil spiked at low (mainly 25-50 mg/kg) and high levels (150-300 mg/kg). For unequivocal identification, the compound in a suspect zone was eluted via a column into the mass spectrometer. This resulted in the hyphenation HPTLC-vis-HPLC-DAD-ESI-MS. Graphical abstract Simplified clean-up by UV irradiation for Sudan dye analysis in food by HPTLC-vis-HPLC-DAD-ESI-MS.
Assuntos
Compostos Azo/análise , Cromatografia em Camada Fina/métodos , Corantes de Alimentos/análise , Contaminação de Alimentos/análise , Análise de Perigos e Pontos Críticos de Controle/métodos , Naftóis/análise , Cromatografia Líquida de Alta Pressão/economia , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia em Camada Fina/economia , Limite de Detecção , Óleo de Palmeira/análise , Especiarias/análise , Fatores de TempoRESUMO
Sterigmatocystin (STG) is a highly toxic secondary fungal metabolite structurally closely related to the well-known carcinogenic aflatoxins. Its presence has been reported in grains and grain-based products as well as in other foodstuffs like nuts, green coffee beans, spices, beer and cheese. Due to the lack of suitable data on the occurrence of STG, in 2013, the European Food Safety Authority (EFSA) could not characterise its risk for human health and recommended that more data on STG in food and feed needed to be collected. In order to provide a new tool for the specific detection of STG, a competitive enzyme-linked immunosorbent assay (ELISA) was developed, optimised and validated in this study based on a sensitive monoclonal antibody specific to STG with no cross-reactivity with aflatoxins. The sample preparation method for rice, wheat and maize was based on a modified QuEChERS (quick, easy, cheap, effective, rugged and safe) approach. The assay was validated for the detection of STG in rice, wheat and maize in accordance with the guidelines for validation of semi-quantitative screening methods included in Commission Regulation (EU) 519/2014. The screening target concentration (STC) was set at 1.5 µg/kg. The cutoffs for rice, wheat and maize were 1.2, 1.2 and 1.3 µg/kg and the false suspected rates were 0.34, 1.15 and 0.78%, respectively. Good correlation was found between the results obtained by the STG ELISA and LC-MS/MS method for naturally contaminated rice samples. This validated method can be applied as a sensitive and high-throughput screening for the presence of STG in a range of agricultural commodities. Graphical abstract A new enzyme-linked immunosorbent assay based on an antibody specific to sterigmatocystin for the detection of this mycotoxin in corn, wheat and rice.
Assuntos
Ensaio de Imunoadsorção Enzimática/métodos , Análise de Perigos e Pontos Críticos de Controle/métodos , Esterigmatocistina/análise , Animais , Anticorpos Monoclonais/química , Ensaio de Imunoadsorção Enzimática/economia , Limite de Detecção , Camundongos , Oryza/química , Fatores de Tempo , Triticum/química , Zea mays/químicaRESUMO
Nowadays, the control of pesticide residues in food is well established. The capacity of triple quadrupole technology to satisfy the current food regulations has been demonstrated. However, the permanent high demand of consumers for more sensitive and faster testing is driving the development of improved analytical methodologies that increase the performances of sensitivity and robustness and reduce the analysis time. In this work, the feasibility of decreasing the run time to 12.4 min by modifying the oven temperature program, for a multiresidue method covering 203 pesticides, was evaluated. Satisfactory sensitivity results were achieved by reaching a limit of quantitation of 2 µg kg-1 for a great variety of fruits and vegetables. The validated method based on updated GC-QqQ-MS/MS has confirmed the abovementioned challenges with adequate robustness by its application to routine analyses for 69 real samples. The proposed method can represent great benefit for laboratories as it allows increasing samples throughput. It is also very useful for risk assessment studies, where the needs of low reporting limits and very wide analytical scope are necessary.