RESUMO
Anticoagulant rodenticides have been widely used to eliminate wild rodents, which as invasive species on remote islands can disturb ecosystems. Since rodenticides can cause wildlife poisoning, it is necessary to evaluate the sensitivity of local mammals and birds to the poisons to ensure the rodenticides are used effectively. The Bonin Islands are an archipelago located 1000 km southeast of the Japanese mainland and are famous for the unique ecosystems. Here the first-generation anticoagulant rodenticide diphacinone has been used against introduced black rats (Rattus rattus). The only land mammal native to the archipelago is the Bonin fruit bat (Pteropus pselaphon), but little is known regarding its sensitivity to rodenticides. In this study, the Egyptian fruit bats (Rousettus aegyptiacus) was used as a model animal for in vivo pharmacokinetics and pharmacodynamics analysis and in vitro enzyme kinetics using their hepatic microsomal fractions. The structure of vitamin K epoxide reductase (VKORC1), the target protein of the rodenticide in the Bonin fruit bat, was predicted from its genome and its binding affinity to rodenticides was evaluated. The Egyptian fruit bats excreted diphacinone slowly and showed similar sensitivity to rats. In contrast, they excreted warfarin, another first-generation rodenticide, faster than rats and recovered from the toxic effect faster. An in silico binding study also indicated that the VKORC1 of fruit bats is relatively tolerant to warfarin, but binds strongly to diphacinone. These results suggest that even chemicals with the same mode of action display different sensitivities in different species: fruit bat species are relatively resistant to warfarin, but vulnerable to diphacinone.
Assuntos
Quirópteros , Rodenticidas , Animais , Anticoagulantes/toxicidade , Quirópteros/metabolismo , Ecossistema , Mamíferos/metabolismo , Fenindiona/análogos & derivados , Ratos , Rodenticidas/toxicidade , Toxicocinética , Vitamina K Epóxido Redutases/genética , Vitamina K Epóxido Redutases/metabolismo , Varfarina/toxicidadeRESUMO
Anticoagulant rodenticides (ARs) are used worldwide for the control of rodent pests and are the main method of control of rat pest populations in agricultural areas. The main aim of this review is to discuss the risk of ARs to non-target wildlife in oil palm areas in Southeast Asia, mainly Indonesia and Malaysia. We discussed AR use in oil palm areas and toxicities of ARs on target and non-target animals. We also reviewed published literature on wildlife species reported in oil palm areas in Southeast Asia and utilizing this information, we assessed the hazard risk of ARs to non-target wildlife in oil palm plantations. ARs are a secondary exposure hazard to rodent-consuming mammalian carnivores, such as leopard cats and civets, and rodent-consuming raptors, such as barn owls. Consumption of dead poisoned prey puts scavengers, such as water monitors, at high risk for AR exposure. Domestic livestock and granivorous birds are at high risk for AR exposure via primary exposure to toxic bait, while omnivores such as macaques and wild pigs are at moderate risk for both primary and secondary exposure to ARs. The effects of ARs on barn owls have been well studied in the field and in laboratory secondary toxicity studies. Thus, the nest-box occupancy and reproductive parameters of local barn owl populations can be monitored as an indicator of the AR exposure level in the area. CLINICAL TRIALS REGISTRATION: No clinical trials were involved in this study.
Assuntos
Aves Predatórias , Rodenticidas , Estrigiformes , Animais , Animais Selvagens , Anticoagulantes/toxicidade , Sudeste Asiático , Mamíferos , Ratos , Rodenticidas/toxicidadeRESUMO
Although anticoagulant rodenticides (ARs) are effectively used for the control of invasive rodents, nontarget species are also frequently exposed to ARs and secondary poisonings occur widely. However, little data is available on the effects of ARs, especially on marine organisms. To evaluate the effects of ARs on marine wildlife, we chose green sea turtles (Chelonia mydas), which are one of the most common marine organisms around the Ogasawara islands, as our primary study species. The sensitivity of these turtles to ARs was assessed using both in vivo and in vitro approaches. We administered 4 mg/kg of warfarin sodium either orally or intravenously to juvenile green sea turtles. The turtles exhibited slow pharmacokinetics, and prolongation of prothrombin time (PT) was observed only with intravenous warfarin administration. We also conducted an in vitro investigation using liver microsomes from green sea turtles, and two other turtle species (softshell turtle and red-eared slider) and rats. The cytochrome P450 metabolic activity in the liver of green sea turtles was lower than in rats. Additionally, vitamin K epoxide reductase (VKOR), which is the target enzyme of ARs, was inhibited by warfarin in the turtles at lower concentration levels than in rats. These data indicate that turtles may be more sensitive to ARs than rats. We expect that these findings will be helpful for sea turtle conservation following accidental AR-broadcast incidents.
Assuntos
Anticoagulantes/toxicidade , Erros Inatos do Metabolismo/sangue , Rodenticidas/toxicidade , Tartarugas/sangue , Poluentes Químicos da Água/toxicidade , Animais , Resistência a Medicamentos , Ilhas , Fígado/efeitos dos fármacos , Fígado/metabolismo , Tempo de Protrombina , Ratos , Medição de Risco , Tartarugas/metabolismoRESUMO
The present work deals with the extraction and purification of chondroitin sulfate/dermatan sulfate from skin (CSG) and bone (CBG) of corb (Sciaena umbra). Electrophoresis of these polymers in barium acetate buffer on cellulose acetate revealed two fractions similar to dermatan sulfate and chondroitin sulfate. The in vivo anticoagulant activity of both chondroitin sulfate/dermatan sulfate (CS/DS) were evaluated, at 25 and 75 mg kg-1 of body weight (b.w), using activated partial thromboplastin time (aPTT), prothrombine time (TT) and thrombin time (PT) tests. Results showed that aPTT of CSG and CBG at 75 mg kg-1 of b.w were prolonged by 1.59 and 1.48-fold respectively, compared with the control. Further, toxicity studies on liver performed by the catalytic activity of transaminases in plasma, oxidative stress markers and hepatic morphological changes demonstrated that CSG and CBG at both doses are not toxics. In summary, the higher activity and lower toxicity of both CS/DS, especially at 25 mg kg-1 of b.w, recommended these compounds as a better drug candidate.
Assuntos
Anticoagulantes/farmacologia , Sulfatos de Condroitina/farmacologia , Dermatan Sulfato/farmacologia , Peixes/metabolismo , Animais , Anticoagulantes/isolamento & purificação , Anticoagulantes/toxicidade , Testes de Coagulação Sanguínea , Osso e Ossos/química , Varredura Diferencial de Calorimetria , Sulfatos de Condroitina/isolamento & purificação , Sulfatos de Condroitina/toxicidade , Dermatan Sulfato/isolamento & purificação , Dermatan Sulfato/toxicidade , Avaliação Pré-Clínica de Medicamentos , Eletroforese em Acetato de Celulose , Feminino , Glicosaminoglicanos/isolamento & purificação , Fígado/efeitos dos fármacos , Testes de Função Hepática , Microscopia Eletrônica de Varredura , Estresse Oxidativo/efeitos dos fármacos , Ratos Wistar , Pele/química , Difração de Raios XRESUMO
Degradation kinetics of oral anticoagulant rivaroxaban (RIV) was assessed in acid and alkaline media and while exposed to UVC radiation. Among all stress conditions tested, kinetic degradation process was better described by a zero-order model. A stability indicating method was validated for the analysis of the anticoagulant RIV in tablets by high-performance liquid chromatography. Robustness was evaluated with a two-level Plackett-Burman experimental design. The effect of acute exposition of the human hepatoblastoma HepG2 cell line to RIV stressed samples (100 and 500 µM) was assessed through in vitro toxicity tests. MTT reduction, neutral red uptake, mitochondrial membrane potential, and low molecular weight DNA diffusion assays were employed for cytotoxicity evaluation (5×104 cells/well). The genotoxic potential was assessed by comet assay (2×104 cells/well). Acute toxicity to HepG2 cells was assessed after 24 h incubation with sample solutions, for each test. A direct relationship between the increased amount of alkaline degradation products and higher cytotoxic potential was found. Results obtained by viability assay investigations support the concerns on risks associated with acute toxicity and genotoxicity of pharmaceutical samples containing degradation products as impurities.
Assuntos
Anticoagulantes/toxicidade , Dano ao DNA , Rivaroxabana/toxicidade , Anticoagulantes/efeitos da radiação , Técnicas de Cultura de Células , Ensaio Cometa , Estabilidade de Medicamentos , Células Hep G2 , Humanos , Hidrólise , Cinética , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Rivaroxabana/efeitos da radiação , Testes de ToxicidadeRESUMO
Based on detection of hepatic residues, scavenging and predatory non-target raptors are widely exposed to second generation anticoagulant rodenticides (SGARs). A small proportion, generally <10%, of tested birds are diagnosed as acutely poisoned. Little is known, however, of sub-lethal effects of SGARs, such as interaction of clotting capacity with traumatic injury. Assessment of coagulation function of birds submitted live to wildlife rehabilitators or veterinarians may provide a means of establishing the proportion of animals suffering sub-lethal coagulopathies, as well as identifying individuals requiring treatment. As a first step in exploring the potential of this approach, we dosed Japanese quail (Coturnix japonica) with the SGAR, brodifacoum, at 0, 0.8, 1.4, 1.9, and 2.5 mg/kg and sampled birds at 1, 3, 5 and 7 days post-dosing. Prothrombin time (PT), which measures the extrinsic coagulation pathway, was significantly prolonged in 98% of brodifacoum-exposed quail in a dose- and time-dependent manner. 50-fold prolongation of PT occurred at higher brodifacoum dosages and correlated to hemorrhage found at necropsy. Activated clotting time (ACT), a measure of the intrinsic pathway also increased with dose and time. Hemoglobin (Hb) and hematocrit (Hct) decreased dose- and time-dependently at doses ≥1.4 mg/kg with no significant change at 0.8 mg/kg. Reference intervals for PT (10.0-16.2 s), ACT (30-180 s), Hb (9.6-18.4 g/dl), and Hct (34-55%) were established in Japanese quail. Species-specific reference intervals are required as barn owl PT (17-29 s) and quail PT were different. The proportion of brodifacoum-exposed quail with hemorrhage was not correlated with liver residues, but was correlated with PT, suggesting that this assay is a useful indicator of avian anticoagulant rodenticide exposure. PTs measured in free-living barn owls sampled between April 2009 and August 2010 in the lower Fraser Valley of BC do not suggest significant exposure to SGARs.
Assuntos
4-Hidroxicumarinas/toxicidade , Anticoagulantes/toxicidade , Hemorragia/induzido quimicamente , Rodenticidas/toxicidade , 4-Hidroxicumarinas/administração & dosagem , Animais , Anticoagulantes/administração & dosagem , Coagulação Sanguínea/efeitos dos fármacos , Coturnix/metabolismo , Relação Dose-Resposta a Droga , Hemorragia/epidemiologia , Fígado/metabolismo , Tempo de Protrombina , Rodenticidas/administração & dosagem , Estrigiformes/metabolismo , Fatores de TempoRESUMO
In this study, biochemical and pharmacological characterization of Brevithrombolase, a fibrinolytic serine protease purified from Brevibacillus brevis strain FF02B has been reported. An assessment of its thrombolytic potency has also been made. The molecular mass of this monomeric protease was determined as 55 kDa, and 56043 Da, respectively, by SDS-PAGE and MALDI-TOF-MS. In the analytical studies, the N-terminal sequence of Brevithrombolase was found to be blocked; however, the peptide mass fingerprinting and amino acid composition analyses demonstrated the similarity of Brevithrombolase with endopeptidases in possessing serine in their catalytic triad. This finding was confirmed by the observation that the serine protease inhibitors decrease the catalytic (fibrinolytic) activity of Brevithrombolase. The secondary structure of Brevithrombolase was composed of 30.6% alpha helix and 69.4% random coil. Brevithrombolase showed the Km and Vmax values towards the chromogenic substrate for plasmin at 0.39 mM and 14.3 µmol/min, respectively. Brevithrombolase demonstrated optimum fibrinolytic activity at pH 7.4 and 37 °C, and showed marginal hydrolytic activity towards globulin, casein and fibrinogen. The anticoagulant potency of Brevithrombolase was comparable to the low molecular mass heparin/antithrombin-III and warfarin. Among the three enzymes-Brevithrombolase, plasmin and streptokinase-the fibrinolytic activity and in vitro thrombolytic potency of Brevithrombolase was found to be superior. The RP-HPLC and SDS-PAGE analyses suggested a similar pattern of fibrin degradation by Brevithrombolase and plasmin, indicating that former enzyme is a plasmin-like fibrinolytic serine protease. Brevithrombolase did not show in vitro cytotoxicity on HT29 and HeLa cells or hemolytic activity. At a dose of 10 mg/kg, Brevithrombolase did not exhibit lethality or toxicity on Wistar strain albino rats. Brevithrombolase did not inhibit factor Xa, and its mechanism of anticoagulant action was associated with the enzymatic cleavage of thrombin. The combined properties of Brevithrombolase indicate its therapeutic potential in peptide-based cardiovascular drug development.
Assuntos
Anticoagulantes/isolamento & purificação , Anticoagulantes/farmacologia , Brevibacillus/enzimologia , Fibrinólise/efeitos dos fármacos , Serina Proteases/isolamento & purificação , Serina Proteases/farmacologia , Amidas/metabolismo , Sequência de Aminoácidos , Animais , Anticoagulantes/química , Anticoagulantes/toxicidade , Bovinos , Coagulação Intravascular Disseminada/tratamento farmacológico , Coagulação Intravascular Disseminada/prevenção & controle , Fator Xa/metabolismo , Células HT29 , Células HeLa , Humanos , Dados de Sequência Molecular , Estrutura Secundária de Proteína , Ratos , Análise de Sequência , Serina Proteases/química , Serina Proteases/toxicidade , Trombina/antagonistas & inibidoresRESUMO
Philodryas baroni--an attractively colored snake--has become readily available through the exotic pet trade. Most people consider this species harmless; however, it has already caused human envenomation. As little is known about the venom from this South American opisthoglyphous "colubrid" snake, herein, we studied its protein composition by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), as well as its effects on the hemostatic system. Both reducing and nonreducing SDS-PAGE analysis demonstrated that the venom exhibits greatest complexity in the range of 50-80 kDa. The venom displayed proteolytic activity toward azocollagen, with a specific activity of 75.5 U mg⻹, and rapidly hydrolyzed the Aα-chain of fibrinogen, exhibiting lower activity toward the Bß- and γ-chains. The venom from P. baroni showed no platelet proaggregating activity per se, but it inhibited collagen- and thrombin-induced platelet aggregation. Prominent hemorrhage developed in mouse skin after intradermal injection of the crude venom, and its minimum hemorrhagic dose was 13.9 µg. When injected intramuscularly into the gastrocnemius of mice, the venom induced local effects such as hemorrhage, myonecrosis, edema, and leucocyte infiltration. Due to its venom toxicity shown herein, P. baroni should be considered dangerous to humans and any medically significant bite should be promptly reviewed by a qualified health professional.
Assuntos
Anticoagulantes/toxicidade , Colubridae , Endopeptidases/toxicidade , Inibidores da Agregação Plaquetária/toxicidade , Proteínas de Répteis/toxicidade , Venenos de Serpentes/toxicidade , Animais , Anticoagulantes/administração & dosagem , Anticoagulantes/química , Anticoagulantes/metabolismo , Argentina , Colágeno/metabolismo , Relação Dose-Resposta a Droga , Endopeptidases/administração & dosagem , Endopeptidases/química , Endopeptidases/metabolismo , Fibrinogênio/metabolismo , Hemolíticos/administração & dosagem , Hemolíticos/química , Hemolíticos/metabolismo , Hemolíticos/toxicidade , Hemorragia/induzido quimicamente , Humanos , Injeções Intradérmicas , Camundongos , Camundongos Endogâmicos , Peso Molecular , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/patologia , Necrose , Inibidores da Agregação Plaquetária/administração & dosagem , Inibidores da Agregação Plaquetária/química , Inibidores da Agregação Plaquetária/metabolismo , Proteínas de Répteis/administração & dosagem , Proteínas de Répteis/química , Proteínas de Répteis/metabolismo , Medição de Risco , Venenos de Serpentes/administração & dosagem , Venenos de Serpentes/química , Venenos de Serpentes/metabolismo , Especificidade por SubstratoRESUMO
In the United States, new regulatory restrictions have been placed on the use of some second-generation anticoagulant rodenticides. This action may be offset by expanded use of first-generation compounds (e.g., diphacinone; DPN). Single-day acute oral exposure of adult Eastern screech-owls (Megascops asio) to DPN evoked overt signs of intoxication, coagulopathy, histopathological lesions (e.g., hemorrhage, hepatocellular vacuolation), and/or lethality at doses as low as 130 mg/kg body weight, although there was no dose-response relation. However, this single-day exposure protocol does not mimic the multiple-day field exposures required to cause mortality in rodent pest species and non-target birds and mammals. In 7-day feeding trials, similar toxic effects were observed in owls fed diets containing 2.15, 9.55 or 22.6 ppm DPN, but at a small fraction (<5%) of the acute oral dose. In the dietary trial, the average lowest-observed-adverse-effect-level for prolonged clotting time was 1.68 mg DPN/kg owl/week (0.24 mg/kg owl/day; 0.049 mg/owl/day) and the lowest lethal dose was 5.75 mg DPN/kg owl/week (0.82 mg/kg owl/day). In this feeding trial, DPN concentration in liver ranged from 0.473 to 2.21 µg/g wet weight, and was directly related to the daily and cumulative dose consumed by each owl. A probabilistic risk assessment indicated that daily exposure to as little as 3-5 g of liver from DPN-poisoned rodents for 7 days could result in prolonged clotting time in the endangered Hawaiian short-eared owl (Asio flammeus sandwichensis) and Hawaiian hawk (Buteo solitarius), and daily exposure to greater quantities (9-13 g of liver) could result in low-level mortality. These findings can assist natural resource managers in weighing the costs and benefits of anticoagulant rodenticide use in pest control and eradication programs.
Assuntos
Anticoagulantes/toxicidade , Fenindiona/análogos & derivados , Rodenticidas/toxicidade , Estrigiformes/fisiologia , Administração Oral , Animais , Anticoagulantes/farmacocinética , Doença Hepática Induzida por Substâncias e Drogas/etiologia , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/patologia , Análise Custo-Benefício , Feminino , Hemorragia/induzido quimicamente , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Longevidade/efeitos dos fármacos , Masculino , Fenindiona/farmacocinética , Fenindiona/toxicidade , Rodenticidas/farmacocinética , Especificidade da Espécie , Testes de Toxicidade , Tempo de Coagulação do Sangue TotalRESUMO
OBJECTIVE: To assess the rate and extent of ruminal degradation of warfarin, chlorophacinone, and bromadiolone in vitro and determine the oral availability and clinical and hemostatic effects of each anticoagulant rodenticide in adult sheep. ANIMALS: 3 Texel sheep. PROCEDURE: Samples of ruminal fluid were incubated with each of the anticoagulants to assess the kinetics of ruminal degradation over 24 hours. To determine the plasma kinetics of the anticoagulants, each sheep received each of the anticoagulants IV or via a rumenimplanted cannula at 2-month intervals (3 rodenticide exposures/sheep). At intervals during a 240- to 360- hour period after treatment, prothrombin time (PT) was measured, plasma anticoagulant concentration was assessed, and clinical signs of rodenticide poisoning were monitored. In plasma and rumen extracts, anticoagulant concentrations were determined via high-performance liquid chromatography. RESULTS: In the rumen extracts, anticoagulants were slightly degraded (< 15%) over 24 hours. In vivo, oral availability of warfarin, chlorophacinone, and bromadiolone was estimated at 79%, 92%, and 88%, respectively. Although maximum PT was 80 seconds after chlorophacinone and bromadiolone treatments, no clinical signs of toxicosis were detected; PT returned to baseline values within 2 weeks. CONCLUSIONS AND CLINICAL RELEVANCE: In sheep, warfarin, chlorophacinone, and bromadiolone were not degraded in the rumen but their bioavailabilities were high after oral administration; the kinetics of these compounds in sheep and other mammals are quite similar. These data suggest that the lack of susceptibility of ruminants to these anticoagulant rodenticides cannot be explained by either ruminal degradation or the specific toxicokinetics of these anticoagulants.
Assuntos
Anticoagulantes/farmacocinética , Anticoagulantes/toxicidade , Rodenticidas/farmacocinética , Rodenticidas/toxicidade , Rúmen/metabolismo , Doenças dos Ovinos/induzido quimicamente , Ovinos/metabolismo , 4-Hidroxicumarinas/sangue , 4-Hidroxicumarinas/farmacocinética , 4-Hidroxicumarinas/toxicidade , Animais , Anticoagulantes/sangue , Disponibilidade Biológica , Indanos/sangue , Indanos/farmacocinética , Indanos/toxicidade , Masculino , Rodenticidas/sangue , Varfarina/sangue , Varfarina/farmacocinética , Varfarina/toxicidadeRESUMO
Coumarin (1,2-benzopyrone) is a naturally occurring fragrant compound found in a variety of plants and spices. Exposure to the general public is through the diet and from its use as a perfume raw material in personal care products. High doses of coumarin by the oral route are known to be associated with liver toxicity in rodents. Chronic oral bioassays conducted in the 1990s reported liver tumors in rats and mice and lung tumors in mice, raising concerns regarding the safety of coumarin. Since then, an extensive body of research has focused on understanding the etiology of these tumors. The data support a conclusion that coumarin is not DNA-reactive and that the induction of tumors at high doses in rodents is attributed to cytotoxicity and regenerative hyperplasia. The species-specific target organ toxicity is shown to be related to the pharmacokinetics of coumarin metabolism, with data showing rats to be particularly susceptible to liver effects and mice to be particularly susceptible to lung effects. A quantitative human health risk assessment that integrates both cancer and non-cancer effects is presented, confirming the safety of coumarin exposure from natural dietary sources as well as from its use as a perfume in personal care products.
Assuntos
Anticoagulantes/toxicidade , Cumarínicos/toxicidade , Animais , Cães , Exposição Ambiental , Humanos , Camundongos , Primatas , Ratos , Medição de Risco , Especificidade da Espécie , Testes de ToxicidadeRESUMO
Bivalirudin (Hirulog, Angiomax) is a specific, reversible and direct thrombin inhibitor with a predictable anticoagulant effect. It is cleared by both proteolytic cleavage and renal mechanisms, predominantly glomerular filtration. Bivalirudin inhibits both circulating thrombin and fibrin bound thrombin directly by binding to thrombin catalytic site and anion-binding exosite I in a concentration-dependent manner. Bivalirudin prolongs activated partial thromboplastin time, prothrombin time, thrombin time and activated clotting time (ACT). ACT levels with bivalirudin do not correlate with its clinical efficacy. Bivalirudin with a provisional GpIIb/IIIa inhibitor is indicated in elective contemporary percutaneous coronary intervention (PCI). In respect to combined ischemic and hemorrhagic endpoints of death, myocardial infarction, unplanned urgent revascularization and major bleeding during PCI (including subgroups of patients with renal impairment and diabetes) bivalirudin is not inferior to unfractioned heparin and planned GpIIb/IIIa inhibitors. In addition, bivalirudin has been consistently shown to have significantly less in-hospital major bleeding than heparin alone or heparin in combination with a GpIIb/IIIa inhibitor. Bivalirudin appears to be also safe and effective during PCI in patients with heparin-induced thrombocytopenia. Finally, data from PCI studies support the safety and efficacy of bivalirudin, although its direct randomized comparison with unfractionated heparin is lacking.
Assuntos
Anticoagulantes/farmacologia , Anticoagulantes/toxicidade , Anticoagulantes/uso terapêutico , Hirudinas/farmacologia , Hirudinas/toxicidade , Fragmentos de Peptídeos/farmacologia , Fragmentos de Peptídeos/toxicidade , Fragmentos de Peptídeos/uso terapêutico , Angioplastia Coronária com Balão , Animais , Anticoagulantes/química , Anticoagulantes/farmacocinética , Ponte Cardiopulmonar , Doença das Coronárias/tratamento farmacológico , Análise Custo-Benefício , Hirudinas/química , Hirudinas/farmacocinética , Humanos , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/farmacocinética , Complicações Pós-Operatórias/sangue , Complicações Pós-Operatórias/tratamento farmacológico , Proteínas Recombinantes/química , Proteínas Recombinantes/farmacocinética , Proteínas Recombinantes/farmacologia , Proteínas Recombinantes/uso terapêutico , Proteínas Recombinantes/toxicidade , Trombina/antagonistas & inibidoresRESUMO
The synthesis of condensation products of 4-hydroxycoumarin and nitro-substituted aromatic aldehydes as well as oximes of drugs with anticoagulant activity is described. The acute toxicity of the compounds was studied in mice by oral and intraperitoneal administration. A comparative pharmacological study of the in vivo anticoagulant effects of Warfarin derivatives showed that the new compounds have different anticoagulant activity. 4-Hydroxy-3-[1- (4-chlorophenyl)-3-oxobutyl]-2H-1-benzopyran-2-one, oxime 3 showed anticoagulant effect similar to Warfarin and Coumachlor, but its acute toxicity was higher than that of the reference drugs.