Biological-based techniques for real-time water-quality studies: Assessment of non-invasive (swimming consistency and respiration) and toxicity (antioxidants) biomarkers of zebrafish.

Swimming consistency and respiration of fish are recognized as the non-invasive stress biomarkers. Their alterations could directly indicate the presence of pollutants in the water ecosystem. Since these biomarkers are a routine process for fish, it is difficult to monitor their activity manually. For this reason, experts employ engineering technologies to create sensors that can monitor the regular activities of fish. Knowing the importance of these non-invasive stress biomarkers, we developed online biological behavior monitoring system-OBBMS and online biological respiratory response monitoring system-OBRRMS to monitor real-time swimming consistency and respiratory response of fish, respectively. We continuously monitored the swimming consistency and respiration (OCR, CER and RQ) of zebrafish (control and atrazine-treatments) for 7 days using our homemade real-time biological response monitoring systems. Furthermore, we analyzed oxidative stress indicators (SOD, CAT and POD) within the vital tissues (gills, brain and muscle) of zebrafish during stipulated sampling periods. The differences in the swimming consistency and respiratory rate of zebrafish between the control and atrazine treatments could be precisely differentiated on the real-time datasets of OBBMS and OBRRMS. The zebrafish exposed to atrazine toxin showed a concentration-dependent effect (hypoactivity). The OCR and CER were increased in the atrazine treated zebrafish. Both Treatment I and II received a negative response for RQ. Atrazine toxicity let to a rise in the levels of SOD, CAT and POD in the vital tissues of zebrafish. The continuous acquisition of fish signals is achieved which is one of the main merits of our OBBMS and OBRRMS. Additionally, no special data processing was done, the real-time data sets were directly used on statistical tools and the differences between the factors (groups, photoperiods, exposure periods and their interactions) were identified precisely. Hence, our OBBMS and OBRRMS could be a promising tool for biological response-based real-time water quality monitoring studies.

Holistic Assessment Based On Hepatocyte Mitochondria: Lycopene Repairs Oxidized mtDNA to Alleviate Mitochondrial Stress Induced by Atrazine.

Atrazine (ATZ) is a highly persistent herbicide that harms organism health. Lycopene (LYC) is an antioxidant found in plants and fruits. The aim of this study is to investigate the mechanisms of atrazine-induced mitochondrial damage and lycopene antagonism in the liver. The mice were divided into seven groups by randomization: blank control (Con group), vehicle control (Vcon group), 5 mg/kg lycopene (LYC group), 50 mg/kg atrazine (ATZ1 group), ATZ1+LYC group, 200 mg/kg atrazine (ATZ2 group), and ATZ2+LYC group. The present study performed a holistic assessment based on mitochondria to show that ATZ causes the excessive fission of mitochondria and disrupts mitochondrial biogenesis. However, the LYC supplementation reverses these changes. ATZ causes increased mitophagy and exacerbates the production of oxidized mitochondrial DNA (Ox-mtDNA) and mitochondrial stress. This study reveals that LYC could act as an antioxidant to repair Ox-mtDNA and restore the disordered mitochondrial function caused by ATZ.

Insight into Chinese medicine residue biochar combined with ultrasound for persulfate activation in atrazine degradation: Acanthopanax senticosus precursors, synergistic effects and toxicity assessment.

The synergistic activation of persulfate by multiple factors could degrade pollutants more efficiently. However, the co-activation method based on metal ions has the risk of leakage. The non-metallic coupling method could achieve the same efficiency as the metal activation and meanwhile release environmental stress. In this study, the original biochar (BC) was prepared through using Chinese medicinal residue of Acanthopanax senticosus as the precursor. Compared with other biochar, the pore size structure was higher and toxicity risk was lower. The ultrasonic (US)/Acanthopanax senticosus biochar (ASBC)/persulfate oxidation system was established for Atrazine (ATZ). Results showed that 45KHz in middle and low frequency band cooperated with ASBC600 to degrade nearly 70 % of ATZ within 50 min, and US promoted the formation of SO4- and OH. Meanwhile, the synergy index of US and ASBC was calculated to be 1.18, which showed positive synergistic effect. Finally, the potential toxicity was examined by using Toxicity Characteristic Leaching Procedure (TCLP) and luminescent bacteria. This study provides a promising way for the activation of persulfate, which is expected to bring a new idea for the win-win situation of pollutant degradation and solid waste resource utilization.

Health risk assessment of exposure to atrazine in the soil of Shiraz farmlands, Iran.

Atrazine-contaminated soils can pose a carcinogenic and non-carcinogenic health risk through different routes for exposed people. This study aimed to assess the health risk of exposure to atrazine-contaminated soils through direct ingestion and dermal contact in farmlands nearby Shiraz. Atrazine concentration was measured in 22 selected sites using grid sampling. The carcinogenic and non-cancer risks associated with dermal and ingestion exposure in children and adults were estimated. The lowest and highest atrazine concentrations were in S1 (0.015 mg/kg soil) and S22 (0.55 mg/kg soil). Hazard Index (HI)1 values ranged from 0.007 to 0.25 for children, and the values ranged from 0.0008 to 0.03 for adults. The mean cancer risk for children and adults was 6.01 × 10-4 and 7.40 × 10-5, respectively. The HI value was less than 1 for all sampling sites, indicating that exposure to atrazine does not threaten children and adults. However, the cancer risk exceeds the United States Environmental Protection Agency (US.EPA)2 threshold risk limit (10-6 to 10-4) in all sampling sites. Therefore, it is recommended that children should avoid playing on atrazine-contaminated farms or soils near anywhere atrazine may have been used.

Assessment of risks to listed species from the use of atrazine in the USA: a perspective.

Atrazine is a triazine herbicide used predominantly on corn, sorghum, and sugarcane in the US. Its use potentially overlaps with the ranges of listed (threatened and endangered) species. In response to registration review in the context of the Endangered Species Act, we evaluated potential direct and indirect impacts of atrazine on listed species and designated critical habitats. Atrazine has been widely studied, extensive environmental monitoring and toxicity data sets are available, and the spatial and temporal uses on major crops are well characterized. Ranges of listed species are less well-defined, resulting in overly conservative designations of "May Effect". Preferences for habitat and food sources serve to limit exposure among many listed animal species and animals are relatively insensitive. Atrazine does not bioaccumulate, further diminishing exposures among consumers and predators. Because of incomplete exposure pathways, many species can be eliminated from consideration for direct effects. It is toxic to plants, but even sensitive plants tolerate episodic exposures, such as those occurring in flowing waters. Empirical data from long-term monitoring programs and realistic field data on off-target deposition of drift indicate that many other listed species can be removed from consideration because exposures are below conservative toxicity thresholds for direct and indirect effects. Combined with recent mitigation actions by the registrant, this review serves to refine and focus forthcoming listed species assessment efforts for atrazine.Abbreviations: a.i. = Active ingredient (of a pesticide product). AEMP = Atrazine Ecological Monitoring Program. AIMS = Avian Incident Monitoring SystemArach. = Arachnid (spiders and mites). AUC = Area Under the Curve. BE = Biological Evaluation (of potential effects on listed species). BO = Biological Opinion (conclusion of the consultation between USEPA and the Services with respect to potential effects in listed species). CASM = Comprehensive Aquatic System Model. CDL = Crop Data LayerCN = field Curve Number. CRP = Conservation Reserve Program (lands). CTA = Conditioned Taste Avoidance. DAC = Diaminochlorotriazine (a metabolite of atrazine, also known by the acronym DACT). DER = Data Evaluation Record. EC25 = Concentration causing a specified effect in 25% of the tested organisms. EC50 = Concentration causing a specified effect in 50% of the tested organisms. EC50RGR = Concentration causing a 50% reduction in relative growth rate. ECOS = Environmental Conservation Online System. EDD = Estimated Daily Dose. EEC = Expected Environmental Concentration. EFED = Environmental Fate and Effects Division (of the USEPA). EFSA = European Food Safety Agency. EIIS = Ecological Incident Information System. ERA = Environmental Risk Assessment. ESA = Endangered Species Act. ESU = Evolutionarily Significant UnitsFAR = Field Application RateFIFRA = Federal Insecticide, Fungicide, and Rodenticide Act. FOIA = Freedom of Information Act (request). GSD = Genus Sensitivity Distribution. HC5 = Hazardous Concentration for ≤ 5% of species. HUC = Hydrologic Unit Code. IBM = Individual-Based Model. IDS = Incident Data System. KOC = Partition coefficient between water and organic matter in soil or sediment. KOW = Octanol-Water partition coefficient. LC50 = Concentration lethal to 50% of the tested organisms. LC-MS-MS = Liquid Chromatograph with Tandem Mass Spectrometry. LD50 = Dose lethal to 50% of the tested organisms. LAA = Likely to Adversely Affect. LOAEC = Lowest-Observed-Adverse-Effect Concentration. LOC = Level of Concern. MA = May Affect. MATC = Maximum Acceptable Toxicant Concentration. NAS = National Academy of Sciences. NCWQR = National Center of Water Quality Research. NE = No Effect. NLAA = Not Likely to Adversely Affect. NMFS = National Marine Fisheries Service. NOAA = National Oceanic and Atmospheric Administration. NOAEC = No-Observed-Adverse-Effect Concentration. NOAEL = No-Observed-Adverse-Effect Dose-Level. OECD = Organization of Economic Cooperation and Development. PNSP = Pesticide National Synthesis Project. PQ = Plastoquinone. PRZM = Pesticide Root Zone Model. PWC = Pesticide in Water Calculator. QWoE = Quantitative Weight of Evidence. RGR = Relative growth rate (of plants). RQ = Risk Quotient. RUD = Residue Unit Doses. SAP = Science Advisory Panel (of the USEPA). SGR = Specific Growth Rate. SI = Supplemental Information. SSD = Species Sensitivity Distribution. SURLAG = Surface Runoff Lag Coefficient. SWAT = Soil & Water Assessment Tool. SWCC = Surface Water Concentration Calculator. UDL = Use Data Layer (for pesticides). USDA = United States Department of Agriculture. USEPA = United States Environmental Protection Agency. USFWS = United States Fish and Wildlife Service. USGS = United States Geological Survey. WARP = Watershed Regressions for Pesticides.

Assessment of the risk of death of Clarias gariepinus and Oreochromis niloticus pulse-exposed to selected agricultural pesticides.

Aquatic organisms are often exposed briefly to high pesticide concentration. Survival time model was used to study risk of death in C. gariepinus and O. niloticus fingerlings exposed to 24 mg/L atrazine, 42 mg/l mancozeb, 1 mg/L chlorpyrifos and 0.75 µg/L lambda cyhalothrin for 15, 30, 45 and 60 minutes and continuously for 96 hours. Mortality, time-to-death, weight, length, and condition factor of the fingerlings were recorded. Results obtained showed tilapia was more susceptible than catfish to continuous exposure but not pulse exposure. The survival probability of both species was similar when exposed for 15, 30 and 45 minutes (p > 0.05) but differed after 60 minutes (p < 0.05). Risk of death of catfish exposed briefly to atrazine, mancozeb and chlorpyrifos for 60 minutes was similar to 96 hours continuous exposure, same for tilapia exposed to 1 mg/L chlorpyrifos (p > 0.05). Survival probability of tilapia exposed to chlorpyrifos for 15, 30, 45 and 60 minutes was similar (p > 0.05) and was not influenced by pulse length. Pesticide hazard and risk of death decreased as fish size (weight, length, and condition factor) increased. Pulse toxicity assessment using survival models could make pesticides exposure assessment more realistic by studying factors that can influence the toxicity of pesticides.

Low-Cost Chlorophyll Fluorescence Imaging for Stress Detection.

Plants naturally contain high levels of the stress-responsive fluorophore chlorophyll. Chlorophyll fluorescence imaging (CFI) is a powerful tool to measure photosynthetic efficiency in plants and provides the ability to detect damage from a range of biotic and abiotic stresses before visible symptoms occur. However, most CFI systems are complex, expensive systems that use pulse amplitude modulation (PAM) fluorometry. Here, we test a simple CFI system, that does not require PAM fluorometry, but instead simply images fluorescence emitted by plants. We used this technique to visualize stress induced by the photosystem II-inhibitory herbicide atrazine. After applying atrazine as a soil drench, CFI and color images were taken at 15-minute intervals, alongside measurements from a PAM fluorometer and a leaf reflectometer. Pixel intensity of the CFI images was negatively correlated with the quantum yield of photosystem II (ΦPSII) (p < 0.0001) and positively correlated with the measured reflectance in the spectral region of chlorophyll fluorescence emissions (p < 0.0001). A fluorescence-based stress index was developed using the reflectometer measurements based on wavelengths with the highest (741.2 nm) and lowest variability (548.9 nm) in response to atrazine damage. This index was correlated with ΦPSII (p < 0.0001). Low-cost CFI imaging can detect herbicide-induced stress (and likely other stressors) before there is visual damage.

Risk assessment and acute toxicological effects of atrazine on Nile tilapia larvae after stress.

The present study aimed to evaluate the risk assessment, median lethal concentration LC50-96h, development, and mortality of Nile tilapia Oreochromis niloticus larvae exposed to atrazine after stress management. An LC50-96h trial was carried out using fish (n = 147; 8.5 ± 1.0 mg; seven larvae/aquarium), which were allocated randomly in 21 aquaria (1 L) and exposed to five concentrations of atrazine: 2, 4, 8, 16, and 32 mg L-1 plus one control (without herbicide) and a control with a solubility adjuvant (acetone). Temperature, pH, dissolved oxygen, conductivity, and total ammonia were measured daily. In addition, a stress test was performed with fish (n = 150; 17.9 ± 1.7 mg; 10 larvae/aquarium) submitted to air exposition (five minutes) and posteriorly distributed randomly in 15 aquaria (1 L), and exposed to atrazine at 0.18, 6, 12, and 18 mg L-1. The LC50-96h of atrazine for Nile tilapia larvae was 17.87 mg L-1. Significant differences (P < 0.05) were found for weight and final length, pH, and dissolved oxygen that was responsive to the increased levels of herbicide. Larvae mortality post-stress was registered to 6, 12, and 18 mg L-1 of atrazine, and was more critical at 24 h. The increased doses of atrazine used in LC50-96h test depressed the development of Nile tilapia larvae. Fish submitted to stress (air exposition) and exposed to sub-lethal doses of atrazine showed significant mortality, indicating that stressors may increase the toxic effect of atrazine for Nile tilapia larvae. On the other hand, based on risk assessment atrazine can be classified as herbicide with low toxicity for Nile tilapia larvae and low toxicological risk.

Activation of peroxymonosulfate by cobalt-impregnated biochar for atrazine degradation: The pivotal roles of persistent free radicals and ecotoxicity assessment.

Cobalt-mediated activation of peroxymonosulfate (PMS) has been extensively investigated for the degradation of emerging organic pollutants. In this study, PMS activation via cobalt-impregnated biochar towards atrazine (ATZ) degradation was systematically examined, and the underlying reaction mechanism was explicated. It was found that persistent free radicals (PFRs) contained in biochar play a pivotal role in PMS activation process. The PFRs enabled an efficient transfer electron to both cobalt atom and O2, facilitating the recycle of Co(III)/Co(II), and thereby leaded to an excellent catalytic performance. In contrast to oxic condition, the elimination of dissolved oxygen significantly retarded the ATZ degradation efficiency from 0.76 to 0.36 min-1. Radical scavenging experiments and electron paramagnetic resonance (EPR) analysis confirmed that the ATZ degradation was primarily due to SO4·- and, to a lesser extent, ·OH. In addition, dual descriptor (DD) method was carried out to reveal reactive sites on ATZ for radicals attacking and predicted derivatives. Meanwhile, the possible ATZ degradation pathways were accordingly proposed, and the ecotoxicity evaluation of the oxidation intermediates was also conducted by ECOSAR. Consequently, the cobalt-impregnated biochar could be an efficient and environmentally friendly catalyst to activate PMS for abatement and detoxication of ATZ.

Assessment of the effects of atrazine, dichlorodiphenyltrichloroethane, and dimethoate on freshwater fish (Oreochromis mossambicus): a case study of the A2 farmlands in Chiredzi, in the southeastern part of Zimbabwe.

A study was carried out to assess the levels and effects of atrazine, dimethoate, and dichlorodiphenyltrichloroethane on freshwater fish (Oreochromis mossambicus). Water and fish were sampled once from a dam within the Hippo Valley A2 farmlands in Chiredzi, in the southeastern part of Zimbabwe. The samples were screened for atrazine, dimethoate, and dichlorodiphenyltrichloroethane (DDT), the pesticides commonly used in the region. Atrazine and dimethoate are pesticides commonly used in the control of weeds and pests in the agricultural production of sugarcane in Hippo Valley, Chiredzi region. The effects of the pesticides on biochemical endpoints of the sampled fish were determined. The analyzed biochemical end parameters were superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase activities in liver and white muscle fish extracts. Dichlorodiphenyltrichloroethane was observed in water and fish muscle tissue at concentrations of 131.3 µg/l and 171.7 µg/kg, respectively, while atrazine was detected at concentrations of 6.15 µg/l and 142.0 µg/kg in water and fish muscle tissue, respectively. The observed concentrations of atrazine and DDT in water samples were above the limits permissible by the World Health Organization in drinking water. Dimethoate was found in concentrations of 4.21 µg/l and 1.30 µg/kg in water and fish muscle tissue respectively. The observed concentration of dimethoate in water was below the acceptable limit set by the Guidelines for Canadian Drinking Water. Antioxidant enzyme activities were increased significantly (p < 0.05) in fish exposed to water from Hippo Valley, Chiredzi, when compared with the controls. The enhanced activities of the studied antioxidant enzyme system were attributed to exposure to pollutants in the water body. Alterations of the biochemical integrity of fish indicate negative effects of the pesticides on the well-being of fish and undoubtedly other aquatic biota as well.

Environmental risk assessment of triazine herbicides in the Bohai Sea and the Yellow Sea and their toxicity to phytoplankton at environmental concentrations.

Herbicides have been increasingly used worldwide and a large amount of herbicide residue eventually enters the ocean via groundwater or surface run-off every year. However, the global coastal pollution status of herbicides and their negative impact on marine life (especially phytoplankton) in natural environmental concentrations are poorly understood except for few special environments (e.g. the Great Barrier Reef, Australia). Our field investigation of the distribution of ten triazine herbicides in the Bohai Sea and the Yellow Sea of China revealed that the concentrations of triazine herbicides exceeded the "No Observed Effect Concentrations" for phytoplankton. Their total concentrations could be as high as 6.61 nmol L-1. Based on the concentration addition model, the toxicity of herbicide homologues is usually cumulative, and the combined toxicity of these ten triazine herbicides could cause 13.2% inhibition on the chlorophyll a fluorescence intensity of a representative diatom species Phaeodactylum tricornutum Pt-1, which corresponds roughly to the toxicity of atrazine in an equivalent concentration of 14.08 nmol L-1. Atrazine in this equivalent-effect concentration could greatly inhibit the growth of cells, the maximum quantum efficiency of photosystem II (Fv/Fm), and nutrient absorption of Phaeodactylum tricornutum Pt-1. Transcriptome analysis revealed that multiple metabolic pathways (Calvin cycle, tricarboxylic acid (TCA) cycle, glycolysis/gluconeogenesis, etc.) related with photosynthesis and carbon metabolism were greatly disturbed, which might ultimately influence the primary productivity of coastal waters. Moreover, with the values of its bioaccumulation factor ranging from 69.6 to 118.9, atrazine was found to be accumulated in algal cells, which indicates that herbicide pollution might eventually affect the marine food web and even threaten the seafood safety of human beings.

Strength of methods assessment for aquatic primary producer toxicity data: A critical review of atrazine studies from the peer-reviewed literature.

Improving the quality of pesticide toxicity studies is a shared goal in ecotoxicology and a priority for risk assessors. Using the herbicide atrazine and testing on primary producers as a case study, we developed and applied a transparent scoring system for assessing the quality of peer-reviewed studies. The exercise also highlights where data gaps exist for planning future work. We determined that, while a large number of studies (147) present experimental data fitting basic inclusion criteria, only a small proportion provide sufficient information on the test substance, test organism, and test results to be considered of sufficient quality (i.e., a minimum score of >8 out of 16, meaning no critical study weaknesses identified) that would allow recommendation for their use in decision-making. Optimal studies for use in first tier risk assessment were further identified for each taxonomic group as the highest-scoring study scoring >8, that also used the technical grade active ingredient, reported an EC50 for a population-level endpoint (e.g. cell density, dry weight), and an exposure period in line with standard tests (≤96-h for algae, ≤14-d for macrophytes). Ultimately, 22 freshwater studies (four periphyton, ten macrophytes, and eight phytoplankton) achieved scores >8. Only one study with marine phytoplankton scored >8, and no studies met the risk assessment inclusion criteria for marine/estuarine periphyton or macrophytes. This indicates a potential research need with respect to toxicity data for salt-water species. Finally, registrant studies were evaluated, and in many cases, were the most appropriate for risk assessment, with the greatest scores observed for their respective species relative to those reported in the peer-reviewed literature. This exercise highlights the importance of defining and identifying well-performed toxicity tests, illuminating knowledge gaps, and reporting high quality data in support of the risk assessment process outside of the standard regulatory framework.

Adapting population models for application in pesticide risk assessment: A case study with Mead's milkweed.

Population models can facilitate assessment of potential impacts of pesticides on populations or species rather than individuals and have been identified as important tools for pesticide risk assessment of nontarget species including those listed under the Endangered Species Act. Few examples of population models developed for this specific purpose are available; however, population models are commonly used in conservation science as a tool to project the viability of populations and the long-term outcomes of management actions. We present a population model for Mead's milkweed (Asclepias meadii), a species listed as threatened under the Endangered Species Act throughout its range across the Midwestern United States. We adapted a published population model based on demographic field data for application in pesticide risk assessment. Exposure and effects were modeled as reductions of sets of vital rates in the transition matrices, simulating both lethal and sublethal effects of herbicides. Two herbicides, atrazine and mesotrione, were used as case study examples to evaluate a range of assumptions about potential exposure-effects relationships. In addition, we assessed buffers (i.e., setback distances of herbicide spray applications from the simulated habitat) as hypothetical mitigation scenarios and evaluated their influence on population-level effects in the model. The model results suggest that buffers can be effective at reducing risk from herbicide drift to plant populations. These case studies demonstrate that existing population models can be adopted and integrated with exposure and effects information for use in pesticide risk assessment. Environ Toxicol Chem 2018;37:2235-2245. © 2018 SETAC.

Assessment of atrazine toxicity to the estuarine phytoplankter, Dunaliella tertiolecta (Chlorophyta), under varying nutrient conditions.

Anthropogenic inputs of chemical environmental contaminants are frequently associated with developing harmful algal blooms, but little is known about how estuarine phytoplankton assemblages respond to multiple, co-occurring chemical stressors in chronically disturbed habitats. The goals of this research were to establish a robust protocol for testing the effects of atrazine on estuarine phytoplankton, and then to use that protocol to compare the effects of atrazine exposure with and without nutrient enrichment on a cosmopolitan estuarine/marine alga, Dunaliella tertiolecta (Chlorophyta). Atrazine sensitivity in nutrient-replete media (96-h growth inhibition [Formula: see text]) was 159.16 µg l-1, but sensitivity was influenced by exposure duration, and inhibitory effects of herbicide on algal growth decreased under imbalanced nutrient regimes and low nitrogen and phosphorus supplies. These findings advance knowledge about how nutrient regimes and herbicides interact to control estuarine phytoplankton population dynamics.

Assessment of bacterial biodetoxification of herbicide atrazine using Aliivibrio fischeri cytotoxicity assay with prolonged contact time.

In our study, we determined and compared the atrazine-biodetoxification ability of 41 bacterial strains and 21 consortia created of those with over 50% degradation rate in pure cultures. Biodegradation capacity was measured with GC-MS. Detoxification was assessed based on the cytotoxic effect of end-products to Aliivibrio fischeri in chronic bioluminescence inhibition assay with 25 h contact time. Chronic A. fischeri assay adapted to a microplate, which is suitable for examine numerous residues simultaneously, also appeared to be significantly more sensitive to atrazine compared to the standard acute (30 min) test. Due to its sensitivity, the chronic assay could be a valuable tool to provide a more comprehensive view of the ecological risks of atrazine and other chemicals. Thirteen strains were able to degrade more than 50% of 50 ppm atrazine. Four of these belong to Rhodococcus aetherivorans, R. qingshengii, Serratia fonticola and Olivibacter oleidegradans which species' atrazine degrading ability has never been reported before. Four consortia degrading ability was more effective than that of the creating individual strains; moreover, their residues did not show cytotoxic effects to A. fischeri. However, in several cases, the degradation products of sole strains and consortia resulted in significant bioluminescence inhibition. Thus high biodegradation (>90%) does not certainly mean the reduction or cessation of toxicity highlighting the importance of the evaluation of biological effects of degradation residues to improve the efficiency and abate the ecological risks of bioremediation techniques.

Fast algal eco-toxicity assessment: Influence of light intensity and exposure time on Chlorella vulgaris inhibition by atrazine and DCMU.

In order to develop a rapid assay suitable for algal eco-toxicity assessments under conditions representative of natural ecosystems, this study evaluated the short-term (<1h) response of algae exposed to atrazine and DCMU using oxygen productivity measurements. When Chlorella vulgaris was exposed to these herbicides under 'standard' low light intensity (as prescribed by OECD201 guideline), the 20min-EC50 values recorded via oxygen productivity (atrazine: 1.32±0.07µM; DCMU: 0.31±0.005µM) were similar the 96-h EC50 recorded via algal growth (atrazine: 0.56µM; DCMU: 0.41µM), and within the range of values reported in the literature. 20min-EC50 values increased by factors of 3.0 and 2.1 for atrazine and DCMU, respectively, when light intensity increased from 60 to 1400µmolm-2s-1 of photosynthetically active radiation, or PAR. Further investigation showed that exposure time significantly also impacted the sensitivity of C. vulgaris under high light intensity (>840µmolm-2s-1 as PAR) as the EC50 for atrazine and DCMU decreased by up to 6.2 and 2.1 folds, respectively, after 50min of exposure at a light irradiance of 1400µmolm-2s-1 as PAR. This decrease was particularly marked at high light intensities and low algae concentrations and is explained by the herbicide disruption of the electron transfer chain triggering photo-inhibition at high light intensities. Eco-toxicity assessments aiming to understand the potential impact of toxic compounds on natural ecosystems should therefore be performed over sufficient exposure times (>20min for C. vulgaris) and under light intensities relevant to these ecosystems.

Safety assessment of nanopesticides using the roundworm Caenorhabditis elegans.

The extensive use of pesticides is causing environmental pollution, affecting animal organisms in different habitats and also leading human health at risk. In this study, we present as an alternative the use of nanoparticles loaded with pesticides and report their toxicological assessment to a soil organism, Caenorhabditis elegans. Three nanoparticle formulations were analyzed: solid lipid nanoparticles loaded or not with atrazine and simazine, SLN; polymeric nanoparticles, NC_PCL loaded with atrazine; and chitosan/tripolyphosphate, CS/TPP, loaded or not with paraquat. All formulations, loaded or not with pesticides, increased lethality in a dose- dependent manner with similar LC50. Both loaded and unloaded NC_PCL were the most toxic formulations to developmental rate, significantly reducing worms length, even at low concentrations. In contrast, both CS/TPP nanoparticles were the least toxic, not affecting reproduction and body length at higher concentrations, probably due to the biocompatibility of chitosan. The physico-chemical characterization of nanoparticles after incubation in saline solution (used in exposure of organisms) has shown that these colloidal systems are stable and remain with the same initial characteristics, even in the presence of saline environment. Notably, our results indicate that the observed effects were caused by the nanoparticles per se. These results suggest that the development of nanoparticles aiming agriculture applications needs more studies in order to optimize the composition and then reduce their toxicity to non-target organisms.

Influence of light, nutrients, and temperature on the toxicity of atrazine to the algal species Raphidocelis subcapitata: Implications for the risk assessment of herbicides.

The acute toxicity of herbicides to algae is commonly assessed under conditions (e.g., light intensity, water temperature, concentration of nutrients, pH) prescribed by standard test protocols. However, the observed toxicity may vary with changes in one or more of these parameters. This study examined variation in toxicity of the herbicide atrazine to a representative green algal species Raphidocelis subcapitata (formerly Pseudokirchneriella subcapitata) with changes in light intensity, water temperature, concentrations of nutrients or combinations of these three parameters. Conditions were chosen that could be representative of the intensive corn growing Midwestern region of the United States of America where atrazine is used extensively. Varying light intensity (4-58µmol/m(2)s) resulted in no observable trend in 96-h EC50 values for growth rate. EC50 values for PSII yield generally increased with decreasing light intensity but not significantly in all cases. The 96-h EC50 values for growth rate decreased with decreases in temperature (20-5°C) from standard conditions (25°C), but EC50 values for PSII yield at lower temperatures were not significantly different from standard conditions. Finally, there was no clear trend in 96-h EC50 values for both endpoints with increases in nitrogen (4.1-20mg/L) and phosphorus (0.24-1.2mg/L). The 96-h EC50 values for both endpoints under combinations of conditions mimicking aquatic systems in the Midwestern U.S. were not significantly different from EC50 values generated under standard test conditions. This combination of decreased light intensity and temperature and increased nutrients relative to standard conditions does not appear to significantly affect the observed toxicity of atrazine to R. subcapitata. For atrazine specifically, and for perhaps other herbicides, this means current laboratory protocols are useful for extrapolating to effects on algae under realistic environmental conditions.

PBPK-Based Probabilistic Risk Assessment for Total Chlorotriazines in Drinking Water.

The risk of human exposure to total chlorotriazines (TCT) in drinking water was evaluated using a physiologically based pharmacokinetic (PBPK) model. Daily TCT (atrazine, deethylatrazine, deisopropylatrazine, and diaminochlorotriazine) chemographs were constructed for 17 frequently monitored community water systems (CWSs) using linear interpolation and Krieg estimates between observed TCT values. Synthetic chemographs were created using a conservative bias factor of 3 to generate intervening peaks between measured values. Drinking water consumption records from 24-h diaries were used to calculate daily exposure. Plasma TCT concentrations were updated every 30 minutes using the PBPK model output for each simulated calendar year from 2006 to 2010. Margins of exposure (MOEs) were calculated (MOE = [Human Plasma TCTPOD] ÷ [Human Plasma TCTEXP]) based on the toxicological point of departure (POD) and the drinking water-derived exposure to TCT. MOEs were determined based on 1, 2, 3, 4, 7, 14, 28, or 90 days of rolling average exposures and plasma TCT Cmax, or the area under the curve (AUC). Distributions of MOE were determined and the 99.9th percentile was used for risk assessment. MOEs for all 17 CWSs were >1000 at the 99.9(th)percentile. The 99.9(th)percentile of the MOE distribution was 2.8-fold less when the 3-fold synthetic chemograph bias factor was used. MOEs were insensitive to interpolation method, the consumer's age, the water consumption database used and the duration of time over which the rolling average plasma TCT was calculated, for up to 90 days. MOEs were sensitive to factors that modified the toxicological, or hyphenated appropriately no-observed-effects level (NOEL), including rat strain, endpoint used, method of calculating the NOEL, and the pharmacokinetics of elimination, as well as the magnitude of exposure (CWS, calendar year, and use of bias factors).

The relationship between study sponsorship, risks of bias, and research outcomes in atrazine exposure studies conducted in non-human animals: Systematic review and meta-analysis.

BACKGROUND: A critical component of systematic review methodology is the assessment of the risks of bias of studies that are included in the review. There is controversy about whether funding source should be included in a risk of bias assessment of animal toxicology studies. OBJECTIVE: To determine whether industry research sponsorship is associated with methodological biases, the results, or conclusions of animal studies examining the effect of exposure to atrazine on reproductive or developmental outcomes. METHODS: We searched multiple electronic databases and the reference lists of relevant articles to identify original research studies examining the effect of any dose of atrazine exposure at any life stage on reproduction or development in non-human animals. We compared methodological risks of bias, the conclusions of the studies, the statistical significance of the findings, and the magnitude of effect estimates between industry sponsored and non-industry sponsored studies. RESULTS: Fifty-one studies met the inclusion criteria. There were no differences in methodological risks of bias in industry versus non-industry sponsored studies. 39 studies tested environmentally relevant concentrations of atrazine (11 industry sponsored, 24 non-industry sponsored, 4 with no funding disclosures). Non-industry sponsored studies (12/24, 50.0%) were more likely to conclude that atrazine was harmful compared to industry sponsored studies (2/11, 18.1%) (p value=0.07). A higher proportion of non-industry sponsored studies reported statistically significant harmful effects (8/24, 33.3%) compared to industry-sponsored studies (1/11; 9.1%) (p value=0.13). The association of industry sponsorship with decreased effect sizes for harm outcomes was inconclusive. CONCLUSION: Our findings support the inclusion of research sponsorship as a risk of bias criterion in tools used to assess risks of bias in animal studies for systematic reviews. The reporting of other empirically based risk of bias criteria for animal studies, such as blinded outcome assessment, randomization, and all animals included in analyses, needs to improve to facilitate the assessment of studies for systematic reviews.