RESUMO
SCOPE: Dietary protein restriction elicits hyperphagia and increases energy expenditure; however, less is known of whether these responses are a consequence of increasing carbohydrate content. The effects of protein-diluted diets with fixed carbohydrate content on energy balance, hormones, and key markers of protein sensing and thermogenesis in tissues are determined. METHODS AND RESULTS: Obesity-prone rats (n = 13-16 per group) are randomized to diets containing fixed carbohydrate (52% calories) and varying protein concentrations: 15% (control), 10% (mild protein restriction), 5% (moderate protein restriction) or 1% (severe protein restriction) protein calories, or protein-matched to 5% protein, for 21 days. Propranolol and ondansetron are administered to interrogate the roles of sympathetic and serotonergic systems, respectively, in diet-induced changes in energy expenditure. It is found that mild-to-moderate protein restriction promotes transient hyperphagia, whereas severe protein restriction induces hypophagia, with alterations in meal patterns. Protein restriction enhances energy expenditure that is partly attenuated by propranolol, but not ondansetron. Moderate to severe protein restriction decreases gains in body weight, lean and fat mass, decreased postprandial glucose and leptin, but increased fibroblast growth factor-21 concentrations. Protein-matching retains lean mass suggesting that intake of dietary protein, but not calories, is important for preserving lean mass. Notably, protein restriction increases the protein and/or transcript abundance of key amino acid sensing molecules in liver and intestine (PERK, eIF2α, ATF2, CHOP, 4EBP1, FGF21), and upregulated thermogenic markers (ß2AR, Klotho, HADH, UCP-1) in brown adipose tissue. CONCLUSION: Low-protein diets promote hyperphagia and sympathetically mediated increase in energy expenditure, prevent gains in tissue reserves, and concurrently upregulate hepatic and intestinal amino acid sensing intermediaries and thermogenic markers in brown adipose tissue.
Assuntos
Dieta com Restrição de Proteínas/efeitos adversos , Metabolismo Energético/efeitos dos fármacos , Hiperfagia/etiologia , Tecido Adiposo Marrom/metabolismo , Animais , Composição Corporal/fisiologia , Peso Corporal , Carboidratos da Dieta/farmacologia , Ingestão de Energia , Fatores de Crescimento de Fibroblastos/sangue , Leptina/sangue , Fígado/metabolismo , Masculino , Ratos Sprague-Dawley , Termogênese/efeitos dos fármacosRESUMO
In this study, a novel water-soluble polysaccharide (RRTP1-1) was obtained from Rosa roxburghii Tratt fruit through the ultrasonic-assisted extraction followed by anion-exchange and size-exclusion chromatography purification. The primary structure of RRTP1-1 was investigated by UV, FT-IR, HPLC, GC-MS, 1H and 13C NMR spectroscopy. Chemical composition analysis revealed that RRTP1-1 had an average molecular weight of 97.58kDa and consisted of Man, Rha, GlcA, GalA, Glc, Gal, Ara and Xyl in a molar ratio of 2.88:1.39:2.83:1.00:69.11:3.04:2.52:3.41. This polysaccharide was proven to be a kind of glucan, owning a backbone structure of â6)-α-d-Glcp-(1â6)-α-d-Glcp-(1â. Congo red test indicated that RRTP1-1 had no triple helix structure. Antioxidation tests performed in vitro revealed that RRTP1-1 possessed obviously DPPH, hydroxyl and superoxide radicals scavenging activities. Furthermore, antioxidant assays in vivo showed that RRTP1-1 at 200 or 400mg/kg dose could significantly enhance the activities of antioxidant enzymes (CAT, SOD, and GSH-Px), increase TAOC values, and decrease the LPO and MDA levels in different degrees in the serum of D-Gal aging-induced mice. The present results suggested that RRTP1-1 could be a new source of natural antioxidants for applications in functional foods and dietary supplemental products.
Assuntos
Antioxidantes/química , Carboidratos da Dieta/farmacologia , Polissacarídeos/química , Rosa/química , Animais , Antioxidantes/farmacologia , Camundongos , Peso Molecular , Monossacarídeos/química , Polissacarídeos/farmacologia , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
BACKGROUND: The carbohydrate-insulin model of obesity posits that habitual consumption of a high-carbohydrate diet sequesters fat within adipose tissue because of hyperinsulinemia and results in adaptive suppression of energy expenditure (EE). Therefore, isocaloric exchange of dietary carbohydrate for fat is predicted to result in increased EE, increased fat oxidation, and loss of body fat. In contrast, a more conventional view that "a calorie is a calorie" predicts that isocaloric variations in dietary carbohydrate and fat will have no physiologically important effects on EE or body fat. OBJECTIVE: We investigated whether an isocaloric low-carbohydrate ketogenic diet (KD) is associated with changes in EE, respiratory quotient (RQ), and body composition. DESIGN: Seventeen overweight or obese men were admitted to metabolic wards, where they consumed a high-carbohydrate baseline diet (BD) for 4 wk followed by 4 wk of an isocaloric KD with clamped protein. Subjects spent 2 consecutive days each week residing in metabolic chambers to measure changes in EE (EEchamber), sleeping EE (SEE), and RQ. Body composition changes were measured by dual-energy X-ray absorptiometry. Average EE during the final 2 wk of the BD and KD periods was measured by doubly labeled water (EEDLW). RESULTS: Subjects lost weight and body fat throughout the study corresponding to an overall negative energy balance of â¼300 kcal/d. Compared with BD, the KD coincided with increased EEchamber (57 ± 13 kcal/d, P = 0.0004) and SEE (89 ± 14 kcal/d, P < 0.0001) and decreased RQ (-0.111 ± 0.003, P < 0.0001). EEDLW increased by 151 ± 63 kcal/d (P = 0.03). Body fat loss slowed during the KD and coincided with increased protein utilization and loss of fat-free mass. CONCLUSION: The isocaloric KD was not accompanied by increased body fat loss but was associated with relatively small increases in EE that were near the limits of detection with the use of state-of-the-art technology. This trial was registered at clinicaltrials.gov as NCT01967563.
Assuntos
Composição Corporal/efeitos dos fármacos , Dieta com Restrição de Carboidratos , Dieta Cetogênica , Carboidratos da Dieta/administração & dosagem , Gorduras na Dieta/administração & dosagem , Metabolismo Energético/efeitos dos fármacos , Obesidade/dietoterapia , Tecido Adiposo/metabolismo , Adulto , Carboidratos da Dieta/metabolismo , Carboidratos da Dieta/farmacologia , Gorduras na Dieta/metabolismo , Gorduras na Dieta/farmacologia , Ingestão de Energia , Humanos , Masculino , Obesidade/metabolismo , Sobrepeso , Redução de PesoRESUMO
BACKGROUND: Closed-loop insulin delivery is an emerging treatment for type 1 diabetes (T1D) evaluated clinically and using computer simulations during pre-clinical testing. Efforts to make closed-loop systems available to people with type 2 diabetes (T2D) calls for the development of a new type of simulators to accommodate differences between T1D and T2D. Presented here is the development of a model of posthepatic endogenous insulin concentration, a component omitted in T1D simulators but key for simulating T2D physiology. METHODS: We evaluated six competing models to describe the time course of endogenous insulin concentration as a function of the plasma glucose concentration and time. The models were fitted to data collected in insulin-naive subjects with T2D who underwent two 24-h visits and were treated, in a random order, by either closed-loop insulin delivery or glucose-lowering oral agents. The model parameters were estimated using a Bayesian approach, as implemented in the WinBUGS software. Model selection criteria were used to identify the best model describing our clinical data. RESULTS: The selected model successfully described endogenous insulin concentration over 24 h in both study periods and provided plausible parameter estimates. Model-derived results were in concordance with a clinical finding which revealed increased posthepatic endogenous insulin concentration during the control study period (P < 0.05). The modelling results indicated that the excess amount of insulin can be attributed to the glucose-independent effect as the glucose-dependent effect was similar between visits (P > 0.05). CONCLUSIONS: A model to describe endogenous insulin concentration in T2D including components of posthepatic glucose-dependent and glucose-independent insulin secretion was identified and validated. The model is suitable to be incorporated in a simulation environment for evaluating closed-loop insulin delivery in T2D.
Assuntos
Simulação por Computador , Diabetes Mellitus Tipo 2/sangue , Sistemas de Infusão de Insulina , Insulina Lispro/administração & dosagem , Insulina/sangue , Modelos Teóricos , Pâncreas Artificial , Administração Oral , Teorema de Bayes , Glicemia/análise , Diabetes Mellitus Tipo 2/tratamento farmacológico , Carboidratos da Dieta/farmacologia , Humanos , Hipoglicemiantes/administração & dosagem , Hipoglicemiantes/uso terapêutico , Insulina/metabolismo , Secreção de Insulina , Cadeias de Markov , Período Pós-PrandialRESUMO
Beneficial alleles that spread rapidly as an adaptation to a new environment are often associated with costs that reduce the fitness of the population in the original environment. Several species of insect pests have evolved resistance to Bacillus thuringiensis (Bt) toxins in the field, jeopardizing its future use. This has most commonly occurred through the alteration of insect midgut binding sites specific for Bt toxins. While fitness costs related to Bt resistance alleles have often been recorded, the mechanisms behind them have remained obscure. We asked whether evolved resistance to Bt alters dietary nutrient intake, and if reduced efficiency of converting ingested nutrients to body growth are associated with fitness costs and variation in susceptibility to Bt. We fed the cabbage looper Trichoplusia ni artificial diets differing in levels of dietary imbalance in two major macronutrients, protein and digestible carbohydrate. By comparing a Bt-resistant T. ni strain with a susceptible strain we found that the mechanism behind reduced pupal weights and growth rates associated with Bt-resistance in T. ni was reduced consumption rather than impaired conversion of ingested nutrients to growth. In fact, Bt-resistant T. ni showed more efficient conversion of nutrients than the susceptible strain under certain dietary conditions. Although increasing levels of dietary protein prior to Bt challenge had a positive effect on larval survival, the LC50 of the resistant strain decreased when fed high levels of excess protein, whereas the LC50 of the susceptible strain continued to rise. Our study demonstrates that examining the nutritional basis of fitness costs may help elucidate the mechanisms underpinning them.
Assuntos
Bacillus thuringiensis/fisiologia , Dieta , Resistência a Inseticidas , Mariposas/microbiologia , Fenômenos Fisiológicos da Nutrição Animal , Animais , Bacillus thuringiensis/química , Toxinas Bacterianas/metabolismo , Brassica/parasitologia , Carboidratos da Dieta/farmacologia , Proteínas Alimentares/farmacologia , Interações Hospedeiro-Patógeno , Mariposas/crescimento & desenvolvimento , Pupa/efeitos dos fármacos , Pupa/crescimento & desenvolvimento , Pupa/microbiologiaRESUMO
Obesity-prone (OP) rodents are used as models of human obesity predisposition. The goal of the present study was to identify preexisting defects in energy expenditure components in OP rats. Two studies were performed. In the first one, male Wistar rats (n = 48) were fed a high-carbohydrate diet (HCD) for 3 wk and then a high-fat diet (HFD) for the next 3 wk. This study showed that adiposity gain under HCD was 2.9-fold larger in carbohydrate-sensitive (CS) than in carbohydrate-resistant (CR) rats, confirming the concept of "carbohydrate-sensitive" rats. Energy expenditure (EE), respiratory quotient (RQ), caloric intake (CI), and locomotor activity measured during HFD identified no differences in EE and RQ between fat-resistant (FR) and fat-sensitive (FS) rats, and indicated that obesity developed in FS rats only as the result of a larger CI not fully compensated by a parallel increase in EE. A specific pattern of spontaneous activity, characterized by reduced activity burst intensity, was identified in FS rats but not in CS ones. This mirrors a previous observation that under HCD, CS but not FS rats, exhibited bursts of activity of reduced intensity. In a second study, rats were fed a HFD for 3 wk, and the components of energy expenditure were examined by indirect calorimetry in 10 FR and 10 FS rats. This study confirmed that a low basal EE, reduced thermic effect of feeding, defective postprandial energy partitioning, or a defective substrate utilization by the working muscle are not involved in the FS phenotype.
Assuntos
Carboidratos da Dieta/farmacologia , Gorduras na Dieta/farmacologia , Ingestão de Alimentos/efeitos dos fármacos , Metabolismo Energético/efeitos dos fármacos , Obesidade/genética , Obesidade/fisiopatologia , Animais , Composição Corporal/efeitos dos fármacos , Composição Corporal/fisiologia , Peso Corporal/efeitos dos fármacos , Peso Corporal/fisiologia , Calorimetria Indireta , Modelos Animais de Doenças , Ingestão de Alimentos/fisiologia , Metabolismo Energético/fisiologia , Predisposição Genética para Doença/genética , Locomoção/efeitos dos fármacos , Locomoção/fisiologia , Masculino , Ratos , Ratos WistarAssuntos
Proteínas Alimentares/farmacologia , Indústria Alimentícia , Resposta de Saciedade/fisiologia , Animais , Evolução Biológica , Carboidratos da Dieta/economia , Carboidratos da Dieta/farmacologia , Gorduras na Dieta/economia , Gorduras na Dieta/farmacologia , Proteínas Alimentares/economia , Indústria Alimentícia/economia , Humanos , Resposta de Saciedade/efeitos dos fármacosRESUMO
The purpose of the present study was to investigate whether whey protein (WP), casein protein (CP), carbohydrate (CHO) or a non-energy-containing placebo (PLA) consumed before sleep alters morning appetite and resting energy expenditure (REE) in active men. A total of eleven men (age: 23·6 (sem 1·0) years; body fat: 16·3 (sem 2·5) %) participated in this randomised, double-blind, cross-over study. A single dose of WP (30 g), CP (30 g), CHO (33 g) or PLA was consumed 30 min before sleep, and each trial was separated by 48-72 h. The next morning (05.00-08.00 hours), measurements of satiety, hunger and desire to eat and REE were taken. After a 30 min equilibration period, REE in the supine position was measured for 60 min. An analysis of 10 min mean intervals over the final 50 min of the measurement period was conducted. Statistical analyses were conducted using repeated-measures ANOVA for metabolic variables, and a one-way ANOVA was used for measuring changes in appetite markers. Group differences were examined by Tukey's post hoc analysis. There were no significant differences in appetite measures among the groups. There was a main group effect for REE. The predicted REE was significantly greater after consumption of the WP (8151 (sem 67) kJ/d), CP (8126 (sem 67) kJ/d) and CHO (7988 (sem 67) kJ/d) than after that of the PLA (7716 (sem 67) kJ/d, P <0·0001). There were no significant differences between the WP and CP groups in any metabolic measurements. Night-time consumption of WP, CP or CHO, in the hours close to sleep, elicits favourable effects on the next-morning metabolism when compared with that of a PLA in active young men.
Assuntos
Apetite/efeitos dos fármacos , Metabolismo Basal/efeitos dos fármacos , Caseínas/farmacologia , Carboidratos da Dieta/farmacologia , Proteínas Alimentares/farmacologia , Proteínas do Leite/farmacologia , Lanches , Adulto , Análise de Variância , Composição Corporal , Estudos Cross-Over , Método Duplo-Cego , Ingestão de Energia/efeitos dos fármacos , Humanos , Masculino , Descanso , Saciação/efeitos dos fármacos , Sono , Proteínas do Soro do Leite , Adulto JovemRESUMO
The R230C variant of the ATP-binding cassette transporter A1 (ABCA1) gene has been consistently associated with decreased HDL-cholesterol (HDL-C) concentrations in several studies in the Mexican mestizo population. However, information on how diet composition modifies the effect of the ABCA1-R230C variant on HDL-C concentrations is very scarce. The aim of the present study was to analyze whether the effect of ABCA1-R230C on HDL-C concentrations is modulated by dietary factors in a nationwide population sample of 3591 adults from the National Health and Nutrition Survey conducted by the State's Employees' Social Security and Social Services Institute. All participants answered a validated questionnaire to assess health status and weekly food consumption. Fasting blood samples were drawn for biochemical analysis and DNA extraction, and the ABCA1-R230C variant was genotyped using TaqMan assays. Statistical analyses consisted of simple linear and multiple regression modeling adjusting for age, BMI, smoking, and alcohol consumption. The overall C risk allele frequency was 9.3% and the variant was significantly associated with low HDL-C concentrations in both sexes. A significant negative correlation between carbohydrate consumption and HDL-C concentrations was observed in women bearing the R230C variant (P = 0.021) and a significant gene-diet interaction was found only in premenopausal women (P = 0.037). In conclusion, the effect of the ABCA1-R230C gene variant on HDL-C concentrations is modulated by carbohydrate intake in premenopausal women. This finding may help design optimized dietary interventions according to sex and ABCA1-R230C genotype.
Assuntos
Transportadores de Cassetes de Ligação de ATP/genética , Transportadores de Cassetes de Ligação de ATP/metabolismo , HDL-Colesterol/sangue , Carboidratos da Dieta/administração & dosagem , Variação Genética , Pré-Menopausa , Transportador 1 de Cassete de Ligação de ATP , Adulto , Alelos , Estudos Transversais , Inquéritos sobre Dietas , Carboidratos da Dieta/farmacologia , Feminino , Genótipo , Humanos , Masculino , México , Pessoa de Meia-Idade , Pré-Menopausa/sangue , Pré-Menopausa/genética , Fatores de Risco , Caracteres Sexuais , Inquéritos e QuestionáriosRESUMO
High protein (HP) diet could serve as a good strategy against obesity, provoking the changes in energy metabolic pathways. However, those modifications differ during a dietary adaptation. To better understand the mechanisms involved in effect of high protein diet (HP) on limiting adiposity in rats we studied in parallel the gene expression of enzymes involved in protein and energy metabolism and the profiles of nutrients oxidation. Eighty male Wistar rats were fed a normal protein diet (NP, 14% of protein) for one week, then either maintained on NP diet or assigned to a HP diet (50% of protein) for 1, 3, 6 and 14 days. mRNA levels of genes involved in carbohydrate and lipid metabolism were measured in liver, adipose tissues, kidney and muscles by real time PCR. Energy expenditure (EE) and substrate oxidation were measured by indirect calorimetry. Liver glycogen and plasma glucose and hormones were assayed. In liver, HP feeding 1) decreased mRNA encoding glycolysis enzymes (GK, L-PK) and lipogenesis enzymes(ACC, FAS), 2) increased mRNA encoding gluconeogenesis enzymes (PEPCK), 3) first lowered, then restored mRNA encoding glycogen synthesis enzyme (GS), 4) did not change mRNA encoding ß-oxidation enzymes (CPT1, ACOX1, ßHAD). Few changes were seen in other organs. In parallel, indirect calorimetry confirmed that following HP feeding, glucose oxidation was reduced and fat oxidation was stable, except during the 1(st) day of adaptation where lipid oxidation was increased. Finally, this study showed that plasma insulin was lowered and hepatic glucose uptake was decreased. Taken together, these results demonstrate that following HP feeding, CHO utilization was increased above the increase in carbohydrate intake while lipogenesis was decreased thus giving a potential explanation for the fat lowering effect of HP diets.
Assuntos
Metabolismo dos Carboidratos/efeitos dos fármacos , Carboidratos da Dieta/farmacologia , Proteínas Alimentares/farmacologia , Glucose/metabolismo , Ração Animal , Animais , Metabolismo dos Carboidratos/fisiologia , Dieta com Restrição de Carboidratos/efeitos adversos , Metabolismo dos Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Modelos Biológicos , Oxirredução/efeitos dos fármacos , Ratos , Ratos Wistar , Regulação para CimaRESUMO
The objective of this study was to assess how short-term feeding of high levels of dietary medium-chain triglyceride (MCT) affect energy expenditure and postprandial substrate oxidation rates in normal-weight, premenopausal women. Eight healthy women were fed both a MCT-rich and an isocaloric long-chain triglyceride (LCT)-rich diet for two 1-week periods separated by a minimum of 21 days. The energy intake in each diet was 45% carbohydrates, 40% fat, and 15% protein. The 2 diets had either 60.81% or 1.11% of total fat energy from MCT with the remaining fat energy intake from LCT. On days 1 and 7 of each diet, resting metabolic rate and postprandial energy expenditure (EE) were measured by indirect calorimetry with a ventilated hood. Results indicated on days 1 and 7, there were no significant differences between diets for resting metabolic rate or mean postprandial EE. On both days 1 and 7, fat oxidation for the MCT-rich diet was significantly greater (0.0001 = p = 0.04) than that for the LCT-rich diet at different time points across the 5.5 h postprandial period. In conclusion, for premenopausal, normal-weight women consuming a diet with 25% of the energy content from MCT, there were no changes in resting metabolic rate, transient increases in postprandial energy expenditure, and significant increases in postprandial fat oxidation.
Assuntos
Peso Corporal/efeitos dos fármacos , Gorduras na Dieta/farmacologia , Metabolismo Energético/efeitos dos fármacos , Triglicerídeos/farmacologia , Adulto , Análise de Variância , Metabolismo Basal/efeitos dos fármacos , Índice de Massa Corporal , Calorimetria Indireta/métodos , Carboidratos da Dieta/administração & dosagem , Carboidratos da Dieta/farmacologia , Gorduras na Dieta/administração & dosagem , Proteínas Alimentares/administração & dosagem , Proteínas Alimentares/farmacologia , Ingestão de Energia/efeitos dos fármacos , Feminino , Humanos , Consumo de Oxigênio/efeitos dos fármacos , Período Pós-Prandial , Troca Gasosa Pulmonar/efeitos dos fármacos , Fatores de Tempo , Triglicerídeos/administração & dosagem , Triglicerídeos/químicaRESUMO
BACKGROUND: It has been suggested that the satiating power of the 4 macronutrients follows the oxidation hierarchy: alcohol > protein > carbohydrate > fat. However, the experimental evidence for this is still scarce. OBJECTIVE: The goal was to investigate the effects on appetite, energy intake and expenditure, and substrate metabolism of meals rich in 1 of the 4 macronutrients. DESIGN: Subjective appetite sensations, ad libitum food intake, energy expenditure, substrate metabolism, and hormone concentrations were measured for 5 h after breakfast meals with similar energy density and fiber contents but rich in either protein (32% of energy), carbohydrate (65% of energy), fat (65% of energy), or alcohol (23% of energy). Subjects were normal-weight, healthy women (n = 9) and men (n = 10) studied in a crossover design. RESULTS: There were no significant differences in hunger or satiety sensations or in ad libitum energy intake after the 4 meals. Diet-induced thermogenesis was larger after the alcohol meal (by 27%; P < 0.01), whereas protein produced an intermediary response (17%; NS) compared with carbohydrate and fat (meal effect: P < 0.01). After the alcohol meal, fat oxidation and leptin concentrations were greatly suppressed (meal effects, P < 0.0001 and P < 0.05) and triacylglycerol concentrations were as high as after the fat meal. CONCLUSION: Intake of an alcohol-rich meal stimulates energy expenditure but suppresses fat oxidation and leptin more than do isoenergetically dense meals rich in protein, carbohydrate, or fat. Despite differences in substrate metabolism and hormone concentrations, satiety and ad libitum energy intake were not significantly different between meals. Our data, therefore, do not support the proposed relation between the macronutrient oxidation hierarchy and the satiety hierarchy.
Assuntos
Apetite/efeitos dos fármacos , Carboidratos da Dieta/farmacologia , Gorduras na Dieta/farmacologia , Proteínas Alimentares/farmacologia , Metabolismo Energético/efeitos dos fármacos , Etanol/farmacologia , Adulto , Análise de Variância , Glicemia/efeitos dos fármacos , Calorimetria Indireta , Carboidratos da Dieta/administração & dosagem , Gorduras na Dieta/administração & dosagem , Proteínas Alimentares/administração & dosagem , Ingestão de Energia/efeitos dos fármacos , Etanol/administração & dosagem , Feminino , Humanos , Masculino , Saciação/efeitos dos fármacosRESUMO
We examined the costs associated with acclimation in an omnivorous mouse (Phyllotis darwini) fed two contrasting diets (carbohydrate-rich and protein-rich). We studied the response of gut morphology and digestive performance in animals shifted to a novel diet at different developmental stages. When acclimated adult animals were shifted to the alternative diet, energy digestibility decreased. We also found long-term consequences to diet acclimation. Animals reacclimated for 15 days to an alternative diet did not increase digestive performance. Although no effects of diet on gut morphology were noted, a significant positive correlation between energy digestibility and small intestinal length was found, explaining most of the variability observed in energy digestibility. These results suggest that caution should be used when defining adaptive changes if the possible cost of acclimation is neglected.
Assuntos
Aclimatação/fisiologia , Adaptação Fisiológica/fisiologia , Fenômenos Fisiológicos da Nutrição Animal , Digestão/fisiologia , Aclimatação/efeitos dos fármacos , Adaptação Fisiológica/efeitos dos fármacos , Fatores Etários , Animais , Peso Corporal/fisiologia , Cricetinae , Dieta , Carboidratos da Dieta/farmacologia , Proteínas Alimentares/farmacologia , Digestão/efeitos dos fármacos , Metabolismo Energético/efeitos dos fármacos , Metabolismo Energético/fisiologia , Feminino , Camundongos , Gravidez , Análise de RegressãoRESUMO
The effects of gastric infusions of fat and carbohydrate on physiological and psychological measures were compared using a within subject design in 9 healthy subjects (6 males). Each subject received isovolaemic rapid gastric infusions of sucrose solution (100% energy carbohydrate), lipid emulsion (100% energy fat, 20% Intralipid), and a non-nutrient control (0.9% saline) in a randomised order. Nutrient infusions were isoenergetic, containing one-third of an individual subject's estimated daily energy requirements (mean, 3227 kJ; range, 2479-3971 kJ). Measures of heart rate (HR), energy expenditure (EE), mood, and sleepiness were collected before the infusions and every 0.5 h for 3.5 h. Mean postingestive HR, EE, and satiation were significantly greater after the nutrient infusions than after the control. Sucrose induced a rapid increase in HR and EE, whereas lipid had a lesser and more delayed effect. Thirty minutes after the gastric infusions, HR and EE were significantly higher after the sucrose than after the lipid and saline. Hedonic tone was greater and tension lower after the saline and sucrose infusions than after the lipid infusion. From 3 to 3.5 h after ingestion, subjects felt significantly more sleepy after the lipid infusion than they did at these times after the saline infusion, and significantly more dreamy after the lipid infusion than they did after the sucrose infusion. In conclusion, the presence of lipid and sucrose in the intestine induces significant and differing physiological and psychological effects, which are independent of cognitive and orosensory influences.
Assuntos
Afeto/fisiologia , Carboidratos da Dieta/farmacologia , Gorduras na Dieta/farmacologia , Metabolismo Energético/efeitos dos fármacos , Frequência Cardíaca/fisiologia , Fases do Sono/fisiologia , Adulto , Afeto/efeitos dos fármacos , Feminino , Frequência Cardíaca/efeitos dos fármacos , Humanos , Intubação Gastrointestinal , Masculino , Mecânica Respiratória , Resposta de Saciedade/efeitos dos fármacos , Fases do Sono/efeitos dos fármacosAssuntos
Carboidratos da Dieta/farmacologia , Ingestão de Energia/fisiologia , Metabolismo Energético/fisiologia , Obesidade/fisiopatologia , Peso Corporal/fisiologia , Causalidade , Carboidratos da Dieta/metabolismo , Gorduras na Dieta/metabolismo , Humanos , Incidência , Obesidade/epidemiologia , Obesidade/metabolismo , OxirreduçãoRESUMO
An in vitro method simulating conditions in the small intestine and colon was used to study the effects of various carbohydrates on Ca release from basal diet (BD) containing dairy products. During enzymatic digestion of BD, 28.5 +/- 0.3% of the Ca was released. This was reduced by 3, 22 and 27% by adding bread, psyllium or pectin to BD, respectively (P < 0.05). After enzymatic digestion, the residue from BD was fermented releasing 11.9 +/- 1.2% of the Ca, a value which was significantly less than with pectin (13.7 +/- 0.9%) and greater than with psyllium (4.4 +/- 0.2%) addition. The total Ca release ranged from 26.5 +/- 0.8 to 42.2 +/- 1.0% with bread>BD>pectin>psyllium. Lactulose did not differ significantly from BD. These results suggest that carbohydrates may bind Ca and reduce its availability for absorption in the small intestine. However, if the carbohydrate is fermented, bound Ca may be released for potential absorption in the colon, whereas less fermented carbohydrates may continue to bind Ca in the colon. The in vitro method described may be useful for estimating total Ca availability. However, studies in humans are required to validate these results.
Assuntos
Cálcio/farmacocinética , Colo/fisiologia , Carboidratos da Dieta/farmacologia , Absorção Intestinal/fisiologia , Intestino Delgado/fisiologia , Disponibilidade Biológica , Cálcio/metabolismo , Colo/metabolismo , Dieta/normas , Carboidratos da Dieta/metabolismo , Feminino , Fermentação , Humanos , Intestino Delgado/metabolismo , Lactulose/metabolismo , Lactulose/farmacologia , Avaliação Nutricional , Pectinas/metabolismo , Pectinas/farmacologia , Psyllium/metabolismo , Psyllium/farmacologiaRESUMO
Because resistant starch (RS) is not absorbed as glucose in the small intestine of healthy humans, postprandial thermogenesis should be lower after the intake of RS as compared with digestible starch. To evaluate this hypothesis, we measured 5-h postprandial thermogenesis and substrate oxidation by indirect calorimetry after ingestion of 50 g pregelatinized (0% RS) and 50 g raw potato starch (54% type II RS) in 15 healthy, normal-weight young males. The subjects consumed each starch (mixed in diluted fruit syrup) twice on separate days and in random order. RS intake was followed by lower thermogenesis (46.5 +/- 13.1 compared with 115.4 +/- 10.4 kJ/5 h; P = 0.008), lower glucose oxidation (P < 0.0005), and greater fat oxidation (P = 0.013) than was pregelatinized starch consumption. Our results suggest that RS has no thermogenic effect and that its presence does not influence the size of the thermic response to digestible starch.
Assuntos
Regulação da Temperatura Corporal/efeitos dos fármacos , Carboidratos da Dieta/farmacologia , Metabolismo Energético/efeitos dos fármacos , Solanum tuberosum , Amido/farmacologia , Adulto , Composição Corporal/fisiologia , Regulação da Temperatura Corporal/fisiologia , Calorimetria Indireta , Estudos Cross-Over , Carboidratos da Dieta/metabolismo , Digestão , Metabolismo Energético/fisiologia , Humanos , Intestino Delgado/metabolismo , Masculino , Oxirredução , Amido/metabolismo , Fatores de TempoRESUMO
To test the benefits of aerobic exercise and dietary carbohydrate during reduced-energy feeding, 23 obese women (44 +/- 4% fat) were randomly assigned to either aerobic exercise (Ex) or no exercise (Nx), and to a low-fat (LF) or low-carbohydrate (LC) reducing diet (5.00 +/- 0.56 MJ/d) for 12 wk. Changes in body composition, postabsorptive resting metabolic rate (RMR), thermic effect of a meal (TEM), and total daily energy expenditure (TDEE) were measured by respiratory gas exchange and doubly labeled water. Significant effects of Ex included a greater loss of fat mass (Ex: -8.8 +/- 2.1 vs Nx: -6.1 +/- 2.3 kg, P = 0.008) and maintenance of TDEE (Ex: +0.07 +/- 1.23 vs Nx: -1.46 +/- 1.04 MJ/d, P = 0.004), due to a difference in physical activity (Ex: +0.75 +/- 1.06 vs Nx: -0.61 +/- 1.03 MJ/d, P = 0.006), which was not attributable solely to the Ex sessions. RMR in both groups decreased comparably (-0.54 MJ/d), and TEM (% of meal) did not change. Diet composition did not significantly influence body composition or energy expenditure changes, but a greater weight loss was observed after the LC than after the LF (-10.6 +/- 2.0 vs -8.1 +/- 3.0 kg, P = 0.037) diet. The addition of aerobic exercise to a low-energy diet was beneficial in the treatment of moderate obesity because of its favorable effects on body composition, physical activity, and TDEE.
Assuntos
Carboidratos da Dieta/farmacologia , Exercício Físico , Obesidade/terapia , Redução de Peso , Adulto , Composição Corporal , Ingestão de Energia , Metabolismo Energético , Feminino , Humanos , Pessoa de Meia-Idade , Obesidade/metabolismoRESUMO
Whether or not a high intake of carbohydrate increases postprandial energy expenditure during exercise was studied in rats. The rats were meal-fed regularly twice a day (0800-0900 hours and 1800-1900 hours) on either a high carbohydrate (CHO) (carbohydrate/fat/protein = 70/5/25, % of energy) or high fat (FAT) (35/40/25) diet for 12 days. On the final day of the experiment, all of the rats in each dietary group were fed an evening meal containing equal amounts of energy (420 kJ.kg-1 body mass). After the meal, they were divided into three subgroups: pre-exercise control (PC), exercise (EX), and resting control (RC). The PC-CHO and PC-FAT groups were sacrificed at 2030 hours. The EX-CHO and EX-FAT groups were given a period of 3-h swimming, and then sacrificed at 2330 hours. The RC-CHO and RC-FAT groups rested after the meal and were sacrificed at 2330 hours. Total energy expenditure during the period 1.5 h from the commencement of exercise was higher in EX-CHO than in EX-FAT. The respiratory exchange ratio was also higher in EX-CHO than in EX-FAT, suggesting enhanced carbohydrate oxidation in the former. Compared with both PC-FAT and RC-FAT, the liver glycogen content of EX-FAT rats was significantly decreased by exercise. On the other hand, the liver glycogen content of both EX-CHO and RC-CHO was higher than that of PC-CHO rats. The glycogen content of soleus muscle of EX-FAT was slightly decreased during exercise, however, that of EX-CHO increased significantly.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Carboidratos da Dieta/farmacologia , Metabolismo Energético/efeitos dos fármacos , Esforço Físico/fisiologia , Animais , Peso Corporal/fisiologia , Dieta , Gorduras na Dieta/farmacologia , Ingestão de Alimentos , Epinefrina/urina , Glicogênio/metabolismo , Glicogênio Hepático/metabolismo , Masculino , Músculos/efeitos dos fármacos , Músculos/metabolismo , Norepinefrina/urina , Tamanho do Órgão/fisiologia , Consumo de Oxigênio/efeitos dos fármacos , Troca Gasosa Pulmonar/fisiologia , Ratos , Ratos Sprague-DawleyRESUMO
The influence of dietary composition on whole-body energetics was examined during the first 2 weeks of isocaloric refeeding after low food intake in a rat model. The high energetic efficiency and energy partitioning toward fat accretion characteristic of this refeeding period were unaltered by (1) dietary fat levels varying between 6% and 30% of energy intake; (2) protein levels between 15% and 40%; (3) carbohydrate types (glucose v fructose v sucrose v starch v unrefined carbohydrate); and (4) diets containing 30% fat but differing in fatty acid composition (long-chain triglycerides [LCT] v medium-chain triglycerides [MCT] v oleic v linoleic v alpha-linolenic metabolites eicosapentaenoic acid [EPA] and docosahexaenoic acid [DHA] omega-3 fatty acids). Changes were only observed for extreme diets, ie, those deficient in protein or very high in fat. Low-protein diet was the only condition in which the high metabolic efficiency characteristic of the refeeding period was partially suppressed, and this occurred despite a lack of concomitant reduction in body fat deposition. On the contrary, with high-fat diets (> 30% of dietary energy consumption) the elevated efficiency was further increased, an effect that was only partially accounted for by the lower energy cost of body fat gain from high-fat diets. These studies indicate that during body weight recovery, the mechanisms underlying the adaptive increase in metabolic efficiency favoring the replenishment of body fat stores override any effect of food type on thermogenesis, and suggest some convergence in the controlling neural pathway. The implications of these findings vis-a-vis nutritional rehabilitation (catch-up growth) and obesity relapse are discussed.