Assessment of the Efficacy and Bond Strength of Different Dentin-bonding Agents with Adhesives on Primary Teeth: An In Vitro Study.

AIM: The purpose of this study was to evaluate the effectiveness and strength of three various dentin-bonding agents used with adhesives on primary teeth. MATERIALS AND METHODS: The study used 80 recently extracted, healthy human maxillary anterior primary teeth that had undergone physiologic resorption, or over-retention. Teeth were cut to expose a flat dentin surface at a depth of 1.5 mm. All samples were divided into four groups (20 samples in each group) as follows: Group I-Control group, Group II-Primary teeth bonding with 6th-generation bonding agent, Group III-Primary teeth bonding with 7th-generation bonding agent, Group IV-Primary teeth bonding with 8th-generation bonding agent. All of the samples' dentinal surfaces were covered with composite resin using a Teflon mold after adhesive had been applied. A universal testing machine (INSTRON) was used to assess the shear bond strength. Data were collected and statistically analyzed. RESULTS: The maximum mean shear bond strength was found in 8th-generation bonding agent (30.76 ± 0.16), followed by 7th-generation bonding agent (26.08 ± 0.21), 6th-generation bonding agent (25.32 ± 0.06), and control group (6.18 ± 0.09). Statistically significant difference was found between the three different bonding agents (p < 0.001). CONCLUSION: On conclusion, the 8th-generation bonding agent demonstrated a greater shear bond strength to dentin than the 7th and 6th-generation bonding agent. CLINICAL SIGNIFICANCE: The emergence of different bonding techniques to the market improves the durability and quality of restorations. An effective bonding to the tooth would also reduce bacterial penetration, marginal microleakage, possibility of pulpal inflammation preserve tooth structure, and postoperative sensitivity by allowing less cavity preparation. How to cite this article: Alqarni AS, Al Ghwainem A. Assessment of the Efficacy and Bond Strength of Different Dentin-bonding Agents with Adhesives on Primary Teeth: An In Vitro Study. J Contemp Dent Pract 2024;25(4):342-345.

Aluminum zirconate nanoparticles in etch and rinse adhesive to caries affected dentine: An in-vitro scanning electron microscopy, elemental distribution, antibacterial, degree of conversion and micro-tensile bond strength assessment.

To incorporate different concentrations of Al2O9Zr3 (1%, 5%, and 10%) nanoparticles (NP) into the ER adhesive and subsequently assess the impact of this addition on the degree of conversion, µTBS, and antimicrobial efficacy. The current research involved a wide-ranging examination that merged various investigative techniques, including the application of scanning electron microscopy (SEM) for surface characterization of NP coupled with energy-dispersive x-ray spectroscopy (EDX), Fourier-transform infrared (FTIR) spectroscopy, µTBS testing, and microbial analysis. Teeth were divided into four groups based on the application of modified and unmodified three-step ER adhesive primer. Group 1 (0% Al2O9Zr3 NPs) Control, Group 2 (1% Al2O9Zr3 NPs), Group 3 (5% Al2O9Zr3 NPs), and Group 4 (10% Al2O9Zr3 NPs). EDX analysis of Al2O9Zr3 NPs was performed showing elemental distribution in synthesized NPs. Zirconium (Zr), Aluminum (Al), and Oxides (O2). After primer application, an assessment of the survival rate of Streptococcus mutans was completed. The FTIR spectra were analyzed to observe the characteristic peaks indicating the conversion of double bonds, both before and after the curing process, for the adhesive Etch and rinse containing 1,5,10 wt% Al2O9Zr3 NPs. µTBS and failure mode assessment were performed using a Universal Testing Machine (UTM) and stereomicroscope respectively. The µTBS and S.mutans survival rates comparison among different groups was performed using one-way ANOVA and Tukey post hoc (p = .05). Group 4 (10 wt% Al2O9Zr3 NPs + ER adhesive) specimens exhibited the minimum survival of S.mutans (0.11 ± 0.02 CFU/mL). Nonetheless, Group 1 (0 wt% Al2O9Zr3 NPs + ER adhesive) displayed the maximum surviving S.mutans (0.52 ± 0.08 CFU/mL). Moreover, Group 2 (1 wt% Al2O9Zr3 NPs + ER adhesive) (21.22 ± 0.73 MPa) samples displayed highest µTBS. However, the bond strength was weakest in Group 1 (0 wt% Al2O9Zr3 NPs + ER adhesive) (14.13 ± 0.32 MPa) study samples. The etch-and-rinse adhesive exhibited enhanced antibacterial activity and micro-tensile bond strength (µTBS) when 1% Al2O9Zr3 NPs was incorporated, as opposed to the control group. Nevertheless, the incorporation of Al2O9Zr3 NPs led to a decrease in DC. RESEARCH HIGHLIGHTS: 10 wt% Al2O9Zr3 NPs + ER adhesive specimens exhibited the minimum survival of S.mutans. 1 wt% Al2O9Zr3 NPs + ER adhesive samples displayed the most strong composite/CAD bond. The highest DC was observed in Group 1: 0 wt% Al2O9Zr3 NPs + ER adhesive.

Assessment of physical properties of bioactive glass-modified universal multimode adhesive and its bonding potential to artificially induced caries affected dentin.

BACKGROUND: To evaluate the physical properties of bioactive glass-modified universal multimode adhesive and its micro-tensile bond strength (µTBS) to artificially induced caries-affected dentin. METHODS: All bond universal adhesive was used in the study. Specimens were divided into 2 main groups: control unmodified adhesive and 5 wt% BAG modified adhesive. The degree of conversion, pH, bioactivity, and viscosity of the adhesives were tested with n = 5 for each test. Micro-tensile bond strength evaluation was done in etch & rinse (ER) and selective-etch (SE) modes, where 24 human molar teeth were used (n = 3), 12 teeth for immediate bond strength, and the other 12 were tested after 6 months of storage in simulated body fluid (SBF). RESULTS: No significant difference was found between the control and the 5wt% BAG groups regarding the degree of conversion (61.01 ± 0.43 and 60.44 ± 0.61 respectively) and the viscosity (109.77 ± 22.3 and 124.3 ± 9.92 respectively). The control group revealed significantly lower pH values than the 5wt% BAG group (3.16 ± 0.5 and 4.26 ± 0.09 respectively). Immediate bond strength results revealed that the 5wt% BAG in the ER mode had the highest bond strength followed by the control group in the ER mode (44.16 ± 7.53 and 44.00 ± 7.96 respectively). SE groups showed that the immediate strength of the 5wt% BAG group was higher than the control group (42.09 ± 6.02 and 39.29 ± 6.64 respectively). After 6 months of storage, bond strength results revealed a decrease in bond strength values for the control groups but not for the 5wt% BAG in both application modes. CONCLUSIONS: The incorporation of BAG (5wt%) improved the universal adhesive micro-tensile bond strength and bond durability for both adhesive application modes without affecting its degree of conversion or viscosity.

Noninvasive assessment of novel nanohybrid resin cement adaptation using cross-polarization optical coherence tomography.

OBJECTIVES: Zein-coated magnesium oxide nanoparticles (zMgO NPs) can potentially improve cement adaptation to the tooth-restoration interface, which would aid in minimizing marginal leakage and secondary caries. The aim of this study was to assess the effect of incorporating zMgO NPs on the adaptation of self-adhesive resin cement using cross-polarization optical coherence tomography (CP-OCT) and scanning electron microscopy (SEM). METHODS: Resin inlays were fabricated to be cemented in Class-I cavities of extracted human molars. All specimens were randomly divided into five groups (n = 10), and the resin inlays were cemented using self-adhesive resin cement with various concentrations of zMgO NPs (0% [control], 0.3%, 0.5%, 1%, 2%). Characterization was done by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and SEM. The specimens were examined for interfacial adaptation under CP-OCT. Floor and wall adaptation measurements were analyzed by software on 20 B-scans, and samples were sectioned for interfacial measurement by SEM. RESULTS: Results for CP-OCT and SEM showed a statistically significant increase of adaptation in the floor and wall of resin cement filled with zMgO NPs compared to the control. The samples enhanced with 0.3% and 0.5% showed a statistically significantly better adaptation in floor and wall in CP-OCT and SEM. However, there was no significant difference between the 1%, 2%, and control groups for CP-OCT and SEM analysis. SIGNIFICANCE: The incorporation of zMgO NPs in self-adhesive resin cement can enhance the cement's properties by significantly improving its wall and floor adaptation.

Characterization and Assessment of Physical Properties of 3 Single Syringe Hydraulic Cement-based Sealers.

INTRODUCTION: A number of sealers with different chemistries are badged as Bioceramic, implying biological activity, but have dissimilar properties, which has implications on the sealer properties and will affect the quality and outcome of root canal treatment. This study aimed to assess the physical and chemical properties of 3 hydraulic cement-based sealers, namely BC Universal sealer compared with Totalfill BC sealer and AH Plus Bioceramic. METHODS: The microstructure and composition of the sealers were assessed using scanning electron microscopy and energy dispersive spectroscopy after setting. The crystalline phases were assessed by X-ray diffraction analysis and the leachates were tested using inductively coupled plasma. All testing was performed at 0, 7, and 28 days. The physical properties of film thickness, flow, radiopacity, and solubility were evaluated using ISO 6876:2012 standards. RESULTS: All 3 sealers contained calcium, zirconium, and silicon. Totalfill BC had the highest calcium release at 7 and 28 days followed by AH Plus Bioceramic and BC Universal sealer. All 3 sealers adhered to the ISO standard in terms of flow and radiopacity. BC Universal sealer was slightly over the range (>50 µm) for film thickness. All sealers exceeded the solubility range set by ISO 6876:2012. CONCLUSION: Although these hydraulic cement sealers had similar components and delivery, the properties varied significantly. The testing of material properties to confirm the suitability for clinical use is necessary.

Adhesive Performance Assessment of Universal Adhesives and Universal Adhesive/Composite Cement Combinations.

PURPOSE: To investigate the bonding performance of three universal adhesives (UAs) to dentin and the effect of different curing modes and hydrofluoric-acid (HF) etching of lithium-disilicate glass-ceramic on the adhesive performance of two UA/composite cement (CC) combinations. MATERIALS AND METHODS: In the first project part, the immediate and aged (25k and 50k thermocycles) microtensile bond strength (µTBS) of the two light-curing UAs G2-Bond Universal (G2B; GC) and Scotchbond Universal Plus (SBUp; 3M Oral Care), and the self-curing UA Tokuyama Universal Bond II (TUBII; Tokuyama) to flat dentin was measured, when applied in both E&R and SE bonding mode using a split-tooth design (n = 10). The resultant adhesive-dentin interfaces were characterized using TEM. In the second project part, CAD/CAM composite blocks were luted to flat dentin with either Scotchbond Universal Plus/RelyX Universal (SBUp/RxU; 3M Oral Care) or Tokuyama Universal Bond II/Estecem II Plus (TUBII/ECIIp; Tokuyama Dental) using different curing modes (AA mode: auto-curing of both adhesive and cement; AL mode: auto-curing of adhesive and light-curing of cement), upon which their immediate and aged (25k and 50k thermocycles) µTBS was measured. In the third project part, the same UA/CC combinations were luted to CAD/CAM glass-ceramic to measure their immediate and aged (6-month water storage) shear bond strength (SBS). RESULTS: In E&R bonding mode, the performance of G2B, SBUp and TUBII was not significantly different in terms of µTBS, while G2B and SBUp significantly outperformed TUBII in SE bonding mode. No significant difference in µTBS was found between the SBUp/RxU and TUBII/ECIIp UA/CC combinations, regardless of bonding mode, aging time, or curing mode. The cement-curing mode did not significantly influence µTBS, while a significantly higher µTBS was recorded for the UA/CC combinations applied in E&R bonding mode. HF significantly improved the SBS of the UA/CC combinations to glass-ceramic. CONCLUSION: The self-curing adhesive performed better when applied in E&R than in SE bonding mode. The curing mode did not influence the adhesive performance of the composite cements, while an E&R bonding mode rendered more favorable adhesion in a self-curing luting protocol. When bonding to glass-ceramic, the adhesive performance of the universal adhesive/composite cement combinations benefited from HF etching.

In vitro assessment of the effect of luting agents, abutment height, and fatigue on the retention of zirconia crowns luted to titanium base implant abutments.

STATEMENT OF PROBLEM: The bonding of implant-supported prostheses is determined by abutment material, convergence angle, height, surface treatment, and luting agents. However, studies evaluating the bonding of luting agents to titanium base abutments with different heights under fatigue conditions are scarce. PURPOSE: The purpose of this in vitro study was to evaluate the retention of zirconia crowns bonded with different luting agents to titanium base abutments of different heights before and after fatigue testing. MATERIAL AND METHODS: Zirconia crowns were designed, milled, and distributed into 4 experimental groups according to the luting agents (G-Multi Primer/G-Cem LinkForce [MP/GC] and Scotchbond Universal/RelyX Ultimate [SU/RU]) and titanium base abutment heights (2.5 mm and 4 mm) (n=10). Pull-out testing was performed in a universal testing machine at a crosshead speed of 1 mm/min until crown displacement. Fatigue testing was performed by an electric precision fatigue simulator (1×106 cycles; 100 N; and 15 Hz), followed by pull-out testing of fatigued specimens. Collected data were statistically evaluated by using a linear mixed model after post hoc comparisons by the least significant difference test (α=.05). RESULTS: Luting agents, abutment heights, and fatigue influenced the bonding retention of zirconia crowns to titanium base abutments. SU/RU agents promoted higher pull-out compared with MP/GC for both abutment heights before and after fatigue. Higher abutment height increased pull-out regarding lower abutment height for SU/RU materials before and after fatigue testing. Although fatigue had no significant effect on the pull-out of MP/GC, lower bond retention was observed for SU/RU after fatigue, regardless of abutment height. CONCLUSIONS: Luting agent composition and the interaction with abutment height and fatigue influenced the retention of zirconia crowns to titanium base abutments.

Dental and composite resin discoloration induced by different hydraulic calcium silicate-based cements: two-year in vitro assessment.

Few long-term studies assess the discoloration induced by hydraulic calcium silicate-based cement on dental structures. In addition, as far as we know, no long-term study has assessed the discoloration induced by these cement on composite resin. This in vitro study aimed to assess, during a period of two years, the discoloration potential of different hydraulic calcium silicate-based cements (hCSCs) on the enamel/dentin structure and composite resin restoration. A total of 40 enamel/dentin discs were obtained from bovine incisors, and 40 composite resin discs (10 mm in diameter × 2 mm thick) were fabricated. A 0.8 mm-deep cavity was made in the center of each disc and filled with the following hCSCs (n=10): Original MTA (Angelus); MTA Repair HP (Angelus); NeoMTA Plus (Avalon); and Biodentine (Septodont). An initial color measurement was performed (T0 - baseline). After 7, 15, 30, 45, 90, 300 days, and two years, new color measurements were performed to determine the color (ΔE00), lightness (ΔL'), chroma (ΔC'), hue differences (ΔH'), and whiteness index (WID). For enamel/dentin, the ΔE00 was significant among groups and periods (p<0.05). NeoMTA Plus had the greatest ΔE00. The NeoMTA Plus group had the greatest ΔE00 after two years for composite resin. Significant reduction in lightness was observed for all groups after two years (p<0.05). The most significant WID values were observed after 30 days for Biodentine (enamel/dentin) and MTA Repair HP groups (composite resin) (p<0.05). The hCSCs changed the colorimetric behavior of both substrates, leading to greater darkening over time. The Bi2O3 in the Original MTA seems relevant in the short periods of color change assessment.

An assessment of the impact of adhesive coverage and wire type on fixed retainer failures and force propagation along two types of orthodontic retainer wires: an in vitro study.

OBJECTIVES: To evaluate the force required to promote the failure of fixed orthodontic retainers with different adhesive (composite) coverage and to assess the presence and extent of force propagation with two different orthodontic retainer wires. MATERIALS AND METHODS: Ortho-FlexTech and Ortho-Care Perform (0.0175 inches), each of 15-cm length, were bonded on acrylic blocks with different adhesive surface diameters (2 mm, 3 mm, 4 mm, and 5 mm). The samples (n = 160) were subjected to a tensile pull-out test, and debonding force was recorded. Fixed retainers using two different wires and 4-mm adhesive diameter were bonded on acrylic bases resembling a maxillary dental arch (n = 72). The retainers were loaded occluso-apically until the first sign of failure while being video recorded. Individual frames of the recordings were extracted and compared. A force propagation scoring index was developed to quantify the extent of force transmission under load. RESULTS: A 4-mm adhesive surface diameter required the highest debonding force for both retainer wires with significant differences compared with 2 mm (P < .001; 95% confidence interval [CI]: 8.69, 21.69) and 3 mm (P = .026; 95% CI: 0.60, 13.59). Force propagation scores were significantly higher for Ortho-Care Perform. CONCLUSIONS: Based on this laboratory-based assessment, consideration should be given to the fabrication of maxillary fixed retainers using a minimum of 4-mm diameter composite coverage on each tooth. Force appeared to propagate more readily with Ortho-Care Perform than with a flexible chain alternative. This may risk stress accumulation at the terminal ends with potential for associated unwanted tooth movement in the presence of intact fixed retainers.

Assessment of the effect of spacer material on gap and void formation in an endodontic temporary restoration using micro-computed tomography.

This study aimed to evaluate the effect of two spacer materials (cotton pellet and polytetrafluoroethylene [PTFE]) on gap and void formation in the Cavit restoration used for endodontic temporization. Thirty-four extracted human single-rooted premolars were matched and allocated into two groups (n = 17/group) according to the access cavity spacer (cotton pellet or PTFE). Standardized endodontic access cavities were prepared in all the teeth. Subsequently, the teeth were scanned by micro-computed tomography (micro-CT) to determine the volume of the access cavities. The teeth were then temporized by placing either cotton pellet or PTFE as spacer materials on the canal orifices, followed by the Cavit restoration. Following the temporization procedure, the teeth were subjected to a second micro-CT scan to determine the percentage volume of gaps and voids along the margins and within the Cavit restoration, respectively. Statistical analysis was performed using the Shapiro-Wilk and Wilcoxon signed-rank tests with a 5% significance level. The PTFE spacer was associated with significantly less gap formation between the Cavit restoration and the access cavity walls (P < 0.05) compared with the cotton pellet. No difference existed in void formation between the groups (P > 0.05). These findings indicate that the spacer material placed under the Cavit restoration can influence the quality of overlying restoration. PTFE was associated with less gap formation and, therefore, performed better than the cotton pellet as a spacer material beneath the Cavit restoration during endodontic treatment.

Microshear Bond Strength, Ultramorphological, and Elemental Assessment of Gold-Silver Nanoparticle-treated Dentin Bonded to Resin Composite with Different Adhesive Modes.

AIM: The aim of the present study is to evaluate the effect of Ag-Au NPs formulation as surface pretreatment by assessing the microshear bond strength, ultra-morphological, and elemental characteristics of gold-silver nanoparticle-treated dentin to resin composite with different adhesive modes. MATERIALS AND METHODS: A total of 60 extracted sound human permanent molars were selected to test the microshear bond strength of composite resin to dentin (40 teeth) and 20 molars were used to assess the ultramorphological and elemental characteristics of silver-gold nanoparticles using environmental scanning electron microscope (ESEM) with energy-dispersive X-ray (EDX) analyzer. The specimens were randomly divided into two main groups according to measured tests either microshear bond strength or elemental analysis. For the bond-strength testing group, 40 teeth were equally distributed into two main groups (20 each) according to dentin-surface pretreatment with or without the use of silver-gold nanoparticles. Then, each group was further divided into two subgroups (10 specimens for each) according to adhesive mode. For ultramorphological and elemental analysis, 20 teeth were equally divided into similar previously mentioned groups according to treatment modalities (5 teeth per group). The multimode bonding agent used in this study was single-bond universal, 3M. The data were collected and statistically analyzed. The significance level was set at p ≤0.05. RESULTS: The results showed that different etching modes had no statistically significant effect. Also, pretreatment had no statistically significant effect. The interaction between the two variables also had no statistically significant effect. Majority of samples in all groups had mixed failure mode. Ultramorphological examination of the tested samples treated with silver-gold nanoparticles revealed proper dispersion of nanoparticles in dentin. CONCLUSIONS: The new formulation of silver-gold nanoparticles did not interfere with the bond strength and sealing ability of resin composite restoration in different adhesion modes. CLINICAL SIGNIFICANCE: The cavity disinfection with silver-gold nanoparticles did not affect the marginal integrity of resin composite restoration. Further studies should be done to evaluate the impact of application of silver-gold nanoparticles in long duration with other adhesive systems in the clinical scenario.

Reliability of qualitative and quantitative assessment of adhesive remnants after debonding of ceramic brackets bonded with Transbond™XT on human molar teeth: An in vitro study.

OBJECTIVE: To test the hypothesis that there are any differences between qualitative and quantitative methods of adhesive remnants index (ARI) scores on the buccal surfaces of the upper molar. MATERIALS AND METHODS: The study included 34 upper first human molars. After etching for 20seconds by using 35% orthophosphoric acid (Ultra-Etch, Ultradent Products Inc., USA) and then rinsed with distilled water for 15seconds, the adhesive (Transbond™XT, 3M™, USA) was applied and cured with the polymerization lamp (Bluephase Style, Ivoclar Vivadent, Liechtenstein) for 10seconds. Ceramic brackets (Damon® Clear™, Ormco, USA) were placed by composite (Transbond™XT, 3M™, USA) on the buccal surface of the molars. Following 24-hour storage of the samples in distilled water (WB-12, Phoenix Instrument, Gabsen, Germany) at 37°C, the brackets were debonded using a debonding plier (Damon Clear Debonding Plier, Ormco, USA). ARI and a modified ARI-scoring system were used. Three independent observers scored the ARI with the naked eye under 10x, 20x, and 32x magnifications using Stereomicroscope Stemi DV4 (Zeiss, Germany). Afterward, the digital microscope VHX-5000 (Keyence, Japan) under 50x magnification was used for the quantitative analysis of the adhesive remnants by just one evaluator. The Fleiss' Kappa-coefficient test was used to assess interrupter reliability. RESULTS: Fleiss' Kappa-coefficient test show that the highest amount of agreement between observers is observed using the naked eye for both of the scoring methods P<0.001. Increasing the magnification led to the moderate decrease of inter-observer agreement. For evaluations with naked eye, a higher agreement is observed for the modified ARI score compared to the ARI scored. The agreement between the quantitative and qualitative evaluation of the ARI score rose with increasing magnification. CONCLUSION: The assessment of adhesive remnants should be done either with naked eye or under 10x magnification, as they showed the highest rate of intra-observer agreement.

Assessment of chemical, ultrasonic, diode laser, and Er:YAG laser application on debonding of ceramic brackets.

BACKGROUND: Risk of enamel damage that often accompanies ceramic brackets debonding raises the demand of finding an optimal method for debonding of them without adverse effects. Different techniques were proposed in an attempt to facilitate their debonding. Comparison of these techniques is crucial. The aim of this study was to evaluate and compare different techniques for debonding of ceramic brackets in terms of shear bond strength and adhesive remnant index. MATERIALS AND METHODS: A total of 100 extracted premolars were randomly allocated into 5 groups. Ceramic brackets were then bonded to teeth using light cure composite resin. Among test groups; group I: served as control, group II: chemical aided debonding via peppermint oil, group III: ultrasonic aided debonding, group IV: diode laser aided debonding, and group V: Er:YAG laser aided debonding. Brackets were shear tested using universal testing machine followed by ARI assessment and evaluation of enamel microstructure was performed using scanning electron microscopy. RESULTS: A significantly lower shear bond strength was found in ultrasonic, diode, and Er:YAG laser groups. However, no significant difference was found in the chemical group. A significantly higher adhesive remnant index was found solely in Er:YAG laser group with minimal enamel microstructure alterations. CONCLUSIONS: Er:YAG laser is a promising tool in debonding ceramic brackets. Ultrasonic and diode laser significantly reduced shear bond strength. Yet, adhesive remnant index in both groups revealed no difference. Chemical aided debonding had little effect and hence, it cannot be recommended without further development.

Clinical and OCT assessment of application modes of a universal adhesive in a 12-month RCT.

OBJECTIVES: To evaluate a universal adhesive clinically using FDI criteria and by optical coherence tomography (OCT). METHODS: In 50 patients, three or four non-carious cervical lesions (NCCL) were restored with composite (Venus® Diamond Flow, Kulzer) using iBond® Universal (iBU, Kulzer) applied in self-etch (iBU-SE, n = 50), selective-enamel-etch (iBU-SEE, n = 29) or etch-and-rinse mode (iBU-ER, n = 50) and the reference OptiBond™ FL (OFL, Kerr, n = 50). Restorations were imaged by SD-OCT. The weighted mean length of interfacial adhesive defects (AD, %) was quantified per restoration immediately after placement (t0), simultaneously with clinical assessment (FDI criteria) after 14 days (t1), 6 (t2) and 12 months (t3). Data were statistically analyzed (McNemar-/Wilcoxon-/Mann-Whitney-U test (α = 0.05), Kaplan-Meier survival curves). RESULTS: After 12 months, cumulative failure rates were lower with iBU-SE (0.0%; p = 0.016), iBU-SEE (0.0%; p = 0.125), and iBU-ER (2.1%; p = 0.070; loss t3) compared to OFL (16.7%; losses t2, t3). Generally, marginal adaptation decreased (pi < 0.001) and marginal staining increased (pi ≤ 0.031), without significant group differences (pi > 0.064). AD increased in all groups (pi < 0.001). At enamel, AD appeared more extended with iBU-SE vs. iBU-SEE (t2-t3; pi ≤ 0.005), iBU-ER (t1-t3; pi ≤ 0.051) and OFL (t0-t3; pi ≤ 0.018). At dentin/cement iBU generally caused fewer defects than OFL (t1-t3; pi ≤ 0.010) and with SE vs. ER (t2-t3; pi = 0.010). CONCLUSIONS: In NCCLs, iBU generally provides a more durable bond than OFL. Recommended mode is SEE. Clinic and OCT provided comparable results. OCT has higher statistical power, shows group differences earlier and specifically for the different hard tooth tissues. CLINICAL SIGNIFICANCE: The universal adhesive iBU was superior against the reference OFL in NCCLs. It can be recommended with SEE. Evaluation of interfacial adhesive defects by OCT seems to allow early prediction of adhesives' clinical performance.

Impact of economic factors and knowledge translation on public procurement for dental adhesive systems.

This study aimed to screen the public procurement for dental adhesives in a public health system and evaluate the variables associated with the type and cost of purchased materials. A time series was conducted with secondary data collated from the Brazilian Databank of Healthcare Prices (BDHP). All public procurements for adhesive systems registered from 2010 to 2019 were collated. The frequency of purchase was described, and a multiple linear regression model was used to assess the influence of unit price on predictor variables. Multinomial logistic regression was conducted to describe the influence of selected variables on the probability of purchase for different classes of adhesive systems. A total of 2,752 purchases were analyzed, and the total cost of these materials reached US$ 7,022,659.00 in the 10 analyzed years. The 3-step Etch & Rinse adhesives corresponded to 45.63% of the processes, with increased probability of being purchased over time. The process conducted by public universities in recent years, using the auction modality, presented lower unit prices. The year of purchase, the type of institutions, the institution's location, and the procurement modality influenced the procurement process. The performed analyses contribute to the understanding of the variables associated with the procurements for adhesive systems, providing valuable information for rationalizing public expenditure and for implementing evidence-based practices to guarantee cost-effective and clinically effective procedures for users of the Brazilian public healthcare system.

Physicochemical and microbiological assessment of a dental adhesive doped with cashew nut shell liquid.

To evaluate i) the inhibitory and bactericidal activity of cashew nut shell liquid (CNSL) and its isolated compounds (anacardic acid and cardol) against oral bacteria; ii) the biofilm formation inhibition, resin-dentin bond strength and physicochemical properties of a dental adhesive incorporated with these substances. The antibacterial effect of CNSL, anacardic acid, and cardol were assessed by determining the minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations. Effect in inhibiting biofilm formation of the adhesive incorporated with the substances (15 µg/ml) against a mixed-species biofilm of Streptococcus mutans and Candida Albicans and was determined by direct contact test. Additional Analysis included microtensile bond strength (µTBS) test, elastic modulus (EM), flexural strength (FS), degree of conversion (DC), water sorption (WS) and solubility (SL). The data were submitted to statistical analysis by one-way ANOVA and Tukey's test (p < 0.05). CNSL, anacardic acid and cardol showed antibacterial activity for all strains tested, with MIC and MBC values ranging from 3.12 to 25 µg/ml. There was no growth of colonies forming units in the adhesives incorporated with the substances. EM increased in the adhesive incorporated with anacardic acid, decreased after incorporation of cardol and it was not affected by incorporation of CNSL. The substances tested showed no effect in FS, DC, WS, SL and µTBS. In conclusion, the CNSL, anacardic acid and cardol showed antibacterial effects against oral bacteria and, the incorporation of substances did not reduce the performance of the adhesive.

Assessment of bacterial and dye penetration through post/crown interim restorations while under simulated masticatory load.

The aim was to compare two temporary cements to determine which resisted bacterial and dye penetration under temporary posts/cores/crowns subjected to simulated masticatory function. Forty-six single canal human tooth roots were prepared for posts/crowns. A cotton pellet and Cavit were placed in each post space. Temporary posts/cores/crowns were cemented with Intermediate Restorative Material (IRM) (n = 23) or Tempocem (n = 23). Specimens were sterilised, then immersed in a mixture of Streptococcus gordonii and India ink, and subjected to 4 weeks simulated mastication. Dye penetration was assessed by visually inspecting the cotton pellets. Bacterial penetration was determined by placing the pellets into sterile broth and by plating them onto agar plates to confirm S. gordonii growth. There was no statistically significant difference between the cements and the dependent variables of bacterial and dye penetration. Hence, both cements are good options to cement temporary posts/cores/crowns during endodontic treatment of anterior teeth.

Assessment of Microleakage under Stainless Steel Orthodontic Brackets Bonded with Various Adhesive Systems: An In Vitro Study.

AIM AND OBJECTIVE: The aim of this study was to evaluate the extent of microleakage beneath stainless steel orthodontic brackets bonded with different adhesive systems. MATERIALS AND METHODS: Freshly extracted 60 human premolar teeth from mandibular arch were included in this study. After sterilizing all teeth, they were stored in thymol solution of 1% for further preparation. Acrylic blocks were used to mount the teeth in a way their roots were totally implanted up to the cement enamel junction in acrylic with crown being visible. A 0.022 slot, stainless steel preadjusted edgewise premolar brackets were taken. Sixty premolars were categorized randomly into three groups (20 premolars in each group) as follows: group I: flowable composite, group II: Fuji Ortho LC, group III: Transbond XT. Later, all the samples were subjected to thermocycling and tested immediately and 24 hours after water storage. The samples were submerged for 24 hours in methylene blue solution (2%) at room temperature. A ×20 magnification stereomicroscope was used to examine all samples. RESULTS: The lowest microleakage (1.34 ± 0.20) was shown by Transbond XT restored teeth, followed by flowable composite group (1.79 ± 0.32) and Fuji Ortho LC group (2.98 ± 0.13). An analysis of variance showed statistically significant differences among various adhesive systems. A statistically significant difference (p <0.05) among groups I and II, and groups II and III adhesive materials was seen. CONCLUSION: This study demonstrated microleakage in all the examined adhesive groups but the lowest microleakage was found with Transbond XT group followed next by Filtek Z350 XT group and Fuji Ortho LC group. CLINICAL SIGNIFICANCE: Due to microleakage, the bacteria and fluids present intraorally penetrate through the gaps along the enamel-adhesive boundary. This penetration results in significant esthetic and clinical complications. Such problems related to microleakage can be addressed with the use of an appropriate adhesive agent.

Effects of non-thermal atmospheric plasma on dentin wetting and adhesive bonding efficiency: Systematic review and meta-analysis.

OBJECTIVES: This systematic review and meta-analysis evaluated the effects of non-thermal atmospheric plasma (NTAP) treatment on dentin wetting and adhesive-dentin bond strength. DATA/SOURCES: This report followed the PRISMA 2020 statement. Two reviewers conducted literature search of MEDLINE, Web of Science and Scopus databases up to the end of November 2020. Included in vitro studies compared the effect of NTAP on treated dentin of non-carious, intact, extracted human third molar teeth with different control groups. Studies with no adequate methods, missing data, lack of control group, or those using animal teeth were excluded. The random effects model was used to summarize the treatment effect with standardized mean difference (SMD) and 95% CI. Risk of bias was assessed using the custom Cochrane Collaboration's tool. STUDY SELECTION/RESULTS: Seventeen studies met inclusion criteria. The effects on dentin wetting were reported in favor of NTAP (SMD -5.38; 95% CI [-6.97, -3.78]; p<0.00001; I2=81%). Regarding adhesive-dentin bond strength, statistically significant differences between the NTAP and control group were in favor of NTAP in the short-term (SMD 1.92; 95%CI [1.35, 2.50]; p<0.00001; I2=97%), and long-term (SMD 3.28; 95%CI [2.46, 4.09]; p<0.00001; I2=97%). A limitation of meta-analysis is moderate heterogeneity caused by methodological differences and lack of data, which was evaluated through risk of bias and sensitivity analysis. CONCLUSIONS: NTAP substantially improves dentin wetting and adhesive-dentin bond strength with 30 seconds exposure time and up to 10 mm tip-to-surface distances being sufficient for positive NTAP effects on bonding efficiency. FUNDING: ON172207 and III41008 from the Ministry of Education, Science and Technological Development, Republic of Serbia. NP is funded by MESTD grant number 451-03-68/2020-14/200024. CLINICAL SIGNIFICANCE: This systematic review and meta-analysis substantiate potential applicability of NTAP treatment of dentin in improving adhesive bonding clinically. Further research should be based on the optimized parameters such as time and distance with additional refinement of NTAP power.