Accuracy of definitive rapid onsite evaluation cytopathology diagnoses: Assessment of potentially critical diagnoses as a quality assurance measure.

INTRODUCTION: Intraprocedural rapid onsite evaluation (ROSE) of cytology specimens enhances cytopathology practice. More recently, ROSE diagnoses, like frozen section (FS) diagnoses, have guided immediate clinical decisions. In this study, we evaluated the diagnostic accuracy of definitive ROSE diagnoses in our quality assurance system over a 52-month period. MATERIALS AND METHODS: Cytopathology cases with ROSE from January 2017 to April 2021were retrieved from our laboratory information system. After excluding cases that were deferred or nondiagnostic/unsatisfactory, each definitive ROSE diagnosis (ie, negative for malignant cells or positive for malignant cells) was categorized as having agreement or disagreement with the final diagnosis. For comparison, concordance of FS diagnoses from the same time period were tabulated and compared to those of ROSE diagnoses by using χ2 testing with P < 0.05 considered statistically significant. RESULTS: Of the 1649 ROSE diagnoses, there were 15 disagreements (0.9%) with 1 final moderate interpretive disagreement (0.06%). By comparison, of the 17,469 FS diagnoses, there were 141 disagreements (0.8%) with 49 final moderate or major interpretive disagreements (0.3%). The remaining disagreements were minor. There were no statistically significant differences in the rates of final moderate and major interpretive disagreements. CONCLUSIONS: The final interpretive disagreement rates for definitive ROSE and FS diagnoses were similar in this study. Given the expanding role of ROSE and its use for immediate clinical decisions in some cases, monitoring the accuracy of definitive diagnoses may serve as an initial quality assurance measure.

Harmonization of training, training program requirements, board certification, and the practice of cytopathology: data from the American Board of Pathology surveys.

INTRODUCTION: The American Board of Pathology (ABPath) has ongoing efforts to better align certification with graduate medical education, training program requirements, and pathology practice. The present study focused on the subspecialty of cytopathology. We evaluated the current content and scope of fellowship programs, practice patterns and needs of diplomates, and program director (PD) and diplomate perceptions of the ABPath certification examination to identify gaps and provide an evidence base to guide harmonization in these areas. METHODS: Two surveys were administered: one directed to PDs of all 93 Accreditation Council for Graduate Medical Education (ACGME) cytopathology fellowship programs and the other to cytopathology diplomates submitting continuing certification reporting to the ABPath. RESULTS: Most (86%) cytopathology diplomates work in smaller groups. Only 11% do >50% cytopathology in practice. Diplomates' cytopathology-related practice tasks varied, as did their perception of the content of fellowship training aligning with practice needs. In fellowship training programs, the specimen types, volumes, techniques of specimen acquisition, and graduated responsibility varied significantly. We identified areas in which current training and certification requirements are challenging for some programs. Diplomates and PDs had differing perceptions of the cytopathology examination; diplomates regarded image-based and microscopic glass slide questions as the best assessment of their knowledge. CONCLUSIONS: First, fellowship training programs could benefit from shared resources and should provide more graduated responsibility for fellows. Second, the ACGME Review Committee could consider this data in future program requirement revisions. Finally, information from these surveys will be useful as the ABPath adjusts certification examination content and delivery.

Comparison of quantitative internal and external measures of performance for trainees in cytopathology fellowships.

INTRODUCTION: Cytopathology fellowships need measures to assess performance of fellows. We sought to compare several internal quantitative assessment metrics in our fellowship with external metrics, such as performance on the American Society of Cytopathology (ASC) Progressive Evaluation of Competency (PEC) examination and United States Medical Licensing Examination (USMLE). METHODS: Quantitative parameters generated from our laboratory information system (LIS) on cytopathology fellows were evaluated over 6 years, including case volume and diagnostic discrepancies, in addition to ASC PEC and USMLE scores. For discrepancy reports, interpretations made by the fellow were compared with that of the cytopathologist, and classified as none (concordant), minor (<2-levels) or major (≥2-levels). RESULTS: We evaluated internal and external metrics on 13 fellows over 6 years. The program average diagnostic concordance rate was 89.9%, with an average major discrepancy rate of 1.5%, and an average monthly case volume of 260 cases. More fellows with above-average ASC PEC performance showed above-average concordant diagnoses and lower case volume, while below-average PEC scores were seen more often with higher major discrepancy rates. More fellows with above-average USMLE scores had higher case volumes, while low USMLE scores showed a trend towards higher major discrepancy rates. CONCLUSION: Our fellowship program has used a variety of internal and external measures of performance for cytopathology fellows. Although the findings show no statistically significant finding correlating performance, these quantitative parameters generated from our LIS were helpful to identify areas of improvement, facilitate comparison to peers, and provide case volume documentation.

Trends in cytopathology fellowship positions and vacancies over the past decade.

INTRODUCTION: Cytopathology is one of the most sought-after fellowships within pathology, with a lower fellowship vacancy rate compared with most other subspecialties. The Accreditation Council for Graduate Medical Education (ACGME) actively tracks annual program data for cytopathology fellowship programs, and evaluating this longitudinal data looking at trends in programs and positions over the past 10 years could provide insights into the future of cytopathology and its training programs. METHODS: Data obtained from the ACGME was examined in detail for all ACGME-accredited cytopathology fellowship programs over the past decade (2011-2021). Additional responses from program directors (PDs) from a 2021 American Society of Cytopathology (ASC) survey are also included. RESULTS: The total number of ACGME-approved cytopathology training programs and cytopathology fellowship positions remained relatively constant over the past 10 years, but the vacancy rate and number of programs with 1-2 unfilled spots has gradually but steadily risen over the past 6 years. In a 2021 ASC PD survey with 66% response rate, 53% of PDs reported having recruitment problems at least occasionally and 46% reported an increase in unexpected fellowship openings. CONCLUSIONS: Although the number of cytopathology positions has been relatively constant over the past decade, there has been a recent increase in cytopathology fellowship vacancies that may indicate changes in career choices or the job market, with fellows choosing jobs over additional fellowships, and potentially signal a growing shortage of fellowship-trained, Board-certified cytopathologists in the coming years.

Cytopathology fellowship recruitment: Has the time come to consider a unified approach?

INTRODUCTION: Cytopathology (CYP) fellowship training is a critical component of maintaining a skilled group of cytopathologists. For years, the recruitment process for CYP fellowship programs has remained unchanged, with individual programs outlining their own requirements and timeline, and applicants bearing the cost of travel and dealing with the variable processes outlined by individual programs. However, there has been renewed interest in analyzing the recruitment process for CYP fellowships to look for areas of potential improvement and uniformity. METHODS: With the goal of gauging the interest of CYP fellowship program directors (PDs) in a more unified approach to recruitment or a formal match process, the ASC Cytopathology Program Directors Committee (CPDC) surveyed PDs via SurveyMonkey and organized special webinars with polling over a 4-year time frame (2017-2021), and examined Qualtrics survey data collected by the American Board of Pathology (ABPath) in 2020. RESULTS: The response rate for PDs was greatest in a formal survey by the ABPath (66 respondents; 71% of PDs) conducted in 2020, and lower for an ASC survey in 2021 (61 respondents, 66% of PDs) and 2017 (19 respondents; 21% of PDs) and two recent ASC webinars (10 and 26 respondents; 11% and 28% of PDs). Support for a fellowship match process varied from 29% to 77%, respondent uncertainty ranged from 13% to 50%, and a lack of support ranged from 10% to 60%. In aggregate, approximately 56% of respondents would be in favor of a more standardized process. Recently, after hearing about other fellowships experimenting with a standardized process, the interest in a unified approach doubled from approximately 29% to 60%, and the percentage of PDs with uncertainty decreased from 50% to 26%. In the most recent follow up survey, interest reached the highest level of 77% among PDs. CONCLUSIONS: Herein we present several years of feedback from the CYP fellowship PD community regarding a more standardized approach to CYP fellowship recruitment, culminating in the latest survey with 77% of CYP fellowship PDs expressing interest. Thus, details about what a unified timeframe may look like for CYP fellowships is presented to show how this may improve the recruitment process for the mutual benefit for programs and applicants.

A human factor event-based learning assessment tool for assessment of errors and diagnostic accuracy in histopathology and cytopathology.

This review article summarises systems for categorisation of diagnostic errors in pathology and cytology with regard to diagnostic accuracy and the published information on human factors (HFs) in pathology to date. A 12-point event-based checklist for errors of diagnostic accuracy in histopathology and cytopathology is proposed derived from Dupont's 'Dirty Dozen' HF checklist, as used in the aerospace industry for aircraft maintenance. This HF checklist comprises 12 HFs; (1) Failure of communication. (2) Complacency. (3) Lack of knowledge. (4) Distractions. (5) Lack of teamwork. (6) Fatigue. (7) Lack of resources. (8) Pressure. (9) Lack of assertiveness. (10) Stress. (11) Norms. (12) Lack of awareness. The accompanying article explains practical examples of how each of these 12 HFs may cause errors in diagnostic accuracy in pathology. This checklist could be used as a template for analysis of accuracy and risk of diagnostic error in pathology either retrospectively 'after the event' or prospectively at the time of diagnosis. There is a need for further evaluation and validation of this proposed 12-point HF checklist and similar systems for categorisation of diagnostic errors and diagnostic accuracy in pathology based on HF principles.

Ernest Everett Just (1883-1941): Hero in Cell Biology and Evolutionary Bioethics.

Ernest Everett Just is celebrated for his contributions to cell biology. Among other firsts, he was first to describe the "wave of negativity" spreading around an egg cell from the entrance point of the fertilizing spermatozoon. His accomplishments in biology are celebrated in Black Apollo of Science (1983) by Kenneth Manning, and by a 1996 Black Heritage postage stamp. What is not yet widely appreciated, however, is that Just connected evolutionary biology to ethical behavior (1933, 1939, 1940). He was probably the first cell biologist to argue that human ethical behavior evolved from our very most primitive cellular origins. Today, Just's contributions to evolutionary bioethics, including "the law of environmental dependence," can be better appreciated because his unpublished booklength manuscript, "The Origin of Man's Ethical Behavior" has been preserved at Howard University's Moorland-Spingarn Research Center.

Lillian Fritz-Laylin: Keeping up to speed with evolutionary cell biology.

Fritz-Laylin studies the evolution of complex protein networks associated with cell movement.

Summary of the National Institute of Standards and Technology and US Food And Drug Administration cell counting workshop: Sharing practices in cell counting measurements.

The emergence of cell-based therapeutics has increased the need for high-quality, robust and validated measurements for cell characterization. Cell count, being one of the most fundamental measures for cell-based therapeutics, now requires increased levels of measurement confidence. The National Institute of Standards and Technology (NIST) and the US Food and Drug Administration (FDA) jointly hosted a workshop focused on cell counting in April 2017 entitled "NIST-FDA Cell Counting Workshop: Sharing Practices in Cell Counting Measurements." The focus of the workshop was on approaches for selecting, designing and validating cell counting methods and overcoming gaps in obtaining sufficient measurement assurance for cell counting. Key workshop discussion points, representing approximately 50 subject matter experts from industry, academia and government agencies, are summarized here. A key conclusion is the need to design the most appropriate cell counting method, including control/measurement assurance strategies, for a specific counting purposes. There remains a need for documentary standards for streamlining the process to develop, qualify and validate cell counting measurements as well as community-driven efforts to develop new or improved biological and non-biological reference materials.

Markov Decision Process Measurement Model.

Within-task actions can provide additional information on student competencies but are challenging to model. This paper explores the potential of using a cognitive model for decision making, the Markov decision process, to provide a mapping between within-task actions and latent traits of interest. Psychometric properties of the model are explored, and simulation studies report on parameter recovery within the context of a simple strategy game. The model is then applied to empirical data from an educational game. Estimates from the model are found to correlate more strongly with posttest results than a partial-credit IRT model based on outcome data alone.

Manejo de los hallazgos anormales prevalentes en reportes de citología cervico vaginal de usuarias de 20 a 59 años de la Unidad Comunitaria de Salud Familiar Intermedia San Rafael Cedros Cuscatlán, Enero a Junio 2018 / Management of prevalent abnormal findings in cervical-vaginal cytology reports of users from 20 to 59 years of the San Rafael Cedros Community Intermediate Family Health Unit, Cuscatlán, January to June 2018

Propósito: que los resultados sirvan para reforzar: 1) El programa de prevención y control del cáncer cervicouterino, 2) El cumplimiento de manejo de resultados de citología cervico vaginal de acuerdo a lineamientos técnicos vigentes del Ministerio de Salud y además sirva de referente para futuras investigaciones. Materiales y Métodos: investigación descriptiva con enfoque cuantitativo, de corte transversal. Resultados: los tres hallazgos anormales prevalentes en una muestra de 161 usuarias fueron en primer lugar los cambios celulares reactivos asociados a inflamación (inflamación) con un 62.1%, los otros dos hallazgos fueron lesiones intraepiteliales con un 10%, de este las lesiones intraepiteliales de bajo grado LEI BG representaban el 7.5% y las lesiones intraepiteliales de alto grado LEI AG un 2.5%, entre las características de las usuarias con estos hallazgos se encontró que la mayoría tenia de 20 a 29 años, eran del área rural, multíparas, no hacían uso de métodos anticonceptivos y se realizaban toma de citología con la periodicidad establecida, en cuanto al manejo de resultados de las usuarias con Lesiones intraepiteliales el 100% fue manejado de acuerdo a lineamientos y en la inflamación en el 78% de los casos no se encontró evidencia escrita en expediente clínico de respuesta y manejo. Conclusiones: en casi tres cuartas partes de las usuarias en estudio se verificaron hallazgos anormales en los reportes de citología, a la gran mayoría de usuarias con inflamación no se le brindo el manejo de acuerdo a lineamientos técnicos vigentes. Recomendaciones: mejoras y supervisiones constantes al programa de prevención y control de cáncer cervico uterino en la institución

Primary HPV testing versus cytology-based cervical screening in women in Australia vaccinated for HPV and unvaccinated: effectiveness and economic assessment for the National Cervical Screening Program.

BACKGROUND: Australia's National Cervical Screening Program currently recommends cytological screening every 2 years for women aged 18-69 years. Human papillomavirus (HPV) vaccination was implemented in 2007 with high population coverage, and falls in high-grade lesions in young women have been reported extensively. This decline prompted a major review of the National Cervical Screening Program and new clinical management guidelines, for which we undertook this analysis. METHODS: We did effectiveness modelling and an economic assessment of potential new screening strategies, using a model of HPV transmission, vaccination, natural history, and cervical screening. First, we evaluated 132 screening strategies, including those based on cytology and primary HPV testing. Second, after a recommendation was made to adopt primary HPV screening with partial genotyping and direct referral to colposcopy of women positive for HPV16/18, we evaluated the final effect of HPV screening after incorporating new clinical guidelines for women positive for HPV. Both evaluations considered both unvaccinated and vaccinated cohorts. FINDINGS: Strategies entailing HPV testing every 5 years and either partial genotyping for HPV16/18 or cytological co-testing were the most effective. One of the most effective and cost-effective strategies comprised primary HPV screening with referral of women positive for oncogenic HPV16/18 direct to colposcopy, with reflex cytological triage for women with other oncogenic types and direct referral for those in this group with high-grade cytological findings. After incorporating detailed clinical guidelines recommendations, this strategy is predicted to reduce cervical cancer incidence and mortality by 31% and 36%, respectively, in unvaccinated cohorts, and by 24% and 29%, respectively, in cohorts offered vaccination. Furthermore, this strategy is predicted to reduce costs by up to 19% for unvaccinated cohorts and 26% for cohorts offered vaccination, compared with the current programme. INTERPRETATION: Primary HPV screening every 5 years with partial genotyping is predicted to be substantially more effective and potentially cost-saving compared with the current cytology-based screening programme undertaken every 2 years. These findings underpin the decision to transition to primary HPV screening with partial genotyping in the Australian National Cervical Screening Program, which will occur in May, 2017. FUNDING: Department of Health, Australia.

A importância da descrição ultrassonográfica padronizada e da punção aspirativa por agulha fina na avaliação de nódulos tireoidianos / The importance of a standardized ultrasonographic description and fine-needle aspiration biopsy in the evaluation of thyroid nodules

Nódulos tireoidianos são muito frequentes, sendo detectáveis pela palpação em cerca de 5% das mulheres. A ultrassonografia e a punção aspirativa por agulha fina (PAAF) são métodos diagnósticos largamente utilizados na propedêutica da doença nodular da tireoide. O objetivo deste estudo foi descrever a utilização da PAAF em pacientes com nódulos tireoidianos submetidos à tireoidectomia. Foram incluídos 20 pacientes, com média de idade de 46±14 anos, (02 homens/18 mulheres), submetidos à PAAF e tireoidectomia em um hospital universitário, entre janeiro/2010 e abril/2016. Apenas 12,5% dos laudos ultrassonográficos descreveram o tamanho dos nódulos em três dimensões e nenhum deles continha todos os itens recomendados para caracterização adequada de um nódulo tireoidiano. De um total de 32 nódulos estudados, 8% constituíram amostra não diagnóstica (categoria Bethesda I). Após tireoidectomia, seis (28%) pacientes apresentaram carcinoma papilífero, sendo dois (33,3%) classificados como Bethesda III e quatro (66,6%) Bethesda V e VI. Observou-se que a falta de padronização dos laudos ultrassonográficos de tireoide pode retardar a investigação diagnóstica e gerar custos elevados para o sistema de saúde. Em contrapartida, o sistema Bethesda está bem consolidado como uma ferramenta para direcionar a conduta frente à doença nodular de tireoide.

Thyroid nodules are very common and can be detected by palpation in about 5% of women. Ultrasonography and fine-needle aspiration biopsy (FNAB) are diagnostic methods widely used in the management of nodular thyroid disease. The aim of this study was to describe the experience on using FNAB in patients with thyroid nodules submitted to thyroidectomy. The sample included 20 patients with mean age of 46±14 years (2 male/18 female), who underwent both FNAB and thyroidectomy at a university hospital between January 2010 and April 2016. Only 12.5% of the ultrasound reports have described the size of the nodules in three dimensions and none of them has described all the characteristics that are necessary to characterize a thyroid nodule properly. A total of 32 evaluated nodules, 8% were nondiagnostic specimens (Bethesda I category). After thyroidectomy, six (28%) patients had papillary carcinoma, two (33.3%) of which were classified as Bethesda III and the other four (66.6%) between Bethesda V or VI. It was observed that the lack of standardization of the thyroid nodule ultrasound reports can delay the diagnostic procedures and generate high costs for the health system. In contrast, the Bethesda system is well established as a tool for guiding the medical management of thyroid nodules.