Assuntos
Anticorpos/genética , Anticorpos/imunologia , Camundongos Transgênicos/imunologia , Animais , Anticorpos/uso terapêutico , Cromossomos Artificiais de Levedura/genética , Ensaios Clínicos como Assunto , Indústria Farmacêutica/economia , Marcação de Genes , Engenharia Genética , Humanos , Imunoglobulinas/genética , Imunoglobulinas/imunologia , Imunoglobulinas/uso terapêutico , Propriedade Intelectual , Camundongos , Camundongos Transgênicos/genética , Biblioteca de Peptídeos , Recombinação Genética/genéticaAssuntos
Sequência de Bases , DNA/química , Reação em Cadeia da Polimerase/métodos , Cromossomos Artificiais de Levedura , Clonagem Molecular/métodos , Custos e Análise de Custo , Primers do DNA , Genes Bacterianos , Humanos , National Institutes of Health (U.S.) , Neoplasias/genética , Reação em Cadeia da Polimerase/economia , Estados UnidosRESUMO
Artificial amplicons of known size, constructed by use of YACs featuring human 8p12 and 12q13, were analyzed by comparative genomic hybridization (CGH). A minimum of 15 Mb of overrepresented DNA sequences could be detected. The sensitivity is (1) not dependent on the chromosome site and (2) related to the size of the amplicon, decreasing with decreasing size.
Assuntos
Mapeamento Cromossômico/métodos , Cromossomos Artificiais de Levedura , Genoma Humano , Hibridização de Ácido Nucleico/métodos , DNA Fúngico/análise , Marcadores Genéticos , Humanos , Sensibilidade e EspecificidadeRESUMO
A mutation in the GIRK2 inwardly rectifying K+ channel was mapped recently to the region of mouse chromosome 16 containing the wv gene and shown to occur in mutant but not in wild-type mice. We demonstrate tight linkage of the Girk2 mutation to the wv phenotype and refine the localization of the weaver (wv) gene on recombinational and physical maps. This linkage between Girk2 and wv has existed since at least 1988 in descendants of the original mutation maintained in C57BL/6 animals. Girk2 is shown to be transcribed in brain before the first recognized manifestation of the wv phenotype and in cultures of granule cells (GCs) isolated from cerebellum at postnatal day 8. Wild-type GCs grown in this culture system display an important developmental property--the ability to extend neurites. However, no inwardly rectifying K+ current is detected in GCs cultured from either wv/wv or +/+ cerebellum under a variety of conditions that activate related channels in other tissues. This suggests that if the Girk2 mutation is responsible for the wv phenotype, it does not act by altering these electrical properties of developing GCs.