Multiple scale assessment of habitat, landscape, and geographic-specific attributes driving decapod assemblages in Posidonia oceanica seagrass meadows.

Seagrass meadows are biodiversity hotspots for invertebrate species including decapods. Understanding the drivers of species abundance, richness and diversity of decapod assemblages is crucial for the conservation of such hotspots, but how drivers act across multiple spatial scales remains unexplored. Here we describe the decapod assemblages of Posidonia oceanica seagrass meadows and assess the influence of attributes from three increasing spatial scales (habitat, landscape, and geographical levels) on the assemblages' structure and composition, as well as the variability partitioning among each one of these levels. Overall, geographical level attributes (i.e., inlet aperture, confinement) affected the most the decapod assemblages, while we only found a modest contribution from habitat (e.g., detritus biomass, sediment organic matter) and landscape attributes (e.g., fragmentation). We suggest that decapod assemblages are driven by the interaction of multiple processes occurring at different scales and other highly stochastic phenomena such as larval dispersion and recruitment.

Trace metals in commercial seafood products (canned, pickled and smoked): Comparison, exposure and health risk assessment.

This study used inductively coupled plasma and mass spectrometry, followed by microwave digestion, to assess the concentration of six trace metals (Cr, Ni, As, Cd, Hg, and Pb) in three canned products (tuna in oil [TIO], sardine in oil [SIO], and mackerel in oil [MIO]), two pickled products (prawn pickle [PP] and fish pickle [FP]), and one smoked product (masmin) collected from Tuticorin market, southern India. Trace metal (TM) levels in canned, pickled, and smoked fish varied from 0.01 to 1.48, 0.009 to 0.94, and 0.08 to 4.13 mg/kg, respectively. The concentration of TMs in the seafood was observed in the following order: As > Cr > Pb > Ni > Cd > Hg. Regarding their accumulation in the seafood products, masmin showed the highest levels followed by SIO, MIO, TIO, FP, and PP. The results revealed that smoked products showed higher TM concentrations, followed by canned and pickled products. The level of TMs in seafood products was below the maximum residue limit set by the European Commission (EC/1881/2006) and the Food Safety and Standards Authority of India (FSSAI). Risk assessment for consumer health implied that the evaluated target hazard quotient (THQ < 1), hazard index (HI < 1), and target cancer risk (TCR < 1.E-04) values did not exceed their guideline values and did not cause non-carcinogenic and carcinogenic health impacts through seafood product consumption. The pollution index (Pi) values of TMs in seafood products ranged from 0.01 to 0.7, with As and Cd showing the lowest and highest values, which were below 0.7, indicating their safety for consumption. The metal pollution index (MPI) for TMs in seafood ranged from 9.E-05 to 0.007, with the lowest and highest values associated with PP and TIO products. The MPI value for seafood products was below 1, signifying low TMs accumulation, suggesting the safety of seafood for consumption. The maximum seafood meal consumption limit (CRmm) was calculated, showing that adults can safely consume > 16 meals/month without chronic or acute toxicity. The results of this study suggest that the accumulation of TMs in the analyzed seafood product was below the health guidelines and pollution index values, making it suitable for smooth domestic and international export as well as safe consumption.

Species distribution models to predict the impacts of environmental disasters on shrimp species of economic interest.

Here, we used distribution models to predict the size of the environmentally suitable area for shrimps of fishing interest that were impacted by the tailing plume from the collapse of the Fundão Dam, one of the largest ecological disasters ever to occur in Brazil. Species distribution models (SDMs) were generated for nine species of penaeid shrimp that occurred in the impacted region. Average temperature showed the highest percentage of contribution for SDMs. The environmental suitability of penaeids varied significantly in relation to the distance to the coast and mouth river. The area of environmental suitability of shrimps impacted by tailings plumes ranged from 27 to 47 %. Notably, three protected areas displayed suitable conditions, before the disaster, for until eight species. The results obtained by the SDMs approach provide crucial information for conservation and restoration efforts of coastal biodiversity in an impacted region with limited prior knowledge about biodiversity distribution.

Assessment of microplastic contamination in shrimps from the Bay of Bengal and associated human health risk.

Microplastics (MPs) were analyzed in seven shrimp species Tiger shrimp (Penaeus monodon), Red tiger shrimp (Caridina cantonensis), Indian shrimp (Penaeus indicus), Red shrimp (Metapenaeus dobsoni), White shrimp (Penaeus merguiensis), Brown shrimp (Metapenaeus monoceros), and Roshna shrimp (Palaemon styliferus) collected from the Bay of Bengal. The abundance and characteristics of MPs were assessed in the gastrointestinal tract (GIT), which certainly translocated to the muscle of shrimp species. The highest MP abundance was found in C. cantonensis with 7.2 items/individual (25.3 items/g in the GIT and 6.3 items/g in muscle). The prominent types of MPs in shrimp samples were fibers (30 %) and fragments (29 %). The ingestion rate of MPs of black and transparent color was comparatively higher, with 64 % of the ingested MPs were < 100 µm. The primary polymer types detected based on Fourier Transform Infrared (FTIR) analysis were Low-Density Polyethylene (LDPE), High-Density Polyethylene (HDPE), Polymethyl Methacrylate (PMMA), Polyvinyl Chloride (PVC), Polypropylene (PP), and Ethylene Vinyl Acetate (EVA). Results from Scanning Electron Microscopy (SEM) showed rough surface textures and adhered particles on the MPs isolated from shrimps. The margin of exposure for females was 71.42, and for males, it was 80.64, indicating that women in Bangladesh are more likely to be exposed to MPs and face a higher risk than men. Sensitivity analysis revealed that MPs particle size was the most sensitive parameter. These findings provide a comprehensive understanding of MP ingestion, human exposure, and contamination in shrimps of Bangladesh, which can help future monitoring efforts.

Application of a stoichiometric bioenergetic approach and whole-body protein synthesis to the nutritional assessment of juvenile Thenus australiensis.

The present study successfully combined a stoichiometric bioenergetic approach with an endpoint stochastic model to simultaneously determine specific dynamic action, metabolic substrate use and whole-body protein synthesis in juvenile slipper lobster Thenus australiensis. Juvenile lobsters were fasted for 48 h to investigate routine metabolism before receiving a single meal of formulated feed containing 1% 15N-labeled Spirulina. Postprandial oxygen consumption rate, dissolved inorganic carbon, and total nitrogen excretion returned to the pre-feeding level within 24 h. The rate of whole-body protein synthesis was 0.76 ± 0.15 mg CP g-1 day-1, with a significant reduction from 24 to 48 h post-feeding. The postprandial increase in whole-body protein synthesis accounted for 13-19% of total oxygen uptake. Protein was the primary energy substrate for 48 h fasted (45% oxygen consumption) and post-feeding lobster (44%), suggesting that dietary protein was not efficiently used for growth. The secondary energy substrate differed between carbohydrates in 48 h fasted and lipids in post-feeding lobsters. The present study recommends integrating protein synthesis into protein requirement experiments of marine ectotherms to acquire a more comprehensive picture of protein and energy metabolism and nutritional physiology crucial for formulating cost-effective aquafeeds.

Assessment of genetic diversity and population structure of Neocaridina denticulata sinensis in the Baiyangdian drainage area, China, using microsatellite markers and mitochondrial cox1 gene sequences.

Neocaridina denticulata sinensis is a crustacean of major economic significance in the Baiyangdian drainage area. In this study, the first assessment of N. denticulata sinensis genetic diversity and population structure was performed based on sequence analysis of nine polymorphic microsatellite loci and the mitochondrial cytochrome oxidase subunit I (cox1) gene. Samples (n = 192) were collected from four different regions in the Baiyangdian drainage area i.e., Baiyangdian Lake, Jumahe River, Xidayang Reservoir, and Fuhe River. Microsatellite loci analysis identified high levels of genetic diversity represented by observed heterozygosity (Ho) of 0.6865 âˆ¼ 0.9583, expected heterozygosity (He) of 0.7151 âˆ¼ 0.8723, and polymorphism information content (PIC) of 0.6676 âˆ¼ 0.8585. Based on the analysis of cox1 sequences, haplotype diversity (Hd) ranged from 0.568 to 0.853 while nucleotide diversity (π) ranged from 0.0029 to 0.2236. Furthermore, there was no evidence of expansion events in the N. denticulata sinensis populations. Pairwise FST revealed pronounced genetic differentiation, and clustering analyses showed defined genetic structures within the N. denticulata sinensis population. Three groups were identified from four sampled stocks, with Xidayang Reservoir, and Fuhe River populations clustered in the same group. This work identified novel molecular markers and provided an important reference to guide management strategies to assist conservation of N. denticulata sinensis resources.

Environmental and economic impacts of retrieved abandoned, lost, and discarded fishing gear in Southwest Nova Scotia, Canada.

Abandoned, lost, and discarded fishing gear (ALDFG), negatively impacts marine environments. Managing ALDFG in Atlantic Canada is challenging due to knowledge gaps on loss rates, locations, data availability/accuracy, impacts, and regulatory barriers for retrieval. This study removed ALDFG in Southwest Nova Scotia in collaboration with local fishers (with local knowledge and practical ALDFG removal expertise), government, non-profit organizations, and academia. A total of 29,298 kg of ALDFG was retrieved, including 24,630 kg using towed grapples covering ~3986 km of seafloor and 4668 kg from shorelines (comprising, 68 % lobster traps and 12 % dragger cable by weight). Traps ranged from <1 to 37 years old (median, 10 years). Traps continued to catch target and non-target species with 25 species released, including 652 individual lobsters (82 % were market-sized) and 57 fish (42 were species-at-risk). Based on estimated 2 % trap losses, annual commercial losses from ALDFG were $155,836 CAD in Lobster Fishing Area 34.

Demographic inference provides insights into the extirpation and ecological dominance of eusocial snapping shrimps.

Although eusocial animals often achieve ecological dominance in the ecosystems where they occur, many populations are unstable, resulting in local extinction. Both patterns may be linked to the characteristic demography of eusocial species-high reproductive skew and reproductive division of labor support stable effective population sizes that make eusocial groups more competitive in some species, but also lower effective population sizes that increase susceptibility to population collapse in others. Here, we examine the relationship between demography and social organization in Synalpheus snapping shrimps, a group in which eusociality has evolved recently and repeatedly. We show using coalescent demographic modeling that eusocial species have had lower but more stable effective population sizes across 100,000 generations. Our results are consistent with the idea that stable population sizes may enable competitive dominance in eusocial shrimps, but they also suggest that recent population declines are likely caused by eusocial shrimps' heightened sensitivity to environmental changes, perhaps as a result of their low effective population sizes and localized dispersal. Thus, although the unique life histories and demography of eusocial shrimps have likely contributed to their persistence and ecological dominance over evolutionary time scales, these social traits may also make them vulnerable to contemporary environmental change.

Identifying economic costs and knowledge gaps of invasive aquatic crustaceans.

Despite voluminous literature identifying the impacts of invasive species, summaries of monetary costs for some taxonomic groups remain limited. Invasive alien crustaceans often have profound impacts on recipient ecosystems, but there may be great unknowns related to their economic costs. Using the InvaCost database, we quantify and analyse reported costs associated with invasive crustaceans globally across taxonomic, spatial, and temporal descriptors. Specifically, we quantify the costs of prominent aquatic crustaceans - crayfish, crabs, amphipods, and lobsters. Between 2000 and 2020, crayfish caused US$ 120.5 million in reported costs; the vast majority (99%) being attributed to representatives of Astacidae and Cambaridae. Crayfish-related costs were unevenly distributed across countries, with a strong bias towards European economies (US$ 116.4 million; mainly due to the signal crayfish in Sweden), followed by costs reported from North America and Asia. The costs were also largely predicted or extrapolated, and thus not based on empirical observations. Despite these limitations, the costs of invasive crayfish have increased considerably over the past two decades, averaging US$ 5.7 million per year. Invasive crabs have caused costs of US$ 150.2 million since 1960 and the ratios were again uneven (57% in North America and 42% in Europe). Damage-related costs dominated for both crayfish (80%) and crabs (99%), with management costs lacking or even more under-reported. Reported costs for invasive amphipods (US$ 178.8 thousand) and lobsters (US$ 44.6 thousand) were considerably lower, suggesting a lack of effort in reporting costs for these groups or effects that are largely non-monetised. Despite the well-known damage caused by invasive crustaceans, we identify data limitations that prevent a full accounting of the economic costs of these invasive groups, while highlighting the increasing costs at several scales based on the available literature. Further cost reports are needed to better assess the true magnitude of monetary costs caused by invasive aquatic crustaceans.

Osmoregulatory power influences tissue ionic composition after salinity acclimation in aquatic decapods.

Decapod crustaceans show variable degrees of euryhalinity and osmoregulatory capacity, by responding to salinity changes through anisosmotic extracellular regulation and/or cell volume regulation. Cell volume regulatory mechanisms involve exchange of inorganic ions between extra- and intra-cellular (tissue) compartments. Here, this interplay of inorganic ions between both compartments has been evaluated in four decapod species with distinct habitats and osmoregulatory strategies. The marine/estuarine species Litopenaeus vannamei (Lv) and Callinectes danae (Cd) were submitted to reduced salinity (15‰), after acclimation to 25 and 30‰, respectively. The freshwater Macrobrachium acanthurus (Ma) and Aegla schmitti (As) were submitted to increased salinity (25‰). The four species were salinity-challenged for both 5 and 10 days. Hemolymph osmolality, sodium, chloride, potassium, and magnesium were assayed. The same inorganic ions were quantified in muscle samples. Muscle hydration (MH) and ninhydrin-positive substances (NPS) were also determined. Lv showed slight hemolymph dilution, increased MH and no osmotically-relevant decreases in muscle osmolytes; Cd displayed hemolymph dilution, decreased muscular NaCl and stable MH; Ma showed hypo-regulation and steady MH, with no change in muscle ions; As conformed hemolymph sodium but hypo-regulated chloride, had stable MH and increased muscle NPS and ion levels. Hemolymph and muscle ions (especially chloride) of As were highly correlated (Pearson, +0.83). Significant exchanges between hemolymph and muscle ionic pools were more evident in the two species with comparatively less AER regulatory power, C. danae and A. schmitti. Our findings endorse that the interplay between extracellular and tissue ionic pools is especially detectable in euryhaline species with relatively lower osmoregulatory strength.

A capability approach to assess aquaculture sustainability standard compliance.

Sustainability standards are used to assure improved environmental performance in the aquaculture sector. But standard setters face limitations in including a broad range of producers with different capabilities, which in turn reduces their scope and impact. Drawing on Sen's capability approach, we introduce a novel way to assess the extent to which sustainability standards can support the capability of farmers to make prescribed improvements to their production practices. In doing so, we compare four shrimp aquaculture standards (Aquaculture Stewardship Council, Global Aquaculture Alliance, Southeast Asian Shrimp Aquaculture Improvement Protocol and the Thai Agricultural Standard) based on an analysis of what we label the 'prescribed capitals' and 'bundle of capitals' that underpin the compliance capability of producers. The results show that standards narrowly prescribe standards requiring human capital, while there is potential for prescribing a wider bundle of social, financial and physical capitals that can allow more flexible standard compliance. The findings raise the prospect of redesigning sustainability standards to support a broader diversity of producer capabilities and, in turn, increase their overall impact.

A Mesocosm Assessment of the Effect of Bioturbation by the Ghost Shrimp (Lepidophthalmus louisianensis) on the Fate of Petroleum Hydrocarbons in the Intertidal Zone.

To fully assess the long-term impacts of oil spills like the 2010 Deepwater Horizon incident in the northern Gulf of Mexico, the potential for organisms other than microbes to affect the fate and distribution of the oil may have to be considered. This influence could be substantial for abundant bioturbating benthic animals like the ghost shrimp Lepidophthalmus louisianensis. An assessment of the influence of these ghost shrimp on petroleum hydrocarbons was conducted in laboratory micro- and mesocosms containing coastal Gulf of Mexico sediment, seawater, and oil or the polynuclear aromatic hydrocarbon (PAH) pyrene. In an experiment with pyrene added to the water column, the ghost shrimp presence lowered water-column pyrene concentrations. In an experiment with oil added to the sediment surface, the ghost shrimp presence decreased PAH concentrations in the sediment surface layer but increased these in the water column and subsurface sediment. A companion study and a mass-balance analysis indicated a net loss of PAHs through an enhancement of microbial degradation. In an experiment in which oil was added as a narrow subsurface layer in the sediment, the ghost shrimp presence appeared to broaden the oil's depth distribution. Taken together, these results demonstrate that ghost shrimp can significantly influence the biodegradation and distribution of spilled oil in coastal ecosystems. Environ Toxicol Chem 2020;39:637-647. © 2019 SETAC.

Assessment of loss of 210Po from fish and shrimp by cooking and its effect on dose estimates to humans ingesting seafood.

An experimental study was conducted to assess loss of 210Po due to cooking fresh seafood, and provide a more realistic and reliable dose estimate that humans may receive from consuming cooked seafood. Fresh fish and shrimp samples from Northern Gulf waters were grilled and boiled to simulate the effect of different cooking methods. Sixteen different species of fish were compared and significant differences in 210Po concentration in uncooked samples were observed between species (ANOVA I, F15,79 = 362.81, p < 0.0001). The effect of the treatment (uncooked, grilled, boiled and stock) was compared for each species and it was found that cooking led to a significant decrease in 210Po concentration ranging from 14 to 58% compared to the uncooked samples, with no difference between grilled or boiled treatments. The effect of the cooking and shrimp treatment on 210Po concentration was tested using ANOVA II after logarithmic transformation. Cooking led to a significant 38% reduction of 210Po concentration as compared to uncooked treatments with no difference between grilled and boiled samples (ANOVA I: F3,99 = 14.72, p < 0.0001). The two treatments with deveined shrimp led to a 75% decrease in 210Po concentration as compared to all other treatments. As a consequence, cooked deveined shrimp contained an 84% lower 210Po concentration than whole uncooked shrimp. As 210Po is known to be the major contributor to radiation dose in humans consuming seafood, based on the results obtained, it is evident there is a need to re-examine how committed effective doses (CEDs) are best calculated for seafood consuming populations considering that most populations consume fish and shellfish cooked.

A phylogenomic framework, evolutionary timeline and genomic resources for comparative studies of decapod crustaceans.

Comprising over 15 000 living species, decapods (crabs, shrimp and lobsters) are the most instantly recognizable crustaceans, representing a considerable global food source. Although decapod systematics have received much study, limitations of morphological and Sanger sequence data have yet to produce a consensus for higher-level relationships. Here, we introduce a new anchored hybrid enrichment kit for decapod phylogenetics designed from genomic and transcriptomic sequences that we used to capture new high-throughput sequence data from 94 species, including 58 of 179 extant decapod families, and 11 of 12 major lineages. The enrichment kit yields 410 loci (greater than 86 000 bp) conserved across all lineages of Decapoda, more clade-specific molecular data than any prior study. Phylogenomic analyses recover a robust decapod tree of life strongly supporting the monophyly of all infraorders, and monophyly of each of the reptant, 'lobster' and 'crab' groups, with some results supporting pleocyemate monophyly. We show that crown decapods diverged in the Late Ordovician and most crown lineages diverged in the Triassic-Jurassic, highlighting a cryptic Palaeozoic history, and post-extinction diversification. New insights into decapod relationships provide a phylogenomic window into morphology and behaviour, and a basis to rapidly and cheaply expand sampling in this economically and ecologically significant invertebrate clade.

Molecular identification and phylogenetic assessment of species under genus Parapenaeopsis Alcock, 1901, from Indian waters.

Genus Parapenaeopsis constitute an important fishery resource in Indian waters. Parapenaeopsis stylifera, being morphologically very similar to Parapenaeopsis coromandelica, mainly differentiated using telsonic armature and it is subject of controversy regarding the number of armature for the species. The present study was aimed to resolve the taxonomic ambiguity between P. stylifera and P. coromandelica and to study phylogenetic relationship among five species under the genus Parapenaeopsis using partial sequences of mitochondrial 16S ribosomal DNA (rDNA) and cytochrome c oxidase subunit I (COI) genes. Sequence analysis revealed an average genetic divergence value ranging from 7.65 to 20.3% for COI gene and 2.4 to 12.0% for 16S rDNA gene between five species under study. The result shows that P. stylifera and P. coromandelica Alcock, 1906 are genetically a distinct species with average divergence value of 7.6% based on COI sequence and 2.4% based on the 16S rDNA. However, systematic relationship between the P. stylifera and P. coromandelica were closer than other species, thus formed separate clade in phylogenetic tree. The species specific molecular signatures developed in the present study will be helpful in effective fisheries management of the species in Indian waters.

Checklists of Crustacea Decapoda from the Canary and Cape Verde Islands, with an assessment of Macaronesian and Cape Verde biogeographic marine ecoregions.

The complete list of Canarian marine decapods (last update by González Quiles 2003, popular book) currently comprises 374 species/subspecies, grouped in 198 genera and 82 families; whereas the Cape Verdean marine decapods (now fully listed for the first time) are represented by 343 species/subspecies with 201 genera and 80 families. Due to changing environmental conditions, in the last decades many subtropical/tropical taxa have reached the coasts of the Canary Islands. Comparing the carcinofaunal composition and their biogeographic components between the Canary and Cape Verde archipelagos would aid in: validating the appropriateness in separating both archipelagos into different ecoregions (Spalding et al. 2007), and understanding faunal movements between areas of benthic habitat. The consistency of both ecoregions is here compared and validated by assembling their decapod crustacean checklists, analysing their taxa composition, gathering their bathymetric data, and comparing their biogeographic patterns. Four main evidences (i.e. different taxa; divergent taxa composition; different composition of biogeographic patterns; different endemicity rates) support that separation, especially in coastal benthic decapods; and these parametres combined would be used as a valuable tool at comparing biotas from oceanic archipelagos. To understand/predict south-north faunal movements in a scenario of regional tropicalization, special attention is paid to species having at the Canaries their southernmost occurrence, and also to tropical African warm-affinity species.

Allometry of individual reproduction and defense in eusocial colonies: A comparative approach to trade-offs in social sponge-dwelling Synalpheus shrimps.

Eusociality, one of the most complex forms of social organization, is thought to have evolved in several animal clades in response to competition for resources and reproductive opportunities. Several species of snapping shrimp in the genus Synalpheus, the only marine organisms known to exhibit eusociality, form colonies characterized by high reproductive skew, and aggressive territoriality coupled with cooperative defense. In eusocial Synalpheus colonies, individual reproduction is limited to female 'queens', whose fecundity dictates colony growth. Given that individual reproduction and defense are both energetically costly, individual and colony fitness likely depend on the optimal allocation of resources by these reproducing individuals towards these potentially competing demands. Synalpheus species, however, display varying degrees of eusociality, suggesting that reproducing females have adopted different strategies for allocation among reproduction and defense. Here, we use structural equation modeling to characterize the relationships between the allometry of queen reproductive capacity and defensive weaponry, and colony size in six eusocial Synalpheus species, estimating trade-offs between reproduction and defense. We document strong trade-offs between mass of the fighting claw (defense) and egg number (reproduction) in queens from weakly eusocial species, while the trade-off is reduced or absent in those from strongly eusocial species. These results suggest that in less cooperative species, intra-colony conflict selects for queen retention of weapons that have significant costs to fecundity, while reproducing females from highly eusocial species, i.e., those with a single queen, have been able to reduce the cost of weapons as a result of protection by other colony members.

Assessment of sperm quality in palaemonid prawns using Comet assay: methodological optimization.

The aim of this study was to adapt the Comet assay in spermatozoa of the marine prawn Palaemon serratus to use it as a marker of sperm quality. Indeed, due to the characteristics of their spermatozoa, the measurement of DNA integrity is one of the few markers which can be transferred to crustaceans to assess the quality of their semen. In the first step, the methods of collecting and maintaining spermatozoa were optimized. Cell survival was estimated during kinetics of preservation (i.e. 1, 2, 4 and 8 h) in various suspension media to define artificial seawater (ASW) as optimal. Several methods in the releasing of spermatozoa from the spermatophore of prawns were estimated with regard to their incidence both on the efficiency of extraction and the survival of cells. Pipetting up and down turned out to be the most successful and the least invasive technique. Secondly, the transfer of Comet assay was optimized by studying various times in both cell lysis (i.e. 1, 6, 18 h) and DNA denaturation (i.e. 15, 30 and 45 min), after in vitro exposure of spermatozoa to an H2O2 gradient as model genotoxicant. Results revealed that a minimum of 1 h in cell lysis and 15 min of DNA denaturation were sufficient to obtain valuable results, linked with a low compaction of DNA in spermatozoa of Palaemon sp. Finally, the sensitivity of P. serratus spermatozoa was assessed after in vitro exposures to model genotoxicants displaying various modes of interaction with DNA (i.e. UV-C, 13.3-79.5 J m-2; H2O2, 5-10 µM and MMS, 0.5-5 mM) and some environmental contaminants known or suspected to be genotoxic (i.e. cadmium and diuron, 0.015-1.5 µg L-1; carbamazepine, 0.1-10 µg L-1) for invertebrates. The low variability of the baseline level of DNA strand breaks recorded in controls highlighted the robustness of the method. P. serratus spermatozoa displayed significant DNA damage from the lowest doses tested for all model genotoxicants, but conversely, no genotoxic effect of tested environmental contaminants was observed. These results, which are discussed according to the protocol tested in the present study and the comparison with literature data, could suggest a difference in the response or sensitivity of spermatozoa to environmental genotoxicity between invertebrate species, and therefore the interest of Palaemonidae prawns in ecogenotoxicology. In conclusion, the present study underlines the potential of the Comet assay as a marker to assess the contamination impact on the sperm quality in Palaemonidae prawns in view to a potential application for in situ biomonitoring surveys.

Short-term impacts and value of a periodic no take zone (NTZ) in a community-managed small-scale lobster fishery, Madagascar.

The small-scale lobster fisheries of Madagascar's impoverished southeast coast account for the majority of national catch and export, making a significant contribution to the regional economy. Data suggests catches have declined, likely due to over-exploitation. In response, the community of Sainte Luce has established a locally managed marine area (LMMA) to manage their lobster fishery, including a 13 km2 periodic no take zone (NTZ). Participatory monitoring data were used to assess compliance, identify changes in catch per unit effort (CPUE) associated with the NTZ and consider the short-term value of the NTZ. Compliance is low for measures dictated by national legislation (minimum landing size (MLS), national closed season, prohibition on landing berried females), but may be higher for those designed by the community (NTZ). Upon NTZ opening in July 2015, an estimated 435% increase in catch was observed compared with the mean for the preceding five months, a product of increased effort and significantly higher CPUE. Zero Altered Negative Binomial modelling showed CPUE was significantly higher during the NTZ opening in 2015 and in 2016 when the opening period had been moved. Whilst it is unlikely that tangible ecological benefits have accrued from NTZ operation, there have been important socio-economic effects. Specifically, there was a 33% increase in the price fishers received, a significant effect at the bottom of the value chain. Temporary increases in catch and income acted as a catalyst, engaging neighbouring communities in fishery management, resulting in two additional NTZs. Attention is drawn to the fact that current national legislation may be sub-optimal and should be reviewed. Successful management of the regional fishery will require the state and industry to support communities in adopting community-based management. The NTZ measure considered here may be an effective tool to achieve this.

Bacillus Probiotic Enzymes: External Auxiliary Apparatus to Avoid Digestive Deficiencies, Water Pollution, Diseases, and Economic Problems in Marine Cultivated Animals.

Exploitation of marine fishes is the main source of several life-supporting feed compounds such as proteins, lipids, and carbohydrates that maintain the production of most trading marine organisms by aquaculture. However, at this rate the marine inventory will go to the end soon, since fishery resources are finite. In this sense, the availability of the principal ingredients obtained from marine fishes is going to decrease considerably, increasing the diet prices and affecting the economy of this activity. Therefore, aquaculture industry needs to find nonexpensive land unconventional resources of protein, carbohydrates, and lipids and use bacterial probiotics to improve digestion-assimilation of these unfamiliar compounds. Bacillus subtilis is a cosmopolitan probiotic bacterium with a great enzymatic profile that could improve nutrient digestion-assimilation, induce healthy growth, and avoid water pollution, decreasing economic problems and increasing yields in the aquaculture industry. In this chapter, we present how Bacillus enzymes can help marine animals to assimilate nutrients from unconventional and economic plant resources.