RESUMO
PURPOSE: To assess bone regeneration potential of a fibronectin- and adipose-derived stem cell-covered ceramic biomaterial in three-wall critical-size alveolar ridge defects. MATERIALS AND METHODS: In 18 dogs, four dehiscence-type and critical-size defects were created surgically in the edentulous alveolar ridge. Defects were randomly regenerated using biomaterials coated with particulate ß-tricalcium phosphate (ß-TCP), ß-TCP with fibronectin (Fn) (ß-TCP-Fn), and ß-TCP with a combination of Fn and autologous adipose-derived stem cells (ADSCs) (ß-TCP-Fn-ADSCs), leaving one defect as control. The animals were divided into three groups according to the time of euthanasia (1, 2, or 3 months of healing). RESULTS: At the time of sacrifice, statistically significant differences between the four types of defects in the total area of bone regeneration, percentage of neoformed bone matrix, medullary space, or contact between particulate biomaterial and neoformed bone matrix were not found. All defects showed a significant increase in neoformed bone matrix as sacrifice was delayed, but a uniform pattern was not followed. Only defects treated with ß-TCP-Fn-ADSCs showed a significant increase in the bone regeneration area when animals sacrificed at 3 months were compared to those sacrificed at 1 month (P = .006). CONCLUSION: The use of ADSCs in bone regeneration processes of critical-size defects of the alveolar ridge did not entail an advantage regarding greater bone regeneration as compared with other biomaterials. However, the use of ß-TCP coated with a combination of Fn and ADSCs appeared to favor stabilization of the regenerated area, allowing a more efficient maintenance of the space at 3 months of healing.
Assuntos
Tecido Adiposo/citologia , Perda do Osso Alveolar/terapia , Regeneração Óssea/fisiologia , Transplante de Células-Tronco/métodos , Gordura Abdominal/citologia , Animais , Materiais Biocompatíveis/química , Medula Óssea/patologia , Medula Óssea/fisiologia , Matriz Óssea/patologia , Matriz Óssea/fisiologia , Fosfatos de Cálcio/química , Cerâmica/química , Cães , Feminino , Fibronectinas/uso terapêutico , Regeneração Tecidual Guiada/métodos , Mandíbula/patologia , Doenças Mandibulares/terapia , Osteogênese/fisiologia , Distribuição Aleatória , Células-Tronco/fisiologia , Fatores de Tempo , Alicerces Teciduais/químicaAssuntos
Transfusão de Sangue Autóloga/métodos , Transfusão de Sangue/métodos , Procedimentos Cirúrgicos Cardíacos , Adulto , Ácido Aminocaproico/uso terapêutico , Anticoagulantes/uso terapêutico , Antifibrinolíticos/uso terapêutico , Transfusão de Sangue/economia , Transfusão de Sangue/instrumentação , Transfusão de Sangue Autóloga/economia , Transfusão de Sangue Autóloga/instrumentação , Controle de Custos , Tomada de Decisões , Transfusão de Eritrócitos , Eritropoetina/uso terapêutico , Fator VIII/uso terapêutico , Feminino , Fibrinogênio/uso terapêutico , Fibronectinas/uso terapêutico , Hematócrito , Hemodiluição , Heparina/uso terapêutico , Hospitais Universitários , Humanos , Masculino , Plasma , Transfusão de Plaquetas , Guias de Prática Clínica como Assunto , Resultado do Tratamento , VirginiaRESUMO
The A.A. studied the effectiveness of colliriums containing fibronectins aprotinin, and fibronectin associated with aprotinin compared to a placebo using 12 rabbits in which a deep corneal ulcer was induced by alkal. The results showed the same effectiveness of the three collyrium versus the placebo there was clinical recovery in all cases and restoration of corneal trasparency for 13 out of 15 treated eyes. Cellular cultures obtained from primary explants of treated corneal cells, compared with non treated groups, gave the same outcome of the clinical's ones.