RESUMO
Antifilarial potential of three medicinal plants namely, Terminalia bellerica, Terminalia chebula and Terminalia catappa was explored using Setaria cervi, a bovine filarial parasite at concentrations of 2.5, 5 and 10mg/ml. Amongst all the extracts, methanol extract of T. bellerica showed highest macrofilaricidal activity i.e. 84.63±1.11 at 10mg/ml in MTT reduction assay with IC50 value of 2.7mg/ml. which was better than the standard DEC i.e. 79.22±3.1% at 10mg/ml with IC50 value 2.84mg/ml. Other plant extracts showed mild in vitro macrofilaricidal activity. T. bellerica methanol extract exhibited significant GST activity of 18.86±0.21 and 12.83±0.03µM/ml/min at 5 and 10mg/ml with percentage inhibition value of 73.96% and 82.29% respectively. DEC showed GST activity value of 40.03±4.14 and 21.48±6.44µM/ml/min with percentage inhibition value of 21.76% and 58.01% at 5 and 10mg/ml respectively. Thus, methanol extract of leaves of T. bellerica exhibited highly significant antifilarial potential and needs detailed analysis.
Assuntos
Filaricidas/farmacologia , Filarioidea/efeitos dos fármacos , Extratos Vegetais/farmacologia , Terminalia/química , Animais , Bovinos , Doenças dos Bovinos/parasitologia , Sobrevivência Celular/efeitos dos fármacos , Filariose/parasitologia , Filariose/veterinária , Filaricidas/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Folhas de Planta/químicaRESUMO
A total of 32,042 cattle, slaughtered at the Pretoria, Johannesburg and Durban abattoirs, was examined for parafilariosis and as many animals as possible were traced to the farms from which they originated. The disease is most prevalent in the Bushveld areas of the northern Transvaal. The most important factors limiting its distribution are the annual rainfall and the duration of the frost period. Mention is made of some aspects of the economic loss involved. The effect of routine anthelmintic treatment and dipping on the prevalence of the parasite is briefly discussed.