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1.
J Food Sci ; 77(4): C333-9, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22429318

RESUMO

Effect of acylation with saturated fatty acids on surface functional properties of tofu whey-derived peptides was investigated. Tofu whey (TW) and soy proteins (7S, 11S, and acid-precipitated soy protein [APP]) were hydrolyzed by Protease M 'Amano' G, and resulting peptide mixtures were acylated with esterified fatty acids of different chain length (6C to 18C) to form a covalent linkage between the carboxyl group of fatty acid and the free amino groups of peptide. Acylation significantly (P < 0.05) increased emulsifying properties of 7S, 11S, and APP peptides independent of fatty acid chain length. Acylation decreased water binding capacity although oil binding capacity of acylated tofu whey ultra filtered fraction (UFTW < 3 kDa), 7S- and 11S-peptides were improved compared to native peptides. 7S peptides acylated with long chain fatty acids had shown significant higher surface hydrophobicity as in contrast with acylated UFTW < 3 kDa and APP peptides. Fluorescence spectra studies revealed structural conformation of acylated soy peptides as compared to native peptides. This study shows that chemical modification with fatty acids can further affect functional properties of soy proteins.


Assuntos
Emulsificantes/química , Ácidos Graxos/química , Aditivos Alimentares/química , Lipopeptídeos/química , Fragmentos de Peptídeos/química , Proteínas de Vegetais Comestíveis/química , Proteínas de Soja/química , Acilação , Antígenos de Plantas/química , Antígenos de Plantas/metabolismo , Fenômenos Químicos , Emulsificantes/economia , Emulsificantes/isolamento & purificação , Emulsificantes/metabolismo , Endopeptidases/metabolismo , Aditivos Alimentares/economia , Aditivos Alimentares/isolamento & purificação , Aditivos Alimentares/metabolismo , Indústria de Processamento de Alimentos/economia , Globulinas/química , Globulinas/metabolismo , Interações Hidrofóbicas e Hidrofílicas , Resíduos Industriais/análise , Resíduos Industriais/economia , Lipopeptídeos/isolamento & purificação , Lipopeptídeos/metabolismo , Peso Molecular , Fragmentos de Peptídeos/isolamento & purificação , Fragmentos de Peptídeos/metabolismo , Proteínas de Vegetais Comestíveis/isolamento & purificação , Proteínas de Vegetais Comestíveis/metabolismo , Conformação Proteica , Proteínas de Armazenamento de Sementes/química , Proteínas de Armazenamento de Sementes/metabolismo , Proteínas de Soja/isolamento & purificação , Proteínas de Soja/metabolismo , Propriedades de Superfície , Ultrafiltração , Água/análise
2.
Am J Vet Res ; 66(3): 375-8, 2005 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-15822578

RESUMO

OBJECTIVE: To determine the reliability of plasma electrophoresis (EPH) in psittacine birds. ANIMALS: 93 psittacine birds. PROCEDURE: Jugular venipuncture was performed on 93 awake psittacine birds. The plasma was centrifuged, separated, aliquoted into duplicate samples, frozen, and sent to 2 commercial laboratories that routinely perform avian EPH. Samples from 51 birds were sent to laboratory A, and samples from 42 birds were sent to laboratory B. The reliability of EPH results within each laboratory was assessed, but not between laboratories. To determine the reliability (agreement between duplicate samples) of total protein, albumin, prealbumin, alpha1-, alpha2-, beta-, and gamma-globulin concentrations, the intraclass correlation coefficient (r(i)) was calculated. RESULTS: Both laboratories had excellent agreement between samples for measurement of total protein concentration and only good agreement for albumin concentration. Except for the prealbumin concentration measured at laboratory B, both laboratories had poor agreement for all other values of the EPH. CONCLUSIONS AND CLINICAL RELEVANCE: These data indicate that plasma EPH for measuring prealbumin, alpha1-, alpha2-, beta-, and gamma-globulin concentrations may not be a reliable tool for assessing avian health. Small amounts of these proteins in birds plus human variation in reading the EPH curves may lead to variable results. Avian veterinarians should cautiously interpret results from plasma EPH assays for these protein fractions.


Assuntos
Proteínas Sanguíneas/análise , Psittaciformes/sangue , Animais , Eletroforese das Proteínas Sanguíneas/métodos , Eletroforese das Proteínas Sanguíneas/veterinária , Estudos de Avaliação como Assunto , Globulinas/metabolismo , Reprodutibilidade dos Testes
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