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1.
Appl Microbiol Biotechnol ; 102(24): 10511-10521, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30324487

RESUMO

In this study, the effect of several organic nitrogen sources (namely peptone, meat extract-ME, yeast extract-YE, and corn steep liquor-CSL) on D-lactic acid production by Lactobacillus delbrueckii ssp. delbrueckii has been studied. While lactic acid bacteria (LAB) are well-known for their complex nutritional requirements, organic nitrogen source-related cost can be as high as 38% of total operational costs (OPEX), being its nature and concentration critical factors in the growth and productivity of the selected strain. Corn steep liquor (CSL) has been chosen for its adequacy, on the grounds of the D-lactic acid yield, productivity, and its cost per kilogram of product. Finally, orange peel waste hydrolysate supplemented with 37 g/l CSL has been employed for D-lactic acid production, reaching a final yield of 88% and a productivity of 2.35 g/l h. CSL cost has been estimated at 90.78$/ton of D-lactate.


Assuntos
Biotecnologia/métodos , Citrus sinensis , Ácido Láctico/biossíntese , Lactobacillus delbrueckii/metabolismo , Nitrogênio/metabolismo , Biotecnologia/economia , Custos e Análise de Custo , Resíduos Industriais , Peptonas/metabolismo , Leveduras/química , Leveduras/metabolismo
2.
J Sci Food Agric ; 96(11): 3831-7, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26692071

RESUMO

BACKGROUND: White mold-ripened cheeses were investigated with the objective of proposing a colorimetric method to monitor the surface growth of Penicillium candidum and to evaluate the influence of the mesophilic (homofermentative (QMO) and heterofermentative (QMLD)) and thermophilic (QT) starter cultures on the physicochemical composition and sensory description. RESULTS: The whiteness index was effective in proving the appearance of superficial mycelium and the stability of white mold growth. The lactic cultures showed significant influence on most of the physicochemical analyses. The cheese made with thermophilic lactic culture had a 1 day gain in the growth of mycelium on the surface; nevertheless, the appearance of this product was potentially not acceptable for consumers. The heterofermentative mesophilic cheese had a better appearance and texture profile. However, the homofermentative mesophilic cheese showed aspects of fresh cheese and was acceptable for a wide range of consumers. CONCLUSION: The whiteness index was efficient to monitor the surface growth of P. candidum. The highest proteolytic effect was found in the QMLD and QT cultures. However, the cheese elaborated with the QMLD culture showed the best sensory acceptance. © 2015 Society of Chemical Industry.


Assuntos
Queijo/análise , Inspeção de Alimentos , Qualidade dos Alimentos , Lactobacillus delbrueckii/crescimento & desenvolvimento , Lactococcus lactis/crescimento & desenvolvimento , Penicillium/crescimento & desenvolvimento , Streptococcus thermophilus/crescimento & desenvolvimento , Brasil , Queijo/microbiologia , Fenômenos Químicos , Fermentação , Preferências Alimentares , Humanos , Concentração de Íons de Hidrogênio , Lactobacillus delbrueckii/metabolismo , Lactococcus lactis/metabolismo , Fenômenos Mecânicos , Proteínas do Leite/análise , Proteínas do Leite/metabolismo , Penicillium/metabolismo , Análise de Componente Principal , Proteólise , Sensação , Streptococcus thermophilus/metabolismo , Propriedades de Superfície , Tirosina/análise , Tirosina/metabolismo
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