Collective movement of schooling fish reduces the costs of locomotion in turbulent conditions.

The ecological and evolutionary benefits of energy-saving in collective behaviors are rooted in the physical principles and physiological mechanisms underpinning animal locomotion. We propose a turbulence sheltering hypothesis that collective movements of fish schools in turbulent flow can reduce the total energetic cost of locomotion by shielding individuals from the perturbation of chaotic turbulent eddies. We test this hypothesis by quantifying energetics and kinematics in schools of giant danio (Devario aequipinnatus) and compared that to solitary individuals swimming under laminar and turbulent conditions over a wide speed range. We discovered that, when swimming at high speeds and high turbulence levels, fish schools reduced their total energy expenditure (TEE, both aerobic and anaerobic energy) by 63% to 79% compared to solitary fish (e.g., 228 versus 48 kj kg-1). Solitary individuals spend approximately 22% more kinematic effort (tail beat amplitude•frequency: 1.7 versus 1.4 BL s-1) to swim in turbulence at higher speeds than in laminar conditions. Fish schools swimming in turbulence reduced their three-dimensional group volume by 41% to 68% (at higher speeds, approximately 103 versus 33 cm3) and did not alter their kinematic effort compared to laminar conditions. This substantial energy saving highlights that schooling behaviors can mitigate turbulent disturbances by sheltering fish (within schools) from the eddies of sufficient kinetic energy that can disrupt locomotor gaits. Therefore, providing a more desirable internal hydrodynamic environment could be one of the ecological drivers underlying collective behaviors in a dense fluid environment.

Spatial confinement affects the heterogeneity and interactions between shoaling fish.

Living objects are able to consume chemical energy and process information independently from others. However, living objects can coordinate to form ordered groups such as schools of fish. This work considers these complex groups as living materials and presents imaging-based experiments of laboratory schools of fish to understand how activity, which is a non-equilibrium feature, affects the structure and dynamics of a group. We use spatial confinement to control the motion and structure of fish within quasi-2D shoals of fish and use image analysis techniques to make quantitative observations of the structures, their spatial heterogeneity, and their temporal fluctuations. Furthermore, we utilize Monte Carlo simulations to replicate the experimentally observed data which provides insight into the effective interactions between fish and confirms the presence of a confinement-based behavioral preference transition. In addition, unlike in short-range interacting systems, here structural heterogeneity and dynamic activities are positively correlated as a result of complex interplay between spatial arrangement and behavioral dynamics in fish collectives.

Neural Endophenotype Assessment in Zebrafish Larvae Using Optomotor and ZebraBox Locomotion Assessment.

Due to the highly conserved genetics across the central nervous system, the easily probed visual system can act as an endophenotype for assessing neurological function. Here, we describe a psychophysics approach to assess visually driven swimming behavior in the high-throughput zebrafish genetic model system. We use the optomotor response test together with general locomotion behavior to assess neural processing while excluding motor defects related to muscle function.

Dynamic body acceleration as a proxy to predict the cost of locomotion in bottlenose dolphins.

Estimates of the energetic costs of locomotion (COL) at different activity levels are necessary to answer fundamental eco-physiological questions and to understand the impacts of anthropogenic disturbance to marine mammals. We combined estimates of energetic costs derived from breath-by-breath respirometry with measurements of overall dynamic body acceleration (ODBA) from biologging tags to validate ODBA as a proxy for COL in trained common bottlenose dolphins (Tursiops truncatus). We measured resting metabolic rate (RMR); mean individual RMR was 0.71-1.42 times that of a similarly sized terrestrial mammal and agreed with past measurements that used breath-by-breath and flow-through respirometry. We also measured energy expenditure during submerged swim trials, at primarily moderate exercise levels. We subtracted RMR to obtain COL, and normalized COL by body size to incorporate individual swimming efficiencies. We found both mass-specific energy expenditure and mass-specific COL were linearly related with ODBA. Measurements of activity level and cost of transport (the energy required to move a given distance) improve understanding of the COL in marine mammals. The strength of the correlation between ODBA and COL varied among individuals, but the overall relationship can be used at a broad scale to estimate the energetic costs of disturbance and daily locomotion costs to build energy budgets, and investigate the costs of diving in free-ranging animals where bio-logging data are available. We propose that a similar approach could be applied to other cetacean species.

Fishes regulate tail-beat kinematics to minimize speed-specific cost of transport.

Energetic expenditure is an important factor in animal locomotion. Here we test the hypothesis that fishes control tail-beat kinematics to optimize energetic expenditure during undulatory swimming. We focus on two energetic indices used in swimming hydrodynamics, cost of transport and Froude efficiency. To rule out one index in favour of another, we use computational-fluid dynamics models to compare experimentally observed fish kinematics with predicted performance landscapes and identify energy-optimized kinematics for a carangiform swimmer, an anguilliform swimmer and larval fishes. By locating the areas in the predicted performance landscapes that are occupied by actual fishes, we found that fishes use combinations of tail-beat frequency and amplitude that minimize cost of transport. This energy-optimizing strategy also explains why fishes increase frequency rather than amplitude to swim faster, and why fishes swim within a narrow range of Strouhal numbers. By quantifying how undulatory-wave kinematics affect thrust, drag, and power, we explain why amplitude and frequency are not equivalent in speed control, and why Froude efficiency is not a reliable energetic indicator. These insights may inspire future research in aquatic organisms and bioinspired robotics using undulatory propulsion.

Establishing the reliability of instrumented trunk impairment assessment methods to enable evidence-based classification in Para swimming.

This study examined the reliability of instrumented trunk assessment methods across two experiments to develop and improve evidence-based classification in Para swimming. Trunk coordination, range of motion (ROM), and strength were assessed in 38 non-disabled participants. Each test battery was completed on two occasions to determine inter-session reliability. Intra-session reliability was also determined in Experiment Two. Absolute agreement of two-way mixed intraclass correlation coefficients (ICC 3,1) was calculated to assess reliability. Standard errors of measurement (SEMs) were also reported to facilitate comparisons between different outcomes. Trunk coordination measures had low-to-moderate reliability (inter-session ICCs = 0.00-0.60; intra-session ICCs = 0.14-0.65) and variable SEMs (5-60%). Trunk ROM demonstrated moderate-to-excellent reliability (inter-session ICCs = 0.61-0.93; intra-session ICCs = 0.87-0.95) and good SEMs (<10%). Trunk strength measures demonstrated good-to-excellent reliability (ICCs = 0.87-0.98) and good SEMs (<10%). The strength values obtained for the load cell and hand-held dynamometer (HHD) were significantly different from each other with the HHD underestimating strength. Modifications provided in Experiment Two improved the reliability of strength and ROM assessments but did not improve coordination measures. Further research involving para swimmers is required to establish the validity of the methods.

An Automated Low-Cost Swim Tunnel for Measuring Swimming Performance in Fish.

The study of swimming behavior is an important part of fish biology research and the swim tunnel is used to study swimming performance as well as metabolism of fish. In this investigation, we have developed a user-friendly, automated, modular, and low-cost swim tunnel that permits to study the performance of one or more fish separately, as well as a small group of individuals. To validate our swim tunnel, we assessed swimming activity of four different species (zebrafish, medaka, guppy, and cavefish) recording reliable data of swimming behavior and performance. Because swimming behavior has been recently used in different fields from physiology to ecotoxicology, our setup could help researchers with a low-cost solution.

Breathing through a diving snorkel; theory and experiment of air flow resistance and cost of breathing.

The snorkel allows a surface swimmer to observe the underwater world through the face mask without being disturbed by inhaling. The effect of a snorkel on breathing resistance and cost is widely held to be substantial. This study aims to model these parameters and to measure indirectly the actual increases. Further, resistances of differing designs and dimensions were assessed and recommendations were made concerning use and choice. Maximal voluntary ventilation in 12 seconds (MVV12) was measured in 19 volunteers seated on dry land with and without a classic J-type snorkel (inner diameter 20.5 mm). The extra and total resistances and costs were calculated using the MVV12 data and using estimated airways resistance extrapolated from subject's demography and spirometric literature data. MVV12 measurements with snorkel showed a minute volume of 152 ±38 L∙min-1, 6.0 ±3.7% lower than without snorkel (p = 7.0x10-6). The theoretical MVV12, calculated from snorkel and airways resistances, decreased by 3.2%. Experimental total breathing resistance (457±83 Pa∙s∙L-1) was 6.5 ± 3.2% higher than without snorkel (p = 2.6x10-7), but the total mechanical breathing cost was unaffected by the snorkel (13.58 Watts with; 13.64 Watts without). Divers' estimations of resistance increase were exaggerated (8.8% at rest, 23% swimming). Classical J-type snorkels with an inner diameter ≥19.5 mm add 3-16% resistance . There is no risk of hypercapnia. Scuba divers are recommended to use their snorkel to breathe more comfortably on the surface. It is recommended the snorkel be made a mandatory safety accessory. The best multipurpose snorkel (19-21 mm) has no top appendages and no water release valve.

Assessment of Structure, Function, and Rhythm of the Heart with Echocardiography and Electrocardiography in Adolescent Swimmers.

The aim of this study was to evaluate the cardiac parameters by using electrocardiography and echocardiography in adolescent swimmers. Twenty-two adolescent swimmers and 22 gender- and age-matched sedentary controls admitted to our center between November 2018 and May 2019 were included in this study. In addition to demographical characteristics, participants were assessed via a 12-lead electrocardiography and two-dimensional echocardiography for cardiac function. On the echocardiography, end-systolic and end-diastolic interventricular septum, end-systolic and end-diastolic left ventricular posterior wall thicknesses, left atrial width, Tricuspid E, left ventricular mass and left ventricular mass index were higher in the swimmers when compared to the sedentary controls (P < 0.05). On the electrocardiography, Tp-e duration which reflects ventricular transmural repolarization, and Tp-e/QT and Tp-e/corrected QT ratios were higher in the swimmers than the sedentary controls (P < 0.05). In conclusion, swimming exercise in children leads to concentric thickening of left ventricle and induces an increase in Tp-e duration, and Tp-e/QT and Tp-e/corrected QT ratios, which are the novel markers for risk of ventricular arrhythmias.

Effect of low- and high-carbohydrate diets on swimming economy: a crossover study.

BACKGROUND: Swimming economy refers to the rate of energy expenditure relative to swimming speed of movement, is inversely related to the energetic cost of swimming, and is as a key factor influencing endurance swimming performance. The objective of this study was to determine if high-carbohydrate, low-fat (HCLF) and low-carbohydrate, high-fat (LCHF) diets affect energetic cost of submaximal swimming. METHODS: Eight recreational swimmers consumed two 3-day isoenergetic diets in a crossover design. Diets were tailored to individual food preferences, and macronutrient consumption was 69-16-16% and 16-67-18% carbohydrate-fat-protein for the HCLF and LCHF diets, respectively. Following each 3-day dietary intervention, participants swam in a flume at velocities associated with 50, 60, and 70% of their maximal aerobic capacity (VO2max). Expired breath was collected and analyzed while they swam which enabled calculation of the energetic cost of swimming. A paired t-test compared macronutrient distribution between HCLF and LCHF diets, while repeated-measures ANOVA determined effects of diet and exercise intensity on physiological endpoints. RESULTS: Respiratory exchange ratio was significantly higher in HCLF compared to LCHF (p = 0.003), but there were no significant differences in the rate of oxygen consumption (p = 0.499) or energetic cost of swimming (p = 0.324) between diets. Heart rate did not differ between diets (p = 0.712), but oxygen pulse, a non-invasive surrogate for stroke volume, was greater following the HCLF diet (p = 0.029). CONCLUSIONS: A 3-day high-carbohydrate diet increased carbohydrate utilization but did not affect swimming economy at 50-70% VO2max. As these intensities are applicable to ultramarathon swims, future studies should use higher intensities that would be more relevant to shorter duration events.

Post-swim oxygen consumption: assessment methodologies and kinetics analysis.

OBJECTIVE: This study aimed at comparing different recovery-based methods to assess the highest exercise oxygen uptake value ([Formula: see text]O2peak) when swimming at low-moderate, heavy and severe intensities. Complementarily, the different recovery curve kinetics were analysed. APPROACH: Eighteen competitive swimmers performed a 5 × 200 m front crawl intermittent protocol (0.05 m · s-1 increments and 3 min intervals), with respiratory gas exchange being continuously measured breath-by-breath during and post-exercise using a portable gas analyser. The directly determined [Formula: see text]O2peak ([Formula: see text]O2dir) was compared with the values obtained by linear and exponential backward extrapolations (of different intervals) and the recovery curve mathematical modelling. MAIN RESULTS: [Formula: see text]O2dir rose with intensity increase: 41.96 ± 6.22, 46.36 ± 6.89 and 50.97 ± 7.28 ml · kg-1 min-1 for low-moderate, heavy and severe swims. Linear and exponential regressions applied to the first 20 s of recovery presented the [Formula: see text]O2peak values closest to [Formula: see text]O2dir at low-moderate (42.80 ± 5.54 vs 42.88 ± 5.58 ml kg-1 min-1), heavy (47.12 ± 4.91 vs 47.48 ± 5.09 ml kg-1 min-1) and severe intensity domains (51.24 ± 6.89 vs 53.60 ± 8.54 ml kg-1 · min-1, respectively; r = 0.5-0.8, p < 0.05). The mono-exponential function was the best fit at low-moderate and heavy intensities, while the bi-exponential function better characterized the severe exercise domain (with a slow component amplitude, time delay and time constant of 6.2 ± 2.3 ml kg-1 min-1, 116.6 ± 24.3 and 39.9 ± 15.2 s, respectively). SIGNIFICANCE: The backward extrapolation of the first 20 s of recovery is the best method to assess the [Formula: see text]O2peak for a large spectrum of swimming intensities. Complementarily, intensity increases imply different recovery curve kinetics, particularly a mono-exponential behaviour for low-moderate and heavy exertions and a bi-exponential dynamics for severe paces.

5 km front crawl in pool and open water swimming: breath-by-breath energy expenditure and kinematic analysis.

PURPOSE: Breath-by-breath energy expenditure during open water swimming has not yet been explored in an ecological environment. This study aimed to investigate and compare energetics and kinematics of 5 km swimming, in both swimming pool and open water conditions. METHODS: Through four independent studies, oxygen uptake ([Formula: see text]2) kinetics, heart rate (HR), blood lactate concentration ([La-]) and glucose level (BGL), metabolic power ([Formula: see text]), energy cost (C) and kinematics were assessed during 5 km front crawl trials in a swimming pool and open water conditions. A total of 38 competitive open water swimmers aged 16-27 years volunteered for this four part investigation: Study A (pool, ten females, 11 males), Study B (pool, four females, six males), Study C (pool case study, one female) and Study D (open water, three females, four males). RESULTS: In the swimming pool, swimmers started with an above average swimming speed (v), losing efficiency along the 5 km, despite apparent homeostasis for [La-], BGL, [Formula: see text]2, [Formula: see text] and C. In open water, swimmers started the 5 km with a below average v, increasing the stroke rate (SR) in the last 1000 m. In open water, [Formula: see text]2 kinetics parameters, HR, [La-], BGL, respiratory exchange ratio and C were affected by the v and SR fluctuations along the 5 km. CONCLUSIONS: Small fluctuations were observed for energetic variables in both conditions, but changes in C were lower in swimming pool than in open water. Coaches should adjust the training plan accordingly to the specificity of open water swimming.

The energy cost of swimming and its determinants.

The energy expended to transport the body over a given distance (C, the energy cost) increases with speed both on land and in water. At any given speed, C is lower on land (e.g., running or cycling) than in water (e.g., swimming or kayaking) and this difference can be easily understood when one considers that energy should be expended (among the others) to overcome resistive forces since these, at any given speed, are far larger in water (hydrodynamic resistance, drag) than on land (aerodynamic resistance). Another reason for the differences in C between water and land locomotion is the lower capability to exert useful forces in water than on land (e.g., a lower propelling efficiency in the former case). These two parameters (drag and efficiency) not only can explain the differences in C between land and water locomotion but can also explain the differences in C within a given form of locomotion (swimming at the surface, which is the topic of this review): e.g., differences between strokes or between swimmers of different age, sex, and technical level. In this review, the determinants of C (drag and efficiency, as well as energy expenditure in its aerobic and anaerobic components) will, thus, be described and discussed. In aquatic locomotion it is difficult to obtain quantitative measures of drag and efficiency and only a comprehensive (biophysical) approach could allow to understand which estimates are "reasonable" and which are not. Examples of these calculations are also reported and discussed.

Antidepressant and antinociceptive activities in animal models and in vitro assessment of the anti-thyroid activity of bis(DL-pyroglutamate)magnesium complex.

BACKGROUND: Magnesium is an essential element related with biochemistry of the brain and different types of depression have been associated with its deficiency. METHODS: The structure of a novel magnesium bis(DL-pyroglutamate) (Mg(DL-pGlu)2) was elucidated by X-ray crystallography. Wistar rats were used in the in vivo experiments. The antidepressant-like effect was assessed by the forced swim test (FST) and the antinociceptive activity was evaluated using hot plate test. In both, non-specific effects were evaluated by the open field test. Anti-thyroid activity was examined using Lang's method. Albumin binding behavior was evaluated by 3D fluorescence spectroscopy. RESULTS: For the Mg(DL-pGlu)2 complex (30 mg/kg), the FST test on Wistar rats revealed a decrease of 22% in the immobility time and an increment of 106% in the swimming time. The compound alters neither the locomotor activity nor the body weight after chronic administration. At the same dose, it showed antinociceptive activity, increasing the response latency. It blocks iodination reactions generating a charge transfer complex with iodine hence indicating anti-thyroid activity (Kc = 45366.5 ±â€¯29 M-1). Albumin 3D fluorescence spectroscopy experiments showed intensity increase of peak A and decrease of peak B. CONCLUSIONS: The results showed that the new compound produced a lowering of the immobility time and an increment of the swimming ability of the rats. The compound is able to increase the response latency in 70.0%, to capture iodine (anti-thyroid activity) and to interact with albumin through covalent type of interaction of the free NH groups.

Anaerobic capacity assessment in elite swimmers through inertial sensors.

OBJECTIVE: The present study aimed to assess if changes in speed and stroke parameters, as measured by an inertial sensor during a maximal effort swimming test, could provide an effective detection of anaerobic capacity in elite swimmers. APPROACH: Fourteen elite swimmers performed a 75 m maximal swimming test. Changes in speed and stroke parameters, estimated by a body-worn inertial sensor, were analysed to provide insight into stroke mechanics during swimming. Their relationships with the output of the Wingate Anaerobic Test were analysed. Best times in competition were also considered to assess swimmer's performance. MAIN RESULTS: Mean power measured using the Wingate cycle ergometer test highly correlated with mean speed attained by the swimmers during the proposed 75 m swimming test (R range: .700-.809, p  < .05). Mean power in the Wingate Anaerobic Test and mean speed in the 75 m swimming test highly correlated with best times attained by the swimmers (R range: .736-.855, p  < .01; R range: .659-.952, p  < .05, for Wingate and 75 m swimming test, respectively). Moreover, stroke variables were investigated: in this regard, a significant decrease in stroke rate and swimming speed and a significant increase in stroke length were observed between the first and the third lap (p  < .01). SIGNIFICANCE: The present in-water free swimming test provided insight into specific physiological/mechanical aspects of elite swimmers. The correlation of the swimming and the Wingate tests with swimmer's performance in competition confirms that they both reflect the skills and anaerobic qualities a swimmer uses in a race. The wearable inertial sensor could represent a feasible solution to evaluate stroke parameters, allowing a timely follow-up of variations in swimming biomechanics along the course of the test and the identification of differences in biomechanical strategy between swimmers. This analysis is of great interest for swimmers and coaches to characterise swimmer's technique weakness and strength, and to plan individual race pacing strategy.

Cost of transport is a repeatable trait but is not determined by mitochondrial efficiency in zebrafish (Danio rerio).

The energy used to move a given distance (cost of transport; CoT) varies significantly between individuals of the same species. A lower CoT allows animals to allocate more of their energy budget to growth and reproduction. A higher CoT may cause animals to adjust their movement across different environmental gradients to reduce energy allocated to movement. The aim of this project was to determine whether CoT is a repeatable trait within individuals, and to determine its physiological causes and ecological consequences. We found that CoT is a repeatable trait in zebrafish (Danio rerio). We rejected the hypothesis that mitochondrial efficiency (P/O ratios) predicted CoT. We also rejected the hypothesis that CoT is modulated by temperature acclimation, exercise training or their interaction, although CoT increased with increasing acute test temperature. There was a weak but significant negative correlation between CoT and dispersal, measured as the number of exploration decisions made by fish, and the distance travelled against the current in an artificial stream. However, CoT was not correlated with the voluntary speed of fish moving against the current. The implication of these results is that CoT reflects a fixed physiological phenotype of an individual, which is not plastic in response to persistent environmental changes. Consequently, individuals may have fundamentally different energy budgets as they move across environments, and may adjust movement patterns as a result of allocation trade-offs. It was surprising that mitochondrial efficiency did not explain differences in CoT, and our working hypothesis is that the energetics of muscle contraction and relaxation may determine CoT. The increase in CoT with increasing acute environmental temperature means that warming environments will increase the proportion of the energy budget allocated to locomotion unless individuals adjust their movement patterns.

Total energy expenditure in elite open-water swimmers.

This study aimed to examine the total energy expenditure (TEE) and physical activity level (PAL) of elite open-water swimmers. Our study group included 5 world-class competitive open-water swimmers. TEE was measured using the doubly labeled water method for 1 week. The TEE was 4549 ± 1185 kcal/day. The PAL was 3.22 ± 0.46. Our results may provide a reference to optimize energy requirement support.

The swimming plus-maze test: a novel high-throughput model for assessment of anxiety-related behaviour in larval and juvenile zebrafish (Danio rerio).

Larval zebrafish (Danio rerio) has the potential to supplement rodent models due to the availability of resource-efficient, high-throughput screening and high-resolution imaging techniques. Although behavioural models are available in larvae, only a few can be employed to assess anxiety. Here we present the swimming plus-maze (SPM) test paradigm, a tool to assess anxiety-related avoidance of shallow water bodies in early developmental stages. The "+" shaped apparatus consists of arms of different depth, representing different levels of aversiveness similarly to the rodent elevated plus-maze. The paradigm was validated (i) in larval and juvenile zebrafish, (ii) after administration of compounds affecting anxiety and (iii) in differentially aversive experimental conditions. Furthermore, we compared the SPM with conventional "anxiety tests" of zebrafish to identify their shared characteristics. We have clarified that the preference of deeper arms is ontogenetically conserved and can be abolished by anxiolytic or enhanced by anxiogenic agents, respectively. The behavioural readout is insensitive to environmental aversiveness and is unrelated to behaviours assessed by conventional tests involving young zebrafish. Taken together, we have developed a sensitive high-throughput test allowing the assessment of anxiety-related responses of zebrafish regardless of developmental stage, granting the opportunity to combine larva-based state-of-the-art methods with detailed behavioral analysis.

Isokinetic assessment of shoulder complex strength in adolescent elite synchronized swimmers.

Children and adolescent participation in sport has increased in recent years. Synchronized swimming requires correct muscle balance in the shoulder complex. The purpose of this study was to establish isokinetic strength profiles and peak torque ratios of shoulder internal and external rotator muscles in a female high-level synchronized swimming team. Twenty-six adolescent female high-level synchronized swimmers, aged 12-14, participated in this study. Maximal bilateral shoulder concentric external and internal rotation force was measured at 60°/s (5 repetitions) and 180°/s (15 repetitions). The isokinetic concentric strength generated by the internal rotator muscles was significantly higher (p < 0.05) than by the external rotators in both limbs and at both velocities. Significant bilateral differences in the external rotation (ER):internal rotation (IR) strength ratio were noted at 60°/s. Isokinetic assessment is essential in sports medicine, since it is the only test capable of diagnosing any shoulder strength deficit.