RESUMO
The minimal criteria for mesenchymal stem/stromal cell (MSC) identification set by the International Society for Cellular Therapy include plastic adherence, presence and absence of a set of surface antigens and in vitro multilineage differentiation. This differentiation is assessed through stimulation of MSCs with defined combination and concentration of growth factors towards specific lineages and histological confirmation of the presence of differentiated cells. Here we provide protocols for multilineage differentiation, namely, osteogenesis, adipogenesis, chondrogenesis and myogenesis. We also provide their respective histological analyses.
Assuntos
Adipócitos/citologia , Técnicas de Cultura de Células/métodos , Diferenciação Celular , Condrócitos/citologia , Células-Tronco Mesenquimais/citologia , Células Musculares/citologia , Osteócitos/citologia , Adipócitos/efeitos dos fármacos , Adipócitos/metabolismo , Adipogenia/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Linhagem da Célula/efeitos dos fármacos , Linhagem da Célula/fisiologia , Células Cultivadas , Condrócitos/efeitos dos fármacos , Condrócitos/metabolismo , Condrogênese/efeitos dos fármacos , Imunofluorescência , Humanos , Células-Tronco Mesenquimais/efeitos dos fármacos , Células Musculares/efeitos dos fármacos , Células Musculares/metabolismo , Desenvolvimento Muscular/efeitos dos fármacos , Músculo Esquelético/citologia , Osteócitos/efeitos dos fármacos , Osteócitos/metabolismo , Osteogênese/efeitos dos fármacos , Fluxo de TrabalhoRESUMO
Steroid-induced osteoporosis has been reported to show reduced osteoblastic bone formation and accelerated osteoclastic bone resorption. However, a fracture risk of patients with steroid-induced osteoporosis is higher than an assumed risk merely estimated by the bone mineral density, and therefore, bone quality appears to be reduced in the steroid-induced osteoporosis. Precious mechanism how steroid can reduce the quality of bone is still veiled, but one possible explanation may be osteocytes' apoptosis by steroid, probably resulting in the failure of their maintaining of bone minerals and mechanosensing in bone.