Removal of ochratoxin A in wine by Cryptococcus albidus and safety assessment of degradation products.

BACKGROUND: Ochratoxin A (OTA) is a mycotoxin that contaminates grape-based products and is extremely harmful to the health of the host. It is effectively removed by yeast during the fermentation of wine, whereas the removal mechanism of OTA remains unclear. Therefore, the present study aimed to investigate the removal mechanism of ochratoxin A by yeast and to evaluate the safety of its degradation products. RESULTS: Cryptococcus albidus (20-G) with better effect on ochratoxin A (OTA) was screened out in the main fermentation stage of wine. The results showed that 20-G removed OTA through biosorption and biodegradation. Intracellular enzymes played the main role (18.44%) and yeast cell walls adsorbed a small amount of OTA (8.44%). Furthermore, the identification of proteins in 20-G revealed that the decrease in OTA content was mainly a result of the action of peroxidase, and validation tests were carried out. By analyzing the degradation products of OTA, OTα and phenylalanine with lower toxicity were obtained. Animal experiments showed that the intervention of yeast 20-G reduced the damage and adverse effects caused by OTA toxicity to the mice. CONCLUSION: The present study demonstrates the mechanism of OTA removal by 20-G and the toxicity of OTA was reduced by peroxidase in 20-G. © 2023 Society of Chemical Industry.

Influence of elevated CO2 on nutritive value and health-promoting prospective of three genotypes of Alfalfa sprouts (Medicago Sativa).

Alfalfa sprouts are well known for their nutritive values. Although there are several studies reported the positive impact of elevated CO2 (eCO2) on plants, there are no in-depth, comprehensive studies on how eCO2 could improve the sprouting of plant seeds. Herein, the production of health-promoting metabolites was determined in eCO2 (620 ppm)-treated Alfalfa sprout cultivars (Giza 1, Nubaria and Ismailia 1). eCO2 increased the photosynthetic process and pigment contents, which consequently induced carbohydrates, proteins, fats and fiber accumulation. eCO2 also boosted the levels of vitamins, phenolics, flavonoids and mineral individuals and enhanced the antioxidant capacity of alfalfa sprouts. Interestingly, eCO2 reduced the antinutritional factor l-canavanine content in Ismailia 1 cultivar and improved the anti-inflammatory activities through inhibiting cyclooxygenase-2 and lipoxygenase activity. Therefore, eCO2 is a promising approach to improve the health-promoting prospective of alfalfa sprouts to be a valuable source of nutritious and bioactive compounds in our daily diet.

Fungal biodegradation and multi-level toxicity assessment of vinasse from distillation of winemaking by-products.

The wastewaters from distilleries of winemaking by-products, a scarcely studied type of vinasse, were treated by white-rot fungal strains from species Irpex lacteus, Ganoderma resinaceum, Trametes versicolor, Phlebia rufa and Bjerkandera adusta. The main objectives of this study were to evaluate fungal performance during vinasse biodegradation, their enzyme patterns and ecotoxicity evolution throughout treatment. Despite all strains were able to promote strong (>80%) dephenolization and reduction of total organic carbon (TOC), P. rufa was less affected by vinasse toxicity and exhibit better decolorization. In batch cultures at 28 °C and pH 4.0, the first phase of P. rufa biodegradation kinetics was characterized by strong metabolic activity with simultaneous depletion of TOC, phenolics and sugars. The main events of second phase are the increase of peroxidases production after the peak of laccase activity, and strong color removal. At the end of treatment, it was observed highly significant (p < 0.001) abatement of pollution parameters (83-100% removal). Since water reclamation and reuse for e.g. crop irrigation is a priority issue, vinasse ecotoxicity was assessed with bioindicators representing three different phylogenetic and trophic levels: a marine bacterium (Aliivibrio fischeri), a freshwater microcrustacean (Daphnia magna) and a dicotyledonous macrophyte (Lepidium sativum). It was observed significant (p < 0.05) reduction of initial vinasse toxicity, as evaluated by these bioindicators, deserving special mention an almost complete phytotoxicity elimination.

Toxicity evaluation and environmental risk assessment of 2-methyl-4-chlorophenoxy acetic acid (MCPA) on non-target aquatic macrophyte Hydrilla verticillata.

Aquatic plants in agricultural landscapes play a vital role in maintaining the ecological integrity within the aquatic systems while facing an array of disturbances. Among them, information on herbicide exposure on non-target aquatic plants is scarce. The present study was designed to fill this information gap by detecting the impacts of 2-methyl-4-chlorophenoxyacetic acid (MCPA) on Hydrilla verticillata using morpho-anatomical and physiological biomarkers and assessing the environmental risk of MCPA to the non-target environment. H. verticillata was exposed to different MCPA concentrations (10, 100, 500, 1000 µg/L) and control (0 µg/L) for 7 days. At the end of the experiment, plant growth, pigments, H2O2 content, peroxidase activity (POD) and plant anatomy were compared. The environmental risk was assessed using predicted environmental concentration/predicted no effect concentration (PEC:PNEC) ratio, hazard quotient (HQ) and hazard index (HI). Control plants exhibited the highest growth, and a growth decline was noted in parallel to MCPA exposure, where a similar trend was detected for the plant pigment contents. MCPA induced chlorosis and oxidative stress in H. verticillata. Risk analysis detected high values for PEC:PNEC ratios (3-9), HQ (1.92-5.79) and HI (28.15). MCPA-exposed H. verticillata could recover once those plants received natural conditions. Overall, present findings showed the negative impacts of MCPA on non-target aquatic plant H. verticillata. These findings will be useful to clarify the interaction between agrochemicals and non-target aquatic plants. Such information would benefit to decide the criteria in aquatic ecosystem management.

A colorimetric assay of DNA methyltransferase activity based on peroxidase mimicking of DNA template Ag/Pt bimetallic nanoclusters.

DNA methylation catalyzed by DNA methyl transferase (MTase) is a significant epigenetic process for modulating gene expression. Abnormal levels of DNA MTase enzyme have been regarded as a cancer biomarker or a sign of bacterial diseases. We developed a novel colorimetric method to assay M.SssI MTase activity employing peroxidase-like activity of DNA template Ag/Pt NCs without using restriction enzymes. Based on inhibiting the peroxidase reaction that occurred in the TMB-H2O2 system, in the presence of MTase, a highly sensitive and selective colorimetric biosensor was fabricated with a detection limit (LOD) of 0.05 U/mL and a linear range from 0.5 to 10 U/mL. The changes in absorption intensity were monitored to quantify the M.SssI activity. This strategy had a high selectivity over other proteins. Furthermore, it is also demonstrated that this method can be used for the evaluation and screening of inhibitors for DNA MTase.

Genotoxicity assessment of pulp and paper mill effluent before and after bacterial degradation using Allium cepa test.

A lignin peroxidases-producing Serratia liquefaciens was used for bioremediation of pulp and paper (P&P) mill effluent. The treatment led to reduction of chemical oxygen demand (COD), colour, lignin and phenolic content by 84%, 72%, 61% and 95%, respectively. The effluent detoxification was studied by genotoxicity assays using Allium cepa L. (onion) root tip cells. Genotoxicity studies included measuring mitotic index (MI), chromosomal aberrations (CA) and nuclear abnormalities (NA) in root tip cells following treatment with 25, 50, 75 and 100% (v/v) of effluent. The root tip cells grown in untreated effluent showed a significant decrease in MI from 69% (control) to 32%, 27%, 22% and 11% at 25%, 50%, 75% and 100% effluent concentration, respectively. This indicated that the untreated effluent was highly cytotoxic in nature. Further, root tip cells, when treated with different concentrations of effluent showed various CA and NA including c-mitosis, stickiness, chromosome loss, chromosome break, anaphase bridge, multipolar anaphase, vagrant chromosomes, micronucleated and binucleated cells. The MI observed in root tip cells grown in bacterial treated effluents at similar concentrations (25, 50, 75 and 100% v/v) showed an increase of 33%, 36%, 42% and 66%. CA showed a substantial decrease and in some instances, complete absence of CA was also observed. The findings suggest that S. liquefaciens culture could be a potential bacterial culture for bioremediation of P&P mill effluent, as it is effective in substantial lowering of pollutants load as well as reduces the cytotoxic and genotoxic effects of effluent.

In vitro assessment of physiological changes of watermelon (Citrullus lanatus) upon iron oxide nanoparticles exposure.

With the rapid development of nanotechnology, developing nano iron fertilizer is an important strategy to alleviate Fe deficiency and elevate Fe fertilization effect in agricultural applications. In this study, watermelon seedlings were grown in soil amended with iron oxide nanoparticles (γ-Fe2O3 NPs) at different concentrations (0, 20, 50, 100 mg/L). The content of soluble sugar and protein, content of chlorophyll and malondialdehyde (MDA), and activity of antioxidant enzymes of watermelon leaves were determined in five successive weeks to evaluate the physiological changes of watermelon plants after γ-Fe2O3 NPs exposure. Transmission electron microscope (TEM) observations indicated that γ-Fe2O3 NPs could enter root cell of watermelon. Results showed that 20 mg/L γ-Fe2O3 NPs didn't cause any oxidative stress on watermelon and 50 mg/L γ-Fe2O3 NPs could increase soluble sugar, soluble protein and chlorophyll content in the growth of plants. In addition, 50 and 100 mg/L γ-Fe2O3 NPs caused oxidative stress on watermelon leaves, but this NP-induced stress was removed with the growth of watermelon. It is noteworthy that we found γ-Fe2O3 NPs might possess an intrinsic peroxidase-like activity. The variation trend of physiological parameters was correlated with the nutritional requirements of plants. It can be concluded that γ-Fe2O3 NPs at proper concentrations have the ability to improve iron deficiency chlorosis and promote the growth of watermelon plants. To the best of the author's knowledge, this is the first holistic study focusing on the impact of γ-Fe2O3 NPs in long-term experiment of watermelon plants.

Assessment of cadmium accumulation, toxicity, and tolerance in Brassicaceae and Fabaceae plants--implications for phytoremediation.

This study, based on a greenhouse pot culture experiment conducted with 15-day-old rapeseed (Brassica campestris L. cv. Pusa Gold; family Brassicaceae) and moong bean (Vigna radiata L. Wilczek cv. Pusa Ratna; family Fabaceae) plants treated with cadmium (Cd) concentrations (0, 50, and 100 mg kg(-1) soil), investigates their potential for Cd accumulation and tolerance, and dissects the underlying basic physiological/biochemical mechanisms. In both species, plant dry mass decreased, while Cd concentration of both root and shoot increased with increase in soil Cd. Roots harbored a higher amount of Cd (vs. shoot) in B. campestris, while the reverse applied to V. radiata. By comparison, root Cd concentration was higher in B. campestris than in V. radiata. The high Cd concentrations in B. campestris roots and V. radiata shoots led to significant elevation in oxidative indices, as measured in terms of electrolyte leakage, H2O2 content, and lipid peroxidation. Both plants displayed differential adaptation strategies to counteract the Cd burden-caused anomalies in their roots and shoots. In B. campestris, increasing Cd burden led to a significantly decreased reduced glutathione (GSH) content but a significant increase in activities of GSH reductase (GR), GSH peroxidase (GPX), and GSH sulfotransferase (GST). However, in V. radiata, increasing Cd burden caused significant increase in GSH content and GR activity, but a significant decline in activities of GPX and GST. Cross talks on Cd burden of tissues and the adapted Cd tolerance strategies against Cd burden-accrued toxicity indicated that B. campestris and V. radiata are good Cd stabilizer and Cd extractor, respectively, wherein a fine tuning among the major components (GR, GPX, GST, GSH) of the GSH redox system helped the plants to counteract differentially the Cd load-induced anomalies in tissues. On the whole, the physiological/biochemical characterization of the B. campestris and V. radiata responses to varying Cd concentrations can be of great help in elaborating the innovative plant-based remediation technologies for metal/metalloid-contaminated sites.

Assessment of toxic effects of triclosan on the terrestrial snail (Achatina fulica).

Triclosan (TCS) is a broad-spectrum antimicrobial agent used in personal care products, and as a result, is widespread in the environment. Toxicity tests of TCS on aquatic organisms have been reported, but limited toxicity data on terrestrial species are available. In this study, the 28-d chronic toxicity of TCS on the biomass, shell diameter growth, and total food intake of the terrestrial snail Achatina fulica were tested. Moreover, biochemical responses, including changes in the activity of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and the content of malondialdehyde (MDA), were examined after 14-d and 28-d exposure. Results showed that TCS had toxic effects on the biomass, shell diameter growth, and total food intake of A. fulica with no observed effect concentration (NOEC) values of 24 mg kg(-1). As for the antioxidant enzymes, TCS caused significant oxidative stress even at the low concentration of 24 mg kg(-1). The CAT and POD activities at the high concentrations of 200 and 340 mg kg(-1), respectively, were significantly inhibited. The SOD and CAT activity in treatments below 118 mg kg(-1) and the MDA content in all treatments showed dose-effect relationships. This study demonstrated that TCS caused adverse effects on terrestrial invertebrates, and provided valuable information for the risk assessment imposed by TCS in the terrestrial environment.

Asparagus byproducts as a new source of peroxidases.

Soluble peroxidase (POD) from asparagus byproducts was purified by ion exchange chromatographies, and its kinetic and catalytic properties were studied. The isoelectric point of the purified isoperoxidases was 9.1, and the optimum pH and temperature values were 4.0 and 25 °C, respectively. The cationic asparagus POD (CAP) midpoint inactivation temperature was 57 °C, which favors its use in industrial processes. The Km values of cationic asparagus POD for H2O2 and ABTS were 0.318 and 0.634 mM, respectively. The purified CAP is economically obtained from raw materials using a simple protocol and possesses features that make it advantageous for the potential use of this enzyme in a large number of processes with demonstrated requirements of thermostable POD. The results indicate that CAP can be used as a potential candidate for removing phenolic contaminants.