Pesticide application behavior in green tea cultivation and risk assessment of tea products: a case study of Rizhao green tea.

Previous research on pesticides in green tea mainly focused on detection technology but lacked insights into pesticide use during cultivation. To address this gap, a survey was conducted among Rizhao green tea farmers. The survey results showed that most tea farmers were approximately 60 years old and managed small, scattered tea gardens (< 0.067 ha). Notably, tea farmers who had received agricultural training executed more standardized pesticide application practices. Matrine and thiazinone are the most used pesticides. A total of 16 types of pesticides were detected in the tested green tea samples, with 65% of the samples containing residues of at least one pesticide. Notably, higher levels of residues were observed for bifenthrin, cyfluthrin, and acetamiprid. The presence of pesticide residues varied significantly between seasons and regions. The risk assessment results indicated that the hazard quotient (HQ) values for all 16 pesticides detected in green tea were < 1, suggesting that these residue levels do not pose a significant public health concern.

Spatial distribution and risk assessment of pyrethroid insecticides in surface waters of East China Sea estuaries.

Pyrethroid insecticides are the most commonly used household insecticides and pose substantial risks to marine aquatic organisms. many studies have detected pyrethroid insecticides in the waters and estuaries of the western United States, but their distributions within western Pacific estuaries have not been reported. Accordingly, we used high-throughput organic analyses combined with high volume solid-phase extraction to comprehensively assess 13 pyrethroid insecticides in East China Sea estuaries and the Huangpu River. The results demonstrated the presence of various ∑13pyrethroid insecticides in East China Sea estuaries (mean and median values of 8.45 ± 5.57 and 7.78 ng L-1, respectively), among which cypermethrin was the primary contaminant. The concentrations of ∑12pyrethroid insecticide detected in the surface waters at the Huangpu River (mean 6.7 ng L-1, outlet 16.4 ng L-1) were higher than those in the Shanghai estuary (4.7 ng L-1), suggesting that runoff from inland areas is a notable source of insecticides. Wetlands reduced the amount of runoff containing pyrethroid insecticides that reached the ocean. Several factors influenced pesticide distributions in East China Sea estuaries, and higher proportions were derived from agricultural sources than from urban sources, with a higher proportion of agricultural sources than urban sources, influenced by anthropogenic use in the region. Permethrin and cypermethrin were the main compounds contributing to the high ecological risk in the estuaries. Consequently, to prevent risks to marine aquatic life, policymakers should aim to reduce insecticide contaminants derived from urban and agricultural sources.

In vivo-in vitro correlations (IVIVC) for the assessment of pyrethroid bioavailability in honey.

Bioaccessibility/bioavailability is an important factor in assessing the potential human health risk via oral exposure. However, methods for accurately predicting the bioaccessibility/bioavailability of pesticide residues are still limited, preventing accurate measurements of actual exposure to pesticide residues. In this study, pyrethroid bioavailability in honey were analysed using a mouse bioassay and bioaccessibility via in vitro methods with Tenax extraction. The results demonstrated that the combined liver plus kidney data served as an appropriate biomarker to estimate the relative bioavailability. Notably, significant in vivo-in vitro correlations (IVIVC) were observed between bioavailability and bioaccessibility (R2 = 0.7898-0.9793). Estimation of the bioavailability of honey from different nectar plants using derived IVIVC confirmed that different contents and physicochemical properties might affect its bioavailability. The findings provide insight into assessing human exposure to pesticides based on bioavailability and can decrease the uncertainty about the assessment of the risk of dietary exposure to pesticides.

Dietary exposure risk assessment of pyrethroids in fruits and vegetables: a national scale investigation.

The pyrethroids (PYRs) were extensively used to increase agriculture outputs. However, the cumulative exposures of PYRs would bring about potential risks through food intake. It is an urgent requirement to explore the cumulative exposures on the fruits and vegetables. In this study, a total of 1720 samples incorporating eight primary fruits and vegetables collected around China were investigated to assess the health risk for adults and children from eight PYRs. The relative potency factor (RPF) method was employed to reveal both chronic and acute cumulative exposure. As a result, the hazard index (HI) were 0.004 ~ 0.200% and 11.85 ~ 99.19% for chronic and acute cumulative dietary exposure, respectively. The national wide investigation indicated the cumulative assessments were not hazardous. Besides, the acute intake of pear, grape, and lettuce should be paid on more attention, particularly. This study provides compelling evidence to develop relative policy and regulation to improve the food quality and safety.

Pesticide Exposure among Latinx Children in Rural Farmworker and Urban Non-Farmworker Communities: Associations with Locality and Season.

This study uses repeated measures to document the pesticide exposure of rural and urban Latinx children (age eight at baseline), and to compare these children in terms of the frequency and concentration of their exposure to a large set of pesticides, accounting for season. We used silicone wristbands worn for one week up to ten times at quarterly intervals from 2018 to 2022 to assess pesticide exposure in children from rural farmworker (n = 75) and urban non-farmworker (n = 61) families. We determined the detection and concentrations (ng/g) of 72 pesticides and pesticide degradation products in the wristbands using gas chromatography electron capture detection and gas chromatography mass spectrometry. The most frequently detected pesticide classes were organochlorines, pyrethroids, and organophosphates. Controlling for season, organochlorine or phenylpyrazole detections were less likely for rural children than for urban children. Detections of organochlorines, pyrethroids, or organophosphates were lower in spring and summer versus winter. Controlling for season, urban children had greater concentrations of organochlorines, while rural children had greater concentrations of pyrethroids and Chlorpyrifos. Pesticide concentrations were lower in winter and spring compared with summer and fall. These results further document that pesticides are ubiquitous in the living environment for children in vulnerable, immigrant communities.

Assessment of Preschool Children's Exposure Levels to Organophosphate and Pyrethroid Pesticide: A Human Biomonitoring Study in Two Turkish Provinces.

Pesticides are products developed to prevent, destroy, repel or control certain forms of plant or animal life that are considered to be pests. However, now they are one of the critical risk factors threatening the environment, and they create a significant threat to the health of children. Organophosphate (OP) and pyrethroid (PYR) pesticides are widely used in Turkey as well as all over the world. The main focus of this presented study was to analyze the OP and PYR exposure levels in urine samples obtained from 3- to 6-year-old Turkish preschool children who live in the Ankara (n:132) and Mersin (n:54) provinces. In order to measure the concentrations of three nonspecific metabolites of PYR insecticides and four nonspecific and one specific metabolite of OPs, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses were performed. The nonspecific PYR metabolite 3-phenoxybenzoic acid (3-PBA) found in 87.1% of samples (n = 162) and the specific OP metabolite 3,5,6-trichloro-2-pyridinol (TCPY) found in 60.2% of samples (n = 112) were the most frequently detected metabolites in all urine samples. The mean concentrations of 3-PBA and TCPY were 0.38 ± 0.8 and 0.11 ± 0.43 ng/g creatinine, respectively. Although due to the large individual variation no statistically significant differences were found between 3-PBA (p = 0.9969) and TCPY (p = 0.6558) urine levels in the two provinces, significant exposure differences were determined both between provinces and within the province in terms of gender. Risk assessment strategies performed in light of our findings do not disclose any proof of a possible health problems related to analyzed pesticide exposure in Turkish children.

Residual Behavior and Dietary Risk Assessment of Chlorfenapyr and Its Metabolites in Radish.

Chlorfenapyr, as a highly effective and low-toxicity insect growth regulation inhibitor, has been used to control cross-cruciferous vegetable pests. However, the pesticide residue caused by its application threatens human health. In this paper, the residue digestion and final residue of chlorfenapyr in radish were studied in a field experiment. The results of the dynamic digestion test showed that the half-life of chlorfenapyr in radish leaves ranged from 6.0 to 6.4 days, and the digestion rate was fast. The median residual values of chlorfenapyr in radish and radish leaves at 14 days after treatment were 0.12 and 3.92 mg/kg, respectively. The results of the dietary intake risk assessment showed that the national estimated daily intake (NEDI) of chlorfenapyr in various populations in China were 0.373 and 5.66 µg/(kg bw·d), respectively. The risk entropy (RQ) was 0.012 and 0.147, respectively, indicating that the chronic dietary intake risk of chlorfenapyr in radish was low. The results of this study provided data support and a theoretical basis for guiding the scientific use of chlorfenapyr in radish production and evaluating the dietary risk of chlorfenapyr in vegetables.

Effect of bifenthrin application at different maturity stages on its dissipation and residues in kumquat (Citrus japonica) and dietary intake risk assessment.

Bifenthrin is a pyrethroid pesticide widely used on kumquats, but the residues in the peel and pulp after bifenthrin application at different maturity stages of kumquats have not been evaluated. This study developed a simple and rapid high-performance liquid chromatography (HPLC) method for the quantitative analysis of bifenthrin residues in whole fruit, kumquat peel, kumquat pulp, and soil. The results showed that regardless of whether bifenthrin was applied one or three times during the near-mature period, the half-lives of the fruit peel and fruit pulp were longer than those in the immature period. Kumquat fruit residues decreased with time at both maturity levels. The residues of bifenthrin in near-mature fruit exceeded the MRL in Guangxi and Fujian 14 days after the three applications of bifenthrin, suggesting that this issue should be focused on in kumquat production and supervision. However, for bifenthrin application in either the near-mature or the immature fruit period, the calculated risks for chronic dietary intake of kumquat were well below 100%. The data demonstrate that the chronic dietary intake risk of bifenthrin through kumquat consumption is low and within acceptable limits. These results provide a reference and risk assessment data for the safe and rational use of bifenthrin insecticides.

Pyrethroids contamination and health risk assessment in seafood collected from the coast of Shandong, China.

This study provides the first data on pyrethroid residues of seafood in China. A total of 192 seafood samples were randomly selected from four coastal cities of Shandong Province in 2020. The residues of fenpropathrin, cypermethrin and deltamethrin in seafood by GC-MS were ND to 26.82 µg/kg, ND to 19.18 µg/kg and ND to 15.56 µg/kg, respectively. The cumulative risk to general population of different age groups was assessed by the hazard quotient (HQ) and hazard index (HI) approaches, and showed that the maximum value of both HQ (1.81 × 10-3) and HI (2.9 × 10-3) were below the threshold 1. The present results indicated that the three main pyrethroids from the consumption of seafood is unlikely to pose a health risk to general populations in Shandong. The uncertainty analysis indicated that the monitoring study of pyrethroid residues in seafood is worthy of continuous attention to ensure food safety.

Residual levels and dietary risk assessment of bifenthrin and dinotefuran and its major metabolites in open wheat field conditions.

To evaluate the residual levels of bifenthrin and dinotefuran, a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) and high-performance liquid chromatography-tandem mass spectrometry method for simultaneous detection of bifenthrin and dinotefuran and its major metabolites in wheat was developed and validated. Dietary risk assessments were further performed based on the relevant residual data from 12 wheat fields, toxicology data and dietary patterns. In wheat grain and straw, the recoveries of all analytes ranged from 77 to 102% with the relative standard deviation <9.7% and the limit of quantitation 0.05 mg kg-1 . The highest terminal residue of bifenthrin in wheat grain was 0.069 mg kg-1 and dinotefuran was 0.34 mg kg-1 . Residual concentrations of bifenthrin and dinotefuran decreased to <0.05 and 0.15 mg kg-1 at 21 days (pre-harvest interval), respectively. The chronic risk quotient ranged from 6.4 to 62.7% and the acute risk quotient varied from 0.38 to 17.73%. The chronic and acute dietary risks caused by the terminal residues of the two insecticides were negligible for Chinese populations. The recommended pre-harvest interval was proposed to ensure safe wheat consumption. These data could provide a scientific reference to establish the Chinese maximum residue limit of dinotefuran in wheat.

Occurrence of pyrethroids in the atmosphere of urban areas of Southeastern Brazil: Inhalation exposure and health risk assessment.

The occurrence of organochlorine pesticides (OCPs) used decades ago for vector control in urban areas is still reported as a threat to human health. Pyrethroids emerged as a replacement for OCPs in sanitary campaigns and are currently the main insecticides used for vector control worldwide, with prominent use as agricultural and household insecticides, for veterinary and gardening purposes, and as wood preservative. This study aimed to assess the occurrence, seasonal variation, and potential sources of pyrethroids in ambient air of two urban regions of Southeastern Brazil, along with the potential health risks to local populations via inhalation exposure. Pyrethroids were sampled by polyurethane foam passive air samplers and their concentrations were determined by gas chromatography coupled with electron capture negative ionization mass spectrometry (GC/ECNI-MS). Atmospheric pyrethroid concentrations (hereinafter reported in pg m-3) were considerably higher than those reported by previous studies worldwide. Cypermethrin (median: 2446; range: 461-15 125) and permethrin (655; 19-10 328) accounted for 95% of the total measured pyrethroids in ambient air. The remaining fraction comprised smaller amounts of bifenthrin (46;  urban-industrial > background areas. HQs increased with decreasing age group, but deterministic and probabilistic estimates did not identify direct health risks for any group. Nevertheless, since only inhalation exposure was considered in this work, other pathways should be investigated to provide a more comprehensive risk assessment of the human exposure to pyrethroids.

Dissipation kinetics and consumer risk assessment of novaluron + lambda-cyhalothrin co-formulation in cabbage.

Cabbage, one of the most popular vegetables in the world is infested by several insect-pests and diseases. Novaluron, a chitin synthesis inhibitor and lambda-cyhalothrin, a synthetic pyrethroid group insecticide are used to manage insect-pests on cabbage. The dissipation kinetics and risk assessment of combination formulation (novaluron 9.45% + lambda-cyhalothrin 1.9%) with different modes of action has not yet been investigated in cabbage. Multi-location supervised field trials were therefore, conducted in different agro-climatic regions of India for safety evaluation of the combination product. The co-formulation at the recommended (novaluron 750 g a.i. ha-1 + lambda-cyhalothrin 750 g a.i. ha-1) and double the recommended (novaluron 1500 g a.i. ha-1 + lambda-cyhalothrin 1500 g a.i. ha-1) dose was sprayed on the cabbage crop. The samples were extracted and cleaned up using a modified QuEChERS method, and the residues analyzed by GC-ECD and GC-MS. The half-life (t1/2) varied between 1.77 and 2.51 and 2.00-3.38 days for novaluron and 1.36-2.24 and 1.69-3.82 days for lambda-cyhalothrin in cabbage at respective doses. The Food Safety and Standards Authority of India (FSSAI) has set the MRL of 0.7 mg kg-1 for novaluron at PHI of 5 days, and no MRL is set for lambda-cyhalothrin in cabbage. On the basis of OECD MRL calculator, the MRLs of 0.6 and 1.5 mg kg-1 for novaluron and lambda-cyhalothrin, respectively were calculated at the respective doses at PHI of 3 days. Hazard quotient (HQ) <1, theoretical maximum daily intake (TMDI) < acceptable daily intake (ADI) and < maximum permissible intake (MPI), percent acute hazard index (% aHI) <1, and percent chronic hazard index (% cHI) <1 for both novaluron and lambda-cyhalothrin suggested that the combination formulation is safe and will not pose any dietary risk to the consumers. The study will be helpful to conduct risk assessment of other pesticides/combination pesticides on food crops on which their MRLs have not yet been fixed.

Residual determination of pyrethrins in Lycium barbarum (goji) by GC-MS/MS and a dietary risk assessment of Chinese goji consumption.

An accurate and simple analytical approach for the determination of residues cinerin I, cinerin II, jasmolin I, jasmolin II, pyrethrin I and pyrethrin II (six active ingredients of pyrethrins) in fresh and dried goji berries was developed and validated for analysis by gas chromatography with tandem mass spectrometry. Good linearity (determination coefficient >0.99), accuracy (average recoveries of 88.3%-111.5%) and precision (intra- and inter-day relative standard deviations of 0.4%-8.3%) were obtained with the optimised determination method. The LODs and LOQs of the six analytes in two goji matrices were 0.24-2.1 µg/kg and 0.8-7 µg/kg, respectively. In a field trial, the terminal residual levels of pyrethrins (the sum of the concentrations of the six target analytes) in fresh and dried goji berry samples were <20-304 µg/kg at harvest, which could provide some information for the establishment of a maximum residue limit of pyrethrins on goji berries in China. Moreover, the risk assessment results indicated that because the risk quotient values were ≪100%, the potential dietary risk of pyrethrins on goji berries could be negligible for Chinese consumers. These detection and field results could provide some supporting data for the determination of pyrethrin residues in other crops and the proper application and safety assessment of pyrethrins in goji plants.

Dietary intake assessment of pyrethroid residues from okra and eggplant grown in peri-urban areas of Punjab, Pakistan.

This study was designed to assess the gradual increase in the use of insecticides on vegetables and to familiarize the consumers regarding the insecticide residues. The purpose of this research work was to highlight the detrimental effects of pyrethroids (bifenthrin, cyfluthrin, cypermethrin, deltamethrin, fenvalerate, lambda-cyhalothrin, and permethrin) compare with dietary intake assessment of eggplant and okra grown in peri-urban environment. In this manner, a total of 180 (n = 60 × 3) samples of eggplant (Solanum melongena) and okra (Abelmoschus esculentus) were procured from the peri-urban farming system of Faisalabad, Multan, and Gujranwala to assess the pyrethroid residues along with their dietary intake assessment. The procured vegetables were quantified for pyrethroid residues by using gas chromatography (GC) equipped with an electron capture detector (ECD). Outcomes of this study revealed that for okra samples, the highest residues of bifenthrin (1.25 mg kg-1) were found in Gujranwala then Multan (1.5 mg kg-1) and Faisalabad (1.04 mg kg-1), whereas in eggplant, the highest residues were recorded for bifenthrin from Faisalabad (1.33 mg kg-1) and Gujranwala (0.78 mg kg-1). In Multan, the highest residues for cyfluthrin (1.18 mg kg-1) were reported in eggplant. Out of all analyzed samples for pyrethroid residues, 32% samples contained detectable residues and 6% samples exceeded their maximum residual limits (MRLs) established by the European Union (EU). Dietary intake assessment (mg kg-1 day-1) was calculated as per their maximum permissible intake (MPI) values, i.e., bifenthrin (1.28), cyfluthrin (1.28), cypermethrin (3.20), deltamethrin (0.64), fenvalerate (1.28), lambda-cyhalothrin (0.064), and permethrin (3.20) respectively. Conclusively, residues from the Multan region were greater than those from Gujranwala and Faisalabad showing excessive application of pyrethroids. Overall results revealed that although some samples exceeded MRLs in selected areas, their safe consumption limit was found.

An assessment of pesticide exposures and land use of honey bees in Virginia.

Large-scale honey bee colony loss threatens pollination services throughout the United States. An increase in anthropogenic pressure may influence the exposure of hives to household and agricultural pesticides. The objective of this survey was to provide an assessment of the risk of exposure to commonly used pesticides to honey bee colonies in Virginia in relation to land use. Adult honey bee, pollen, and wax samples from colonies throughout Virginia were evaluated for pyrethroid, organophosphate, organochlorine, and triazine pesticides using gas chromatography-mass spectrometry analysis. Of the 11 pesticides analyzed, nine were detected in one or more hive matrices. The probability of detecting a pesticide in pollen was less in forests than in pasture, agriculture, or urban landscapes. Coumaphos and fluvalinate were significantly more likely to be detected across all matrices with concentrations in wax as high as 15500 and 6970 ng/g (dry weight), respectively, indicating the need for further research on the potential effects of miticide accumulation in wax to larval and adult bees.

Pesticide residues in Lebanese apples and health risk assessment.

In Lebanon, apples are among the most consumed commodities; however, pesticide residues in apples have not been evaluated so far. Therefore, this study presents the occurrence of pesticide residues in apples produced in Lebanon during 2012-2016. A total of 212 samples were analysed for the presence of pesticides using the QuEChERS multi-residue extraction method, followed by GC-MS. Pesticide residues were not observed in 23% of the samples. Whereas pesticide residues were found in 77% of the samples, among them 61% exceeded the maximum residue limits (MRL) and 16% contained residues below the MRL. The most frequently detected pesticide residues were chlorpyrifos (n = 142), methidathion (n = 34), cypermethrin (n = 21), lambda-cyhalothrin (n = 16), myclobutanil (n = 13) and diazinon (n = 9). A preliminary long-term exposure assessment for the detected pesticides in apples showed that Hazard Quotient (HQ) was in the range of 0.1-8% of the ADI's, indicating no risk to human health.

Dissipation behavior and dietary risk assessment of lambda-cyhalothrin, thiamethoxam and its metabolite clothianidin in apple after open field application.

The dissipation dynamics and residue amounts of lambda-cyhalothrin, thiamethoxam and clothianidin in apple were investigated by using rapid resolution liquid chromatography triple quadrupole mass spectrometer (RRLC-MS/MS) and gas chromatography mass spectrometry (GC-MS). The developed method performed satisfactory recoveries of 88%-105% and the limit of quantitation (LOQ) was 0.01 mg kg-1. The suspension concentrate (SC) formulation of lambda-cyhalothrin and thiamethoxam was applied on apple field in accordance with good agricultural practice (GAP). The half-lives of two pesticides ranged from 7.01 d to 17.3 d and the terminal residues were <0.01-0.21 mg kg-1. Based on the Chinese dietary pattern, the dietary risk of lambda-cyhalothrin and total thiamethoxam were predicted by comparing intake amounts with the toxicological data, namely acceptable daily intake (ADI) and acute reference dose (ARfD). The chronic and acute risk quotients were 0.1080-0.4463 and 0.0008-0.2005, respectively, which showed negligible risk for general consumers. The pre-harvest interval (PHI) of 21 d was suggested for the formulation in compliance with maximum residue limit (MRL) and dietary risk assessment, meanwhile, the MRL of 0.1 mg kg-1 was recommended for thiamethoxam in apple. These results were vital for guiding reasonable usage of two insecticides and for approval of formulation use.

Daily Intake Estimation for Young Children's Ingestion of Residential Dust and Soils Contaminated with Chlorpyrifos and Cypermethrin in Taiwan.

We estimated the daily intakes of chlorpyrifos and cypermethrin via ingestion of indoor dust and outdoor soils using the Stochastic Human Exposure and Dose Simulation Model on a probabilistic approach for Taiwanese young children. Variables for the estimation, such as concentration, ingestion rate, and body weight, were adopted from previous studies. Monte Carlo simulation was performed with 1,000,000 iterations to simulate a single daily intake, which was shown in terms of percentage of the Acceptable Daily Intake (ADI) of either insecticide. The daily intakes are minimal with a 99% probability, but go up steeply at the 99.9th percentile (13.1% and 20.0% of the ADIs of chlorpyrifos and cypermethrin, respectively). The sensitivity analysis indicates that concentration is the most determinant variable for daily intake estimation, suggesting that high intakes may occur when insecticide concentrations are elevated. Compared to the data of daily intakes via dietary ingestion of vegetables derived from a previous study, the estimated non-dietary intakes are negligible until reaching the highest percentile. Consequently, the non-dietary ingestion exposure to either insecticide is commonly low for young children in Taiwan’s homes, unless high contamination (e.g., indoor insecticide application) occurs in the environment. Care has to be taken to avoid high contamination indoors.

In vitro assessment of pyrethroid bioaccessibility via particle ingestion.

Due to their intensive use in agricultural and residential pest control, human exposure to residues of multiple pyrethroids frequently occurs. Pyrethroids have exceptionally high affinity for solid particles, highlighting the need to understand human exposure through oral ingestion of contaminated soil or dust particles. In this study, we used artificial gastrointestinal fluids to measure the desorption or bioaccessibility of eight current-use pyrethroids in soil and dust samples. Tenax was further included as a sink in parallel treatments to simulate the effect of removal due to transfer of pyrethroids to lipid membranes. The use of 0.4 g of Tenax in 20 mL digestive fluids resulted in rapid and efficient trapping of pyrethroids, and further, greatly increased bioaccessibility. In the artificial digestive fluids without Tenax, 6.0-48.0% of pyrethroids were desorbed over 21 h, and the fractions increased by 1.6-4.1 folds to 21.5-79.3% with the Tenax sink. Therefore, 6.0-79.3% of soil or dust-borne pyrethroids may be considered bioavailable upon ingestion. While protein and sucrose increased the estimated bioaccessibility, co-presence of lipid (vegetable oil) decreased the bioaccessibility of pyrethroids, likely due to competitive phase partition. Pyrethroids were also found to be unstable in the artificial intestinal fluid containing pancreatin, further decreasing the potential bioaccessibility of pyrethroids on soil or dust particles. The limited bioaccessibility should be considered to refine the prediction of human exposure and risk through oral ingestion of pyrethroid residues.

Impact of a proposed revision of the IESTI equation on the acute risk assessment conducted when setting maximum residue levels (MRLs) in the European Union (EU): A case study.

Proposals to update the methodology for the international estimated short-term intake (IESTI) equations were made during an international workshop held in Geneva in 2015. Changes to several parameters of the current four IESTI equations (cases 1, 2a, 2b, and 3) were proposed. In this study, the overall impact of these proposed changes on estimates of short-term exposure was studied using the large portion data available in the European Food Safety Authority PRIMo model and the residue data submitted in the framework of the European Maximum Residue Levels (MRL) review under Article 12 of Regulation (EC) No 396/2005. Evaluation of consumer exposure using the current and proposed equations resulted in substantial differences in the exposure estimates; however, there were no significant changes regarding the number of accepted MRLs. For the different IESTI cases, the median ratio of the new versus the current equation is 1.1 for case 1, 1.4 for case 2a, 0.75 for case 2b, and 1 for case 3. The impact, expressed as a shift in the IESTI distribution profile, indicated that the 95th percentile IESTI shifted from 50% of the acute reference dose (ARfD) with the current equations to 65% of the ARfD with the proposed equations. This IESTI increase resulted in the loss of 1.2% of the MRLs (37 out of 3110) tested within this study. At the same time, the proposed equations would have allowed 0.4% of the MRLs (14 out of 3110) that were rejected with the current equations to be accepted. The commodity groups that were most impacted by these modifications are solanacea (e.g., potato, eggplant), lettuces, pulses (dry), leafy brassica (e.g., kale, Chinese cabbage), and pome fruits. The active substances that were most affected were fluazifop-p-butyl, deltamethrin, and lambda-cyhalothrin.