Assessment of human health risk concerning edible plants contamination with toxic elements around functional and derelict mines.

Edible plants samples were analysed for non-carcinogenic and carcinogenic human health risks. The elements nickel (Ni), cadmium (Cd), arsenic (As), lead (Pb), chromium (Cr) and mercury (Hg) analysed using atomic absorption spectrophotometer (AAS). The recovery, limit of detection (LOD) and limit of quantification (LOQ) ranged from 75 to 89 %, 0.001-0.003 and 0.003-0.01, respectively. The mean value of Hg (0.34 mg/kg) exceeded the limit of 0.05 mg/kg recommended by World Health Organization (WHO). The estimated daily intake (EDI) of Cd in adults ranged from 7.93 × 10-7 to 1.43 × 10-4 and that of Hg from 0.07 to 1.27 and Cd (0.08 × 10-4) in children. These are below the oral reference doses (RfD). Hazard Quotient (HQ) of Hg in children was 1.92. The elements Hg and As obtained the highest total hazard (THI) index of 2.02 in mango1 and oil palm. Arsenic possessed the highest cancer risk of 4.5 × 10-4 in children and 1.9 × 10-4 in adults. Cancer risk (CR) ranged from low to moderate (10-6-10-4), which is below the limit of 10-3. The total carcinogenic risk (TCR) of the edible plants were above the limit of 10-6. The study identified minimal As and Hg pollution and carcinogenic risks in edible plants.

The pan-plastome of Hemerocallis citrina reveals new insights into the genetic diversity and cultivation history of an economically important food plant.

BACKGROUND: Hemerocallis citrina Baroni (Huang hua cai in Chinese) is a perennial herbaceous plant grown for its flower buds that are eaten fresh or dried and is known as the vegetarian three treasures. The nuclear genome of H. citrina has been reported, but the intraspecific variation of the plastome (plastid genome) has not yet been studied. Therefore, the panplastome of this species collected from diverse locations is reported here for the first time. RESULTS: In this study, 65 H. citrina samples were resequenced, de novo assembled, and aligned with the published plastome of H. citrina to resolve the H. citrina panplastome. The sizes of the 65 newly assembled complete plastomes of H. citrina ranged from 156,048 bp to 156,263 bp, and the total GC content ranged from 37.31 to 37.34%. The structure of the complete plastomes showed a typical tetrameric structure, including a large single copy (LSC), a small single copy (SSC), and a pair of inverted repeat regions (IRA and IRB). Many nucleotide variants were identified between plastomes, among which the variants in the intergenic spacer region were the most abundant, with the highest number of variants concentrated in the LSC region. Based on the phylogenetic tree constructed using the ML method, population structure analysis, and principal component analysis (PCA), the panplastome data were subdivided into five genetic clusters. The C5 genetic cluster was mostly represented by samples from Qidong, Hunan Province, while samples from Shanxi and Shaanxi Provinces were classified into the C4 genetic cluster. The greatest genetic diversity was found in the C1 genetic cluster, and the greatest genetic distance between any two clusters was found between the C4 and C5 clusters. CONCLUSION: The resolution of the panplastome and the analysis of the population structure of H. citrina plastomes provide important data for future breeding projects and germplasm preservation.

Food plants in Brazil: origin, economic value of pollination and pollinator shortage risk.

Pollination is a key ecosystem service of critical importance for food production. However, globally, several regions are already experiencing pollinator shortage as pollinators are declining. Here, we investigate the origin, pollinator dependence and economic value of 199 food crops cultivated in Brazil to understand to which extent (1) Brazilian agriculture is vulnerable to pollinator shortage, and (2) Brazilian society has already achieved a comprehensive perspective about crop dependence. We used Brazil as a case study as it is a megadiverse tropical country and the 3rd largest world crop producer and exporter, with most of the crops depending on pollinators. Our findings revealed that over half (53.7%) of the food crops in Brazil are native, with the North region of Brazil housing the higher diversity of native crops, in contrast with the South and Southeast regions. Additionally, considering the reproductive systems, among native food crops, 65.6% exhibit self-incompatibility or dioecy (i.e., requiring obligatory cross-pollination), whereas 30.6% of exotic food crops display this trait. Overall, Brazilian municipalities produce more exotic crops than native ones, with almost 4/5 of the total agricultural area of the country dedicated to the cultivation of exotic crops, which are generally self-compatible commodities that rely low to modestly on pollinators. Regarding the biomes, we observe that this pattern is followed by most of them, but for the Caatinga dry forest, where native crops dependent on pollinators predominate. However, when soybean is removed from the analysis, the areas devoted to exotic crops always decreased, even being equal to native crops in the Atlantic forest. Our results also indicate that considering the pollinator shortage, some Brazilian biomes may be at risk of losing >20% of their yields, mainly in the Caatinga dry forest and the Atlantic forest. Therefore, in this paper, we are discussing that the expansion of monocultures in Brazil's agricultural lands may have several impacts on the provision of pollination services, food production and, then, on food security not only for the Brazilian population, as Brazil is the 3rd largest world agricultural producer and exporter.

Biodiversity is overlooked in the diets of different social groups in Brazil.

Food biodiversity is essential for improving nutrition and reducing hunger in populations worldwide. However, in middle and low-income countries, the biodiversity of food production does not necessarily represent food consumption patterns by population. We used Brazil, one of the world's megabiodiverse countries, as a case study to investigate the following questions: what is the prevalence of consumption of biodiverse foods in Brazil, and what are the socioeconomic factors that influence their consumption throughout the country? We used data from a Brazilian representative national dietary survey to estimate the frequency of food consumption of unconventional food plants, edible mushrooms, and wild meat, in according to socioeconomic variables. Thus, we investigated the socioeconomic predictors of Unconventional Food Plants consumption using methods of Machine Learning (ML) and multiple zero-inflated Poisson (ZIP) regression. We showed that biodiverse food consumption in Brazil is low, just related by 1.3% of the population, varying in according to area, ethnicity, age, food insecurity, sex, and educational level. Our findings of low utilization of biodiversity suggest an important mismatch between the rich biodiversity of the country and its representation in the human diet.

Enhanced edible plant production using nano-manganese and nano-iron fertilizers: Current status, detection methods and risk assessment.

BACKGROUND: Nanotechnology offers many benefits in the globally important field of food production and human nutrition, particularly by implementing agricultural nanoproducts. Of these, edible plant fertilizers enriched with nanosized forms of essential metals, Mn and Fe, are growing in importance with the advantages of enhanced action on plant roots. SCOPE AND APPROACH: This review focuses on the importance of tracking the bioaccumulation and biodistribution of these pertinent nanofertilizers. An emphasis is given to the critical analysis of the state-of-the-art analytical strategies to examine the Mn and Fe nanoparticles in edible plant systems as well as to shedding light on the vast gap in the methodologies dedicated to the speciation, in vitro simulation, and safety testing of these promising nanomaterials. Also provided are guidances for the food chemists and technologists on the lights and shadows of particular analytical approaches as a matter of authors' expertise as analytical chemists. KEY FINDINGS AND CONCLUSIONS: While the use of nanotechnology in agriculture seems to be growing increasingly, there is still a lack of analytical methodologies capable of investigating novel Mn- and Fe-based nanomaterials as potential fertilizers. Only the advent of reliable analytical tools in the field could bridge the gaps in our knowledge about processes in which those materials participate in the plant systems and their effects on crop production and quality of the produced food.

Contamination and Health Risk Assessment of Multiple Mycotoxins in Edible and Medicinal Plants.

Edible and medicinal plants (EMPs) are widely used but are easily infected by harmful fungi which produce mycotoxins. Herein, 127 samples from 11 provinces were collected to investigate 15 mycotoxins based on geographic, demographic, processing, and risk characteristics. A total of 13 mycotoxins were detected, and aflatoxin B1 (0.56~97.00 µg/kg), deoxynivalenol (9.41~1570.35 µg/kg), fumonisin B1 (8.25~1875.77 µg/kg), fumonisin B2 (2.74~543.01 µg/kg), ochratoxin A (0.62~19.30 µg/kg), and zearalenone (1.64~2376.58 µg/kg) occurred more frequently. Mycotoxin levels and species were significantly different by region, types of EMPs, and method of processing. The margin of exposure (MOE) values was well below the safe MOE (10,000). AFB1 exposure from Coix seed and malt consumption in China was of high health concern. The hazard Index (HI) method showed the range of 113.15~130.73% for malt, indicating a public health concern. In conclusion, EMPs should be concerned because of the cumulative effects of co-occurred mycotoxins, and safety management strategies should be developed in follow-up studies.

A Quantitative Study on the Ethnobotanical Knowledge about Wild Edible Plants among the Population of Messiwa.

Background: The preservation of traditional knowledge of wild edible plants (WEPs) is one of the challenges to the sustainability of natural resources. Therefore, it is crucial to assess the traditional knowledge of WEPs in relation to some socio-demographic and economic factors. Methods: The survey was conducted among the Messiwapopulationthrough a semi-structured questionnaire. The first part of the questionnaire concerns socio-demographic and economic information, while the second part concerns the plants recognized and used by the informant. The recognition frequency (RF), the use frequency (UF), the general consumption frequency(CF), the recent consumption frequency (RCF), and the correlation between these frequencies were evaluated. A comparison of means was also used to compare informant's knowledge according to their socio-demographic and economic status. Results: The three species;Foeniculum vulgare, Ziziphus lotus, and Malva sylvestris were the most recognized (FR = 1) and the most used (FU = 1). The consumption frequency (CF) and the recent consumption frequency (RCF) for Foeniculum vulgare were 1 and 0.9, respectively. Taraxacum getulum, Calendula arvensis and Cyperus rotundus were the least recognized (FR= 0.16; 0.16; 0.48) and least used (FU = 0.3; 0.3; 0.4) species, respectively. The informants who showed a high level of traditional knowledge on WEPs were housewives, with a low level of schooling and at least 45 years old. Conclusion: Despite the decline in traditional knowledge about wild edible plants, some populations preserve this knowledge, especially among the elderly. Therefore, documentation of this knowledge is necessary through ethnobotanical and ethnomedicinal studies.

Ethnobotanical study of the wild edible and healthy functional plant resources of the Gelao people in northern Guizhou, China.

INTRODUCTION: The Gelao people are a unique minority in Southwest China with a unique culture for the utilization of edible plants, including a large number of medicinal plants. They believe that at least 61 species are edible and have medicinal value. Ethnobotany research can reveal the local knowledge of the Gelao people regarding the traditional use of plants and the relationship between this minority and their living environment to help retain and pass on this traditional knowledge forever. METHODS: Edible wild plants and their applied ethnic knowledge were investigated in three counties in northern Guizhou. Gelao residents were the main informants, and literature search, village interviews, participatory observation and quantitative ethnobotany evaluation were used. RESULTS: A total of 151 species of wild plants in 67 families are collected and eaten by Gelao residents, among which 61 species were considered to have medicinal value, accounting for 40.4% of the total, and 43 were listed in the Chinese Pharmacopoeia. There were 57 plant species with fruits as their edible parts, which are consumed as snacks, followed by 54 species whose young seedlings and leaves are the edible parts, most of which are consumed cold or stir-fried. Other edible parts included roots or rhizomes (bulbs), flowers, whole plants, seeds, fruiting bodies and stems. There were two consumption modes: raw and cooked. Raw foods were mainly consumed as snacks, which mainly comprise fruits. Cooked foods were mainly vegetables consumed cold or stir-fried. Some plants were used as seasonings, infused wines, condiments and grains. The main medicinal functions were nourishing and reducing heatiness. Nourishing plants were mainly "shen" plants and Liliaceae, while plants able to reduce heatiness were mainly Asteraceae. Others functions included anti-hangover, anticancer and insecticidal. There were 38 species of important edible wild plants (CFSI > 500) in northern Guizhou, which had a high utilization rate. Houttuynia cordata Thunb. and Mentha suaveolens Ehrh. were the most representative edible wild plants in this area. The species, edible parts, edible categories, consumption modes and medicinal functions of edible wild plants in this area are diverse, and the traditional knowledge on their uses is rich. However, the number of wild plant species eaten by the informants and their related knowledge were positively correlated with age, which indicates that the rich traditional knowledge in this area is gradually disappearing with urbanization. CONCLUSIONS: The Gelao have a rich history of consuming wild plants. With the development of the social economy, the traditional knowledge passed from older generations is gradually being lost and its inheritance is facing great risks. This study collects, sorts and spreads this precious traditional knowledge, which is of great value to its protection and inheritance and fully demonstrates the value and importance of our work.

An ethnobotanical study on wild plants used by Tibetan people in Gyirong Valley, Tibet, China.

BACKGROUND: Gyirong Valley known as the "Back Garden of the Himalayas" is located in the core area of the Everest National Nature Reserve. It is also one of the important ports from ancient Tibet to Kathmandu, Nepal, since ancient times. Over the years, the Tibetans of Gyirong had accumulated sufficient traditional knowledge about local plant resources. However, there is almost no comprehensive report available on ethnobotanical knowledge about the local people. The purposes of this study were to (1) conduct a comprehensive study of wild plants used by Tibetan people in Gyirong Valley and record the traditional knowledge associated with wild useful plants, (2) explore the influence of Tibetan traditional culture and economic development on the use of wild plants by local people, and (3) explore the characteristics of traditional knowledge about wild plants of Tibetans in Gyirong. METHODS: Ethnobotanical data were documented through free listings, key informant interviews and semi-structured interviews during fieldwork. The culture importance index and the informant consensus factor index were used as quantitative indices. RESULTS: In total, 120 informants (61 women and 59 men) and 3333 use reports and 111 wild plant species belonging to 39 families and 81 genera were included. These use reports were then classified into 27 categories belonging to three major categories. The use category that contained the most plant species was edible plants (62), followed by medicinal plants (32) and economic plants (22), and other uses (71). Plants with high CI included Allium prattii, Neopicrorhiza scrophulariiflora, Gymnadenia orchidis, Rhododendron anthopogon and Fritillaria cirrhosa. Thirty-six species of plants in the catalog of Gyirong and Yadong were the same, but only 17 species were the same in Gyirong and Burang. There were only 11 overlapping species between all the three regions. CONCLUSION: Tibetans of Gyirong have rich and unique knowledge about plant use, and wild edible and medicinal plants play an important role in the nutrition and health protection of local people. However, traditional knowledge is slowly being lost and is being hit by modern tourism. In the future, more attention needs to be paid to the important role of traditional knowledge in biodiversity conservation.

Accumulation and risk assessment of antibiotics in edible plants grown in contaminated farmlands: A review.

The extensive occurrence of antibiotics in farmland soil might threaten food safety. The bioaccumulation potential of antibiotics in edible vegetables and crops grown under realistic farming scenarios was reviewed and the human health risk was assessed. A total of 51 antibiotics were documented in 37 species of daily consumed crops. Among different classes of antibiotics, tetracyclines (TCs) exhibited higher residue levels in plants than quinolones (QNs), sulfonamides (SAs), and macrolides (MLs), with median values ranging from 5.10 to 15.4 µg/kg dry weight. The favored accumulation of TCs in plants was probably linked to their relatively higher residual concentrations in soils and greater bioconcentration factors. Compared with the plants grown in open field, accumulation of antibiotics was higher in plant grown under greenhouse condition, probably due to the higher residue levels of antibiotics in the greenhouse soil with intensive application of manure. Cocktails of antibiotics were investigated in potato, corn, carrot, tomato, lettuce, and wheat. Among them, corn exhibited relatively high median concentrations of antibiotics (0.400-203 µg/kg dry weight). Antibiotics tended to accumulate in plant root and their concentrations in fruit were generally low. Risk assessment revealed that human health risk was under the alert line through the daily consumption of antibiotic contaminated vegetables and food crops.

Socioeconomic factors influencing knowledge and consumption of food plants by a human group in a mountainous environment in the semiarid region of Bahia, Northeast Brazil.

BACKGROUND: The relationship of people with natural resources is guided by different sociocultural, ecological and evolutionary factors. Regarding food plants, it is not different. Studies around the world have evaluated the effects of socioeconomic factors, such as age, gender, income, profession, education level, time of residence, ethnic diversity, religion, festive rituals, access to urban areas and migrations. In this sense, the objective of the present study was to characterize the diversity of knowledge and use of food plants by people from Serra dos Morgados and evaluate if the socioeconomic factors influence knowledge and consumption of food plants in the community. METHODOLOGY: This research was conducted in the village of Serra dos Morgados, municipality of Jaguarari, Bahia, with the purpose of evaluating the factors that influence in the knowledge and use of food plants. Socioeconomic data such as age, gender, time of residence, and monthly income were collected. The free list technique was applied during the collection of ethnobotanical data in order to analyze the preference of the plants based on the salience index (SI). To analyze the factors that influence knowledge and use forms, we used GLM Lasso. RESULTS: A total of 33 people were interviewed, 8 men and 25 women; their age ranged from 30 to 82 years. People cited 98 species of plants, 41 species being identified of spontaneous occurrence. The plant with the highest salience index (SI) was "cheirosa" (Psidium ganevii) (SI = 0.5679), followed by "massaranduba" (Micropholis sp.) (SI = 0.4323); "araça" (Campomanesia guazumifolia) (SI = 0.3320); and "cambuí" (Siphoneugena sp.) (SI = 0.3144). CONCLUSIONS: The main factors that influence knowledge and use forms in the locality were family income and the collection site, with homegardens cited as the preferred area for collection of food plants. This study provided an overview related to potentially important species for a community located in a region where there are few ethnobiological studies. The results presented here can be used in future studies, providing clues for investigations. Also, there is a contribution to the conservation of biocultural aspects related to the use of food plants in a community living in mountainous regions.

Growing up in the Betsileo landscape: Children's wild edible plants knowledge in Madagascar.

Understanding local knowledge about wild edible plants (WEP) is essential for assessing plant services, reducing the risks of knowledge extinction, recognizing the rights of local communities, and improving biodiversity conservation efforts. However, the knowledge of specific groups such as women or children tends to be under-represented in local ecological knowledge (LEK) research. In this study, we explore how knowledge of WEP is distributed across gender and life stages (adults/children) among Betsileo people in the southern highlands of Madagascar. Using data from free listings with 42 adults and 40 children, gender-balanced, we show that knowledge on WEP differs widely across gender and life stage. In addition, we find that children have extended knowledge of WEP while reporting different species than adults. Women's knowledge specializes in herbaceous species (versus other plant life forms), while men's knowledge specializes in endemic species (versus native or introduced). Finally, we find that introduced species are more frequently cited by children, while adults cite more endemic species. We discuss the LEK differentiation mechanisms and the implications of acquiring life stage's knowledge in the highland landscapes of Madagascar. Given our findings, we highlight the importance of considering groups with under-represented knowledge repositories, such as children and women, into future research.

Macronutrients, trace metals and health risk assessment in agricultural soil and edible plants of Mahshahr City, Iran.

In this study, we evaluate the geochemistry of macro- (Fe, P, Ca, S, K, Na, Mg) and micronutrients (Mo, Cu, Pb, Zn, Co, Mn, Cd, Sr, Cr, Hg, Se), along with possible health risks of heavy metals contamination in agricultural soils and vegetables of the Mahshahr industrial port in Iran. Calculation of geochemical coefficients revealed the low pollution load of Mahshahr agricultural soils. Most of the investigated elements exhibited lower concentrations in soil than international standards. Element concentrations in plant samples were far below the permissible values set by environmental agencies. Based on permissible values, there was deficiency of several soil elements, including Cu, in vegetables because they are mostly present in the soil residual phase. An exception was Mn, which is the most mobile element in soil. The transfer factor (TF) of elements showed the following trend: K > Na > P > S > Mo > Hg > Se > Zn > Cd > Cu > Mg > Mn > Ca > Cr > Co ≈ Fe = Pb. There was high transfer of major elements from soil to plants, and lower values for micronutrients and heavy metals. The calculated daily intake (DIM) and health risk index (HRI) for ten plant species for adults were < 1, while the HRI was larger than 1 for Mn via radish consumption. According to geochemical investigations and statistical tests such as principal component analysis, Kruskal-Wallis, and correlation coefficient calculations, plant species play the most important role in elemental uptake by plants.

RIFM fragrance ingredient safety assessment, ß-caryophyllene, CAS Registry Number 87-44-5.

The existing information supports the use of this material as described in this safety assessment. ß-Caryophyllene was evaluated for genotoxicity, repeated dose toxicity, reproductive toxicity, local respiratory toxicity, phototoxicity/photoallergenicity, skin sensitization, and environmental safety. Data show that ß-caryophyllene is not genotoxic. Data on ß-caryophyllene provided a calculated Margin of Exposure (MOE) > 100 for the repeated dose toxicity and fertility endpoints. The developmental and local respiratory toxicity endpoints were evaluated using the Threshold of Toxicological Concern (TTC) for a Cramer Class I material, and the exposure to ß-caryophyllene is below the TTC (0.03 mg/kg/day and 1.4 mg/day, respectively. Data show that there are no safety concerns for ß-caryophyllene for skin sensitization under the current declared levels of use. The phototoxicity/photoallergenicity endpoints were evaluated based on data and ultraviolet/visible (UV/Vis) spectra; ß-caryophyllene is not expected to be phototoxic/photoallergenic. The environmental endpoints were evaluated; ß-caryophyllene was found not to be Persistent, Bioaccumulative, and Toxic (PBT) as per the International Fragrance Association (IFRA) Environmental Standards, and its risk quotients, based on its current volume of use in Europe and North America (i.e., Predicted Environmental Concentration/Predicted No Effect Concentration [PEC/PNEC]), are <1.

Uptake of potentially toxic elements by edible plants in experimental mining Technosols: preliminary assessment.

A study was carried out to evaluate the absorption of potentially toxic elements from mining Technosols by three types of vegetable plants (broccoli (Brassica oleracea var. italica), lettuce (Lactuca sativa) and onion (Allium cepa)), the different parts of which are intended for human and farm animal consumption (leaves, roots, edible parts). The preliminary results obtained highlight the importance of the design of the mining Technosols used for agricultural purposes, obtained from soils and sediments of mining origin and amended with residues of high calcium carbonate concentrations (limestone filler and construction and demolition wastes). The experiment was carried out in a greenhouse, and the total metal(loid)s concentration (As, Pb, Cd, Cu, Fe, Mn and Zn) of the soil, rhizosphere, aqueous leachates and plant samples was monitored, the translocation and bioconcentration factors (TF and BCF, respectively) being calculated. The characterization of the soils included a mobilization study in media simulating different environmental conditions that can affect these soils and predicting the differences in behavior of each Technosol. The results obtained showed that the levels of potentially toxic elements present in the cultivated species are within the range of values mentioned in the literature when they were cultivated in soils with calcareous amendments. However, when the plants were grown in contaminated soils, the potentially toxic elements levels varied greatly according to the species, being higher in onions than in lettuce. Experiments with the use of lime filler or construction and demolition wastes for soil remediation result in crops that, in principle, do not present health risks and are similar in development to those grown on non-contaminated soil.

Ximenia americana: Economic Importance, Medicinal Value, and Current Status in Ethiopia.

Ximenia americana is one of the most valuable wild edible plants in the world. In different countries, it is utilized as food, medicine, an essential oil source, and the industrial component to other products. In Ethiopia, it was one of the most known and very important plants for a long period of time. It was utilized as food, a medicinal plant, and animal feed. It was also one of the most economically important and culturally valuable plants. But nowadays, it is not adequately available in the country due to deforestation problem in the years. In addition, its economic importance, current status, and medicinal roles are not well documented and understood. As for research studies, it is concluded that unless a collective effort is taken, the existence of this plant is under severe threat and needs to have some measures. This review article is aimed at addressing the abovelined topics in detail and to pinpoint and explain the importance and status of Ximenia americana.

Assessment of potentially reusable edible wild plant and mushroom gathering sites in eastern Fukushima based on external radiation dose.

Edible wild plant/mushroom gathering, an essential food acquisition and outdoor recreation activity in rural areas, has declined in the area near the Fukushima Dai-ichi Nuclear Power Plant (FDNPP) accident in eastern Japan. The present study first evaluated the spatial distribution of potential gathering sites of various edible wild plant/mushroom species before the accident by administering a face-to-face questionnaire survey to local gatherers as well as utilizing the group analytical hierarchy process (AHP) and geographic information systems (GIS). Then, the damage to and future reusability of previous gathering sites were estimated from the perspective of the external radiation dose by overlaying maps of potential gathering sites and the time-series air dose rate (ADR) up to 2050 incorporating different gathering frequency scenarios. The study area is located in Kawauchi village in the eastern Fukushima prefecture, at 12-30 km southwest of FDNPP. The spatial distributions of gathering sites before the accident differed widely among species at the local scale because of their different environmental preferences. In contrast, the temporal variation in the reusability of the gathering sites was notably small among different species. The external radiation dose in the potential gathering sites declined sharply in the early post-accident years and gradually in the later years through the physical decay of radiocesium, i.e., 134Cs and 137Cs, with different half-lives. Moreover, the gathering frequency and heterogeneous distribution of radiocesium substantially affected the temporal variation in reusability for the gathering sites. These results indicate that an early resumption of gathering would be possible by reducing the gathering frequency and avoiding higher ADR areas, whereas it will take a much longer period to resume gathering in areas with higher ADR. Further research is required that considers both internal and external radiation doses in the geospatial context for the restoration and safer use of edible wild plants/mushrooms.

Rapid Ethnonutrition Assessment Method Is Useful to Prototype Dietary Assessments with a Focus on Local Biodiverse Food Plants.

Ethnonutrition is the study of diets in the context of food systems of different peoples and cultures. Its scope comprises native or local categories used to classify food, and also includes biodiverse food availability, local culinary techniques, seasonality, and cultural perceptions related to diet with nutritional implications. Here, we describe a method useful for gathering ethnonutrition data to design dietary interventions or assessments, the Rapid Ethnonutrition Assessment (REA). REA is a tool that offers food and nutrition research a broad biocultural view of diets, considering food system-level, by prototyping dietary assessments with high efficiency. This method permits us to prevent misinterpretations that lead to wrong conclusions in nutritional research.

Valorisation of kitul, an overlooked food plant: Phenolic profiling of fruits and inflorescences and assessment of their effects on diabetes-related targets.

Caryota urens L. has long been valued as a traditional food, the edible fruits being eaten raw and the inflorescences commonly used on sweet sap and flour production. In the current work, the phenolic profile of methanol extracts obtained from the inflorescences and fruits was unveiled for the first time, nine caffeic acid derivatives being identified and quantified. Since kitul products have been reported for their antidiabetic properties, extracts radical scavenging activity and α-amylase, α-glucosidase and aldose reductase inhibitory activity were assessed. The inflorescences' extract was particularly active against yeast α-glucosidase (IC50 = 1.53 µg/mL), acting through a non-competitive inhibitory mechanism. This activity was also observed in enzyme-enriched homogenates obtained from human Caco-2 cells (IC50 = 64.75 µg/mL). Additionally, the extract obtained from the inflorescences showed no cytotoxicity on HepG2, AGS and Caco-2 cell lines. Our data suggest that C. urens inflorescences can support the development of new functional foods with α-glucosidase inhibitory activity.

Culinary and nutritional value of edible wild plants from northern Spain rich in phenolic compounds with potential health benefits.

Wild edible plants (WEPs) have been consumed since ancient times. They are considered as non-domesticated plants that grow spontaneously in nature, particularly in forests and bushlands, where they can be found and collected to be incorporated into human nutrition. Increasingly, WEPs are gaining importance as they are potential sources of food due to their nutritional value, besides showing positive health effects and offer innovative applications in haute cuisine. As these autochthonous plants grow naturally in the environment, they are more suitable to adapt to different climatic conditions as well as biotic and abiotic factors. Therefore, a door has been opened for their possible cultivation as they seem to require fewer expenses than other commercially cultivated plants. Moreover, the consumers demand for new products of natural origin that are sustainable and ecologically labeled have also boosted WEPs' recovery and incorporation into the market. In addition, they are considered as promising sources of essential compounds needed not only in human diet including carbohydrates, proteins, and lipids but also of other minor compounds as phenols, vitamins, or carotenoids that have shown numerous beneficial bioactivities such as antioxidants, anti-inflammatory, or anti-tumor activity. The use of these plants rich in bioactive molecules could be beneficial from the health point of view as the human body is not always capable of producing enough defenses, for instance, preventing oxidative damage. In particular, the presence of phenolic compounds in these vegetal matrices is supposed to provide a prophylactic effect against further pathogenesis and disorders related to aging or oxidative stresses. Regarding all this information based on traditional knowledge and ethnobotanical data, different WEPs found in the Northwestern region of Spain were selected, namely, Mentha suaveolens, Glechoma hederacea, Prunus spinosa, Apium nodiflorum, Artemisia absinthium, Silybum marianum, Picris hieracioides, Portulaca oleracea, Crithmum maritimum, and the genus Amaranthus. However, even though tradition and popular knowledge are excellent tools for the exploitation of these plants, it is necessary to develop regulations in this aspect to assure safety and veracity of food products. This article aims to review the main aspects of their bioactive properties, their traditional use, and the possibility of their incorporation into the market as new functional foods, looking at innovative and healthy gastronomic applications.