A sandwich-type photoelectrochemical biosensor based on Ru(bpy)32+ sensitized In2S3 for aflatoxin B1 detection.

Aflatoxin B1 (AFB1), classified as a class I carcinogen, is a widespread mycotoxin that poses a serious threat to public health and economic development, and the food safety problems caused by AFB1 have aroused worldwide concern. The development of accurate and sensitive methods for the detection of AFB1 is significant for food safety monitoring. In this work, a sandwich-type photoelectrochemical (PEC) biosensor for AFB1 detection was constructed on the basis of an aptamer-antibody structure. A good photocurrent response was obtained due to the sensitization of In2S3 by Ru(bpy)32+. In addition, this sandwich-type sensor constructed by modification with the antibody, target detector, and aptamer layer by layer attenuated the migration hindering effect of photogenerated carriers caused by the double antibody structure. The aptamer and antibody synergistically recognized and captured the target analyte, resulting in more reliable PEC response signals. CdSe@CdS QDs-Apt were modified as a signal-off probe onto the sensor platform to quantitatively detect AFB1 with a "signal-off" response, which enhanced the sensitivity of the sensor. The PEC biosensor showed a linear response range from 10-12 to 10-6 g mL-1 with a detection limit of 0.023 pg mL-1, providing a feasible approach for the quantitative detection of AFB1 in food samples.

Circular economical approach of extracting nanocarbons from waste pea peel for sensing of p-nitrophenol and its conversion into paracetamol.

An important paradigm shift towards the circular economy is to prioritize waste prevention, reuse, recycling, and recovery before disposal is necessary. In this context, a sustainable protocol of converting waste pea peel (wPP) into low-cost carbon nanomaterials for sensing and conversion of p-nitrophenol (p-NP) into value-added paracetamol is being reported. Two fractions of the carbonaceous nanomaterials were obtained after the hydrothermal treatment (HT) of wPP, firstly an aqueous portion containing water-soluble carbon dots (wPP-CDs) and a solid residue, which was converted into carbonized biochar (wPP-BC). Blue-colored fluorescent wPP-CDs displayed excitation-dependent and pH-independent properties with a quantum yield (QY) of 8.82 %, which were exploited for the fluorescence sensing of p-NP with 4.20 µM limit of detection. Pyrolyzed biochar acting as an efficient catalyst effectively reduces p-NP to p-aminophenol (p-AP) in just 16 min with a 0.237 min-1 rate of conversion. Furthermore, the produced p-AP was converted into paracetamol, an analgesic and antipyretic drug, to achieve zero waste theory. Thus, this study provides the execution of sustainable approaches based on the integral valorization of biowaste that can be further recycled and reused, offering an effective way to attain a profitable circular economy.

Recent Advances of carbon Pathways for Sustainable Environment development.

Carbon dots (CDs) are an emerging type of carbon nanomaterial with strong biocompatibility, distinct chemical and physical properties, and low toxicity. CDs may emit fluorescence in the ultraviolet (UV) to near-infrared (NIR) range, which renders them beneficial for biomedical applications. CDs are usually made from carbon precursors and can be synthesized using top-down and bottom-up methods and it can be easily functionalized using different methods. For specific cases of biomedical applications carbon dot functionalization augments the materials' characteristics. Novel functionalization techniques are still being investigated. This review will look at the benefits of functionalization to attain a high yield and various biological applications. Biomedical applications such as photodynamic and photothermal therapy, biosensing, bioimaging, and antiviral and antibacterial properties will be covered in this review. The future applications of green synthesized carbon dots will be determined in part by this review.

Strategic Assessment of Boron-Enriched Carbon Dots/Naproxen: Diagnostic, Toxicity, and In Vivo Therapeutic Evaluation.

Cancer is a significant global public health concern, ranking as the leading cause of mortality worldwide. This study thoroughly explores boron-doped carbon dots (B-CDs) through a simple/rapid microwave-assisted approach and their versatile applications in cancer therapy. The result was highly uniform particles with an average diameter of approximately 4 nm. B-CDs exhibited notable properties, including strong fluorescence with a quantum yield of 33%. Colloid stability tests revealed their robustness within a pH range of 6-12, NaCl concentrations up to 0.5 M, and temperatures ranging from 30 to 60 °C. The study also delved into the kinetics of naproxen release from B-CDs as a drug delivery system. The loading efficacy of naproxen exceeded 55.56%. Under varying pH conditions, the release of naproxen from B-CDs conformed to the Peppas-Sahlin model, demonstrating the potential of Naproxen-loaded CDs for cancer drug delivery. In vitro cytotoxicity assessments, conducted using the CCK-8 Assay and flow cytometry, consistently indicated low toxicity with average cell viability exceeding 80%. An in vivo toxicity test on female mice administered 20 mg/kg of B-CDs for 31 days revealed reversible histological changes in the liver and kidneys, while the pancreas remained unaffected. Importantly, B-CDs did not impact the mice's physical behavior, body weight, or survival. In vivo experiments targeting benzo(a)pyrene-induced fibrosarcoma demonstrated the efficacy of B-CDs as naproxen carriers in the treatment of cancer. This in vivo study provides a thorough comprehension of B-CDs synthesis and toxicity and their potential applications in cancer therapy and drug delivery systems.

Assessment of biomass-derived carbon dots as highly sensitive and selective templates for the sensing of hazardous ions.

Access to safe drinking water and a hygienic living environment are the basic necessities that encourage healthy living. However, the presence of various pollutants (especially toxic heavy metal ions) at high concentrations in water renders water unfit for drinking and domestic use. The presence of high concentrations of heavy-metal ions (e.g., Pb2+, Hg2+, Cr6+, Cd2+, or Cu2+) greater than their permissible limits adversely affects human health, and increases the risk of cancer of the kidneys, liver, skin, and central nervous system. Therefore, their detection in water is crucial. Due to the various benefits of "green"-synthesized carbon-dots (C-dots) over other materials, these materials are potential candidates for sensing of toxic heavy-metal ions in water sources. C-dots are very small carbon-based nanomaterials that show chemical stability, magnificent biocompatibility, excitation wavelength-dependent photoluminescence (PL), water solubility, simple preparation strategies, photoinduced electron transfer, and the opportunity for functionalization. A new family of C-dots called "carbon quantum dots" (CQDs) are fluorescent zero-dimensional carbon nanoparticles of size < 10 nm. The green synthesis of C-dots has numerous advantages over conventional chemical routes, such as utilization of inexpensive and non-poisonous materials, straightforward operations, rapid reactions, and renewable precursors. Natural sources, such as biomass and biomass wastes, are broadly accepted as green precursors for fabricating C-dots because these sources are economical, ecological, and readily/extensively accessible. Two main methods are available for C-dots production: top-down and bottom-up. Herein, this review article discusses the recent advancements in the green fabrication of C-dots: photostability; surface structure and functionalization; potential applications for the sensing of hazardous anions and toxic heavy-metal ions; binding of toxic ions with C-dots; probable mechanistic routes of PL-based sensing of toxic heavy-metal ions. The green production of C-dots and their promising applications in the sensing of hazardous ions discussed herein provides deep insights into the safety of human health and the environment. Nonetheless, this review article provides a resource for the conversion of low-value biomass and biomass waste into valuable materials (i.e., C-dots) for promising sensing applications.

Characterization and cytotoxicity assessment of cadmium sulfide quantum dots synthesized with Fusarium oxysporum f. sp. lycopersici.

The potential of CdS quantum dots for biomedical and bioimaging applications depends on their cytotoxicity, which can be modulated by coating molecules. Using sulfur as a precursor can be used along with cadmium nitrate to synthesize CdS quantum dots with the fungus Fusarium oxysporum f. sp. lycopersici. The latter replaces pure chemical sulfur as a precursor for CdS quantum dot synthesis, thus transforming waste into a value-added product, increasing sustainability, reducing the environmental impact of the process through the implementation of green synthesis techniques, and contributing to the circular economy. Therefore, we compared the cytotoxicity on HT-29 cells of biogenic, and chemical CdSQDs, synthesized by a chemical method using pure sulfur. Biogenic and chemical CdSQDs had diameters of 4.08 ± 0.07 nm and 3.2 ± 0.20 nm, Cd/S molar ratio of 43.1 and 1.1, Z-potential of - 14.77 ± 0.64 mV and - 5.52 ± 1.11 mV, and hydrodynamic diameters of 193.94 ± 3.71 nm and 152.23 ± 2.31 nm, respectively. The cell viability improved 1.61 times for biogenic CdSQDs over chemical CdSQDs, while cytotoxicity, measured as IC50, diminished 1.88-times. The lower cytotoxicity of biogenic CdSQDs was attributed to their organic coating consisting of lipids, amino acids, proteins, and nitrate groups that interacted with CdS through -OH and -SH groups. Therefore, the biogenic synthesis of CdSQDs has repurposed a pathogenic fungus, taking advantage of the biomolecules it secretes, to transform hazardous sulfur waste and metal ions into stable CdSQDs with advantageous structural and cytotoxic properties for their potential application in biomedicine and bioimaging.

An update on the biological effects of quantum dots: From environmental fate to risk assessment based on multiple biological models.

Quantum dots (QDs) are zero-dimension nanomaterials with excellent physical and chemical properties, which have been widely used in environmental science and biomedicine. Therefore, QDs are potential to cause toxicity to the environment and enter organisms through migration and bioenrichment effects. This review aims to provide a comprehensive and systematic analysis on the adverse effects of QDs in different organisms based on recently available data. Following PRISMA guidelines, this study searched PubMed database according to the pre-set keywords, and included 206 studies according to the inclusion and elimination criteria. CiteSpace software was firstly used to analyze the keywords of included literatures, search for breaking points of former studies, and summarize the classification, characterization and dosage of QDs. The environment fate of QDs in the ecosystems were then analyzed, followed with comprehensively summarized toxicity outcomes at individual, system, cell, subcellular and molecular levels. After migration and degradation in the environment, aquatic plants, bacteria, fungi as well as invertebrates and vertebrates have been found to be suffered from toxic effects caused by QDs. Aside from systemic effects, toxicity of intrinsic QDs targeting to specific organs, including respiratory system, cardiovascular system, hepatorenal system, nervous system and immune system were confirmed in multiple animal models. Moreover, QDs could be taken up by cells and disturb the organelles, which resulted in cellular inflammation and cell death, including autophagy, apoptosis, necrosis, pyroptosis and ferroptosis. Recently, several innovative technologies, like organoids have been applied in the risk assessment of QDs to promote the surgical interventions of preventing QDs' toxicity. This review not only aimed at updating the research progress on the biological effects of QDs from environmental fate to risk assessment, but also overcame the limitations of available reviews on basic toxicity of nanomaterials by interdisciplinarity and provided new insights for better applications of QDs.

Effective photodegradation of rhodamine B and levofloxacin over CQDs modified BiOCl and BiOBr composite: Mechanism and toxicity assessment.

In this contribution, carbon quantum dots (CQDs) modified 3D-flower like BiOX (X = Cl, Br, I) photocatalyst were successfully prepared via a facile mechanical compounding method. The crystal structure, surface composition, morphologies, optical properties and photocatalytic activities were investigated in detail. The photocatalytic activity of the as-obtained photocatalyst were evaluated by degradation of rhodamine B (RhB) and Levofloxacin (LEV) under near IR-UV-vis light irradiation, the CQDs/BiOX composite displayed enhanced photocatalytic activity as compared with individual BiOX materials. The CQDs/BiOX composite had the outstanding light harvesting and electron transfer ability because of the ordered ultrathin nanosheet structure of the BiOX, the formation of metal Bi under photoinduction, and the synergistic effects between CQDs and pure BiOX. Antibacterial activity and effects on Rye seeds growth of the LEV degradation intermediate were also researched. Reactive-species-trapping experiments exhibited that h+ and O2- were the active reactive species during photodegradation process. This work provided an effective and simple strategy for designing QDs modified Bi-rich oxyhalides in organic pollutant containing wastewater treatment.

Systematic toxicity assessment of CdTe quantum dots in Drosophila melanogaster.

The risk assessment of cadmium (Cd)-based quantum dots (QDs) used for biomedical nanotechnology applications has stern toxicity concerns. Despite cytotoxicity studies of cadmium telluride (CdTe) QDs, the systematic in vivo study focusing on its organismal effects are more relevant to public health. Therefore, the present study aims to investigate the effect of chemically synthesized 3-mercapto propionic acid-functionalized CdTe QDs on organisms' survival, development, reproduction, and behaviour using Drosophila melanogaster as a model. The sub-cellular impact on the larval gut was also evaluated. First/third instar larvae or the adult Drosophila were exposed orally to green fluorescence emitting CdTe QDs (0.2-100 µM), and organisms' longevity, emergence, reproductive performance, locomotion, and reactive oxygen species (ROS), and cell death were assessed. Uptake of semiconductor CdTe QDs was observed as green fluorescence in the gut. A significant decline in percentage survivability up to 80% was evident at high CdTe QDs concentrations (25 and 100 µM). The developmental toxicity was marked by delayed and reduced fly emergence after CdTe exposure. The teratogenic effect was evident with significant wing deformities at 25 and 100 µM concentrations. However, at the reproductive level, adult flies' fecundity, fertility, and hatchability were highly affected even at low concentrations (1 µM). Surprisingly, the climbing ability of Drosophila was unaffected at any of the used CdTe QDs concentrations. In addition to organismal toxicity, the ROS level and cell death were elevated in gut cells, confirming the sub-cellular toxicity of CdTe QDs. Furthermore, we observed a significant rescue in CdTe QDs-associated developmental, reproductive, and survival adversities when organisms were co-exposed with N-acetyl-cysteine (NAC, an antioxidant) and CdTe QDs. Overall, our findings indicate that the environmental release of aqueously dispersible CdTe QDs raises a long-lasting health concern on the development, reproduction, and survivability of an organism.

Quantum dot clusters as self-assembled antennae with phycocyanine and phycobilisomes as energy acceptors.

In this study, we investigated an experimental and Monte-Carlo computational characterization of self-assembled antennae built using CdTe colloidal quantum dots (QDs). These clusters provide efficient excitation of phycocyanine (PC) or phycobilisomes (PBSs). PBSs are light-harvesting complexes (LHCs) of cyanobacteria, made of several PC units, organized in disks and rods. Each PC contains three separate cofactors. Therefore, we analyzed variations in multi-donor and multi-acceptor systems. The self-assembled QD clusters were formed mostly by electrostatic interactions, possibly due to the introduction of a positive charge on an originally negatively charged nanoparticle surface. Our results suggest that PC may accept energy from multiple nanoparticles localized at a distance significantly longer than the Förster radius. The excitation transfers between particular nanoparticles with possible delocalization. The maximal energy transfer efficiency was obtained for the PC/PBS : QD ratio from 1 to 20 depending on the QD size. This cannot be fully explained using computational simulations; hence, we discussed the hypothesis and explained the observations. Our self-assembled systems may be considered for possible applications in artificial light-harvesting systems because absorption spectra of QDs are different from the absorption characteristics of PC/PBS. In addition, huge clusters of QDs may effectively increase the optical cross-section of so-created nanohybrids.

In vitro toxicity assessment of fungal-synthesized cadmium sulfide quantum dots using bacteria and seed germination models.

There is currently controversy over the use of quantum dots (QDs) in biological applications due to their toxic effects. Therefore, the purpose of the present study was to evaluate the toxic effect of chemical and biogenic (synthesized by Fusarium oxysporum f. sp. lycopersici) cadmium sulfide quantum dots (CdSQDs) using a bacterial model of Escherichia coli and sprouts of Lactuca sativa L. with the aim to foresee its use in the near future in biological systems. Physicochemical properties of both types of CdSQDs were determined by TEM, XRD, zeta potential and fluorescence spectroscopy. Both biogenic and chemical CdSQDs showed agglomerates of spherical CdSQDs with diameters of 4.14 nm and 3.2 nm, respectively. The fluorescence analysis showed a band around 361 nm in both CdSQDs, the zeta potential was -1.81 mV for the biogenic CdSQDs and -5.85 mv for the chemical CdSQDs. Results showed that chemical CdSQDs, presented inhibition in the proliferation of E. coli cell in a dose-dependent manner, unlike biogenic CdSQDs, that only at its highest concentration showed an antibacterial activity. Also, it was observed that after incubation with chemical and biogenic CdSQDs of L. sativa L. seeds, only the biogenic CdSQDs showed no inhibition on seed germination. In summary, our results suggest that the production route has a significant effect on the toxicity of QDs; in addition, it seems that the biological coating of the CdSQDs from F. oxysporum f. sp. lycopersici inhibit their toxic effect on bacterial strains and plant seeds.

Rapid and highly sensitive visual detection of oxalate for metabolic assessment of urolithiasis via selective recognition reaction of CdTe quantum dots.

Urolithiasis is a common disease that affects 5% to 8.8% of the world population with high recurring frequency. Therefore, there is an urgent need for the rapid and efficient diagnostics of urolithiasis. In this study, we developed a quantum dots (QDs)-based sensor for detecting urolithiasis oxalate. Urolithiasis oxalate was quantified by reducing Cu2+ to Cu+, which can be selectively recognized by CdTe QDs. The homogeneous sensing system shows high sensitivity for oxalate detection in the range of 0.1 µM to 10 mM within 6 min; moreover, the visual detection of 10 µM oxalate could be achieved by the naked eye. Our method was tested on 53 clinical samples; it showed 100% specificity and sensitivity for calcium oxalate urolithiasis compared with clinical diagnosis, even for hematuria samples. Furthermore, this method can be expanded to other wide range of biochemistry applications for medical diagnosis and point-of-care testing.

Phase Transfer and DNA Functionalization of Quantum Dots Using an Easy-to-Prepare, Low-Cost Zwitterionic Polymer.

Small, stable, and bright quantum dots (QDs) are of interest in many biosensing and biomedical imaging applications, but current methodologies for obtaining these characteristics can be highly specialized or expensive. We describe a straightforward, low-cost protocol for functionalizing poly(isobutylene-alt-maleic anhydride) (PIMA) with moieties that anchor to the QD surface (histamine), impart hydrophilicity [(2-aminoethyl)trimethylammonium chloride (Me3N+-NH2)], and provide a platform for biofunctionalization via click chemistry (dibenzocyclooctyne (DBCO)). Guidelines to successfully use this polymer for QD ligand exchange are presented, and an example of biofunctionalization with DNA is shown. Stable QD-DNA conjugates are obtained with high yield and without requiring additional purification steps.

Quantum Dots for Assessment of Reactive Oxygen Species Accumulation During Chemotherapy and Radiotherapy.

Quantum dots (QDs) are semiconductor nanoparticles ranging in size from 2 to 10 nm. QDs are increasingly being developed for biomedical imaging, targeted drug delivery, and green energy technology. Here we describe the novel utilization of biocompatible CdSe-ZnS core-shell semiconductor nanoparticles for assessment of reactive oxygen species (ROS) in the context of chemotherapy and radiotherapy, both of which are important modalities in the treatment of cancer.

Biosynthetic Mycotoxin Conjugate Mimetics-Mediated Green Strategy for Multiplex Mycotoxin Immunochromatographic Assay.

Various mycotoxins widely co-exist in agro-products, and their combined effects cause toxicity and potential carcinogenicity to humans and animals. In this work, we developed an economical and sensitive quantum dots (QDs)/QD microbead (QDs/QB)-based multiplex immunochromatographic assay (mICA) for the rapid detection of fumonisin B1 (FB1), zearalenone (ZEN), and ochratoxin A (OTA) without the building-up process of mycotoxin conjugates. QDs and QBs were selected as fluorescent reporters and conjugated with antimycotoxin monoclonal antibodies for improving sensitivity. Furthermore, phage-displayed FB1, ZEN, and OTA mimotope peptide-based soluble and monovalent fusions to maltose-binding protein (MBP) were applied onto the test line of the mICA as the mimetic coating antigen. Under the optimized conditions, the visual detection limits (vLODs) of peptide-MBP-based mICA could be obtained as 0.25 ng/mL for FB1, 3.0 ng/mL for ZEN, and 0.5 ng/mL for OTA within 10 min. The results for spiked real sample detection indicate good accuracy, reproducibility, and practicability. In addition, the proposed mICA was comparable with ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) in terms of reliability in detecting FB1, ZEN, and OTA using natural samples. From the point of promoting commercial production, these time-saving and low-cost peptide-MBP antigens applied in ICA might provide promising potential for promoting productivity and decreasing the cost of production.

A new electrochemical platform based on low cost nanomaterials for sensitive detection of the amoxicillin antibiotic in different matrices.

A new electrochemical device based on a combination of nanomaterials such as Printex 6L Carbon and cadmium telluride quantum dots within a poly(3,4-ethylenedioxythiophene) polystyrene sulfonate film was developed for sensitive determination of amoxicillin. The morphological, structural and electrochemical characteristics of the nanostructured material were evaluated using X-ray diffraction, confocal microscopy, transmission electron microscopy and voltammetric techniques. The synergy between these materials increased the electrochemical activity, the electron transfer rate and the electrode surface area, leading to a high magnitude of the anodic peak current for the determination of amoxicillin. The electrochemical determination of the antibiotic was carried out using square-wave voltammetry. Under the optimised experimental conditions, the proposed sensor showed high sensitivity, repeatability and stability to amoxicillin determination, with an analytical curve in the amoxicillin concentration range from 0.90 to 69 µmol L-1, and a low detection limit of 50 nmol L-1. No significant interference in the electrochemical signal of amoxicillin was observed from potential biological interferences and drugs widely used, such as uric acid, paracetamol, urea, ascorbic acid and caffeine. It was demonstrated that without any sample pre-treatment and using a simple measurement device, the sensor could be an alternative method for not only the analysis of pharmaceutical products (commercial tablets) and clinical samples (urine), but also to examine food quality (milk samples).

Facile, large scale synthesis of water soluble AgInSe2/ZnSe quantum dots and its cell viability assessment on different cell lines.

I-III-VI chalcopyrite ternary quantum dots have emerged as a good alternative over the conventional II-VI and IV-VI chalcogenide binary QDs that usually consist of heavy metals such as Cd and Pb which has limited their bioapplications. Among the chalcopyrite QDs, AgInSe2 QDs has been the least developed due to the imbalanced cation reactivity, unwanted impurities, broad size distribution and resultant large particle sizes. In addition, the cell viability of these QDs still needs to be investigated on different cell lines both normal and cancerous ones. Herein, large-scale synthesis of water-soluble thioglycolic acid (TGA) capped and gelatin-stabilized AgInSe2 (AISe) core and AgInSe2/ZnSe (AISe/ZnSe) core/shell QDs in the absence of an inert atmosphere and their cell viability against different cell lines are reported. The optical and structural characteristics of the as-synthesized QDs were investigated by UV-visible (vis) absorption, photoluminescence (PL) and Fourier-transmission infrared (FTIR) spectroscopies, dynamic light scattering (DLS), X-ray diffraction (XRD), and high-resolution transmission electron microscope (HRTEM) techniques. Growth of ZnSe shell on the core AISe resulted in the blue shifting of the emission maximum position with the increased PL intensity. The QDs are small and spherical in shape with an average particle diameter of 2.8 nm and 3.2 nm for AISe and AISe/ZnSe QDs respectively. The in vitro cell viability assay revealed that the as-synthesized AISe/ZnSe QDs are not toxic towards cancerous (HeLa -cervical cancer and A549-lung cancer) and normal (BHK21 -Kidney) cell lines.

Ammonium Fluoride Passivation of CdZnTeSe Sensors for Applications in Nuclear Detection and Medical Imaging.

Cadmium zinc telluride selenide (Cd1-xZnxTe1-ySey or CZTS) is one of the emerging CdTe-based semiconductor materials for detecting X- and gamma-ray radiation at or near room temperature (i.e., without cryogenic cooling). Potential applications of CZTS sensors include medical imaging, X-ray detection, and gamma-ray spectroscopy. Chemical passivation of CZTS is needed to reduce the conductivity of Te-rich surfaces, which reduces the noise and improves the device performance. In this study, we focus on the effect of surface passivation of CZTS using a 10% aqueous solution of ammonium fluoride. The effects of the chemical treatment were studied on the leakage current, charge transport measured as the electron mobility-lifetime (µτ) product, and the spectral resolution measured as the full-width at half-maximum (FWHM) of specific peaks. After passivation, the leakage current increased and began to decrease towards pre-passivation levels. The energy resolutions were recorded for eight applied voltages between -35 V and -200 V. The results showed an average of 25% improvement in the detector's energy resolution for the 59.6 keV gamma peak of Am-241. The electron µτ product was unchanged at 2 × 10-3 cm2/V. These results show that ammonium fluoride is effective for chemical passivation of CZTS detectors.

Microwave Assisted Synthesis of N-Doped Carbon Dots: an Easy, Fast and Cheap Sensor for Determination of Aspartic Acid in Sport Supplements.

In this work, determination of aspartic acid by N-doped carbon dots (N-CDs) was studied at optimum condition. Characterization and morphology of surface of N-CDs were carried out by FT-IR and HRTEM. N-doped carbon dots size was 10 nm. Quenching was very fast after addition of aspartic acid that is an important property of this sensor. Optimum conditions for pH and excitation wavelength were 8 and 360 nm, respectively. Linear dynamic range and limit of detection for aspartic acid were 0.5-50 µM and 90 nM, respectively. This method was used for aspartic acid determination in human serum and sport supplement powder as real samples. Performance of this sensor was also compared with other fluorescent sensors.

Materials for Photovoltaics: State of Art and Recent Developments.

In recent years, photovoltaic cell technology has grown extraordinarily as a sustainable source of energy, as a consequence of the increasing concern over the impact of fossil fuel-based energy on global warming and climate change. The different photovoltaic cells developed up to date can be classified into four main categories called generations (GEN), and the current market is mainly covered by the first two GEN. The 1GEN (mono or polycrystalline silicon cells and gallium arsenide) comprises well-known medium/low cost technologies that lead to moderate yields. The 2GEN (thin-film technologies) includes devices that have lower efficiency albeit are cheaper to manufacture. The 3GEN presents the use of novel materials, as well as a great variability of designs, and comprises expensive but very efficient cells. The 4GEN, also known as "inorganics-in-organics", combines the low cost/flexibility of polymer thin films with the stability of novel inorganic nanostructures (i.e., metal nanoparticles and metal oxides) with organic-based nanomaterials (i.e., carbon nanotubes, graphene and its derivatives), and are currently under investigation. The main goal of this review is to show the current state of art on photovoltaic cell technology in terms of the materials used for the manufacture, efficiency and production costs. A comprehensive comparative analysis of the four generations is performed, including the device architectures, their advantages and limitations. Special emphasis is placed on the 4GEN, where the diverse roles of the organic and nano-components are discussed. Finally, conclusions and future perspectives are summarized.