RESUMO
Porcine deltacoronavirus (PDCoV) is an emerging swine enteric coronavirus that causes diarrhea in piglets. However, the biological characteristics of PDCoV are unclear. In this study, the hemagglutination (HA) abilities of two PDCoV strains (CH-01 and HNZK-04) were investigated. Our results showed that PDCoV has the ability to agglutinate rabbit erythrocytes after virion pretreatment with trypsin or neuraminidase. Additionally, the HA assay results showed a significant positive correlation with the infectious viral titer. Our results suggest that assessing the HA activity of PDCoV may be a useful diagnostic method for investigating and surveilling PDCoV infections.
Assuntos
Infecções por Coronavirus/veterinária , Coronavirus/fisiologia , Hemaglutinação , Doenças dos Suínos/imunologia , Animais , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/virologia , Diarreia/imunologia , Diarreia/veterinária , Diarreia/virologia , Eritrócitos/imunologia , Neuraminidase/administração & dosagem , Coelhos , Suínos , Doenças dos Suínos/virologia , Tripsina/administração & dosagem , Vírion/efeitos dos fármacosRESUMO
The present investigation has demonstrated that when cell layers are trypsinized with 125I-trypsin and washed under strictly standardized conditions, the amount of trypsin which remains associated with the cell suspension can be accurately determined. This amount is more than 10 times greater than would be expected from dilution only and is dependent on the density of the cell layers. On plating of the cells, less than 10% of the carry-over trypsin remains associated with the cells and suggestive evidence for a mainly intracellular localization was obtained with a quantitative immunoperoxidase technique. This carry-over trypsin was further shown to maintain proteolytic activity by its ability to remove significant amounts of macromolecular material from a cell layer prelabeled with 3H-Leucine.