Optimization of a QuEChERS-LC-MS/MS method for 51 pesticide residues followed by determination of the residue levels and dietary intake risk assessment in foodstuffs.

A modified QuEChERS purification-liquid chromatography-tandem mass spectrometry method has been developed to determine 51 pesticide residues with newly established maximum residue limits (MRLs) in foodstuffs. Samples were extracted with acetonitrile under citrate-buffered conditions and purified using a modified QuEChERS method employing hydroxylated MWCNTs, SAX, and C18. The limits of quantification ranged from 0.2 to 9.8 µg/kg. Recoveries in ten different foodstuffs ranged from 70.2% to 117.9%, with relative standard deviations between 2.3% and 19.9% at three spiking levels. This method was applied to analyze 352 market samples, detecting 14 pesticides in 97 samples. Notably, Afidopyropen, cyantraniliprole, and fluxapyroxad residues in vegetables exhibited a consistent pattern of higher levels in the spring and winter and lower levels in the summer and autumn. Moreover, the risk assessments for acute and chronic dietary exposure to the 14 detected pesticides indicated that the %ADI and %ARfD were well below 100%.

Assessment of asphaltene and resin fractions in crude oil using laser-induced fluorescence spectroscopy based on modified Beer-Lambert (LIFS-MBL).

Crude oil is one of the most significant petrogenic sources of polycyclic aromatic compounds (PACs). These substances play an essential role in the pollution of the marine environment. Therefore, the rapid identification of this pollutant source and its fractions is vital. For this purpose, a fast and on-site method of laser-induced fluorescence spectroscopy based on modified Beer-Lambert (LIFS-MBL) is proposed here using solvent densitometry. Three optical parameters of the self-quenching (K), the extinction (α), and the peak concentration (Cp) are experimentally extracted from MBL graphs. Note that the parameters above are known to be unique characteristics of various crude oils. The corresponding compounds are generally classified into saturate, aromatic, resin, and asphaltene fractions, abbreviated as SARA. Differentiation among these fractions is achieved using the LIFS-MBL method by selecting the optimal excitation wavelength at 405 nm. This line effectively rules out the light aromatic rings and focuses on heavy fractions. The correlation of optical parameters with heavy oil fractions is verified according to analysis of variance. Statistical relations are proposed to calculate crude oil fractions values. The values of light fractions including saturate and aromatic components can also be determined by the heavy fractions. In this method, the test time is notably reduced from four days using the standard methods to less than half an hour according to the presented LIFS-MBL technique.

Primary assessment of macronutrients in durian (CV Monthong) leaves using near infrared spectroscopy with wavelength selection.

Farmers would be able to regulate fertilization and produce quality durian if they knew the nutrient concentration in durian leaves. A long period of time for traditional nutritional content determination is needed. Therefore, near-infrared spectroscopy is a good method for nondestructive and quick nutrient content evaluation. The leaf sample matrices (fresh leaves, dried ground leaves, and dried ground leaf pellets) were scanned by Fourier transform near-infrared (FT-NIR) with a wavelength of 12,500-3,600 cm-1. Regression models were developed using partial least squares (PLS) with full wavelength, short wavelength, and selected wavelength by successive projections algorithm (SPA). In this study, the model for N and K concentration was acceptable and the prediction was considered good but for P content not had succeeded. As a result, the PLS-SPA model using fresh leaf samples for evaluating N content in durian leaves exhibited performance of r2 = 0.852, SEP = 0.14%, RPD = 2.63 and bias = -0.020%. The PLS-SPA model using dried ground leaf samples for evaluating K content in durian leaves exhibited performance of r2 = 0.820, SEP = 0.13%, RPD = 2.36 and bias = 0.006%. This research found that it is possible to apply NIR waves to predict N and K concentrations in durian leaves. It is not necessary to predict directly from the wavelengths associated with -N or -K bonds. Instead, NIR can measure them indirectly from the bonding of proteins, which are products formed by N and K. In addition, selecting the wavelength that is related to the value to be measured can produce results that are not significantly different from using full or short wavelengths. These models can assist farmers in rapidly predicting N and K content in durian leaves for immediate fertilizer adjustment.

DeepTrayMeal: Automatic dietary assessment for Chinese tray meals based on deep learning.

Tray meal is a popular way of eating in China, and tray-based automatic dietary assessment is important for public health. Relevant research is lacking because public tray meal datasets and suitable methods are unavailable. In this study, we established and published the first Chinese tray meal dataset, the ChinaLunchTray-99. We collected real-world 1185 tray meal images, covering 99 dish categories with corresponding manually annotated bounding box and category-level labels. We developed a new framework for automatic dietary assessment, which consists of dish image recognition, volume estimation and nutrition mapping. First, we demonstrated a tray meal detection model considering feature extraction, anchor scales, and loss function, resulting in a high mean Average Precision of 92.13%. Second, we proposed an automatic method to estimate volume via detection results and tray's information. Finally, nutrients were mapped from the estimated volume. Our research can promote applications of automatic dietary assessment for Chinese tray meals.

The role of the Arctic Monitoring and Assessment Programme (AMAP) in reducing pollution of the Arctic and around the globe.

This article presents the initiation and implementation of a systematic scientific and political cooperation in the Arctic related to environmental pollution and climate change, with a special focus on the role of the Arctic Monitoring and Assessment Programme (AMAP). The AMAP initiative has coordinated monitoring and assessments of environmental pollution across countries and parameters for the entire Arctic region. Starting from a first scientific assessment in 1998, AMAP's work has been fundamental in recognizing, understanding and addressing environmental and human health issues in the Arctic, including those of persistent organic pollutants (POPs), mercury, radioactivity, oil, acidification and climate change. These scientific results have contributed at local and international levels to define and take measures towards reducing the pollution not only in the Arctic, but of the whole globe, especially the contaminant exposure of indigenous and local communities with a traditional lifestyle. The results related to climate change have documented the rapid changes in the Arctic and the strong feedback between the Arctic and the rest of the world. The lessons learned from the work in the Arctic can be beneficial for other regions where contaminants may accumulate and affect local and indigenous peoples living in a traditional way, e.g. in the Himalayas. Global cooperation is indispensable in reducing the long-range transported pollution in the Arctic.

Assessment of Endothelial Barrier Functions in Extra Embryonic Vasculature of Chick Embryo as an Alternative Model.

Vascular permeability, a tightly regulated process, is a direct measure of angiogenic and immune responses in the endothelium altered in several acute and chronic diseases such as sepsis, high-altitude pulmonary edema (HAPE), high-altitude cerebral edema (HACE), ischemia, and coronavirus disease 2019 (COVID-19) endotheliitis. Both endogenous and exogenous factors such as cytokines, chemokines, and hormones may affect vascular permeability. The conventional tools available for the measurement of vascular permeability in vitro and in vivo based on collagen-coated Transwell and dye-based spectrophotometric methods are indirect measures of permeability. In this chapter, we present our live in ovo protocols based on dextran-Texas red and avian chorioallantoic membrane assay developed using custom-made equipment to assess leakiness of endothelial cell barrier both in vitro and in vivo. Further, we validate this assay using different stressors such as ischemia and hypoxia known to affect endothelial barrier properties by potentiating actin stress fiber disorganization and disrupting the cell-cell junctions.

Reliability of an AI-Powered Application Across Different Mobile Devices for Assessment of Chronic Wounds.

Objective: Evaluate the inter- and intrarater reliability of a wound assessment tool in iPhone 12 and 13 mini modalities against a validated iPad mini/Structure Sensor configuration. Approach: We assessed a wound measurement application (eKare inSight®) for result consistency in patients presenting with wounds. Assessments were analyzed using a two-way analysis of variance. Intraclass correlation coefficient (ICC) was computed for intrarater (ICC1,1) and inter-rater (ICC2,1) analysis using a two-way random effects model. Paired t-test assessed the statistical difference between measurement methods. Results: Forty-two lesions were analyzed with surface areas ranging from 0.2 to 23 cm2 (average 4.33 ± 5.44 cm2). A high level of reliability was observed for repeat wound area measurements by the same examiner (ICC1,1 = 0.997) and between examiners with iPhone 13 mini (ICC2,1 = 0.998). There was no significant difference between iPhone 12 and iPad mini/Structure Sensor (p = 0.78) or between iPhone 13 mini and iPhone 12 (p = 0.22). Minimal difference existed between iPhone 13 mini and iPad mini/Structure Sensor (p = 0.049, Cohen's d = 0.01). Innovation: Increased pervasiveness of smartphones in clinical care, coupled with advances in smartphone imaging and machine learning, allows for a potential solution to the problem of fast and accurate wound measurements. The application investigated produces wound measurement results quickly and with demonstrated accuracy. It does not require a calibration sticker or reference marker and allows for automatic wound boundary delineation. Conclusion: The results of this study suggest that a digital planimetry mobile application may offer high levels of reliability across devices and users.

Sustainable approach towards isolation of photosynthetic pigments from Spirulina and the assessment of their prooxidant and antioxidant properties.

Carotenoids, chlorophyll and phycocyanin are three types of photosynthetic pigments found in Spirulina that differ in colour, composition, stability, solubility, and commercial importance. Such diversity of structures creates a challenge to extract these pigments simultaneously from the same batch of raw material in an efficient and sustainable manner. This study demonstrates that water can be successfully used as a single solvent together with combined (non)mechanical cell membrane disruption techniques (ultrasonication, centrifugation, freezing/thawing cycle) to extract these photosynthetic pigments from the same batch. This water-based approach delivers a significant improvement in isolating green pigments, which are often overlooked during extraction due to a preference for blue and yellow pigments. Chlorophyll was quantitatively converted to its stable derivatives to carry out a comparative analysis of antioxidant properties (DPPH, TEAC, FRAP), singlet oxygen production and intracellular activities (MTT, ROS assays) using Caco-2 cells.

Qualitative and quantitative assessment of key aroma compounds, advanced glycation end products and heterocyclic amines in different varieties of commercially roasted meat products.

Studies on the interactions and links between aroma and hazardous compounds were inadequately investigated. A complete analysis was conducted on the key aroma compounds, typical hazardous compounds and their precursors in 25 samples of roasted meats. Forty-nine aroma compounds were identified as essential odorants with odor-activity values exceeds 1. Nε-carboxymethyl lysine (CML, 11.78-49.32 µg/g) and Nε-carboxyethyl lysine (CEL, 8.48-171.00 µg/g) were identified as representative advanced glycation end products (AGEs) of meats with high concentrations. Harman and Norharman were typical heterocyclic aromatic amines. Meanwhile, correlation analysis indicated that aldehyde and alcohols showed a negative correlation with AGEs (p < 0.01), while pyrazines might affect the formation of Harman and Norharman. The furaldehyde, 1-hexanol, 2, 4-Decadienal, AGEs, and creatine were regarded as potential biomarkers that distinguished different roasted meat products. Therefore, the study could provide new insights for synergistic regulation of aroma and hazardous compounds in roasted meat products.

Epidemiological and clinical features, treatment status, and economic burden of traumatic spinal cord injury in China: a hospital-based retrospective study.

Traumatic spinal cord injury is potentially catastrophic and can lead to permanent disability or even death. China has the largest population of patients with traumatic spinal cord injury. Previous studies of traumatic spinal cord injury in China have mostly been regional in scope; national-level studies have been rare. To the best of our knowledge, no national-level study of treatment status and economic burden has been performed. This retrospective study aimed to examine the epidemiological and clinical features, treatment status, and economic burden of traumatic spinal cord injury in China at the national level. We included 13,465 traumatic spinal cord injury patients who were injured between January 2013 and December 2018 and treated in 30 hospitals in 11 provinces/municipalities representing all geographical divisions of China. Patient epidemiological and clinical features, treatment status, and total and daily costs were recorded. Trends in the percentage of traumatic spinal cord injuries among all hospitalized patients and among patients hospitalized in the orthopedic department and cost of care were assessed by annual percentage change using the Joinpoint Regression Program. The percentage of traumatic spinal cord injuries among all hospitalized patients and among patients hospitalized in the orthopedic department did not significantly change overall (annual percentage change, -0.5% and 2.1%, respectively). A total of 10,053 (74.7%) patients underwent surgery. Only 2.8% of patients who underwent surgery did so within 24 hours of injury. A total of 2005 (14.9%) patients were treated with high-dose (≥ 500 mg) methylprednisolone sodium succinate/methylprednisolone (MPSS/MP); 615 (4.6%) received it within 8 hours. The total cost for acute traumatic spinal cord injury decreased over the study period (-4.7%), while daily cost did not significantly change (1.0% increase). Our findings indicate that public health initiatives should aim at improving hospitals' ability to complete early surgery within 24 hours, which is associated with improved sensorimotor recovery, increasing the awareness rate of clinical guidelines related to high-dose MPSS/MP to reduce the use of the treatment with insufficient evidence.

Solar photocatalysis application in UWWTP outlets - simulations based on predictive models in flat-plate reactors and pollutant degradation studies with in silico toxicity assessment.

The application of the solar photocatalysis for the degradation of residual pollutants found in surface water was demonstrated. Semi-pilot scale flat-plate cascade reactor (FPCR) was used to study the degradation of model organic pollutants: enrofloxacin (ENRO), 17ß-estradiol (E2) and 1H-benzotriazole (1H-BT) over TiO2 thin-film supported on glass fibers. A modular panel with full-spectra solar lamps with appropriate UVB and UVA irradiation levels was used as a simulation of sunlight. Pollutant degradation in FPCR was estimated using predictive models; intrinsic reaction rate constants (ki) for ENRO, E2 and 1H-BT independent of the reactor size, flow rate and irradiation conditions were determined: 9.60, 3.35 and 0.37 109 s-1 W-0.5 m1.5, respectively. Main degradation products (DPs), formed upon hydroxylation, ring opening and oxidation, were identified using LC-QTOF-MS. The ecotoxicological impact was assessed via T.E.S.T. and ECOSAR open-source tools showing the formation of less harmful DPs after sufficient reaction time. Pollutant degradation was simulated at four locations of interest, i.e. exhausts from urban wastewater treatment plants (UWWTPs) in Zagreb, Croatia (45°N), Krakow, Poland (50°N), Sevilla, Spain (37°N) and Ioannina, Greece (39.6°N). Results have proved that a simple flat-plate system with supported photocatalysts can be easily scaled up and incorporated at the outlet of UWWTP for the reduction of pollutant load and related toxicity. The exhaust canal in Zagreb with the estimated length of a photocatalytic layer of 122 m for the > 90% degradation of all target pollutants was discussed as the best installation site among studied locations. ENVIRONMENTAL IMPLICATION: A multi-disciplinary approach to the tentative application of TiO2 solar photocatalysis outdoors to reduce pollutant loads and toxicity in surface waters was demonstrated. Possible application at four selected locations in Europe, as an additional step in water treatment after urban wastewater treatment plants (UWWTPs) was discussed. Target pollutants were studied under environmentally relevant conditions (sunlight levels, water matrix, simulation of process on a real scale at selected geographical location), at both higher and low concentrations.

Bisphenol A contamination in Hilsa shad and assessment of potential health hazard: A pioneering investigation in the national river Ganga, India.

The anadromous Hilsa, often known as the "Queen of Fishes" (Tenualosa ilisha), is the most valuable fishery in the Ganga-Hooghly delta estuary. Although BPA exposure has been shown to be harmful to aquatic organisms, no research has looked at the effects of BPA on the commercially valuable Hilsa shad of river Ganga. To close this information vacuum, we examined BPA levels in Hilsa fish from the Ganga estuary. Liver, muscle, kidney, and gonads were all positive for BPA among the Hilsa fish of all ages. Liver BPA levels were highest in adult males (272.16 ± 0.38 ng/g-dw), and lowest in juveniles (5.46 ± 0.06 ng/g-dw). BPA concentrations in the Hilsa shad muscle were highest in reproductively mature females (196.23 ± 0.41 ng/g-dw). The study also discovered a correlation between fish development and BPA exposure, with higher levels of BPA being identified in adult Hilsa species. This is the first study to look at the impact of BPA pollution on aquatic ecosystems and fisheries, and it showed that Hilsa shad is contaminated with BPA and poses health hazards to human beings. The results, which demonstrate BPA contamination, are useful for protecting Hilsa in the river Ganga.

Microplastic pollution and ecological risk assessment of Yueqing Bay affected by intensive human activities.

Microplastics (MPs) are a widespread environmental problem posing ecological risks in the ocean. We investigated the abundance, spatial distribution, characteristics and ecological risks of MPs in surface seawater, sediments and organisms in Yueqing Bay, China. MPs were detected in both environmental media and organisms. The overall abundance (0.24 items/m3 seawater, 6.13 items/kg dry sediment, 0.77 items/individual in organisms) was low to medium compared with other coastal areas. The MPs were mainly derived from the high-intensity mariculture and shipping in the bay, as well as industrial and human activities along the surrounding coast. The abundance of MPs in water of the left (western) bay (0.39 items/m3) was considerably higher than that of the right (north-eastern) bay (0.07 items/m3) due to the different levels of population and economic development on the left and right coasts. The ecological risk assessment showed generally low to medium risk from MPs pollution in Yueqing Bay, with higher ecological risk index (H) and potential ecological hazards (RI) of MPs polymers in water samples. These data emphasize the need for timely and effective action to reduce the contribution of intensive human activities to MPs pollution and provide information for further ecotoxicological studies, pollution control, and policy development of MPs.

Aquatic toxicity, ecological effects, human exposure pathways and health risk assessment of liquid crystal monomers.

Liquid crystal monomers (LCMs), one of the key materials for liquid crystal displays, have been considered as emerging pollutants in recent years. However, the environmental behaviors of LCMs have not yet been well investigated. The toxicity data of 1173 LCMs were calculated by integrated computational simulation methods in this study. It showed that 64.6% LCMs exhibited PBT (persistent, bioaccumulative, and toxic) properties. Based on the results, 1173 LCMs were identified as molecules possessing the highest level of acute toxicity to aquatic organisms. Among which, and a human health risk priority control list about LCMs was generated in this study, among which 435 were classified as requiring priority control LCMs. It was confirmed that LCMs could eventually accumulate in the human body along the aquatic food chain or penetrate the bloodstream through the dermis, thereby causing harm to health by identifying the exposure pathways of LCMs in humans. Additionally, the electronegativity of the side chain group of LCMs is the main factor causing toxicity differences; therefore, the LCMs containing halogens presented significant acute and chronic toxic effects. This study provided a more comprehensive understanding of LCMs for the public and scientific strategies for controlling LCMs.

Australians' perceptions of the potential effects of increased access to alcohol via autonomous delivery services: A multi-method study.

INTRODUCTION: Rapid technological change will affect how people access harmful products. For example, automated delivery services are forecast to provide fast and affordable product access options for those purchasing alcohol. Information about the potential impacts of such innovations on alcohol purchase and consumption behaviors is lacking. The aim of this study was to explore how consumers may respond to future scenarios where alcohol is available via a range of autonomous alcohol delivery options. METHODS: In a two-stage process, qualitative individual interviews (n = 100) and a quantitative online survey (n = 1078) were conducted with Australians aged 18+ years. Quotas were used to achieve national representation on key demographic variables. Participants were exposed to scenarios outlining how autonomous vehicles are likely to be used for alcohol deliveries in the future and asked to discuss their intentions to use such services and their perceptions of any impacts on alcohol consumption at a societal level. RESULTS: Automated alcohol deliveries were generally considered to be highly convenient and therefore likely to be popular. Around one-third (37%) of survey respondents reported an intention to use such automated alcohol delivery services once they are available and almost half (47%) expected overall levels of alcohol consumption to increase across the population. CONCLUSION: Given potential levels of uptake, careful consideration needs to be given to the development and implementation of appropriate policy and regulatory frameworks to minimize the increased risk of alcohol-related harm associated with enhanced alcohol availability resulting from the emerging availability of autonomous delivery services.

Molecular, biochemical and histological assessment in paraquat induced neurotoxicity: restoring role of nano-curcumin in an experimental study.

BACKGROUND: Paraquat is known to cause damage to various organs, including the brain. Although curcumin have anti-inflammatory and anti-oxidant properties, it is not yet clear how they relate to PQ-induced neurotoxicity. This study's objective was to compare the effects of curcumin and nanocurcumin on PQ-induced neurotoxicity in male rats. CONCLUSIONS: Treatment with curcumin and nano-curcumin improves brain function in PQ toxicity and nanocurcumin was more advantageous than ordinary curcumin.

Titanium modification using bioactive titanate layer with divalent ions and coordinated ciprofloxacin - Assessment of drug distribution using FT-IR imaging.

The paper presents a new method of titanium alloy (Ti6Al4V) modification using bioactive titanate layers containing various divalent ions (Ca2+, Mg2+, Sr2+, Zn2+) and surface-coordinated ciprofloxacin. Due to the coordination of ciprofloxacin (antibiotic) on the surface of the alloy, it has great application potential. In the paper, the influence of a given cation on the effectiveness of drug sorption was determined. The most effective cation was zinc and the least effective was calcium. The distribution of the antibiotic on the alloy surface was determined using FT-IR imaging. The antibiotic was evenly distributed on alloys modified with magnesium, strontium and zinc titanates. In the case of calcium titanate, the analysis could not be performed because the amount of the drug was too small. The release profiles of ciprofloxacin indicate that it can be released for as long as 3 h for strontium and zinc titanates. The biocompatibility of the obtained materials is indicated by the results of the BSA adsorption, and HA growth test. The obtained results confirm that the proposed modification can be used in the modification of titanium implants. The big advantage of this layer is that ciprofloxacin is coordinated on the surface of the material and thus will not be removed during the surgical procedure. The creation of this type of layer may in the future allow for fewer perioperative infections, and thus fewer complications.

Assessment of biogenic amines in commercial tuna fish: Influence of species, capture method, and processing on quality and safety.

This study investigated the influence of species, capture method, chilling, and freezing on the quality and variations in biogenic amines of Thunnus tonggol and Thunnus albacares. Samples were collected from different sources, including fisheries ports, cold rooms, and canning factories. The results indicated that canned fish had significantly higher levels of biogenic amines, especially histamine, compared to raw fish from ports and cold rooms. However, all canned samples complied with the FDA regulation of 50 mg/kg for histamine content. Canning led to a substantial increase in total amines, with histamine being the predominant amine in most groups. Putrescine was present in all samples, with the highest levels found in canned tuna made from frozen yellow-fin tuna captured using drift gillnetting. Canned samples also exhibited higher cadaverine levels compared to raw tuna. Storage conditions, capture method, and tuna species were found to significantly affect tuna quality and biogenic amines formation.

The first harmonised total diet study in Portugal: Vitamin D occurrence and intake assessment.

Vitamin D acts in calcium and phosphate homeostasis and also as an immunomodulatory hormone. To estimate the vitamin D intake by the 'adults' and 'elderly' Portuguese populations TDS methodology was used, since in the absence of skin UVB exposure, food and supplements are the only vitamin D sources. Vitamin D was quantifiable in 78 (24 from the fish group) of the 164 TDS samples. Sea bream contained the most vitamin D (13.8 µg/100 g), followed by plaice (9.2 µg/100 g). MCRA software (semi-probabilistic approach) was used to estimate the median vitamin D intake that ranged between 2.47 ('adults' 'males') - 1.45 ('elderly' 'females') µg/day, well below the Dietary Reference Values (5-15 µg/day). Plaice, sea bream and sardine were the main contributors to intake. A prevalence of 94% inadequate vitamin D intake for 'adults' and 'elderly' was found based on the estimated average requirement of 10 µg/day.

Assessment of phthalate esters in packaged fruit juices sold in the Turkish market and their implications on human health risk.

The objective of this study was to analyze the concentration of PAEs and conduct a risk assessment focusing on their potential non-carcinogenic and carcinogenic effects as endocrine-disrupting chemicals. In this research, a method of quantifying eight PAEs in fruit juice samples was established through the utilization of dispersive solid-phase extraction (dSPE) and Liquid Chromatography coupled with Tandem Mass Spectrometry (LC-MS/MS). While he method recoveries for the samples ranged from 92.21 % to 96.74 %, the RSDs for the eight analytes in the samples ranged from 4.22 % to 6.64 %. Additionally, LODs ranging from 0.34 to 1.88 ng/mL and LOQs ranging from 1.17 to 6.27 ng/mL were determined. Utilizing this methodology, the assessment of PAE concentrations was carried out in 48 fruit juice samples. The results showed that PAEs were detectable in the fruit juice samples, but all tested compounds remained below the specific migration limits set by the EU (30 mg/kg for BBP, 1.5 mg/kg for DEHP, 0.3 mg/kg for DBP, and 9 mg/kg for DINP and DIDP). Among the analyzed PAEs, DINP (26.94 ± 3.25 ng/mL), DEP (7.07 ± 7.59 ng/mL), and DEHP (3.48 ± 1.02 ng/mL) were found to be the most prevalent plasticizers in the fruit juices. Health risk assessments were conducted by calculating Hazard Quotient (HQ) and Hazard Index (HI) values based on the consumption patterns of different age groups. The findings indicated that the values for DEHP, DINP, DEP, and DBP were all below 1, indicating negligible non-carcinogenic health risks associated with the consumption of fruit juices across all age groups. Moreover, the dietary intake levels of each PAE were found to be below the values established by the EFSA for TDI.