[Determination of carbadox metabolites, quinoxaline-2-carboxylic acid and desoxycarbadox, in swine muscle and liver by liquid chromatography/mass spectrometry].
Shokuhin Eiseigaku Zasshi
; 45(3): 135-40, 2004 Jun.
Article
em Ja
| MEDLINE
| ID: mdl-15468932
ABSTRACT
A sensitive and selective method using liquid chromatography-electrospray mass spectrometry (LC-ESI-MS) for the determination of carbadox metabolites, quinoxaline-2-carboxylic acid (QCA) and desoxycarbadox (Desoxy-CDX), in swine muscle and liver has been developed. The LC separation was performed on a Cadenza CD-C18 column (10 cm x 2 mm i.d.) with a gradient system of 0.01% acetic acid-acetonitrile as the mobile phase at a flow rate of 0.2 mL/min. Negative ionization produced the [M-H]- molecular ion of QCA. On the other hand, the positive mode produced the [M+H]+ ion of Desoxy-CDX. The calibration graphs for QCA and Desoxy-CDX were rectilinear from 0.01 to 0.5 ng with selected ion monitoring (SIM). The drugs were extracted with 0.3% metaphosphoric acid-methanol (73), and the extracts were cleaned up on an Oasis HLB cartridge (60 mg) and by liquid-liquid extraction. The recoveries of QCA and Desoxy-CDX from swine muscle and liver fortified at 2.5 and 5 ng/g were 70.2-86.3%, and the detection limits were 1 ng/g for both drugs.
Buscar no Google
Temas:
ECOS
/
Estado_mercado_regulacao
Bases de dados:
MEDLINE
Assunto principal:
Quinoxalinas
/
Espectrometria de Massas
/
Carbadox
/
Resíduos de Drogas
/
Cromatografia Líquida de Alta Pressão
/
Análise de Alimentos
/
Carne
/
Anti-Infecciosos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Ja
Revista:
Shokuhin Eiseigaku Zasshi
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Japão