A reliable and inexpensive EasyCD4 assay for monitoring HIV-infected individuals in resource-limited settings.
J Acquir Immune Defic Syndr
; 43(1): 23-6, 2006 Sep.
Article
em En
| MEDLINE
| ID: mdl-16885780
ABSTRACT
Serial measurements of absolute CD4+ T-lymphocyte counts are required to initiate and gauge response to therapy and monitor disease progression. Hence, there is an urgent need to evaluate the accuracy and validity of low-cost CD4+ T-cell count assays. Tripotassium EDTA blood specimens from HIV-infected individuals were studied using a novel flow cytometric assay (EasyCD4 assay; Guava Technologies, Hayward, CA) in comparison with standard flow cytometry (FACSCount; Becton Dickinson Immunocytometry Systems, San Jose, CA). The sensitivity, specificity value by EasyCD4 assay in enumerating absolute CD4+ T-cell counts of less than 200 cells/microL were 95% and 100%, respectively. Bland-Altman analysis showed close agreement, with the EasyCD4 assay yielding CD4+ T-cell counts a mean difference of -26 cells/microL (95% confidence interval, -96 to 44 cells/microL) higher than by flow cytometry. Our data suggest that EasyCD4 assay could be a useful alternative assay to conventional flow cytometry, may be appropriate for use in resource-limited settings.
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Temas:
ECOS
/
Aspectos_gerais
/
Financiamentos_gastos
Bases de dados:
MEDLINE
Assunto principal:
Infecções por HIV
/
Contagem de Linfócito CD4
Tipo de estudo:
Diagnostic_studies
/
Health_economic_evaluation
Limite:
Adolescent
/
Adult
/
Child
/
Humans
/
Middle aged
País/Região como assunto:
Asia
Idioma:
En
Revista:
J Acquir Immune Defic Syndr
Assunto da revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Índia