Development of a novel biofilm continuous culture method for simultaneous assessment of architecture and gaseous metabolite production.
Appl Environ Microbiol
; 74(17): 5429-35, 2008 Sep.
Article
em En
| MEDLINE
| ID: mdl-18606794
ABSTRACT
The way that gaseous metabolite production changes along with biofilm architecture development is poorly understood. To address this question, we developed a novel flow reactor biofilm culture method that allows for simultaneous assessment of gaseous metabolite production and architecture visualization. In this report, we establish the utility of this method using denitrification by Pseudomonas aeruginosa biofilms as a model system. Using this method, we were able to collect and analyze gaseous metabolites produced by denitrification and also visualize biofilm architecture in a nondestructive manner. Thus, we propose that this novel method is a powerful tool to investigate potential relationships between biofilm architecture and the gas-producing metabolic activity of biofilms, providing new insights into biofilm ecology.
Texto completo:
1
Temas:
ECOS
/
Aspectos_gerais
Bases de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
/
Biofilmes
/
Reatores Biológicos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Japão