Practical capability and cost effectiveness of a DNA pool-based genome-wide association study using BovineSNP50 array in a cattle population.
Anim Sci J
; 83(11): 719-26, 2012 Nov.
Article
em En
| MEDLINE
| ID: mdl-23126324
Genome-wide association mapping for complex traits in cattle populations is a powerful, but expensive, selection tool. The DNA pooling technique can potentially reduce the cost of genome-wide association studies. However, in DNA pooling design, the additional variance generated by pooling-specific errors must be taken into account. Therefore, this study aimed to investigate factors such as: (i) the accuracy of allele frequency estimation; (ii) the magnitude of errors in pooling construction and in the array; and (iii) the effect of the number of replicate arrays on P-values estimated by a genome-wide association study. Results showed that the Illumina correction method is the most effective method to correct the allele frequency estimation; pooling errors, especially array variance, should be taken into account in DNA pooling design; and the risk of a type I error can be reduced by using at least two replicate arrays. These results indicate the practical capability and cost-effectiveness of pool-based genome-wide association studies using the BovineSNP50 array in a cattle population.
Texto completo:
1
Temas:
ECOS
/
Aspectos_gerais
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Financiamentos_gastos
Bases de dados:
MEDLINE
Assunto principal:
DNA
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Bovinos
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Análise de Sequência com Séries de Oligonucleotídeos
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Polimorfismo de Nucleotídeo Único
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Estudo de Associação Genômica Ampla
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Pool Gênico
Tipo de estudo:
Health_economic_evaluation
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Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Anim Sci J
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Japão