Assessment of umbilical cord tissue as a source of mesenchymal stem cell/endothelial cell mixtures for bone regeneration.
Regen Med
; 8(5): 569-81, 2013 Sep.
Article
em En
| MEDLINE
| ID: mdl-23998751
ABSTRACT
AIM:
To enumerate and characterize mesenchymal stem cells (MSCs) and endothelial cells (ECs) in umbilical cord (UC) tissue digests. MATERIALS &METHODS:
Cultured UC cells were characterized phenotypically, and functionally by using 48-gene arrays. Native MSCs and ECs were enumerated using flow cytometry.RESULTS:
Compared with bone marrow (BM) MSCs, UC MSCs displayed significantly lower (range 4-240-fold) basal levels of bone-related transcripts, but their phenotypes were similar (CD73âº, CD105âº, CD90âº, CD45â» and CD31â»). UC MSCs responded well to osteogenic induction, but day 21 postinduction levels remained below those achieved by BM MSCs. The total yield of native UC MSCs (CD90âº, CD45â» and CD235αâ») and ECs (CD31âº, CD45â» and CD235αâ») exceeded 150 and 15 million cells/donation, respectively. Both UC MSCs and ECs expressed CD146.CONCLUSION:
While BM MSCs are more predisposed to osteogenesis, UC tissue harbors large numbers of MSCs and ECs; such minimally manipulated 'off-the-shelf' cellular mixtures can be used for regenerating bone in patients with compromised vascular supply.
Texto completo:
1
Temas:
ECOS
/
Aspectos_gerais
Bases de dados:
MEDLINE
Assunto principal:
Cordão Umbilical
/
Regeneração Óssea
/
Células Endoteliais
/
Células-Tronco Mesenquimais
Limite:
Humans
Idioma:
En
Revista:
Regen Med
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Reino Unido