Bacterial Expression and HTS Assessment of Soluble Epoxide Hydrolase Phosphatase.
J Biomol Screen
; 21(7): 689-94, 2016 Aug.
Article
em En
| MEDLINE
| ID: mdl-27009944
ABSTRACT
Soluble epoxide hydrolase (sEH) is a bifunctional enzyme that possesses an epoxide hydrolase and lipid phosphatase activity (sEH-P) at two distinct catalytic domains. While the physiological role of the epoxide hydrolase domain is well understood, the consequences of the phosphatase activity remain unclear. Herein we describe the bacterial expression of the recombinant N-terminal domain of sEH-P and the development of a high-throughput screening protocol using a sensitive and commercially available substrate fluorescein diphosphate. The usability of the assay system was demonstrated and novel inhibitors of sEH-P were identified.
Palavras-chave
Texto completo:
1
Temas:
ECOS
/
Aspectos_gerais
Bases de dados:
MEDLINE
Assunto principal:
Monoéster Fosfórico Hidrolases
/
Inibidores Enzimáticos
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Epóxido Hidrolases
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Ensaios de Triagem em Larga Escala
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Biomol Screen
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Alemanha