Characterization of a functional recombinant human creatine kinase-MB isoenzyme prepared by tandem affinity purification from Escherichia coli.

Zou, Lihui; Su, Wen; Wang, Meng; Huang, Wei; Zhao, Haijian; Zhang, Enyi; Jin, Junhua; Xu, Hongtao; Xiao, Fei

Zou, Lihui; Su, Wen; Wang, Meng; Huang, Wei; Zhao, Haijian; Zhang, Enyi; Jin, Junhua; Xu, Hongtao; Xiao, Fei.

Afiliação

Zou L; The MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing, 100730, People's Republic of China.
Su W; Department of Internal Neurology, Beijing Hospital, National Center of Gerontology, Beijing, 100730, People's Republic of China.
Wang M; Department of Laboratory Medicine, Beijing Hospital, National Center of Gerontology, Beijing, 100730, People's Republic of China.
Huang W; The MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing, 100730, People's Republic of China.
Zhao H; National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing, 100730, People's Republic of China.
Zhang E; The MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing, 100730, People's Republic of China.
Jin J; The MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing, 100730, People's Republic of China.
Xu H; Department of Laboratory Medicine, Beijing Hospital, National Center of Gerontology, Beijing, 100730, People's Republic of China.
Xiao F; The MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing, 100730, People's Republic of China. xiaofei@bjhmoh.cn.

Appl Microbiol Biotechnol ; 101(14): 5639-5644, 2017 Jul.

Article em En | MEDLINE | ID: mdl-28432439

Assuntos

Cromatografia de Afinidade/métodos; Creatina Quinase Forma MB/genética; Creatina Quinase Forma MB/metabolismo; Escherichia coli/genética; Cromatografia de Afinidade/economia; Ensaios Enzimáticos Clínicos; Creatina Quinase Forma MB/isolamento & purificação; Estabilidade Enzimática; Escherichia coli/enzimologia; Humanos; Proteínas Recombinantes/isolamento & purificação; Proteínas Recombinantes/metabolismo; Padrões de Referência

Palavras-chave

CK-MB; Enzyme activity; Gene recombination; Protein expression; Tandem affinity purification

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Texto completo: 1 Temas: ECOS / Aspectos_gerais Bases de dados: MEDLINE Assunto principal: Cromatografia de Afinidade / Escherichia coli / Creatina Quinase Forma MB Tipo de estudo: Evaluation_studies Limite: Humans Idioma: En Revista: Appl Microbiol Biotechnol Ano de publicação: 2017 Tipo de documento: Article

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