Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies.
Methods Enzymol
; 632: 39-54, 2020.
Article
em En
| MEDLINE
| ID: mdl-32000907
ABSTRACT
Dendritic cells (DCs) are specialized antigen presenting cells (APCs) able to intake and crosspresent antigens (Ags) on major histocompatibility complex (MHC) class I and II molecules to T cells thus initiating primary and memory immune responses. DC-mediated Ag uptake and crosspresentation represent crucial steps toward cancer recognition and eventually elimination. Cytofluorometry is a standardized procedure to study phagocytosis. By fast and reproducible single cell measurements, flow cytometry allows for simultaneous biochemical and functional analyses of Ag intake. In this chapter, we discuss a two-color flow cytometric analysis of DC-mediated uptake of apoptotic bodies. We also show data on the adjuvanticity of Type-I-interferons (Type-I-IFNs) during Ag retention as we offer a guideline and a range of advice on sample preparation and acquisition.
Palavras-chave
Texto completo:
1
Temas:
ECOS
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Aspectos_gerais
Bases de dados:
MEDLINE
Assunto principal:
Células Dendríticas
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Citometria de Fluxo
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Vesículas Extracelulares
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Neoplasias
Limite:
Animals
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Humans
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Itália