Assessment of total immunoreactive lactoferrin in hematopoietic cells using flow cytometry.
J Immunol Methods
; 108(1-2): 159-70, 1988 Apr 06.
Article
em En
| MEDLINE
| ID: mdl-3280685
ABSTRACT
Cell-associated lactoferrin (Lf) was analyzed using a new method involving cell permeabilization, indirect immunofluorescence staining, and flow cytometry. Statistical techniques to evaluate the results for percentage of positive cells, relative fluorescence and homogeneity of Lf distribution were also devised. Most normal adult neutrophils (97.1 +/- 0.3% (SEM), range 92.7-99.6%, n = 41) had brilliant fluorescence homogeneously distributed among the cells. There was significantly greater homogeneity of neutrophil Lf distribution in post-menopausal than pre-menopausal females. In chronic myelogenous leukemia (n = 13) and cord blood (n = 7), fractions of Lf-positive neutrophils were decreased (77.3 +/- 7.5%, range 13.3-96.3%; 71.4 +/- 9.3, range 32.0-95.6%, respectively). Normal monocyte-rich isolates had moderate fluorescence (28.7 +/- 3.6%, range 9.3-76.8%, n = 22). Among blood lymphocyte-rich preparations, 13.1 +/- 1.3% of cells had weak positivity (range 4.9-26.6%, n = 19); monoclonal B and T lymphocytes had similar parameters. No other cells had detectable Lf. Our results were significantly correlated with those obtained manually (r = 0.98, P less than 0.001), and are consistent with Lf quantity and distribution determined using other methods.
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Temas:
ECOS
/
Aspectos_gerais
Bases de dados:
MEDLINE
Assunto principal:
Células Sanguíneas
/
Imunofluorescência
/
Citometria de Fluxo
/
Lactoferrina
/
Lactoglobulinas
Limite:
Adult
/
Animals
/
Female
/
Humans
/
Male
/
Newborn
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1988
Tipo de documento:
Article