The potential of organoids in toxicologic pathology: role of toxicologic pathologists in in vitro chemical hepatotoxicity assessment.
J Toxicol Pathol
; 35(3): 225-235, 2022 Jul.
Article
em En
| MEDLINE
| ID: mdl-35832897
ABSTRACT
The development of in vitro toxicity assessment methods using cultured cells has gained popularity for promoting animal welfare in animal experiments. Herein, we briefly discuss the current status of hepatoxicity assessment using human- and rat-derived hepatocytes; we focus on the liver organoid method, which has been extensively studied in recent years, and discuss how toxicologic pathologists can use their knowledge and experience to contribute to the development of in vitro chemical hepatotoxicity assessment methods for drugs, pesticides, and chemicals. We also propose how toxicological pathologists should assess toxicity regarding the putative distribution of undifferentiated and differentiated cells in the organoid when liver organoids are observed in hematoxylin and eosin-stained specimens. This was done while considering the usefulness and limitations of in vitro studies for toxicologic pathology assessment.
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Temas:
ECOS
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Aspectos_gerais
Bases de dados:
MEDLINE
Idioma:
En
Revista:
J Toxicol Pathol
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Japão