Transmission electron microscopy assessment of a novel method for isolating pure exosomes from serum.
Biotech Histochem
; 98(6): 391-395, 2023 Nov.
Article
em En
| MEDLINE
| ID: mdl-37125533
ABSTRACT
Serum exosomes frequently are used for liquid biopsy. Serum exosomes normally are isolated using ultracentrifugation; however, ultracentrifugation is time-consuming, labor intensive and requires a high-speed centrifuge. Many commercial kits use a precipitation-based method; however, this process can result in substantial contamination. We developed a new method to isolate pure serum exosomes. We isolated serum exosomes using precipitation, extracted them using acetone, then isolated them again by precipitation. We used transmission electron microscopy (TEM) to examine the morphology of serum exosomes. TEM indicated that our isolated exosomes were pure with typical morphology and with a size ranging from 40 to 150 nm. Flow cytometry revealed expression of exosome markers, CD63, CA81 and CD9. Our double precipitation method enables ready extraction of pure exosomes from serum. Our double precipitation method simplifies detection of serum exosomal biomarkers for diagnosis and prognosis of disease.
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Texto completo:
1
Temas:
ECOS
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Aspectos_gerais
Bases de dados:
MEDLINE
Assunto principal:
Exossomos
Idioma:
En
Revista:
Biotech Histochem
Assunto da revista:
HISTOCITOQUIMICA
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China