ABSTRACT
INTRODUCTION: Kidney transplantation is a definitive treatment for end-stage renal disease. It is associated with improved life expectancy and quality of life. One of the most common complications following kidney transplantation is graft rejection. To our knowledge, no previous study has identified rejection risk factors in kidney transplant recipients in Saudi Arabia. Therefore, this study aimed to determine the specific risk factors of graft rejection. METHODS: A multicenter case-control study was conducted at four transplant centers in Saudi Arabia. All adult patients who underwent a renal transplant between January 1, 2015 and December 31, 2021 were screened for eligibility. Included patients were categorized into two groups (cases and control) based on the occurrence of biopsy-proven rejection within 2 years. The primary outcome was to determine the risk factors for rejection within the 2 years of transplant. Exact matching was utilized using a 1:4 ratio based on patients' age, gender, and transplant year. RESULTS: Out of 1,320 screened renal transplant recipients, 816 patients were included. The overall prevalence of 2-year rejection was 13.9%. In bivariate analysis, deceased donor status, the presence of donor-specific antibody (DSA), intraoperative hypotension, Pseudomonas aeruginosa, Candida, and any infection within 2 years were linked with an increased risk of 2-year rejection. However, in the logistic regression analysis, the presence of DSA was identified as a significant risk for 2-year rejection (adjusted OR: 2.68; 95% CI: 1.10, 6.49, p = 0.03). Furthermore, blood infection, infected with Pseudomonas aeruginosa or BK virus within 2 years of transplant, were associated with higher odds of 2-year rejection (adjusted OR: 3.10; 95% CI: 1.48, 6.48, p = 0.003, adjusted OR: 3.23; 95% CI: 0.87, 11.97, p = 0.08 and adjusted OR: 2.76; 95% CI: 0.89, 8.48, p = 0.07, respectively). CONCLUSION: Our findings emphasize the need for appropriate prevention and management of infections following kidney transplantation to avoid more serious problems, such as rejection, which could significantly raise the likelihood of allograft failure and probably death. Further studies with larger sample sizes are needed to investigate the impact of serum chloride levels prior to transplant and intraoperative hypotension on the risk of graft rejection and failure.
ABSTRACT
Introduction Forensic science has continually evolved, with innovations in DNA analysis techniques playing a pivotal role in improving the accuracy and reliability of criminal investigations. Short tandem repeats (STRs) have been a game-changer in forensic science, offering unique genetic markers to pinpoint individual identities. This study explores the application of STR DNA analysis to an unconventional source: coffee cups. Materials and methods In a study involving 16 unrelated, healthy individuals aged 26 to 32, DNA was investigated from coffee cups and mouth cavities, comparing the results to a previous coffee cup DNA study. Buccal swabs provided reference samples, air-dried for 10 minutes, and stored at 20°C. DNA quantification revealed a significant decrease in coffee cup samples (0.015 to 0.049 ng/µL) compared to the reference, with no DNA in negative controls. Some STR loci displayed inhibition and lower heterozygosity in the research samples. Ethical approval (REC 53-592) and adherence to the Declaration of Helsinki ensured ethical standards. This research highlights potential challenges in obtaining efficient DNA profiles from various sources. Results There was a significant variation in DNA concentrations among the different cup types, with ceramic cups yielding the highest concentrations. Moreover, the DNA profiling outcomes ranged from full profiles, which allow for precise individual identification, to partial profiles, which pose challenges for conclusive matches. These findings have profound implications for forensic science. The study demonstrates the potential of coffee cups as sources of DNA evidence in criminal investigations, even if partial profiles are obtained. Such evidence can assist in suspect identification, victim profiling, and corroborating witness statements. Conclusion This study highlights the application of STR DNA analysis for coffee cups, opening new avenues for forensic investigations and highlighting the need for continued research and development in this exciting field. This observation aligns with existing research on DNA recovery from various surfaces, and further research is warranted to refine the methodology, consider usage patterns, and address ethical and legal concerns regarding the collection and use of DNA evidence from common objects.
ABSTRACT
BACKGROUND: Vaccination is the best weapon to prevent any disease, especially pandemics like COVID-19, and building herd immunity is the best way to control the disease's spread. On one side, vaccine availability is important, and on the other, its successful distribution is faced with difficulty in a wide geographical area. Availability and distribution go hand in hand, which is the public health challenge. Vaccines are taking over, clearing up concerns about vaccination and making the public ready. The high-risk public should receive vaccines without resistance, which is of utmost importance. Unvaccinated older adults are at higher risk for COVID-19 infection, morbidity, and mortality. The reasons why older people who have not been vaccinated against COVID-19 do not want to receive vaccines when they are available include a lack of knowledge about COVID-19 vaccination and listening to incorrect sources of information. With this background, this study aimed to assess the knowledge, attitudes, and concerns of unvaccinated older adults in the Aseer Region, Saudi Arabia. METHODS: A community-based cross-sectional study was planned to find out the knowledge and attitudes of the elderly in the Assir Region, Saudi Arabia. A total of 434 unvaccinated elderly persons were recruited randomly from the list of elderly (>60 years) who were unvaccinated. The data was gathered using a structured interview schedule in Arabic. RESULTS: Out of 434 participants, more than half (54.8%) were male. Most of the participants were Saudi citizens (91.5%). Of the participants, nearly one-fifth (19.8%) of them had a previous history of COVID-19 infection. The participants' main sources of information about vaccination against COVID-19 were the mass media (41.9%), followed by word of mouth from friends, families, or neighbors (41.7%), and social media (16.4%). More than three-fourths of them (85.7%) had poor knowledge regarding the COVID-19 vaccination. The participants' poor knowledge grades were mainly among those aged >80 years, illiterate, unemployed participants, and current smokers. Those participants relying on social media had the highest number of concerns (6.663.21) regarding the safety and efficacy of vaccines. CONCLUSIONS: Most participants have poor knowledge, and their knowledge of COVID-19 vaccination is limited. Participants whose main source of knowledge is the mass media need to intensify their education activities in the mass media. Social media, whose primary source of information is social media, has the greatest number of issues that require immediate attention. Social media content must be scanned, and misinformation needs to be addressed.
ABSTRACT
INTRODUCTION: Cataract is the most prevalent age-related eye disease and the most curable cause of adult visual impairment. The aim of this study was to assess the cataract disease rate and knowledge regarding its definition, symptoms, risk factors, prognosis, and treatment. METHODS: A community-based cross-sectional study of 600 randomly selected people aged 18 and up was conducted from May 2022 to August 2022, among adults. Participants were given an online survey via social media that included items testing their knowledge of cataracts. RESULTS: Study participants had a 3.4% cataract previous exposure rate. Four hundred forty-eight (75.9%) participants had good knowledge about cataracts. Participants with higher educational levels were more likely to correctly answer questions about different aspects of knowledge about cataracts. CONCLUSION: Future studies should focus on improving awareness about cataracts. Risk factors, complications, treatment options, complications of cataract surgery, and regular follow-ups should be explained to patients. Through routine eye checks, early detection and treatment of this condition will be better understood.