ABSTRACT
Multivariate statistical techniques, cluster analysis (CA) and principal component analysis (PCA) were applied to the data on water quality of Manchar Lake (Pakistan), generated during 2005-06, with monitoring at five different sites for 36 parameters. This study evaluated and interpreted complex water quality data sets and apportioned of pollution sources to get better information about water quality and to design a monitoring network. The chemical correlations were observed by PCA, which were used to classify the samples by CA, based on the PCA scores. Three significant sampling locations--(sites 1 and 2), (site 4) and (sites 3 and 5)--were detected on the basis of similarity of their water quality. The results revealed that the major causes of water quality deterioration were related to inflow of effluent from industrial, domestic, agricultural and saline seeps into the lake at site 1 and also resulting from people living in boats and fishing at sites 2 and 3.
Subject(s)
Environmental Monitoring/methods , Fresh Water/chemistry , Risk Assessment/methods , Water Pollutants/analysis , Water Supply/analysis , Water Supply/standards , Cluster Analysis , Environmental Monitoring/statistics & numerical data , Pakistan , Principal Component Analysis , Risk Assessment/statistics & numerical dataABSTRACT
BACKGROUND: We report cascade testing of a large Pakistani family for beta-thalassemia alleles. The family was still practicing consanguineous marriages and was at risk of having more affected births. OBJECTIVE: The objective of this study was to show that identification of disease carriers in families with index cases in order to create awareness about disease and provide genetic counseling would result in reduction of the frequency of beta-thalassemia in Pakistan. METHODS: In this large family with an index case, 27 available living members were tested for beta-thalassemia. Carriers of the disease were detected by measuring hemoglobin indices, and amplification refractory mutation system polymerase chain reaction was used for mutation analysis. Genetic counseling was provided to members of this family. RESULTS: There were already 3 marriages between the carrier members and 1 between a carrier and noncarrier in this large family; 12 (44.4%) members were found to carry the mutant gene, representing a very high carrier rate compared to the 5.4% carrier frequency of beta-thalassemia in the general population of Pakistan. The family was counseled for prevention of affected births. The initially reluctant family gradually became cooperative and seriously attended the genetic counseling sessions. CONCLUSION: Cascade testing is more practical than general population screening in a country with limited health facilities where consanguineous marriages are practiced. This report emphasizes the need of extensive testing within families with index cases to identify the carriers of beta-thalassemia in order to reduce disease occurrence through awareness and genetic counseling.
Subject(s)
Genetic Testing/methods , beta-Thalassemia/diagnosis , beta-Thalassemia/genetics , Consanguinity , DNA Mutational Analysis , Family Health , Female , Gene Frequency , Heterozygote , Humans , Male , Mutation , Pakistan , Pedigree , Risk , beta-Thalassemia/ethnology , beta-Thalassemia/prevention & controlABSTRACT
The mobility, availability and persistence of heavy metals (Cd, Cr, Cu, Ni, Pb and Zn) in contaminated lake sediment samples were evaluated by means of sequential extraction scheme, proposed by the Community Bureau of Reference protocol (BCR). The metal content in the extracts was measured by atomic absorption spectrometry. The precision and accuracy of the proposed procedure were evaluated by using a certified reference material BCR 701. The maximum recoveries for heavy metals (HMs) were observed for all three steps of BCR protocol at 32h total shaking period instead of previously reported 51h, with p>0.05. The lixiviation tests (DIN 38414-S4) were used to evaluate the leaching of HMs from sediment samples and it was observed that levels of water extractable HMs were low as compared to those values obtained by acid-soluble fraction of the BCR protocol.
Subject(s)
Geologic Sediments/analysis , Metals, Heavy/analysis , Water Pollutants, Chemical/analysis , Water Pollution, Chemical/analysis , Acids , Fresh Water/analysis , Indicators and Reagents , Microwaves , Reference Standards , Spectrophotometry, AtomicABSTRACT
Simian immunodeficiency virus (SIV) infection of rhesus monkeys provides an excellent model of the central nervous system (CNS) consequences of HIV infection. To discern the relationship between viral load and abnormalities induced in the CNS by the virus, we infected animals with SIV and later instituted antiviral treatment to lower peripheral viral load. Measurement of sensory-evoked potentials, assessing CNS neuronal circuitry, revealed delayed latencies after infection that could be reversed by lowering viral load. Cessation of treatment led to the reappearance of these abnormalities. In contrast, the decline in general motor activity induced by SIV infection was unaffected by antiviral treatment. An acute increase in the level of the chemokine monocyte chemoattractant protein-1 (MCP-1) was found in the cerebrospinal fluid (CSF) relative to plasma in the infected animals at the peak of acute viremia, likely contributing to an early influx of immune cells into the CNS. Examination of the brains of the infected animals after return of the electrophysiological abnormalities revealed diverse viral and inflammatory findings. Although some of the physiological abnormalities resulting from SIV infection can be at least temporarily reversed by lowering viral load, the viral-host interactions initiated by infection may result in long-lasting changes in CNS-mediated functions.
Subject(s)
Antiviral Agents/therapeutic use , Brain/physiopathology , Movement Disorders/drug therapy , Simian Acquired Immunodeficiency Syndrome/drug therapy , Animals , Blood-Brain Barrier , Brain/drug effects , Brain/virology , Evoked Potentials, Auditory, Brain Stem/drug effects , Macaca mulatta , Motor Activity/drug effects , Simian Acquired Immunodeficiency Syndrome/physiopathology , Simian Acquired Immunodeficiency Syndrome/virology , Simian Immunodeficiency Virus/isolation & purificationABSTRACT
A simple and rapid non-chromatographic method was developed to determine methylmercury (MeHg) and inorganic mercury (iHg) levels in muscles tissues of 10 freshwater fish species. The MeHg and iHg were determined by cold vapour atomic absorption spectrometry after alkaline wet digestion of samples. The digested samples were reduced sequentially with stannous chloride and sodium tetrahydroborate for iHg and MeHg, respectively. Parameters such as carrier gas flow rate (argon), volume of oxidizing and potassium persulphate solutions were investigated in detail. The accuracy of the technique was evaluated by using certified reference material (DORM-2) and spiking the both Hg species in muscles tissue of a fish. The limits of detection were 0.117 and 0.133 µg kg(-1) for MeHg and iHg, respectively. The concentrations of MeHg and iHg in muscles tissues of ten fish species were found in the range of (28.4-56.3) and (3.01-8.11) µg kg(-1), respectively.
Subject(s)
Mercury/analysis , Methylmercury Compounds/analysis , Muscle, Skeletal/chemistry , Spectrophotometry, Atomic/methods , Animals , Fishes , Water Pollutants, Chemical/analysisABSTRACT
Cloud point extraction method has been developed for preconcentration of trace quantities of zinc (Zn) in aqueous extract of medicinal plants and blood samples of liver cancer patients using flame atomic absorption spectrometry. The Zn in aqueous extracts of medicinal plants (MPs) was complexed with 2-methyl-8-hydroxyquinoline (quinaldine) and 1-(2-pyridylazo)-2-naphthol (PAN) separately and entrapped in a non-ionic surfactant Triton X-114. After centrifugation, the surfactant-rich phase was diluted with 0.25mL acidic ethyl alcohol. The multivariate strategy was applied to estimate the optimum values of experimental variables (pH, time temperature, ligands and surfactant concentrations). Interactions between analytical factors and their optimal levels were investigated by two level factorial designs. Student's t-test on the results of factorial design with 16 runs for Zn extraction, demonstrated that the factors, ligands concentrations, pH and temperature were statistically significant. The accuracy was assessed by analysis of certified reference materials, namely, BCR 101 (spruce needles), Clincheck control-lyophilized human whole blood. Enhancement factor of 30 and 26 were achieved for the preconcentration of Zn by 2-methyl-8-hydroxyquinoline (L1) and 1-(2-pyridylazo)-2-naphthol (L2), respectively. The relative standard deviation for six replicate determinations of Zn at 10µg/L level using 2-methyl-8-hydroxyquinoline (L1) and 1-(2-pyridylazo)-2-naphthol (L2) were <4% and >5%, respectively.
Subject(s)
Liquid-Liquid Extraction/methods , Liver Neoplasms/blood , Plant Extracts/chemistry , Plants, Medicinal/chemistry , Spectrophotometry, Atomic/methods , Zinc/analysis , Adult , Female , Humans , Middle Aged , Plant Extracts/isolation & purification , Zinc/blood , Zinc/isolation & purificationABSTRACT
The chemical constituents in medicinal plants (MPs), including elements, are partially responsible for their medicinal and nutritional properties as well as toxic effects. This research aimed to monitor selenium (Se) contents in aqueous extract of MPs used for treatment of cancer and different diseases. In present work the Se in MPs was extracted in aqueous media by microwave-assisted (ME) and conventional extraction (CE) methods. The total and residual Se in MPs were determined, prior to microwave-assisted acid digestion. The Se in aqueous extracts and digests were analysed by electrothermal atomic absorption spectrometry. The optimum extractable Se in MPs were obtained by ME and CE, required 2 and 40 min, respectively. Precision and accuracy of the methodologies were checked by standard addition method. The Se contents in aqueous extract of MPs were found in the range of 1.09-2.23 µg/g corresponding to 21-33% of total Se contents. The daily intake of Se from aqueous extract of MPs as recommended by herbalist (10 g of plant material) was found in the range of 20-40% of daily requirement.
Subject(s)
Antioxidants/analysis , Neoplasms/drug therapy , Plants, Medicinal/chemistry , Selenium/analysis , Calibration , Diet , Dietary Supplements , Indicators and Reagents , Microwaves , Plant Extracts/analysis , Spectrophotometry, AtomicABSTRACT
In this study, the contents of total mercury (Hg) present in poultry feed, tissues of broiler chicken and manure were assessed. For this purpose, chicken feeds (five brands), different tissues of broiler chicken of two age groups (1-3 and 4-6 week) and manure samples were collected from five commercial poultry farms of Hyderabad, Pakistan. The Hg concentrations in feeds, chicken tissues (leg, muscle, liver and heart) and manure samples were determined by CVAAS, prior to microwave assisted acid digestion in closed vessels. For validation, a certified reference material, DORM-2 was used. The limit of detection and quantitation were 0.117 and 0.382 microg/kg, respectively The Hg concentration in different chicken feed were found in the range of 8.57-16.5 microg/kg. The concentration of Hg in chicken tissues were found in the range of 2.54-5.54 microg/kg (liver), 1.27-3.86 microg/kg (muscles) and 2.13-3.27 microg/kg (heart). The bioaccumulation factors (BAF) for Hg in different tissues were found in the range of 0.092-0.269. The obtained data shows the high correlation coefficient between feed and manure, while low r-values were obtained between Hg levels in feed and tissues of broiler chicken of two age groups.
Subject(s)
Animal Feed/analysis , Manure/analysis , Mercury/analysis , Spectrophotometry, Atomic/methods , Animals , Chickens , Mercury/pharmacokineticsABSTRACT
A cloud point extraction (CPE) method has been developed for the determination of total mercury (Hg) in different tissues of broiler chicken by cold vapor atomic absorption spectrometry (CVAAS). The broiler chicken tissues (leg, breast, liver and heart) were subjected to microwave assisted digestion in a mixture of nitric acid and hydrogen peroxide (2:1 ratio), prior to preconcentration by CPE. Various parameters such as the amount of ammonium O,O-diethyldithiophosphate (DDTP), concentrations of Triton X-114, equilibrium temperature, time and centrifugation have been studied in order to find the best conditions for the determination of mercury. For validation of proposed method a certified reference material, DORM-2 was used. No significant difference p>0.05 was observed between the experimental results and the certified values of CRM (paired t-test). The limit of detection and quantitation obtained under the optimal conditions were 0.117 and 0.382 microg/kg, respectively. The accumulation of Hg in different tissues were found in the order of, liver>muscles>heart. The concentration of Hg in chicken tissues were found in the range of 1.57-2.75, 1.40-2.27, 1.55-4.22, and 1.39-2.61 microg/kg in leg, breast, liver and heart, respectively.
Subject(s)
Chickens/metabolism , Mercury/analysis , Animals , Calibration , Indicators and Reagents , Liver/chemistry , Muscle, Skeletal/chemistry , Myocardium/chemistry , Pakistan , Reference Values , Spectrophotometry, Atomic , Surface-Active Agents/chemistry , TemperatureABSTRACT
The objective of this study was to evaluate the association between trace and toxic elements zinc (Zn), cadmium (Cd), nickel (Ni) and lead (Pb) in biological samples (scalp hair, blood and urine) of smoker and nonsmoker hypertensive patients (n=457), residents of Hyderabad, Pakistan. For the purpose of comparison, the biological samples of age-matched healthy controls were selected as referents. The concentrations of trace and toxic elements were measured by atomic absorption spectrophotometer prior to microwave-assisted acid digestion. The validity and accuracy of the methodology were checked using certified reference materials and by the conventional wet acid digestion method on the same certified reference materials and real samples. The recovery of all the studied elements was found to be in the range of 97.8-99.3% in certified reference materials. The results of this study showed that the mean values of Cd, Ni and Pb were significantly higher in scalp hair, blood and urine samples of both smoker and nonsmoker patients than in referents (P<0.001), whereas the concentration of Zn was lower in the scalp hair and blood, but higher in the urine samples of hypertensive patients. The deficiency of Zn and the high exposure of toxic metals as a result of tobacco smoking may be synergistic with risk factors associated with hypertension.
Subject(s)
Cadmium/analysis , Hypertension/metabolism , Lead/analysis , Nickel/analysis , Smoking/metabolism , Zinc/analysis , Adult , Cadmium/blood , Cadmium/urine , Female , Hair/chemistry , Humans , Lead/blood , Lead/urine , Male , Middle Aged , Nickel/blood , Nickel/urine , Zinc/blood , Zinc/urineABSTRACT
In situ forming polymeric formulations are drug delivery systems that are in sol form before administration in the body, but once administered, undergo gelation in situ, to form a gel. The formation of gels depends on factors like temperature modulation, pH change, presence of ions and ultra violet irradiation, from which the drug gets released in a sustained and controlled manner. Various polymers that are used for the formulation of in situ gels include gellan gum, alginic acid, xyloglucan, pectin, chitosan, poly(DL-lactic acid), poly(DL-lactide-co-glycolide) and poly-caprolactone. The choice of solvents like water, dimethylsulphoxide, N-methyl pyrrolidone, triacetin and 2-pyrrolidone for these formulations depends on the solubility of polymer used. Mainly in situ gels are administered by oral, ocular, rectal, vaginal, injectable and intraperitoneal routes. The in situ gel forming polymeric formulations offer several advantages like sustained and prolonged action in comparison to conventional drug delivery systems. The article presents a detailed review of these types of polymeric systems, their evaluation, advancements and their commercial formulations. From a manufacturing point of view, the production of such devices is less complex and thus lowers the investment and manufacturing cost.
ABSTRACT
The aim of present study was to develop a database of arsenic (As) in lake water, ground water, sediment, soil, vegetables, grain crops and fish to evaluate the potential human health risks posed by higher level of As, in south east part of Sindh, Pakistan during 2005-2007. The total concentration of As in various samples under study was determined by electrothermal atomic absorption spectrophotometry (ETAAS). The reliability and accuracy of technique was checked by different certified reference materials. The concentration of As in lake and ground water samples exceeded the WHO guideline values. The concentration of As in lake sediment and agricultural soil samples ranged between 11.3-55.8 and 8.7-46.2 mg/kg while acid soluble As (acetic acid 0.11 mol/L) was observed in the range of 1.48-3.54 and 0.34-3.78 mg/kg, respectively. It was observed that the leafy vegetables (spinach, coriander and peppermint) contain higher As levels (0.90-1.20 mg/kg) as compared to ground vegetables (0.048-0.25) and grain crops (0.248-0.367 mg/kg) on dried weight basis. The estimated daily intake of total As in the diet was 9.7-12.2 microg/kg body weight/day.
Subject(s)
Arsenic/analysis , Diet , Food Contamination/analysis , Fresh Water/chemistry , Geologic Sediments/chemistry , Animals , Edible Grain/chemistry , Fishes , Food Analysis , Humans , Pakistan , Vegetables/chemistryABSTRACT
The determination of trace and toxic elements in biological samples (blood, urine and scalp hair samples) of human beings is an important clinical test. The aim of our present study was to determine the concentration of arsenic (As), copper (Cu), cobalt (Co) and manganese (Mn), in biological samples of male production workers (PW) and quality control workers (QW) of steel mill, with aged 25-55 years, to assess the possible influence of environmental exposure. For comparison purpose, the same biological samples of unexposed healthy males of same age group were collected as control subjects. The determination of all elements in biological samples was carried out by electrothermal atomic absorption spectrometry, prior to microwave assisted acid digestion. The accuracy of the As, Cu, Co and Mn measurements was tested by simultaneously analyzing certified reference materials (CRMs) and for comparative purposes conventional wet acid digestion method was used on the same CRMs. No significant differences were observed between the analytical results and the certified values, using both methods (paired t-test at P > 0.05). The results indicate that concentrations of As, Cu, Co and Mn in all three biological samples of the exposed workers (QW and PW) were significantly higher than those of the controls. The possible correlation of these elements with the etiology of different physiological disorders is discussed. The results were also demonstrated the need of attention for improvements in workplace, ventilation and industrial hygiene practices.
Subject(s)
Arsenic/metabolism , Metallurgy , Metals, Heavy/metabolism , Occupational Exposure/adverse effects , Spectrophotometry, Atomic/methods , Adult , Arsenic/analysis , Cobalt/analysis , Cobalt/metabolism , Copper/analysis , Copper/metabolism , Hair/chemistry , Humans , Male , Manganese/analysis , Manganese/metabolism , Metals, Heavy/analysis , Middle Aged , SteelABSTRACT
We have studied the pharmacokinetics of methotrexate in patients with rheumatoid arthritis concurrently taking the most commonly used non-steroidal anti-inflammatory drugs (NSAIDs), aspirin, diclofenac, naproxen, indomethacin, and ibuprofen. The area under the curve, the total systemic clearance, the distribution volume, and the half-life of methotrexate in patients receiving concurrent NSAID therapy did not change significantly (at p < 0.05). Concurrent treatment with NSAIDs resulted in increased inter-patient variability of methotrexate concentration, possibly as a result of biochemical interactions; however, it does not appear clinically relevant. The data suggest that the NSAIDs do not significantly affect the disposition of methotrexate, contrary to some of the earlier reports.