Influence of school backpack load on plantar foot pressure during walking in 9-11 years old girls.

OBJECTIVE: This study aimed to assess the relative load of the midfoot and the metatarsals of both feet when schoolchildren walked with backpacks of different loads. METHODS: A group of 12 healthy girls (9.9 ± 0.8 years; 33.8 ± 6.7 kg, 1.40 ± 0.10 m) walked barefoot to assess plantar pressures during gait without load (L0%) and with a loaded backpack equal to 10% (L10%) and 20% (L20%) of their body weight. A Footscan® system (RSscan International, Belgium) was used to determine the contact area and relative pressure impulse in the midfoot and metatarsals on the dominant (DL) and non-dominant legs (NL). RESULTS: The effect of load was significant for the contact area of the midfoot for both NL (p = 0.013) and DL (p = 0.001). In the metatarsals, there was significantly greater relative impulse during L10% compared to L0% in the first (p = 0.041) and second (p = 0.050) metatarsals of the DL. Comparing the NL and DL showed significantly greater relative impulse on the DL in the fourth metatarsal during L10% (p = 0.023), greater contact area in the fifth metatarsal during L0% (p = 0.050), and greater impulse in the midfoot during L20% (p = 0.028) on the NL. CONCLUSIONS: The school backpack load influences relative plantar pressure distribution, especially in the midfoot. Further, our findings suggest greater propulsion of the DL and supporting function of the NL.

Quantitative Assessment of Upper Limb Movement in Post-Stroke Adults for Identification of Sensitive Measures in Reaching and Lifting Activities.

BACKGROUND: The assumption of this work is the achievement of objective results of the movement structure, which forms the basis for in-depth analysis and, consequently, for determining the upper limb movements that are most affected by stroke compared to healthy people. METHODS: An analysis of relevant and systematically identified features of upper limb movement in post-stroke adults is presented based on scalable hypothesis tests. The basic features were calculated using movements defined by the x, y, and z coordinates (i.e., 3D trajectory time series) and compared to the results of post-stroke patients with healthy controls of similar age. RESULTS: After automatic feature selection, out of the 1004 common features of upper limb movement, the most differentiated were the upper arm movements in reaching kinematics. In terms of movement type, movements in the frontal plane (shoulder abduction and adduction) were the most sensitive to changes. The largest number of discriminating features was determined on the basis of acceleration time series. CONCLUSIONS: In the 3D assessment of functional activities of the upper limb, the upper arm turned out to be the most differentiated body segment, especially during abduction and adduction movements. The results indicate a special need to pay attention to abduction and adduction movements to improve the activities of daily living of the upper limbs after a stroke.

Paracrine signalling between keratinocytes and SVF cells results in a new secreted cytokine profile during wound closure.

Stromal vascular fraction (SVF) cells, and the adipose-derived mesenchymal stem cells they contain, have shown enhanced wound healing in vitro and in vivo, yet their clinical application has been limited. In this regard, understanding the mechanisms that govern SVF-enhanced wound healing would improve their application in the clinic. Here, we show that the SVF cells and keratinocytes engage in a paracrine crosstalk during wound closure, which results in a new cytokine profile that is distinct from the cytokines regularly secreted by either cell type on their own. We identify 11 cytokines, 5 of which are not regularly secreted by the SVF cells, whose expressions are significantly increased during wound closure by the keratinocytes. This new cytokine profile could be used to accelerate wound closure and initiate re-epithelialization without the need to obtain the SVF cells from the patient.

A Robust and Standardized Approach to Quantify Wound Closure Using the Scratch Assay.

The scratch assay is an in vitro assay that allows for high-throughput quantification of wound closure by keratinocytes and fibroblasts with relative ease. However, this assay is amenable to experimental variables, which can result in false-positive and false-negative data, making the interpretation of such data difficult. Also, data variability decreases the sensitivity of the scratch assay. Here, we identify important sources of data variation in the scratch assay and provide rational mitigation strategies that enable robust and highly reproducible quantification of scratch width and area, and ultimately the scratch closure rates. By eliminating these sources of variability, the sensitivity of the scratch assay is enhanced, thereby allowing for identification of dependent variables with wide-ranging impacts on wound closure in a robust and standardized manner.

Force-Velocity Relationship in Cycling and Arm Cranking: A Comparison of Men and Women.

This study was aimed at comparing the force-velocity relationship during cycling and arm cranking in males and females. Thirty-two male and twenty-two female healthy volunteers performed a force-velocity test on a cycle ergometer and a cranking ergometer in a randomly selected order. The theoretical values of the maximum force at zero speed (F0) and the maximum velocity at zero braking force (v0) for the lower and upper limbs were determined, and the maximum anaerobic power (Pmax) was calculated from the individual force-velocity relationship. The Pmax and F0 of the upper limbs related to the lower limbs correspond to 78.2 ± 14.3% and 80.1 ± 17.3% in men and 65.5 ± 12.5% and 74.5 ± 6.5% in women, respectively. The theoretical maximum velocity v0 of the upper limbs to the lower limbs attained 129.1 ± 29.0% in men and 127.4 ± 26.4% in women. The results of the study can serve as reference data for the force-velocity characteristics of the upper and lower limbs of male and female athletes. The results can be used both in training and rehabilitation programs, where the starting point is the objectification of possible strength deficits in various areas of the force-velocity characteristic spectrum of the muscles of the upper and lower limbs.

A Review of Exercise as Medicine in Cardiovascular Disease: Pathology and Mechanism.

BACKGROUND: Physical inactivity and resultant lower energy expenditure contribute unequivocally to cardiovascular diseases, such as coronary artery disease and stroke, which are considered major causes of disability and mortality worldwide. AIM: The aim of the study was to investigate the influence of physical activity (PA) and exercise on different aspects of health - genetics, endothelium function, blood pressure, lipid concentrations, glucose intolerance, thrombosis, and self - satisfaction. Materials and. METHODS: In this article, we conducted a narrative review of the influence PA and exercise have on the cardiovascular system, risk factors of cardiovascular diseases, searching the online databases; Web of Science, PubMed and Google Scholar, and, subsequently, discuss possible mechanisms of this action. RESULTS AND DISCUSSION: Based on our narrative review of literature, discussed the effects of PA on telomere length, nitric oxide synthesis, thrombosis risk, blood pressure, serum glucose, cholesterol and triglycerides levels, and indicated possible mechanisms by which physical training may lead to improvement in chronic cardiovascular diseases. CONCLUSION: PA is effective for the improvement of exercise tolerance, lipid concentrations, blood pressure, it may also reduce the serum glucose level and risk of thrombosis, thus should be advocated concomitant to, or in some cases instead of, traditional drug-therapy.

Stochastic Resonance with Dynamic Compression Improves the Growth of Adult Chondrocytes in Agarose Gel Constructs.

Dynamic mechanical stimulation has been an effective method to improve the growth of tissue engineering cartilage constructs derived from immature cells. However, when more mature cell populations are used, results are often variable due to the differing responses of these cells to external stimuli. This can be especially detrimental in the case of mechanical loading. In previous studies, multi-modal mechanical stimulation in the form of stochastic resonance was shown to be effective at improving the growth of young bovine chondrocytes. Thus, the aim of this study was to investigate the short-term and long-term effects of stochastic resonance on two groups of bovine chondrocytes, adult (> 30 month) and juvenile (~ 18 months). While the juvenile cells outperformed the adult cells in terms of their anabolic response to loading, combined mechanical loading for both age groups resulted in greater matrix synthesis compared to compressive loading alone. In the adult cells, potential pathological tissue formation was evident with the presence of cell clustering. However, the presence of broad-band mechanical vibrations (alone or with compressive loading) appeared to mitigate this response and allow these cells to attain a growth response similar to the juvenile, unstimulated cells. Therefore, the use of stochastic resonance appears to show promise as a method to improve the formation and properties of tissue engineered cartilage constructs, irrespective of cell age.

Physical Activity and Alzheimer's Disease: A Narrative Review.

Although age is a dominant risk factor for Alzheimer's disease (AD), epidemiological studies have shown that physical activity may significantly decrease age-related risks for AD, and indeed mitigate the impact in existing diagnosis. The aim of this study was to perform a narrative review on the preventative, and mitigating, effects of physical activity on AD onset, including genetic factors, mechanism of action and physical activity typology. In this article, we conducted a narrative review of the influence physical activity and exercise have on AD, utilising key terms related to AD, physical activity, mechanism and prevention, searching the online databases; Web of Science, PubMed and Google Scholar, and, subsequently, discuss possible mechanisms of this action. On the basis of this review, it is evident that physical activity and exercise may be incorporated in AD, notwithstanding, a greater number of high-quality randomised controlled trials are needed, moreover, physical activity typology must be acutely considered, primarily due to a dearth of research on the efficacy of physical activity types other than aerobic.

Functional differences in upper limb movement after early and chronic stroke based on kinematic motion indicators.

AIMS: The main purpose of this study was to determine the changes in kinematic parameters of ischemic stroke affected upper limbs, during simple functional activity, to determine the most relevant changes. METHODS: The OptiTrack system was used for motion capture. To determine upper extremity function in Activities of Daily Living (ADL) tasks. During particular phases, the following matrices were chosen: mean and peak speed, normalized movement unit, normalized jerk and phase movement time. The chosen matrices represent the speed and smoothness profile of end-point data. The the arm-trunk compensation was also taken into consideration. Twenty stroke patients, in early (G1 from 1 to 3 months after stroke) and chronic stage (G2 from 6 months to 1 year), were studied. The large and small cylinder forward and back transporting phases were evaluated. RESULTS: The most significant differences between groups G1 and G2 were in mean and peak speed of the forward transport of the large and small cylinders for the paretic limb. Significant differences were also found for the smoothness (measured by movement unit, mean and peak speed and jerk) where the G2 group had a rougher motion. There were also differences in arm-trunk compensation in the frontal plane. CONCLUSION: The variables used in the study showed applicability in assessing kinematic parameters in both the early and chronic period after stroke.