ABSTRACT
Atrazine is one of the most prevalent herbicides that has been widely applied to agricultural lands in the U.S. Understanding the transport and accumulation of atrazine in the subsurface under future climate scenarios is essential for future agriculture and water management. Here, we predict atrazine transport and accumulation under an intensive corn production land based on 20 projected global climate model (GCM) realizations, while considering uncertainties of transport parameters. Our study predicted continuous groundwater table declination and atrazine mass accumulation on the study site. We show that atrazine mass accumulation in corn production areas is subject to total precipitation in the atrazine application season, whereas atrazine plume movement is controlled by the sequence of annual precipitation. Atrazine mass transport and accumulation are more sensitive to climate variation on the field sites with low sorption and atrazine degradation rate. Under the extreme condition, the atrazine plume can migrate as far as five meters from the ground surface in only three years. While annual mean precipitation in the Midwestern U.S. is projected to increase in the future, groundwater vulnerability to atrazine and associated water quality impacts may rise in the U.S. Corn Belt, especially in sites with low atrazine degradation and sorption.
Subject(s)
Atrazine , Groundwater , Herbicides , Agriculture , Midwestern United States , Zea maysABSTRACT
A Municipal Solid Waste Borehole Assessment (MBA) was developed to assess in situ geotechnical properties of municipal solid waste (MSW) during the boring of gas extraction well construction. A Large-Diameter Borehole Caliper (LDBC) was lowered into the borehole to measure the diameter and record the condition of the wall by time-lapse video photography. The results indicated that the borehole experienced significant radial compression with depth following completion. Radial compressions amounted to approximately 7.5% at 9.14 m, 10% at 21.3 m and 11% at 27.4 m below ground surface. The bulk modulus was estimated by using the captured volumetric strains and reported lateral earth coefficients, and the results showed that it increases with increasing depth. For MSW, the bulk modulus increased up to 13.4 MPa in a linear trend with depth. The unit weights of MSW were obtained using three diameter readings from LDBC, auger barrel outside diameter and outer cutting bit outside diameter. The results showed that the diameter based on outer cutting bit yielded realistic unit weights (5.08-9.68 kN m-3) due to unrealistic calculated saturations by other two assumed diameters. The borehole assessment with LDBC was shown to be an efficient and valuable means for characterising MSW and effectively designing gas extraction wells. The research provided a means to assess the waste mass with accuracy at great depths by directly observing and measuring borehole condition.
Subject(s)
Refuse Disposal , Solid Waste , Pressure , Refuse Disposal/methods , Solid Waste/analysis , Waste Disposal FacilitiesABSTRACT
Manure storage methods can affect the concentration and prevalence of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in cattle manure prior to land application. The objective of this study was to compare stockpiling and composting with respect to their effectiveness in reducing ARB and ARGs in beef cattle manure in a field-scale study. Field experiments were conducted in different seasons with different bulking agents for composting. For both the winter-spring cycle and the summer-fall cycle, ARB concentrations declined below the limit of quantification rapidly in both composting piles and stockpiles; however, ARB prevalence was significantly greater in the composting piles than in the stockpiles. This was likely due to the introduction of ARB from bulking agents. There was no significant change in ARG concentrations between initial and final concentrations for either manure storage treatment during the winter-spring cycle, but a significant reduction of the ARGs erm(B), tet(O), and tet(Q) over time was observed for both the composting pile and stockpile during the summer-fall cycle. Results from this study suggest that (i) bulking agent may be an important source of ARB and ARGs for composting; (ii) during cold months, the heterogeneity of the temperature profile in composting piles could result in poor ARG reduction; and (iii) during warm months, both stockpiling and composting can be effective in reducing ARG abundance. IMPORTANCE Proper treatment of manure is essential to reduce the spread of antibiotic resistance and protect human health. Stockpiling and composting are two manure storage methods which can reduce antibiotic-resistant bacteria and resistance genes, although few field-scale studies have examined the relative efficiency of each method. This study examined the ability of both methods in both winter-spring and summer-fall cycles, while also accounting for heterogeneity within field-scale manure piles. This study determined that bulking agents used in composting could contribute antibiotic-resistant bacteria and resistance genes. Additionally, seasonal variation could hinder the efficacy of composting in colder months due to heterogeneity in temperature within the pile; however, in warmer months, either method of manure storage could be effective in reducing the spread of antibiotic resistance.
Subject(s)
Bacteria/drug effects , Bacterial Proteins/genetics , Composting/methods , Drug Resistance, Bacterial , Manure/microbiology , Animals , Anti-Bacterial Agents/pharmacology , Bacteria/genetics , Bacteria/isolation & purification , Bacteria/metabolism , Bacterial Proteins/metabolism , Biodegradation, Environmental , Cattle , Soil/chemistryABSTRACT
To meet global health and sanitation goals, there is a continued need for sustainable wastewater treatment alternatives that require minimal energy and investment. Vermifiltration, a technology gaining relevance in Africa and Asia, may be an alternative to traditional wastewater treatment systems due to its cost-effectiveness, ease of application and maintenance, and sustainability. However, nitrogen removal in vermifiltration is not well understood since most of the prior research focuses on organics removal. Thus, a state of the art review is necessary to separately focus on the mechanisms associated with nitrogen removal in vermifiltration, along with the factors affecting nitrogen removal. For the first time, this review attempts to present the types of vermifilter based on their flow pattern. The review further discusses the current status of the application of vermifiltration, along with the benefits and limitations associated with the adoption of this technology. It also explores possible strategies that could be adopted to maximize the nitrogen removal potential of vermifilters as optimizing nitrogen removal is critical for improving the performance of vermifiltration based treatment systems.
Subject(s)
Nitrogen , Oligochaeta , Africa , Animals , Asia , Denitrification , Waste Disposal, Fluid , WastewaterABSTRACT
The environmental spread of antibiotics and antibiotic resistance genes (ARGs) from the land application of livestock wastes can be a potential public health threat. The objective of this study was to assess the effects of setback distance, which determines how close manure may be applied in relation to surface water, on the transport of antibiotics and ARGs in runoff and soil following land application of swine manure slurry. Rainfall simulation tests were conducted on field plots covered with wheat residues, each of which contained an upslope manure region where slurry was applied and an adjacent downslope setback region that did not receive slurry. Results show that all three antibiotics (chlortetracycline, lincomycin, and tiamulin) and seven out of the ten genes tested (erm(B), erm(C), intI1, tet(O), tet(Q), tet(X), and the 16S rRNA gene) decreased significantly in runoff with increased setback distance. Only blaTEM, chlortetracycline, and tiamulin decreased significantly in surface soil with increased setback distance, while the other analytes did not exhibit statistically significant trends. By using linear regression models with field data, we estimate that a setback distance between 34-67 m may allow manure-borne antibiotics and ARGs in runoff to reach background levels under the experimental conditions tested.
Subject(s)
Manure , Soil , Animals , Anti-Bacterial Agents/pharmacology , Drug Resistance, Microbial/drug effects , Genes, Bacterial/drug effects , RNA, Ribosomal, 16S , Soil Microbiology , SwineABSTRACT
Chronic wasting disease (CWD) is an emerging prion disease in North America. Recent identification of CWD in wild cervids from Norway raises the concern of the spread of CWD in Europe. CWD infectivity can enter the environment through live animal excreta and carcasses where it can bind to soil. Well-characterized hamster prion strains and CWD field isolates in unadsorbed or soil-adsorbed forms that were either hydrated or dehydrated were subjected to repeated rounds of freezing and thawing. We found that 500 cycles of repeated freezing and thawing of hydrated samples significantly decreased the abundance of PrPSc and reduced protein misfolding cyclic amplification (PMCA) seeding activity that could be rescued by binding to soil. Importantly, dehydration prior to freezing and thawing treatment largely protected PrPSc from degradation, and the samples maintained PMCA seeding activity. We hypothesize that redistribution of water molecules during the freezing and thawing process alters the stability of PrPSc aggregates. Overall, these results have significant implications for the assessment of prion persistence in the environment.IMPORTANCE Prions excreted into the environment by infected animals, such as elk and deer infected with chronic wasting disease, persist for years and thus facilitate horizontal transmission of the disease. Understanding the fate of prions in the environment is essential to control prion disease transmission. The significance of our study is that it provides information on the possibility of prion degradation and inactivation under natural weathering processes. This information is significant for remediation of prion-contaminated environments and development of prion disease control strategies.
Subject(s)
Desiccation , Freezing , PrPSc Proteins/chemistry , Animals , Mesocricetus , Protein Stability , Wasting Disease, Chronic/metabolismABSTRACT
Citizen science is a research tool capable of addressing major environmental challenges, including contamination of water resources by agrichemicals, such as nutrients and pesticides. The objectives of this study were (1) to identify the proportion of accurate observations by citizen scientists using rapid assessment water quality tools, and (2) to characterize how a user's prior experience with water quality tools was associated with the accuracy of citizen scientists. To achieve these objectives, we conducted group testing with over 136 citizen scientists and compared their results from water quality testing of water samples to results obtained using laboratory analytical methods. Following brief training, we observed that accuracy of reported results varies based on the user's experience level where experienced and expert users shared consistent and reliable measurements. Where erroneous measures were reported, citizen scientists tend to overestimate contaminant concentrations when using colorimetric water quality tools. Additionally, we identified differences in accuracy related to the types of water quality assessment tools used by citizen scientists from each experience group. This study demonstrates the importance of evaluating participant background experience in designing citizen science campaigns.
Subject(s)
Pesticides , Water QualityABSTRACT
Prions enter the environment from infected hosts, bind to a wide range of soil and soil minerals, and remain highly infectious. Environmental sources of prions almost certainly contribute to the transmission of chronic wasting disease in cervids and scrapie in sheep and goats. While much is known about the introduction of prions into the environment and their interaction with soil, relatively little is known about prion degradation and inactivation by natural environmental processes. In this study, we examined the effect of repeated cycles of drying and wetting on prion fitness and determined that 10 cycles of repeated drying and wetting could reduce PrP(Sc) abundance, PMCA amplification efficiency and extend the incubation period of disease. Importantly, prions bound to soil were more susceptible to inactivation by repeated cycles of drying and wetting compared to unbound prions, a result which may be due to conformational changes in soil-bound PrP(Sc) or consolidation of the bonding between PrP(Sc) and soil. This novel finding demonstrates that naturally-occurring environmental process can degrade prions.
Subject(s)
PrPSc Proteins/metabolism , Prion Diseases/transmission , Soil , Adsorption , Animals , Blotting, Western , Cricetinae , Mesocricetus , Soil/chemistryABSTRACT
Stereoisomers of estradiol (E2) or trenbolone (TB) can occur together in the environment receiving human or livestock wastes. However, the effect of their co-occurrence on persistence has not been well elucidated. A sandy and a silt loam sediment were used to establish microcosms with α- and ß-isomers of E2 or TB spiked individually and together. Sediments were sampled periodically and analyzed for E2 and TB isomers and their transformation products using derivatization gas chromatography-mass spectrometry. Results showed that stereoselective degradation was significant for E2 in both sediments and TB in the sandy sediment with ß-isomers decaying more rapidly than α-isomers. In the sandy sediment containing limited natural organic carbon and nutrients, co-occurrence of both isomers of either E2 or TB decreased the dissipation rates. In the silt loam sediment with abundant organic matter and nutrients, the decay rates of both isomers were not changed in the presence of the other isomer. Estrone (E1) and trendione (TD) were detected as primary metabolites of E2 and TB isomers, respectively. The formation and decay profiles of E1 were similar in both sediments with 92-100% of E2 transformed to E1. The TD profiles were different across sediments with â¼100% of TB transformed to TD except in the sandy sediment where 51-60% of 17α-TB was converted to TD. These results indicate that the transformation processes of steroid hormone are stereoselective in sediment and co-occurrence of stereoisomers can prolong steroid persistence and thus pose greater environmental risk.
Subject(s)
Estradiol , Trenbolone Acetate , Estrone/metabolism , Gas Chromatography-Mass Spectrometry , Geologic Sediments , IsomerismABSTRACT
The environmental fate and bioavailability of progesterone, a steroid hormone known to cause endocrine-disrupting effects in aquatic organisms, is of growing concern due to its occurrence in the environment in water and sediment influenced by wastewater treatment plant and paper mill effluents, as well as livestock production. The objective of this study was to evaluate the fate of progesterone in two natural sediments and the corresponding alteration of gene expression in three steroid-responsive genes; vitellogenin, androgen receptor and estrogen receptor alpha. When exposed to progesterone-spiked sand, fathead minnows (Pimephales promelas) exhibited significant reductions in the expression of vitellogenin and androgen receptor expression. In contrast, fish exposed to progesterone associated with the silty loam sediment did not show a biological response at 7 days and only realized a significant reduction in vitellogenin. In both sediments, progesterone degradation resulted in the production of androgens including androsteinedione, testosterone, and androstadienedione, as well as the antiestrogen, testolactone. Differences in compound fate resulted in organism exposure to different suites of metabolites either in water or associated with the sediment. Results from this study suggest that environmental progestagens will lead to defeminization at environmentally relevant concentrations, and that exposure is influenced by sediment properties.
Subject(s)
Aquatic Organisms/metabolism , Cyprinidae/metabolism , Environmental Monitoring , Geologic Sediments/chemistry , Progesterone/analysis , Androgens/analysis , Animals , Biological Availability , Estrogen Receptor Modulators/analysis , Female , Liver/metabolism , Progesterone/chemistry , Receptors, Androgen/metabolism , Testosterone/analysis , Vitellogenins/metabolism , Water Pollutants, Chemical/analysisABSTRACT
We propose a simple causal model depicting relationships involved in dissemination of antibiotics and antibiotic resistance in agroecosystems and potential effects on human health, functioning of natural ecosystems, and agricultural productivity. Available evidence for each causal link is briefly summarized, and key knowledge gaps are highlighted. A lack of quantitative estimates of human exposure to environmental bacteria, in general, and antibiotic-resistant bacteria, specifically, is a significant data gap hindering the assessment of effects on human health. The contribution of horizontal gene transfer to resistance in the environment and conditions that might foster the horizontal transfer of antibiotic resistance genes into human pathogens also need further research. Existing research has focused heavily on human health effects, with relatively little known about the effects of antibiotics and antibiotic resistance on natural and agricultural ecosystems. The proposed causal model is used to elucidate gaps in knowledge that must be addressed by the research community and may provide a useful starting point for the design and analysis of future research efforts.
Subject(s)
Anti-Bacterial Agents , Drug Resistance, Microbial , Ecosystem , Agriculture , Bacteria , HumansABSTRACT
A total of 59 papers published in 2015 were reviewed ranging from detailed descriptions of analytical methods, to fate and occurrence studies, to ecological effects and sampling techniques for a wide variety of emerging contaminants likely to occur in agricultural environments. New methods and studies on veterinary pharmaceuticals, steroids, antibiotic resistance genes in agricultural environments continue to expand our knowledge base on the occurrence and potential impacts of these compounds. This review is divided into the following sections: Introduction, Analytical Methods, Steroid Hormones, Pharmaceutical Contaminants, Transformation Products, and "Antibiotic Resistance, Drugs, Bugs and Genes".
Subject(s)
Agriculture , Environmental Monitoring , Environmental Pollutants , Ecology , Pharmaceutical PreparationsABSTRACT
Precipitation induced runoff is an important pathway for agrichemicals to enter surface water systems and expose aquatic organisms to endocrine-disrupting compounds such as pesticides and steroid hormones. The objectives of this study were to investigate the distribution of agrichemicals between dissolved and sediment-bound phases during spring pulses of agrichemicals and to evaluate the role of suspended sediment in agrichemical bioavailability to aquatic organisms. To accomplish these objectives, suspended sediment and water samples were collected every 3 days from a field site along the Elkhorn River, located at the downstream end of a heavily agricultural watershed, and were screened for 21 pesticides and 21 steroids. Adult female fathead minnows (Pimephales promelas) were exposed in field mesocosms to river water containing varying sediment loads. Changes in organism hepatic gene expression of two estrogen-responsive genes, vitellogenin (VTG) and estrogen receptor alpha (ERα), as well as the androgen receptor (AR) were analyzed during periods of both low and high river discharge. Trends in agrichemical concentrations of both the dissolved and sediment phases as a function of time show that, while sediment may act as both a source and a sink for agrichemicals following precipitation events, the overall driver for molecular defeminization in this system is direct exposure to the sediment-associated compounds. This study suggests that endocrine disrupting effects observed in organisms in turbid water could be attributed to direct exposure of contaminated sediment.
Subject(s)
Agrochemicals/analysis , Cyprinidae/metabolism , Environmental Monitoring , Geologic Sediments/chemistry , Water Pollutants, Chemical/analysis , Animals , Biological Availability , Cyprinidae/genetics , Endocrine Disruptors/analysis , Female , Gene Expression Regulation , Nebraska , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rivers/chemistryABSTRACT
Pharmaceutical fate in surface water depends on a combination of physical and chemical processes, but currently, little information is available on cumulative dissipation rates in effluent-dominated receiving waters. In this study, dissipation rates were calculated for pharmaceutical compounds in two streams receiving municipal wastewater effluents using results from passive samplers. Seventeen pharmaceuticals were detected at the two sampling locations, and first-order dissipation rates were found to range between 0.03 and -0.02 h, indicating that some compounds were conserved, while others rapidly dissipated over several kilometers downstream of the source.
ABSTRACT
Agricultural runoff from areas receiving livestock manure can potentially contaminate surface water with antimicrobials and antimicrobial resistance genes (ARGs). The objective of this study was to investigate the effectiveness of narrow grass hedges (NGHs) on reducing the transport of antimicrobials and ARGs in runoff after land application of swine manure slurry. Plot-scale rainfall simulation tests were conducted on 0.75 m by 4.0 m plots designed to test three treatment factors: manure amendment (control plots receiving no manure vs. amended plots receiving manure based on 3 times N requirement), NGH (plots with a NGH vs. plots without a NGH), and rainfall events (days 1-3). Runoff generated during three 30-min simulated rainfall events was sampled and analyzed for antimicrobials and ARGs. Manure amendment was responsible for the presence of antimicrobial tylosin ( < 0.0001) and tylosin resistance gene (B) ( < 0.0001) in runoff. Narrow grass hedges proved to be effective in reducing tylosin ( < 0.0001) and (B) ( < 0.0347) in runoff. Manure amendment was responsible for the introduction of tylosin ( < 0.0482) and (B) ( = 0.0128) into the soil; however, it had no significant impact on the abundance of the 16S rRNA gene in soil. Results from this study suggest that NGHs could be a best management practice to control the transport of antimicrobials and ARGs in agricultural runoff.
ABSTRACT
UNLABELLED: The objectives of this study were to quantify real-world activity, fuel use, and emissions for heavy duty diesel roll-off refuse trucks; evaluate the contribution of duty cycles and emissions controls to variability in cycle average fuel use and emission rates; quantify the effect of vehicle weight on fuel use and emission rates; and compare empirical cycle average emission rates with the U.S. Environmental Protection Agency's MOVES emission factor model predictions. Measurements were made at 1 Hz on six trucks of model years 2005 to 2012, using onboard systems. The trucks traveled 870 miles, had an average speed of 16 mph, and collected 165 tons of trash. The average fuel economy was 4.4 mpg, which is approximately twice previously reported values for residential trash collection trucks. On average, 50% of time is spent idling and about 58% of emissions occur in urban areas. Newer trucks with selective catalytic reduction and diesel particulate filter had NOx and PM cycle average emission rates that were 80% lower and 95% lower, respectively, compared to older trucks without. On average, the combined can and trash weight was about 55% of chassis weight. The marginal effect of vehicle weight on fuel use and emissions is highest at low loads and decreases as load increases. Among 36 cycle average rates (6 trucks×6 cycles), MOVES-predicted values and estimates based on real-world data have similar relative trends. MOVES-predicted CO2 emissions are similar to those of the real world, while NOx and PM emissions are, on average, 43% lower and 300% higher, respectively. The real-world data presented here can be used to estimate benefits of replacing old trucks with new trucks. Further, the data can be used to improve emission inventories and model predictions. IMPLICATIONS: In-use measurements of the real-world activity, fuel use, and emissions of heavy-duty diesel roll-off refuse trucks can be used to improve the accuracy of predictive models, such as MOVES, and emissions inventories. Further, the activity data from this study can be used to generate more representative duty cycles for more accurate chassis dynamometer testing. Comparisons of old and new model year diesel trucks are useful in analyzing the effect of fleet turnover. The analysis of effect of haul weight on fuel use can be used by fleet managers to optimize operations to reduce fuel cost.
Subject(s)
Air Pollutants/chemistry , Gasoline , Motor Vehicles , Refuse Disposal , Vehicle Emissions , Environmental Monitoring/methods , Time FactorsABSTRACT
A total of 43 papers published in 2014 were reviewed ranging from detailed descriptions of analytical methods, to fate and occurrence studies, to measuring and predicting biological effects for a wide variety of emerging contaminants likely to occur in agricultural environments. New methods and studies on veterinary pharmaceuticals, natural and synthetics steroids, and antibiotic resistance genes in agricultural environments continue to expand our knowledge base on the occurrence and potential impacts of these compounds. This review is divided into the following sections: Introduction, Analytical Methods, Occurrence and Fate, Antibiotic Resistance Genes, and Risk Assessment.
ABSTRACT
The use of wetlands as a treatment approach for nitrogen in runoff is a common practice in agroecosystems. However, nitrate is not the sole constituent present in agricultural runoff and other biologically active contaminants have the potential to affect nitrate removal efficiency. In this study, the impacts of the combined effects of four common veterinary antibiotics (chlortetracycline, sulfamethazine, lincomycin, monensin) on nitrate-N treatment efficiency in saturated sediments and wetlands were evaluated in a coupled microcosm/mesocosm scale experiment. Veterinary antibiotics were hypothesized to significantly impact nitrogen speciation (e.g., nitrate and ammonium) and nitrogen uptake and transformation processes (e.g., plant uptake and denitrification) within the wetland ecosystems. To test this hypothesis, the coupled study had three objectives: 1. assess veterinary antibiotic impact on nitrogen cycle processes in wetland sediments using microcosm incubations, 2. measure nitrate-N reduction in water of floating treatment wetland systems over time following the introduction of veterinary antibiotic residues, and 3. identify the fate of veterinary antibiotics in floating treatment wetlands using mesocosms. Microcosms containing added mixtures of the veterinary antibiotics had little to no effect at lower concentrations but stimulated denitrification potential rates at higher concentrations. Based on observed changes in the nitrogen loss in the microcosm experiments, floating treatment wetland mesocosms were enriched with 1000 µg L-1 of the antibiotic mixture. Rates of nitrate-N loss observed in mesocosms with the veterinary antibiotic enrichment were consistent with the microcosm experiments in that denitrification was not inhibited, even at the high dosage. In the mesocosm experiments, average nitrate-N removal rates were not found to be impacted by the veterinary antibiotics. Further, veterinary antibiotics were primarily found in the roots of the floating treatment wetland biomass, accumulating approximately 190 mg m-2 of the antibiotic mixture. These findings provide new insight into the impact that veterinary antibiotic mixtures may have on nutrient management strategies for large-scale agricultural operations and the potential for veterinary antibiotic removal in these wetlands.
ABSTRACT
Due to the use of antimicrobials in livestock production, residual antimicrobials and antimicrobial resistance genes (ARGs) could enter the environment following the land application of animal wastes and could further contaminate surface and groundwater. The objective of this study was to determine the effect of various manure land application methods on the fate and transport of antimicrobials and ARGs in soil and runoff following land application of swine manure slurry. Swine manure slurries were obtained from facilities housing pigs that were fed chlortetracyline, tylosin or bacitracin and were land applied via broadcast, incorporation, and injection methods. Three rainfall simulation tests were then performed on amended and control plots. Results show that land application methods had no statistically significant effect on the aqueous concentrations of antimicrobials in runoff. However, among the three application methods tested broadcast resulted in the highest total mass loading of antimicrobials in runoff from the three rainfall simulation tests. The aqueous concentrations of chlortetracyline and tylosin in runoff decreased in consecutive rainfall events, although the trend was only statistically significant for tylosin. For ARGs, broadcast resulted in significantly higher erm genes in runoff than did incorporation and injection methods. In soil, the effects of land application methods on the fate of antimicrobials in top soil were compound specific. No clear trend was observed in the ARG levels in soil, likely because different host cells may respond differently to the soil environments created by various land application methods.