Biosynthesis of selenium nanoparticles using cell-free extract of Xenorhabdus cabanillasii GU480990 and their potential mosquito larvicidal properties against yellow fever mosquito Aedes aegypti.

Nanomaterials are successful due to their numerous applications in various domains such as cancer treatment, environmental applications, drug and gene delivery. Selenium is a metalloid element with broad biological activities and low toxicity especially at the nanoscale. Several studies have shown that nanoparticles synthesized from microbial and plant extracts are effective against important pests and pathogens. This study describes the bio fabrication of selenium nanoparticles using cell free extract of Xenorhabdus cabanillasii (XC-SeNPs) and assessed their mosquito larvicidal properties. Crystallographic structure and size of XC-SeNPs were determined with UV-a spectrophotometer, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD), Energy-dispersive X-ray spectroscopy (EDAX), Zeta potential and Transmission electron microscopy (TEM). The significant surface plasmon resonance at 275 nm indicated the synthesis of XC-SeNPs from the pure cell-free extract of X. cabanillasii. The XRD result exhibits the crystalline nature of XC-SeNPs. The Zeta potential analysis confirmed that the surface charge of XC-SeNPs was -24.17 mV. TEM analysis revealed that synthesized XC-SeNPs were monodispersed, spherically shaped, and sized about 80-200 nm range. In addition, the larvicidal potentials of the bio-fabricated XC-SeNPs were assessed against the 4th-instar Ae. aegypti. XC-SeNPs displayed a dose-dependent larvicidal effect; the larval mortality was 13.3 % at the minimum evaluated concentration and increased to 72 % at higher dose treatments. The LC50 and LC90 concentration of XC-SeNPs against mosquito larvae were 79.4 and 722.4 ppm, respectively.

Metabolic engineering of Escherichia coli for high-level production of violaxanthin.

BACKGROUND: Xanthophylls are a large class of carotenoids that are found in a variety of organisms and play particularly important roles in the light-harvesting and photoprotection processes of plants and algae. Violaxanthin is an important plant-derived xanthophyll with wide potential applications in medicines, foods, and cosmetics because of its antioxidant activity and bright yellow color. To date, however, violaxanthins have not been produced using metabolically engineered microbes on a commercial scale. Metabolic engineering for microbial production of violaxanthin is hindered by inefficient synthesis pathway in the heterologous host. We systematically optimized the carotenoid chassis and improved the functional expression of key enzymes of violaxanthin biosynthesis in Escherichia coli. RESULTS: Co-overexpression of crtY (encoding lycopene ß-cyclase), crtZ (encoding ß-carotene 3-hydroxylase), and ZEP (encoding zeaxanthin epoxidase) had a notable impact on their functions, resulting in the accumulation of intermediate products, specifically lycopene and ß-carotene. A chassis strain that did not accumulate the intermediate was optimized by several approaches. A promoter library was used to optimize the expression of crtY and crtZ. The resulting strain DZ12 produced zeaxanthin without intermediates. The expression of ZEP was further systematically optimized by using DZ12 as the chassis host. By using a low copy number plasmid and a modified dithiol/disulfide system, and by co-expressing a full electron transport chain, we generated a strain producing violaxanthin at about 25.28 ± 3.94 mg/g dry cell weight with decreased byproduct accumulation. CONCLUSION: We developed an efficient metabolically engineered Escherichia coli strain capable of producing a large amount of violaxanthin. This is the first report of a metabolically engineered microbial platform that could be used for the commercial production of violaxanthin.

Entanglement distribution over a 96-km-long submarine optical fiber.

Quantum entanglement is one of the most extraordinary effects in quantum physics, with many applications in the emerging field of quantum information science. In particular, it provides the foundation for quantum key distribution (QKD), which promises a conceptual leap in information security. Entanglement-based QKD holds great promise for future applications owing to the possibility of device-independent security and the potential of establishing global-scale quantum repeater networks. While other approaches to QKD have already reached the level of maturity required for operation in absence of typical laboratory infrastructure, comparable field demonstrations of entanglement-based QKD have not been performed so far. Here, we report on the successful distribution of polarization-entangled photon pairs between Malta and Sicily over 96 km of submarine optical telecommunications fiber. We observe around 257 photon pairs per second, with a polarization visibility above 90%. Our results show that QKD based on polarization entanglement is now indeed viable in long-distance fiber links. This field demonstration marks the longest-distance distribution of entanglement in a deployed telecommunications network and demonstrates an international submarine quantum communication channel. This opens up myriad possibilities for future experiments and technological applications using existing infrastructure.

Hypoxia-induced PINK1/Parkin-mediated mitophagy promotes pulmonary vascular remodeling.

Pulmonary vascular remodeling (PVR) is not only the main pathophysiological feature of Pulmonary Artery Hypertension (PAH) but also the main reason for the progressive aggravation of PAH. Its central link is the excessive proliferation of pulmonary artery smooth muscle cells (PASMCs), which leads to the imbalance of proliferation/apoptosis, leads to the formation of PAH. At present, we found that hypoxia can up-regulate the expression of mitophagy protein PINK1/Parkin, induce the proliferation of PASMCs, and inhibit apoptosis. Knocking down PINK1-/- and/or Parkin-/-, found that the proliferation of PASMCs was significantly inhibited compared with that of PINK1/Parkin, while the proliferation of cells under PINK1-/- Parkin-/- was significantly lower than that of PINK1-/- Parkin+/+or PINK1+/+ Parkin-/-. These results suggest that hypoxia can activate the PINK1/Parkin-mediated mitophagy pathway, induce the excessive proliferation of PASMCs, eventually lead to PVR, leading to HPH. Our team is further exploring which substances in HPH can induce mitotic response, which molecules specifically mediate the activation of mitotic pathways, and what role they play in the occurrence and development of HPH disease.

Dietary selenium enhances the growth and anti-oxidant capacity of juvenile blunt snout bream (Megalobrama amblycephala).

Sodium selenite was added to basal diet at five levels (0.10, 0.42, 0.67, 1.06 and 1.46 mg Se/kg) and fed fish for 8 weeks. The dietary selenium requirement of juvenile blunt snout bream (Megalobrama amblycephala) was quantified. Dietaryseleniums at 0.67-1.06 mg Se/kg improved weight gain rate, specific growth rate and feed efficiency. The optimal amount was 0.96 mg/kg, for which the specific growth rate was 1.798%/day and the weight gain rate was 173.852% (p < 0.05). Se deposition in muscle was increased (p < 0.05) at ≥0.67 mg/kg, but moisture, protein, lipid and ash content were not affected. Physiological status and lipid metabolism were improved by 1.06-1.46 mg/kg dietary selenium based on total protein and albumin in plasma, and total cholesterol and triglycerides (p < 0.05). Activities of hepatic anti-oxidant enzymes catalase, total superoxide dismutase, glutathione peroxidase and reduced glutathione were enhanced at Se1.06 (p < 0.05). However, malondialdehyde content was lowered at Se1.06 (p < 0.05). Expression of anti-inflammatory cytokines, nuclear factor erythroid 2-related factor 2 (Nrf2) and kelch-like ECH-associated protein 1 (Keap-1) in liver were elevated at Se1.06 (p < 0.05), as were mRNA levels of glutathione peroxidase, copper zinc superoxide dismutase and catalase. Expression of pro-inflammatory cytokines, interleukin 8, tumour necrosis factor-α and transforming growth factor-ß were inhibited at 0.67-1.46 mg/kg (p < 0.05). In general, 0.96 mg/kg was optimal, and optimal selenium enhanced antioxidant stress tolerance and anti-inflammatory ability.

[Research progress on chemical constituents and pharmacological activities of Hovenia].

The medicinal parts of Hovenia include the branches, leaves, seeds and fruits with inflorescence axis. This plant has been used with a long history as traditional herbal medicine for the treatment of alcoholism and liver protection. Modern pharmacological research shows that the crude extracts or pure compounds from Hovenia have anti-alcoholism, hepatoprotective, anti-hyperuricemia, anti-viral and anti-tumor activities, etc. Previous phytochemical studies have indicated that flavonoids, triterpenoid saponins, phenylpropanoids and polysaccharides are the major constituents in this plant. In this paper, we systematically summarized and analyzed the chemical constituents and pharmacological activities of Hovenia, providing the scientific evidences for the rational use and comprehensive development of Hovenia.

Cytotoxic effects, inflammatory response and apoptosis induction of cyclophosphamide in the peripheral blood leukocyte of blunt snout bream (Megalobrama amblycephala).

The present study was aimed to evaluate the effects of the cyclophosphamide (CY) exposure (Control, 0.032, 0.32, 1.0, 1.6 and 3.2 mg/mL) on the damage in the peripheral blood leukocytes of blunt snout bream for 24 h, which including cell viability, apoptosis, lactate dehydrogenase (LDH) release, mitochondrial membrane potential (Δѱm), ROS, antioxidant enzyme activity and the relative mRNA levels of apoptosis. Results showed that cell viability and Δѱm effects of CY were greatly reduced, and occurred in a dose-dependent manner. CY exposure (0.32-3.2 mg/mL) significantly increased the LDH release and induced apoptosis accompanied by ΔΨm disruption and ROS generation compared to the control. The cellular ROS was significantly increased with increase of CY level from 0.032 mg/mL to 1 mg/mL and the plateau occurred at 0.32 mg/mL. Additionally CY exposure led to oxidative stress as evidenced by significantly the decrease of SOD and CAT and increase of MDA concentration after treating cells with 3.2 mg/mL of CY. Besides, the relative mRNA levels of caspase-3 in the dose of 0.032, 0.32 mg/mL CY, caspase-9 and interleukins-1ß (IL-1ß) in the dose of 0.32 mg/mL CY, tumor necrosis factor-alpha (TNF-α) in the dose of 0.032 mg/mL CY significantly higher than that of the control. In conclusion, 0.32-3.2 mg/mL CY could lead to cytotoxic effect, inflammatory response and induce the apoptosis of the peripheral blood leukocyte of Megalobrama amblycephala.

Effects of Moringa oleifera leaf extract on growth performance, physiological and immune response, and related immune gene expression of Macrobrachium rosenbergii with Vibrio anguillarum and ammonia stress.

In order to study the effects of Moringa oleifera leaf extract on Macrobrachium rosenbergii under high ammonia exposure, freshwater prawns were randomly divided into five groups: a control group was fed with basal diet, and four treatment groups fed with basal diet supplemented with 0.25%, 0.5% and 1.0% M. oleifera leaf extract and 0.025% Enrofloxacin for 60 days, respectively. Then, freshwater prawns were exposed to high ammonia stress for 72 h and Vibro anguillarum infection. The growth, antioxidant capabilities, related immune genes as well as resistance to infection by V. anguillarum were determined. The results showed that compared with the control group, the weight gain, specific growth rate and protein efficiency rate, haemolymph catalase (CAT), superoxide dismutase (SOD) and inducible nitric oxide synthase (iNOS) increased while feed conversion ratio, haemolymph aspartate aminotransferase, alanine aminotransferase, nitrogen oxide (NO), hepatopancreas heat shock proteins (HSP70), immune deficiency (IMD) expression levels decreased in the group of 0.5% M. oleifera leaf extract before the stress. After ammonia stress, the group of 0.5% M. oleifera leaf extract also could improve the haemolymph SOD, glutathione peroxidase, NO, iNOS, hepatopancreas HSP70 expression levels and reduce haemolymph CAT, hepatopancreas peroxiredoxin 5 and NF kappa B inhibitor alpha expression level compared with the control group. The rate of mortality of the prawns challenged with V. anguillarum was lower in the supplemented groups in comparison with the control group with the lowest being in the group of 0.5% M. oleifera leaf extract. Antioxidant activities as well as biochemical parameters in the enrofloxacin group (0.025%E) were not significantly enhanced both pre and post challenge in comparison with the M. oleifera leaf extract groups, showing the superiority of the natural herb over the synthetic antibiotic. In summary, this study suggested that at an inclusion rate of 0.5%, M. oleifera leaf extract could increase the growth performance, even has positive effects on physiological and immune function and prevents high ammonia stress in the Freshwater prawn, M.rosenbergii.

Emerging Pollutants - Part II: Treatment.

Emerging contaminants are considered as some substances of actual or potential threat to human health or environment, which include pharmaceutical, personal care products, endocrine disruptors, nanoparticles, antibiotic resistance genes and chemicals used in packaging products, etc. How to treat these emerging pollutants in water bodies with green, high efficiency and low-cost has become the focus of attention. The purpose of this review is to summarize published researches on emerging pollutants treatment in 2017.

Emerging Pollutants - Part II: Treatment.

Emerging contaminants are considered as some substances of actual or potential threat to human health or environment, which include endocrine disruptors, pharmaceutical, personal care products, nanoparticles, antibiotic resistance genes and chemicals used in packaging products, etc. The disposal and treatment of emerging contaminants has become a key problem in the field of water pollution control. The purpose of this review is to summarize published researches on emerging pollutants treatment in 2015.

Theoretical and experimental research on the self-assembled system of molecularly imprinted polymers formed by salbutamol and methacrylic acid.

The quantum chemical method was applied for screening functional monomers in the rational design of salbutamol-imprinted polymers. Salbutamol was the template molecule, and methacrylic acid was the single functional monomer. The LC-WPBE/6-31G(d,p) method was used to investigate the geometry optimization, active sites, natural bond orbital charges, binding energies of the imprinted molecule, and solvation energy. The mechanism of action between salbutamol and methacrylic acid was also discussed. The theoretical results show that salbutamol interacts with functional monomers by hydrogen bonds, and the salbutamol-imprinted polymers with a ratio of 1:4 (salbutamol/methacrylic acid) in acetonitrile had the highest stability. The salbutamol-imprinted polymers were prepared by precipitation polymerization. The experimental results indicated that the maximum adsorption capacity for salbutamol toward molecularly imprinted polymers was 7.33 mg/g, and the molecularly imprinted polymers had a higher selectivity for salbutamol than for norepinephrine and terbutaline sulfate. Herein, the studies can provide theoretical and experimental references for the salbutamol molecular imprinted system.

Emerging Pollutants - Part II: Treatment.

Emerging contaminants are considered as some substances of actual or potential threat to human health or environment, which include endocrine disruptors, pharmaceutical, personal care products, nanoparticles, antibiotic resistance genes and chemicals used in packaging products, etc. The disposal and treatment of emerging contaminants has become a key problem in the field of water pollution control. The purpose of this review is to summarize published researches on emerging pollutants treatment in 2014.

Recent remediation strategies for flame retardancy via nanoparticles.

This review article delves into the application of nanoparticles (NPs) in fire prevention, aiming to elucidate their specific contribution within the broader context of various fire prevention methods. While acknowledging established approaches such as fire safety principles, fire suppression systems, fire alarm systems, and the use of fire-retardant chemicals and safety equipment, this review focuses on the distinctive properties of NPs. The findings underscore the remarkable potential of NPs in controlling and mitigating fire propagation within both architectural structures and vehicles. Specifically, the primary emphasis lies in the impact of NPs on reducing oxygen levels, as assessed through the limiting oxygen index , a subject explored by various researchers. Furthermore, this review delves into the examination of combustion reduction rates facilitated by NPs, utilizing assessments of ignition time, heat release rate (HRR), and flammability tests (UL-94) on plastic materials. Beyond these aspects, the review evaluates the multifaceted role of NPs in achieving weight reduction and establishing fire-retardant properties. Additionally, it discusses the reduction of smoke, a significant contributor to environmental pollution and health risks. Among the nanoparticles investigated in this study, SiO2, MgAl, and nano hydrotalcite have demonstrated the best results in weight reduction, smoke reduction, and HRR, respectively. Meanwhile, Al2O3 has been identified as one of the least effective treated nanoparticles. Collectively, these findings significantly contribute to improving safety measures and reducing fire risks across a range of industries.

7-Benzyl-3-methyl-6-phenyl-imidazo[2,1-b][1,3]thia-zol-7-ium chloride 0.75-hydrate.

Theasymmetric unit of the title salt, C19H17N2S(+)·Cl(-)·0.75H2O, contains two symmetrically independent formula units of the carbenium salt along with three water mol-ecules. The water mol-ecules are only 50% occupated, and one of them is positioned in a hydro-phobic pocket not forming any hydrogen bonds. The conformation of the independent cations is very similar, with dihedral angles of 61.0 (2) and 61.5 (3)° between the benzene rings. They form quasi-centrosymmetric couples via π-π stacking inter-actions between the benzene and imidazo[2,1-b]thia-zole rings [centroid-centroid distances = 3.718 (3) and 3.663 (3) Å]. In the crystal, O-H⋯Cl hydrogen bonds lead to the formation of a helical anion-water chain along the c-axis direction. The cations connect to the anion-water chain through C-H⋯Cl inter-actions, generating a three-dimensional supra-molecular network. O-H⋯S hydrogen bonds and C-H⋯O inter-actions also occur.

Long-Term Impact of Domestic Violence on Individuals-An Empirical Study Based on Education, Health and Life Satisfaction.

This paper takes the China Health and Retirement Longitudinal Survey (CHARLS) as a sample to assess the long-term impacts of domestic violence experienced in childhood on individuals. First, from the four dimensions of injury from violence, negligent care, emotional abuse and witness to domestic violence, an indicator system for quantifying domestic violence is constructed. Second, the simultaneous equation of self-evaluation health and life satisfaction is estimated by the seemingly unrelated regression model. Starting with education, health and life satisfaction, the long-term impact of domestic violence experiences on individuals is quantitatively assessed, providing empirical evidence for preventing and curing domestic violence and healing trauma. The empirical research shows the following: (1) An experience of domestic violence significantly reduces educational achievements. Compared with the three dimensions of injury from violence, negligent care and witnessing domestic violence, emotional abuse has the greatest negative impact on educational achievements. (2) Domestic violence significantly reduces the self-assessed health level and life satisfaction and increases the subjective mental health risk. Based on the complexity and concealment of domestic violence, combined with empirical research conclusions, this paper proposes countermeasures to prevent and control domestic violence.

Light-acquisition traits link aboveground biomass and environment in inner saline-alkaline herbaceous marshes.

A functional response-effect approach could predict how environmental changes affect ecosystem functioning. However, few studies have applied this approach to inner saline-alkaline marsh ecosystems where soil saline-alkaline, flooding/drought and nutrients stresses threat ecological functioning. To disentangle the relationships between environmental conditions and ecosystem functioning, a total of 81 plots were investigated across 22 marsh sites dominated by Phragmites australis and Bolboschoenus planiculmis in Western Songnen Plain wetlands, China. For both plant communities combined, deep flooding supported communities with higher specific leaf area (SLA), plant height and leaf nitrogen (N) content but lower leaf thickness. On the contrary, high soil salt content induced low leaf N and phosphorus (P) content, SLA and plant height. Only light acquisition-related trait, plant height and SLA, was the key traits which determined the relationships between ecosystem functioning (aboveground biomass) and saline-alkaline wetland environment. Yet indirect key traits related nutrient and water acquisition such as leaf thickness, N and P content were also found, and mediated the response of aboveground biomass through the allometric relationships with plant height or SLA. For the individual species community, only plant height was the key trait shared by P. australis and B. planiculmis, indicating the universality of plant height as a key trait for grass and sedge plants to explain how ecosystem functioning responds to abiotic factors. Hence, our findings suggest that saltmarsh plants are more inclined to alter light-acquisition traits to mediate the response of ecosystem functioning to environmental changes and that plant height is a particularly useful trait to predict plant productivity in earth system models under future environmental changes in inner saline-alkaline wetlands.

Global trends of Vaccinia oncolytic virus therapy over the past two decades: Bibliometric and visual analysis.

Background: In recent years, the vaccinia oncolytic virus has entered the clinical trial stage of examination and shown good progress. It has many advantages, such as good safety, high oncolytic efficiency, and the regulation ability of the tumor microenvironment, and is expected to be successfully used in the clinical treatment of tumors in the future. However, no bibliometric analysis has so far been performed that generalizes horizontally across this field. Therefore, this study aims to assess the research status and trends in this field from a global perspective to help guide future research priorities. Methods: In this study, the literature related to vaccinia oncolytic virus published in English on Web of Science from 2002 to 2022 was retrieved, and the bibliometric indicators were analyzed using the Histcite. Pro 2.0 tool, while VOSviewer was used to visualize the research trends and hotspots in this field. Results: In total, 408 related studies were included. In the past 20 years, the number of related publications in this field has increased year by year, and breakthroughs were made in this field in 2008 and 2013. The research has grown rapidly since 2008, and will likely continue to expand in the years to come. The United States plays a leading role in this area. "MOLECULAR THERAPY-ONCOLYTICS", "MOLECULAR THERAPY" and "JOURNAL OF TRANSLATIONAL MEDICINE" are core journals that publish high-quality literature on the latest advances in the field. Some authors with numerous high-quality publications include Bell JC and Szalay AA. At present, the research hotspot in this field focus on the clinical application of vaccinia oncolytic virus. Conclusion: Overall, the number of vaccinia oncolytic virus-related studies is growing rapidly, in relation to which the United States is the most influential country. The clinical application of vaccinia oncolytic virus will affect the crucial development of future research.

Antidiabetic potential of Gymnemic acid mediated gold nanoparticles (Gym@AuNPs) on Streptozotocin-induced diabetic rats-An implication on in vivo approach.

Gymnemic acid is glycosides of triterpene with recognized and valuable applications for several chronic diseases, mainly diabetics. Despite this, it requires a delivery system in order to range its therapeutic target due to its limited solubility and bioavailability. Therefore, the Gymnemic acid mediated gold nanoparticles (Gym@AuNPs) was synthesised by eco-friendly approach. The synthesised Gym@AuNPs was confirmed by the colour change from light yellow to a deep ruby red. UV - visible spectroscopy results showed a strong narrow peak at 530 nm, confirming the controlled synthesis of monodispersed Gym@AuNPs. The reduction potential of standard Gymnemic acid (Gym) on synthesis of Gym@AuNPs was confirmed by using HPLC analysis. The spherical shaped Gym@AuNPs was observed by FESEM and HR-TEM studies with average size of 48.52 ± 5.53 nm. The XRD analysis exhibited a face-centered cubic (FCC) crystalline nature of Gym@AuNPs. The in vivo antidiabetic activity of Gym and Gym@AuNPs were validated using Streptozotocin induced diabetic Albino wistar rats. The Gym@AuNPs and Gym were regulates the glucose and lipid levels in experimental animals. The histopathology outcomes shown that the Gym@AuNPs were restoration of pancreatic islets cells in the animals. This investigation demonstrated that the Gym@AuNPs had the potential anti-diabetic properties.

Prolonged duration of epidural labour analgesia decreases the success rate of epidural anaesthesia for caesarean section.

OBJECTIVE: To summarise the process of conversion of epidural labour analgesia to anaesthesia for caesarean delivery and explore the relationship between duration of labour analgesia and conversion. METHODS: Parturients who underwent conversion from epidural labour analgesia to anaesthesia for caesarean delivery between May 2019 and April 2020 at the Chengdu Women's and Children's Central Hospital, Sichuan Maternal and Child Health Hospital, and Jinjiang District Maternal and Child Health Hospital were selected. If the position of the epidural catheter was correct and the effect was good, patients were converted to epidural surgical anaesthesia. If epidural labour analgesia was ineffective, spinal anaesthesia (SA) was administered immediately. For category-1 emergency caesarean sections, general anaesthesia (GA) was administered. RESULTS: A total of 1084 parturients underwent conversion. Of these, 19 (1.9%) received GA due to the initiation of category-1 emergency caesarean section. 704 (64.9%) were converted to epidural surgical anaesthesia, 2 (0.2%) had failed conversions and were administered GA before delivery, and 357 (32.9%) were converted to SA. Logistic regression analysis showed that prolonged duration of epidural labour analgesia ([Crude odds ratio (OR)=1.065; 95% confidence interval (CI), 1.037-1.094; p < .01]; [Adjusted OR = 1.060; 95% CI, 1.031-1.091; p < .01]) was an independent risk factor for conversion failure. A receiver operating characteristic curve constructed using duration of epidural labour analgesia showed that parturients with a duration of epidural labour analgesia ≥8 h, more frequently required a change of anaesthesia technique during conversion, and the relative risk of conversion failure was 1.54 (95% CI, 1.23-1.93; p < .01). CONCLUSION: Prolonged duration of epidural labour analgesia increases the possibility of having an invalid epidural catheter, resulting in an increased risk of conversion failure from epidural labour analgesia to epidural surgical anaesthesia. Further, this risk is higher when the time exceeds 8 h. KEY MESSAGESProlonged duration of epidural labour analgesia > 8 h is associated with conversion failure.If it is impossible to judge whether the conversion is successful immediately, spinal anaesthesia should be administered to minimise complications.

Fault Detection for T-S Fuzzy Discrete Systems in Finite-Frequency Domain.

This paper investigates the problem of fault detection for Takagi-Sugeno (T-S) fuzzy discrete systems in finite-frequency domain. By means of the T-S fuzzy model, both a fuzzy fault detection filter system and the dynamics of filtering error generator are constructed. Two finite-frequency performance indices are introduced to measure fault sensitivity and disturbance robustness. Faults are considered in a middle frequency domain, while disturbances are considered in another certain finite-frequency domain interval. By using the generalized Kalman-Yakubovic-Popov Lemma in a local linear system model, the design methods are presented in terms of solutions to a set of linear matrix inequalities, which can be readily solved via standard numerical software. The design problem is formulated as a two-objective optimization algorithm. A numerical example is given to illustrate the effectiveness and potential of the developed techniques.