ABSTRACT
The biogenesis of iron-sulfur (Fe/S) proteins entails the synthesis and trafficking of Fe/S clusters, followed by their insertion into target apoproteins. In eukaryotes, the multiple steps of biogenesis are accomplished by complex protein machineries in both mitochondria and cytosol. The underlying biochemical pathways have been elucidated over the past decades, yet the mechanisms of cytosolic [2Fe-2S] protein assembly have remained ill-defined. Similarly, the precise site of glutathione (GSH) requirement in cytosolic and nuclear Fe/S protein biogenesis is unclear, as is the molecular role of the GSH-dependent cytosolic monothiol glutaredoxins (cGrxs). Here, we investigated these questions in human and yeast cells by various in vivo approaches. [2Fe-2S] cluster assembly of cytosolic target apoproteins required the mitochondrial ISC machinery, the mitochondrial transporter Atm1/ABCB7 and GSH, yet occurred independently of both the CIA system and cGrxs. This mechanism was strikingly different from the ISC-, Atm1/ABCB7-, GSH-, and CIA-dependent assembly of cytosolic-nuclear [4Fe-4S] proteins. One notable exception to this cytosolic [2Fe-2S] protein maturation pathway defined here was yeast Apd1 which used the CIA system via binding to the CIA targeting complex through its C-terminal tryptophan. cGrxs, although attributed as [2Fe-2S] cluster chaperones or trafficking proteins, were not essential in vivo for delivering [2Fe-2S] clusters to either CIA components or target apoproteins. Finally, the most critical GSH requirement was assigned to Atm1-dependent export, i.e. a step before GSH-dependent cGrxs function. Our findings extend the general model of eukaryotic Fe/S protein biogenesis by adding the molecular requirements for cytosolic [2Fe-2S] protein maturation.
Subject(s)
Cytosol , Glutaredoxins , Glutathione , Iron-Sulfur Proteins , Mitochondria , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae , Cytosol/metabolism , Iron-Sulfur Proteins/metabolism , Humans , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/metabolism , Saccharomyces cerevisiae Proteins/genetics , Glutathione/metabolism , Mitochondria/metabolism , Glutaredoxins/metabolism , Glutaredoxins/genetics , ATP-Binding Cassette Transporters/metabolism , Mitochondrial Proteins/metabolismABSTRACT
The physiological roles of the intracellular iron and redox regulatory systems are intimately linked. Iron is an essential trace element for most organisms, yet elevated cellular iron levels are a potent generator and amplifier of reactive oxygen species and redox stress. Proteins binding iron or iron-sulfur (Fe/S) clusters, are particularly sensitive to oxidative damage and require protection from the cellular oxidative stress protection systems. In addition, key components of these systems, most prominently glutathione and monothiol glutaredoxins are involved in the biogenesis of cellular Fe/S proteins. In this review, we address the biochemical role of glutathione and glutaredoxins in cellular Fe/S protein assembly in eukaryotic cells. We also summarize the recent developments in the role of cytosolic glutaredoxins in iron metabolism, in particular the regulation of fungal iron homeostasis. Finally, we discuss recent insights into the interplay of the cellular thiol redox balance and oxygen with that of Fe/S protein biogenesis in eukaryotes.
Subject(s)
Glutaredoxins/metabolism , Iron-Sulfur Proteins/metabolism , Iron/metabolism , Saccharomyces cerevisiae Proteins/metabolism , Saccharomyces cerevisiae/metabolism , Oxidation-ReductionABSTRACT
Allosteric communication between two ligand-binding sites in a protein is a central aspect of biological regulation that remains mechanistically unclear. Here we show that perturbations in equilibrium picosecond-nanosecond motions impact zinc (Zn)-induced allosteric inhibition of DNA binding by the Zn efflux repressor CzrA (chromosomal zinc-regulated repressor). DNA binding leads to an unanticipated increase in methyl side-chain flexibility and thus stabilizes the complex entropically; Zn binding redistributes these motions, inhibiting formation of the DNA complex by restricting coupled fast motions and concerted slower motions. Allosterically impaired CzrA mutants are characterized by distinct nonnative fast internal dynamics "fingerprints" upon Zn binding, and DNA binding is weakly regulated. We demonstrate the predictive power of the wild-type dynamics fingerprint to identify key residues in dynamics-driven allostery. We propose that driving forces arising from dynamics can be harnessed by nature to evolve new allosteric ligand specificities in a compact molecular scaffold.
Subject(s)
Bacterial Proteins/chemistry , DNA-Binding Proteins/chemistry , Entropy , Zinc/metabolism , Allosteric Regulation , Models, Molecular , Protein Binding , Protein Conformation , Staphylococcus aureus/metabolism , TemperatureABSTRACT
The biogenesis of iron-sulfur (Fe/S) proteins in eukaryotes is a multistage, multicompartment process that is essential for a broad range of cellular functions, including genome maintenance, protein translation, energy conversion, and the antiviral response. Genetic and cell biological studies over almost 2 decades have revealed some 30 proteins involved in the synthesis of cellular [2Fe-2S] and [4Fe-4S] clusters and their incorporation into numerous apoproteins. Mechanistic aspects of Fe/S protein biogenesis continue to be elucidated by biochemical and ultrastructural investigations. Here, we review recent developments in the pursuit of constructing a comprehensive model of Fe/S protein assembly in the mitochondrion.
Subject(s)
Gene Expression Regulation, Enzymologic , Iron-Sulfur Proteins/metabolism , Mitochondria/metabolism , Models, Biological , Models, Molecular , Acyl Carrier Protein/chemistry , Acyl Carrier Protein/genetics , Acyl Carrier Protein/metabolism , Adrenodoxin/chemistry , Adrenodoxin/genetics , Adrenodoxin/metabolism , Animals , Apoenzymes/chemistry , Apoenzymes/genetics , Apoenzymes/metabolism , Humans , Iron-Binding Proteins/chemistry , Iron-Binding Proteins/genetics , Iron-Binding Proteins/metabolism , Iron-Sulfur Proteins/chemistry , Iron-Sulfur Proteins/genetics , Mitochondria/enzymology , Mitochondrial Proteins/chemistry , Mitochondrial Proteins/genetics , Mitochondrial Proteins/metabolism , Protein Conformation , Protein Folding , Protein Multimerization , Protein Transport , Saccharomyces cerevisiae Proteins/chemistry , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism , Species Specificity , Sulfurtransferases/chemistry , Sulfurtransferases/genetics , Sulfurtransferases/metabolism , FrataxinABSTRACT
Oxygen-containing mononuclear iron species--iron(III)-peroxo, iron(III)-hydroperoxo and iron(IV)-oxo--are key intermediates in the catalytic activation of dioxygen by iron-containing metalloenzymes. It has been difficult to generate synthetic analogues of these three active iron-oxygen species in identical host complexes, which is necessary to elucidate changes to the structure of the iron centre during catalysis and the factors that control their chemical reactivities with substrates. Here we report the high-resolution crystal structure of a mononuclear non-haem side-on iron(III)-peroxo complex, [Fe(III)(TMC)(OO)](+). We also report a series of chemical reactions in which this iron(III)-peroxo complex is cleanly converted to the iron(III)-hydroperoxo complex, [Fe(III)(TMC)(OOH)](2+), via a short-lived intermediate on protonation. This iron(III)-hydroperoxo complex then cleanly converts to the ferryl complex, [Fe(IV)(TMC)(O)](2+), via homolytic O-O bond cleavage of the iron(III)-hydroperoxo species. All three of these iron species--the three most biologically relevant iron-oxygen intermediates--have been spectroscopically characterized; we note that they have been obtained using a simple macrocyclic ligand. We have performed relative reactivity studies on these three iron species which reveal that the iron(III)-hydroperoxo complex is the most reactive of the three in the deformylation of aldehydes and that it has a similar reactivity to the iron(IV)-oxo complex in C-H bond activation of alkylaromatics. These reactivity results demonstrate that iron(III)-hydroperoxo species are viable oxidants in both nucleophilic and electrophilic reactions by iron-containing enzymes.
Subject(s)
Hydrogen Peroxide/chemistry , Iron/chemistry , Aldehydes/metabolism , Crystallography, X-Ray , Enzymes/chemistry , Enzymes/metabolism , Hydrogen Peroxide/metabolism , Iron/metabolism , Ligands , Models, Molecular , Nonheme Iron Proteins/chemistry , Nonheme Iron Proteins/metabolism , Oxygen/chemistry , Oxygen/metabolismABSTRACT
The purpose of this study was to determine metal ion levels in central visual system structures of the DBA/2J mouse model of glaucoma. We used inductively coupled plasma mass spectrometry (ICP-MS) to measure levels of iron (Fe), copper (Cu), zinc (Zn), magnesium (Mg), manganese (Mn), and calcium (Ca) in the retina and retinal projection of 5-month (pre-glaucomatous) and 10-month (glaucomatous) old DBA/2J mice and age-matched C57BL/6J controls. We used microbeam X-ray fluorescence (µ-XRF) spectrometry to determine the spatial distribution of Fe, Zn, and Cu in the superior colliculus (SC), which is the major retinal target in rodents and one of the earliest sites of pathology in the DBA/2J mouse. Our ICP-MS experiments showed that glaucomatous DBA/2J had lower retinal Fe concentrations than pre-glaucomatous DBA/2J and age-matched C57BL/6J mice. Pre-glaucomatous DBA/2J retina had greater Mg, Ca, and Zn concentrations than glaucomatous DBA/2J and greater Mg and Ca than age-matched controls. Retinal Mn levels were significantly deficient in glaucomatous DBA/2J mice compared to aged-matched C57BL/6J and pre-glaucomatous DBA/2J mice. Regardless of age, the SC of C57BL/6J mice contained greater Fe, Mg, Mn, and Zn concentrations than the SC of DBA/2J mice. Greater Fe concentrations were measured by µ-XRF in both the superficial and deep SC of C57BL/6J mice than in DBA/2J mice. For the first time, we show direct measurement of metal concentrations in central visual system structures affected in glaucoma and present evidence for strain-related differences in metal content that may be specific to glaucomatous pathology.
Subject(s)
Glaucoma/metabolism , Metals/analysis , Mice, Inbred DBA/metabolism , Nerve Degeneration/metabolism , Visual Pathways/chemistry , Animals , Cerebellum/chemistry , Glaucoma/genetics , Mass Spectrometry , Mice , Mice, Inbred C57BL , Mice, Inbred DBA/genetics , Models, Animal , Optic Nerve/chemistry , Retina/chemistry , Spectrometry, X-Ray Emission , Superior Colliculi/chemistryABSTRACT
Monocercomonoides exilis is the first known amitochondriate eukaryote. Loss of mitochondria in M. exilis ocurred after the replacement of the essential mitochondrial iron-sulfur cluster (ISC) assembly machinery by a unique, bacteria-derived, cytosolic SUF system. It has been hypothesized that the MeSuf pathway, in cooperation with proteins of the cytosolic iron-sulfur protein assembly (CIA) system, is responsible for the biogenesis of FeS clusters in M. exilis, yet biochemical evidence is pending. Here, we address the M. exilis MeSuf system and show that SUF genes, individually or in tandem, support the loading of iron-sulfur (FeS) clusters into the reporter protein IscR in Escherichia coli. The Suf proteins MeSufB, MeSufC, and MeSufDSU interact in vivo with one another and with Suf proteins of E. coli. In vitro, the M. exilis Suf proteins form large complexes of varying composition and hence may function as a dynamic biosynthetic system in the protist. The putative FeS cluster scaffold MeSufB-MeSufC (MeSufBC) forms multiple oligomeric complexes, some of which bind FeS clusters and form selectively only in the presence of adenosine nucleotides. The multi-domain fusion protein MeSufDSU binds a PLP cofactor and can form higher-order complexes with MeSufB and MeSufC. Our work demonstrates the biochemical property of M. exilis Suf proteins to act as a functional FeS cluster assembly system and provides insights into the molecular mechanism of this unique eukaryotic SUF system.
ABSTRACT
The accumulation of metal ions and amyloid-ß (Aß) aggregates found in the brain of patients with Alzheimer's disease (AD) has been suggested to be involved in AD pathogenesis. To investigate metal-Aß-associated pathways in AD, development of chemical tools to target metal-Aß species is desired. Only a few efforts, however, have been reported. Here, we report bifunctional small molecules, N-(pyridin-2-ylmethyl)aniline (L2-a) and N(1),N(1)-dimethyl-N(4)-(pyridin-2-ylmethyl)benzene-1,4-diamine (L2-b) that can interact with both metal ions and Aß species, as determined by spectroscopic methods including high-resolution NMR spectroscopy. Using the bifunctional compound L2-b, metal-induced Aß aggregation and neurotoxicity were modulated in vitro as well as in human neuroblastoma cells. Furthermore, treatment of human AD brain tissue homogenates containing metal ions and Aß species with L2-b showed disassembly of Aß aggregates. Therefore, our studies presented herein demonstrate the value of bifunctional compounds as chemical tools for investigating metal-Aß-associated events and their mechanisms in the development and pathogenesis of AD and as potential therapeutics.
Subject(s)
Amyloid beta-Peptides/metabolism , Copper/pharmacology , Phenylenediamines/pharmacology , Pyridines/pharmacology , Zinc/pharmacology , Alzheimer Disease , Amyloid beta-Peptides/chemistry , Cell Line, Tumor , Copper/chemistry , Humans , Phenylenediamines/chemistry , Pyridines/chemistry , Zinc/chemistryABSTRACT
Systematic studies have revealed interactions between components of the Hsp90 chaperone system and Fe/S protein biogenesis or iron regulation. In addition, two chloroplast-localized DnaJ-like proteins, DJA5 and DJA6, function as specific iron donors in plastidial Fe/S protein biogenesis. Here, we used Saccharomyces cerevisiae to study the impact of both the Hsp90 chaperone and the yeast DJA5-DJA6 homologs, the essential cytosolic Ydj1, and the mitochondrial Mdj1, on cellular iron-related processes. Despite severe phenotypes induced upon depletion of these crucial proteins, there was no critical in vivo impact on Fe/S protein biogenesis or iron regulation. Importantly, unlike the plant DJA5-DJA6 iron chaperones, Ydj1 and Mdj1 did not bind iron in vivo, suggesting that these proteins use zinc for function under normal physiological conditions.
Subject(s)
Iron-Sulfur Proteins , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , Iron-Sulfur Proteins/genetics , Iron-Sulfur Proteins/metabolism , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism , Iron/metabolism , Mitochondrial Proteins/metabolism , Molecular Chaperones/metabolism , HSP90 Heat-Shock Proteins/genetics , HSP90 Heat-Shock Proteins/metabolismABSTRACT
Amyloid-ß (Aß) peptides and their metal-associated aggregated states have been implicated in the pathogenesis of Alzheimer's disease (AD). Although the etiology of AD remains uncertain, understanding the role of metal-Aß species could provide insights into the onset and development of the disease. To unravel this, bifunctional small molecules that can specifically target and modulate metal-Aß species have been developed, which could serve as suitable chemical tools for investigating metal-Aß-associated events in AD. Through a rational structure-based design principle involving the incorporation of a metal binding site into the structure of an Aß interacting molecule, we devised stilbene derivatives (L1-a and L1-b) and demonstrated their reactivity toward metal-Aß species. In particular, the dual functions of compounds with different structural features (e.g., with or without a dimethylamino group) were explored by UV-vis, X-ray crystallography, high-resolution 2D NMR, and docking studies. Enhanced bifunctionality of compounds provided greater effects on metal-induced Aß aggregation and neurotoxicity in vitro and in living cells. Mechanistic investigations of the reaction of L1-a and L1-b with Zn(2+)-Aß species by UV-vis and 2D NMR suggest that metal chelation with ligand and/or metal-ligand interaction with the Aß peptide may be driving factors for the observed modulation of metal-Aß aggregation pathways. Overall, the studies presented herein demonstrate the importance of a structure-interaction-reactivity relationship for designing small molecules to target metal-Aß species allowing for the modulation of metal-induced Aß reactivity and neurotoxicity.
Subject(s)
Alzheimer Disease/metabolism , Amyloid beta-Peptides/metabolism , Chelating Agents/chemical synthesis , Molecular Probes/chemical synthesis , Stilbenes/chemical synthesis , Alzheimer Disease/physiopathology , Amyloid beta-Peptides/chemistry , Cell Line, Tumor , Cell Survival/drug effects , Chelating Agents/metabolism , Chelating Agents/pharmacology , Copper/chemistry , Copper/metabolism , Crystallography, X-Ray , Drug Design , Humans , Iron/chemistry , Iron/metabolism , Ligands , Magnetic Resonance Spectroscopy , Microscopy, Electron, Transmission , Models, Molecular , Molecular Probes/metabolism , Molecular Probes/pharmacology , Protein Conformation/drug effects , Stilbenes/metabolism , Stilbenes/pharmacology , Structure-Activity Relationship , Zinc/chemistry , Zinc/metabolismABSTRACT
Iron-sulfur (Fe/S) proteins are present in virtually all living organisms and are involved in numerous cellular processes such as respiration, photosynthesis, metabolic reactions, nitrogen fixation, radical biochemistry, protein synthesis, antiviral defense, and genome maintenance. Their versatile functions may go back to the proposed role of their Fe/S cofactors in the origin of life as efficient catalysts and electron carriers. More than two decades ago, it was discovered that the in vivo synthesis of cellular Fe/S clusters and their integration into polypeptide chains requires assistance by complex proteinaceous machineries, despite the fact that Fe/S proteins can be assembled chemically in vitro. In prokaryotes, three Fe/S protein biogenesis systems are known; ISC, SUF, and the more specialized NIF. The former two systems have been transferred by endosymbiosis from bacteria to mitochondria and plastids, respectively, of eukaryotes. In their cytosol, eukaryotes use the CIA machinery for the biogenesis of cytosolic and nuclear Fe/S proteins. Despite the structural diversity of the protein constituents of these four machineries, general mechanistic concepts underlie the complex process of Fe/S protein biogenesis. This review provides a comprehensive and comparative overview of the various known biogenesis systems in Biology, and summarizes their common or diverging molecular mechanisms, thereby illustrating both the conservation and diverse adaptions of these four machineries during evolution and under different lifestyles. Knowledge of these fundamental biochemical pathways is not only of basic scientific interest, but is important for the understanding of human 'Fe/S diseases' and can be used in biotechnology.
Subject(s)
Iron-Sulfur Proteins/genetics , Mitochondria/genetics , Photosynthesis/genetics , Symbiosis/genetics , Bacteria/genetics , Cytosol/metabolism , Humans , Iron/metabolism , Iron-Sulfur Proteins/metabolism , Mitochondria/metabolism , Respiration/genetics , Saccharomyces cerevisiae/genetics , Sulfur/metabolismABSTRACT
Our design of bifunctional metal chelators as chemical probes and potential therapeutics for Alzheimer's disease (AD) is based on the incorporation of a metal binding moiety into structural frameworks of Abeta aggregate-imaging agents. Using this strategy, two compounds 2-[4-(dimethylamino)phenyl]imidazo[1,2-a]pyridine-8-ol (1) and N(1),N(1)-dimethyl-N(4)-(pyridin-2-ylmethylene)benzene-1,4-diamine (2) were prepared and characterized. The bifunctionality for metal chelation and Abeta interaction of 1 and 2 was verified by spectroscopic methods. Furthermore, the reactivity of 1 and 2 with Cu(II)-associated Abeta aggregates was investigated. The modulation of Cu(II)-triggered Abeta aggregation by 1 and 2 was found to be more effective than that by the known metal chelating agents CQ, EDTA, and phen. These studies suggest a new class of multifunctional molecules for the development of chemical tools to unravel metal-associated events in AD and potential therapeutic agents for metal-ion chelation therapy.
Subject(s)
Alzheimer Disease/metabolism , Amyloid beta-Peptides/drug effects , Chelating Agents/pharmacology , Copper/metabolism , Imidazoles/pharmacology , Phenylenediamines/pharmacology , Pyridines/pharmacology , Alzheimer Disease/drug therapy , Amyloid beta-Peptides/chemistry , Amyloid beta-Peptides/metabolism , Cell Line, Tumor , Chelating Agents/chemistry , Chelating Agents/therapeutic use , Copper/chemistry , Drug Design , Humans , Imidazoles/chemistry , Imidazoles/therapeutic use , Phenylenediamines/chemistry , Phenylenediamines/therapeutic use , Pyridines/chemistry , Pyridines/therapeutic useABSTRACT
Ironsulfur (Fe/S) clusters are versatile inorganic cofactors that play central roles in essential cellular functions, from respiration to genome stability. >30 proteins involved in Fe/S protein biogenesis in eukaryotes are known, many of which bind clusters via cysteine residues. This opens up the possibility that the thiol-reducing glutaredoxin and thioredoxin systems are required at both the Fe/S biogenesis and target protein level to counteract thiol oxidation. To address the possible interplay of thiol redox chemistry and Fe/S protein biogenesis, we have characterized the status of the mitochondrial (ISC) and cytosolic (CIA) Fe/S protein assembly machineries in Saccharomyces cerevisiae mutants in which the three partially redundant glutathione (Glr1) and thioredoxin (Trr1 and Trr2) oxidoreductases have been inactivated in either mitochondria, cytosol, or both compartments. Cells devoid of mitochondrial oxidoreductases maintained a functional mitochondrial ISC machinery and showed no altered iron homeostasis despite a non-functional complex II of the respiratory chain due to redox-specific defects. In cells that lack either cytosolic or total cellular thiol reducing capacity, both the ISC system and iron homeostasis were normal, yet cytosolic and nuclear Fe/S target proteins were not matured. This dysfunction could be attributed to a failure in the assembly of [4Fe4S] clusters in the CIA factor Nar1, even though Nar1 maintained robust protein levels and stable interactions with later-acting CIA components. Overall, our analysis has uncovered a hitherto unknown thiol-dependence of the CIA machinery and has demonstrated the surprisingly varying sensitivity of Fe/S proteins to thiol oxidation.
Subject(s)
Iron-Sulfur Proteins/biosynthesis , Iron-Sulfur Proteins/metabolism , Cell Nucleus/metabolism , Cytosol/metabolism , Genomic Instability , Glutaredoxins/metabolism , Homeostasis , Iron/metabolism , Iron-Sulfur Proteins/genetics , Mitochondria/metabolism , Oxidation-Reduction , Protein Transport , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae Proteins/genetics , Sulfhydryl Compounds/metabolism , Sulfur/metabolism , Thioredoxins/metabolismABSTRACT
In this issue of Cell Chemical Biology, Pandey et al. (2018) identified that mitochondrial cysteine desulfurase provides the sulfur species used for tRNALys, tRNAGlu, and tRNAGln thiouridine modification in the cytoplasm. A low-mass sulfur species is exported by the mitochondrial Atm1 transporter and utilized in the thio-modifications.
Subject(s)
Mitochondria , RNA, Transfer , Cytosol , Sulfur , ThiouridineSubject(s)
Alzheimer Disease , Amyloid beta-Peptides/drug effects , Chlorides/chemistry , Copper/chemistry , Flavonoids/chemistry , Zinc Compounds/chemistry , Alzheimer Disease/physiopathology , Amino Acid Motifs , Cell Line, Tumor , Cell Survival , Flavonoids/pharmacology , Humans , Molecular StructureABSTRACT
Zinc (Zn) is an essential metal that vertebrates sequester from pathogens to protect against infection. Investigating the opportunistic pathogen Acinetobacter baumannii's response to Zn starvation, we identified a putative Zn metallochaperone, ZigA, which binds Zn and is required for bacterial growth under Zn-limiting conditions and for disseminated infection in mice. ZigA is encoded adjacent to the histidine (His) utilization (Hut) system. The His ammonia-lyase HutH binds Zn very tightly only in the presence of high His and makes Zn bioavailable through His catabolism. The released Zn enables A. baumannii to combat host-imposed Zn starvation. These results demonstrate that A. baumannii employs several mechanisms to ensure bioavailability of Zn during infection, with ZigA functioning predominately during Zn starvation, but HutH operating in both Zn-deplete and -replete conditions to mobilize a labile His-Zn pool.
Subject(s)
Acinetobacter baumannii/metabolism , Zinc/deficiency , Acinetobacter Infections/microbiology , Acinetobacter baumannii/genetics , Acinetobacter baumannii/growth & development , Animals , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Chlorides/metabolism , GTP Phosphohydrolases/metabolism , Gene Expression Regulation, Bacterial , Genes, Bacterial , Histidine/metabolism , Histidine Ammonia-Lyase/metabolism , Metallochaperones/genetics , Metallochaperones/metabolism , Mice , Mice, Inbred C57BL , Mutation , Zinc/metabolism , Zinc Compounds/metabolismABSTRACT
Copper and zinc homeostasis systems in pathogenic bacteria are required to resist host efforts to manipulate the availability and toxicity of these metal ions. Central to this microbial adaptive response is the involvement of metal-trafficking and metal-sensing proteins that ultimately exercise control of metal speciation in the cell. Cu-specific and Zn-specific metalloregulatory proteins regulate the transcription of metal-responsive genes while metallochaperones and related proteins ensure that these metals are appropriately buffered by the intracellular milieu and delivered to correct intracellular targets. In this review, we summarize recent findings on how bacterial pathogens mount a metal-specific response to derail host efforts to win the 'fight over metals.'
Subject(s)
Bacteria/metabolism , Copper/metabolism , Homeostasis , Zinc/metabolism , Bacteria/chemistry , Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Biological Transport , Copper/chemistry , Humans , Zinc/chemistryABSTRACT
Metal ions associated with amyloid-ß (Aß) species have been suggested to be involved in neurodegeneration leading to the progression of Alzheimer's disease (AD). The role of metal-involved Aß species in AD neuropathogenesis, however, is not fully elucidated. In order to advance this understanding and contribute to the therapeutic development for AD, the rational structure-based design of small molecules that specifically target metal ions surrounded by Aß species has recently received increased attention. To date, only a few compounds have been fashioned for this purpose. Herein, we report the design strategy, synthesis, characterization, and reactivity of new bifunctional IMPY derivatives K1 and K2. Using UV-vis and high-resolution two-dimensional (2D) NMR spectroscopy, the bifunctionality of K1 and K2 (metal chelation and Aß interaction) was confirmed. These bifunctional IMPY derivatives showed preferential reactivity toward metal-induced Aß aggregation over metal-free conditions in both in vitro inhibition and disaggregation experiments. Taken together, this study provides another example of a bifunctional small molecule framework that can target metal ions associated with Aß species.
Subject(s)
Alzheimer Disease/drug therapy , Amyloid beta-Peptides/metabolism , Chelating Agents/chemistry , Chelating Agents/pharmacology , Metals/metabolism , Pyridines/chemistry , Pyridines/pharmacology , Alzheimer Disease/metabolism , Amyloid beta-Peptides/ultrastructure , Drug Design , Humans , Pyrazoles/chemistryABSTRACT
Alzheimer's disease (AD) is a multifactorial neurodegenerative disease related to the deposition of aggregated amyloid-ß (Aß) peptides in the brain. It has been proposed that metal ion dyshomeostasis and miscompartmentalization contribute to AD progression, especially as metal ions (e.g., Cu(II) and Zn(II)) found in Aß plaques of the diseased brain can bind to Aß and be linked to aggregation and neurotoxicity. The role of metal ions in AD pathogenesis, however, is uncertain. To accelerate understanding in this area and contribute to therapeutic development, recent efforts to devise suitable chemical reagents that can target metal ions associated with Aß have been made using rational structure-based design that combines two functions (metal chelation and Aß interaction) in the same molecule. This paper presents bifunctional compounds developed by two different design strategies (linkage or incorporation) and discusses progress in their applications as chemical tools and/or potential therapeutics.