ABSTRACT
Straelensia cynotis is a trombidioid mite that causes painful, usually nonpruritic nodular dermatitis mainly in the dorsal region of dogs. This case report describes the first observation of feline straelensiosis in Europe with clinicopathological findings. Molecular characterisation of the parasite was performed and compared with mites collected from dogs.
Straelensia cynotis est un acarien trombidioïde qui provoque une dermatite nodulaire douloureuse, généralement non prurigineuse, principalement dans la région dorsale des chiens. Ce cas constitue la première observation de straelensiose féline en Europe avec des données clinicopathologiques. L'identification moléculaire du parasite a été réalisée et comparée à celle d'acariens prélevés sur des chiens.
Straelensia cynotis es un ácaro trombidioide que causa dermatitis nodular dolorosa, generalmente no pruriginosa, principalmente en la región dorsal de los perros. Este informe de caso describe la primera observación de estraelensiosis felina en Europa con hallazgos clínico-patológicos. Se realizó la caracterización molecular del parásito y se comparó con ácaros recolectados de perros.
Straelensia cynotis é um ácaro trombiculídeo que causa dermatite nodular dolorosa e geralmente não pruriginosa principalmente na região dorsal de cães. Este relato de caso descreve a primeira observação de stralensiose felina na Europa com achados clinicopatológicos. A caracterização molecular do parasita foi realizada e comparada com ácaros coletados de cães.
Subject(s)
Cat Diseases , Dermatitis , Dog Diseases , Mite Infestations , Mites , Cats , Animals , Dogs , Dog Diseases/pathology , Mites/genetics , Europe , Dermatitis/veterinary , Mite Infestations/veterinary , Mite Infestations/parasitology , Cat Diseases/parasitologyABSTRACT
Clinical history: An outbreak of intense pruritus and weight loss in a herd of 40 alpacas (Vicugna pacos) in the south-west of France was investigated after the death of 14 adults. One alpaca was referred to a veterinary teaching hospital for diagnosis and treatment but died soon after and one of the dead alpacas was submitted for necropsy. Clinical findings: The remaining alpacas were intensely pruritic with variably severe and extensive alopecia, erythema, lichenification and crusting on the face, ventral abdomen and distal limbs. Superficial skin scrapes from five animals revealed large numbers of Sarcoptes scabiei mites, and less frequent and numerous Chorioptes bovis mites. Coproscopic examinations revealed a median of 1,350 (min 500, max 8800) strongyle epg. The alpaca admitted for treatment was anaemic and hypoalbuminaemic. Skin scrapes revealed copious S. scabiei and C. bovis mites. The two alpacas examined post-mortem had similar skin lesions to those examined on-farm and were cachexic. One had lung lesions attributed to protostrongylid infestation and its liver contained numerous Dicrocoelium spp. adults. Diagnosis: Sarcoptic and chorioptic mange with secondary superficial bacterial skin infection, associated with severe internal parasitism and underfeeding. Treatment and outcome: All 25 alpacas were treated topically with a 3% chlorhexidine shampoo followed by a 0.025% amitraz wash at the initial visit and then 1, 2, 3, 7 and 9 weeks later. A systemic treatment with S/C 500â µg/kg ivermectin was administered at the initial visit and then 2, 7 and 9 weeks later. The alpacas were treated orally with 50â mg/kg praziquantel to control dicrocoeliosis. Nutritional measures, including increased pasture area and supplemental feeding were simultaneously implemented. Pruritus was reduced 1 week after the start of treatment and had resolved after 2 weeks. After 9 weeks, skin lesions were markedly improved. Six months after the initial visit, skin lesions entirely resolved and superficial skin scrapes, taken from half of the animals, were negative for mites. Clinical relevance: This is the first report of the use of two acaricides combined with a chlorhexidine shampoo to successfully treat simultaneous sarcoptic and chorioptic mange in alpacas.
Subject(s)
Camelids, New World/parasitology , Insecticides/therapeutic use , Ivermectin/therapeutic use , Scabies/veterinary , Toluidines/therapeutic use , Administration, Topical , Animals , Anthelmintics/therapeutic use , Anti-Infective Agents, Local/therapeutic use , Chlorhexidine/administration & dosage , Chlorhexidine/therapeutic use , Dicrocoeliasis/drug therapy , Dicrocoeliasis/veterinary , Drug Therapy, Combination , Female , Injections, Subcutaneous/veterinary , Insecticides/administration & dosage , Ivermectin/administration & dosage , Male , Praziquantel/therapeutic use , Scabies/drug therapy , Scabies/parasitology , Toluidines/administration & dosageABSTRACT
Canine sarcoptic mange is a highly pruritic and contagious skin disease caused by the mite Sarcoptes scabiei var. canis. This case series describes the clinical, parasitological, and serological follow-up of a cohort of eight adult Saint Bernard dogs with confirmed sarcoptic mange, treated orally with lotilaner. Dogs were evaluated initially and after 14 days and 1, 2, 3, 4, 6, and 12 months for skin lesions, pruritus severity, presence of parasites, and Sarcoptes-IgG levels. A serological indoor allergy panel (IgE) was obtained for seven dogs at day 0 and repeated 12 months later in five dogs to assess potential cross-reactivity between S. scabiei and environmental allergens. Lotilaner was administered to each dog according to the manufacturer's instructions and was repeated after one and two months without any concurrent therapeutic measure or modification of the husbandry conditions. Pruritus ceased after two weeks. The cutaneous score was reduced by 47%, and skin scrapings were negative for all but three animals. All skin scrapings were negative after one month. Lesions were absent after two months. Serological levels decreased gradually, but more slowly than the skin lesions, and two dogs out of six remained positive in the absence of skin lesions or symptoms. All dogs initially tested positive for dust mites and/or storage mites. The IgE titres remained unchanged 12 months later in the five tested dogs. This case report demonstrates the efficacy of lotilaner on scabies in a cohort of infested dogs under natural conditions and the potential antigenic cross-reaction of S. scabiei with house dust and storage mites.
ABSTRACT
Canine haircoat quality, the time course of incorporation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) into the canine erythrocyte membrane, changes in total lipids content on hair shafts and of neutral lipids on the skin surface, were investigated after administrating an n-3 fatty acids supplement. Twenty-four dogs with poor haircoat received a placebo or n-3 oil capsules (110 mg EPA/68 mg DHA) daily for 90 days. Clinical assessments and tissue sampling were performed monthly from day (D)0 to D180. The clinical score in treated dogs was significantly reduced from D60, then attained a plateau and returned to baseline levels on D180. EPA and DHA contents in the erythrocyte membrane increased significantly from D30 and decreased rapidly after supplement withdrawal in treated dogs. Total lipids on the hair shaft increased progressively in the supplemented group. EPA/DHA supplements impact blood and hair fatty acids important for haircoat quality in dogs.
Subject(s)
Animal Fur/metabolism , Dietary Supplements , Erythrocyte Membrane/metabolism , Fatty Acids, Omega-3/pharmacology , Skin/metabolism , Animals , Dogs , Double-Blind Method , Female , Male , Prospective StudiesABSTRACT
A new antimicrobial ear cleanser was evaluated for the treatment of bacterial and yeast ear infection in dogs. Forty-five dogs with erythemato-ceruminous or purulent otitis externa were randomly allocated to two treatment groups: reference ear cleanser (Epiotic, Virbac) or test ear cleanser (Epiotic Advanced, Virbac). Ear cleansing was performed twice daily for 2 weeks, and no other treatment was allowed. By week 2, clinical (exudate quantity, erythema, stenosis, excoriation, and odor) and discomfort (pain, ear scratching, and head shaking) scores were significantly decreased (P < .0001 for all) and no microbial overgrowth could be detected in 25 (64.1%) and 32 (68.1%) ears treated with Epiotic and Epiotic Advanced, respectively. The new pH-balanced, propylene glycol-free test ear cleanser, which incorporates microbial adhesin-blocking carbohydrates, proved as effective as the reference acidic formula.
Subject(s)
Anti-Infective Agents, Local/administration & dosage , Dog Diseases/drug therapy , Otitis Externa/veterinary , Administration, Topical , Animals , Bacterial Adhesion , Dog Diseases/microbiology , Dog Diseases/pathology , Dogs , Drug Therapy, Combination , Ear Canal , Female , Male , Otitis Externa/drug therapy , Treatment OutcomeABSTRACT
The authors report 10 cases of localised sarcoptic mange in dogs. In each case, lesions were localised to one precise area of the skin. Pruritus was present in nine cases and absent in one. Affected areas were the feet (one case), the face and/or the pinnae (six cases), the abdominal skin (one case), the flank (one case) and the lumbar area (one case). The types of lesions were erythema, papules, lichenification, scales, crusts and alopecia. Parasites were found in all cases except one, in which anti-immunoglobulin G Sarcoptes serology was positive. The acaricidal treatments given were lindane, ivermectin or selamectin and were all successful.
Subject(s)
Dog Diseases/pathology , Insecticides/therapeutic use , Sarcoptes scabiei/drug effects , Scabies/veterinary , Alopecia/epidemiology , Alopecia/parasitology , Alopecia/pathology , Alopecia/veterinary , Animals , Dog Diseases/drug therapy , Dogs , Female , Hexachlorocyclohexane/therapeutic use , Ivermectin/analogs & derivatives , Ivermectin/therapeutic use , Male , Pruritus/epidemiology , Pruritus/parasitology , Pruritus/pathology , Pruritus/veterinary , Retrospective Studies , Scabies/drug therapy , Scabies/pathology , Skin/parasitology , Skin/pathology , Treatment OutcomeABSTRACT
Three experiments were conducted on cats to evaluate precocity and duration of the first blood meal of Ctenocephalides felis felis (Bouché). Percentage of engorged fleas was calculated for fleas held on cats for 5, 15, 30, and 60 min. Duration of first blood meal was also measured for individual fleas confined on cats. When fleas are free in the hair coat, 24.9% are engorged after 5 min and 97.2% are engorged after 1 h. Fleas confined to a vial on the cats fed significantly sooner; 60% of females were engorged within 5 min. The mean delay between deposition and biting for fleas, which began feeding within 15 min, was 24 s +/- 31 s for females and 23 s +/- 44 s for males. The mean duration of meals was 25 +/- 18 min for females and 11 +/- 8 min for males.
Subject(s)
Siphonaptera/physiology , Animals , Cats , Feeding Behavior , Female , Male , Time FactorsABSTRACT
A controlled clinical trial was undertaken to assess the efficacy of an application of a 0.07% deltamethrin shampoo against fleas and ticks in dogs. Twenty beagles, housed in separate cages, were randomly allocated into two groups of 10. The dogs were infested with 50 Ctenocephalides felis and 50 Rhipicephalus sanguineus each, and parasites were counted 24 h (fleas) and 72 h (ticks) later (Day 0). Dogs, when state, were then treated with 25 ml of a shampoo containing 0.07% deltamethrin (Group 1). Dogs in Group 2 were the controls. Fleas and ticks were counted 24 h (Day 1) and 48 h (Day 2) after the shampoo was applied, and the parasites were then removed. All the dogs were reinfested with fleas and ticks on Days 2, 7, 9, 14, 16 and 20. Parasites were counted 24 and 48 h after each reinfestation. Effectiveness against fleas was calculated 24 h after infestation, and against ticks 48 h after infestation. This study showed that the application of 25 ml of shampoo containing 0.07% deltamethrin to beagle dogs weighing between 10.2 and 12 kg was very well tolerated. It controlled the parasites present on the animals at the time of application: with an efficacy of 100% against fleas 24 h after treatment and an efficacy of 95% against ticks 48 h after treatment. The treatment protected against flea reinfestations with an efficacy of 100% during the first week, >98% in the second week and >95% in the third week. It also gave >99% protection against tick reinfestations in the first week and >96% in the second week.
Subject(s)
Dog Diseases/prevention & control , Ectoparasitic Infestations/veterinary , Insecticides/therapeutic use , Pyrethrins/therapeutic use , Siphonaptera/growth & development , Tick Infestations/prevention & control , Animals , Dog Diseases/parasitology , Dogs , Ectoparasitic Infestations/prevention & control , Female , Insecticides/administration & dosage , Male , Nitriles , Pyrethrins/administration & dosage , Ticks/growth & developmentABSTRACT
A field trial was undertaken to assess the efficacy of each of two formulations of ivermectin and of closantel in prevention and treatment of Oestrus ovis in a naturally infected flock grazing on the foothills of the Pyrenees mountains, in south-western France. Within the flock, 875 sheep were randomly divided into four groups, and treated twice during the fly season, with an interval of 60 days between treatments. Group 1 sheep were treated with albendazole (ABZ) at a dose rate of 3.8 mg/kg to maintain control of trichostrongylid parasites without affecting O. ovis; Group 2 received closantel at a dose rate of 10 mg/kg because of its known persistent activity against O. Ovis; Groups 3 and 4 received ivermectin at a dose rate of 200 mcg/kg bodyweight by subcutaneous injection (Isc) and orally (Io), respectively. All sheep were managed as a single group throughout the study. In order to assess the prophylactic effect of each product, immediately prior to the scheduled second treatment on Day 60 (D60), five sheep from each group were chosen at random and necropsied. Similarly, to assess the therapeutic effect, another five sheep from each group were selected on D70 and necropsied for parasite counts. During the 120 days of the trial, a significant number of animals from each group were regularly individually examined to assess their clinical status with regard to O. Ovis infection. Clinical signs of infection had significantly declined in Groups 2, 3 and 4 by 10 days after treatment reaching their lowest level at D30. In the control group during this period, clinical signs increased. Ten days after the second treatment, (D70), there was also evidence of a significant response to treatment. Finally the between-treatment differences in clinical scores of the closantel and ivermectin groups were small, although scores in Group 1 sheep was suggestive of a higher challenge in the second half of the study. On the basis of the postmortem counts and arithmetic means, prophylactic efficacies for the treatments relative to ABZ treated group, were 97.7, 62.5 and 0%, for the closantel, Isc and Io groups respectively. Therapeutic efficacies for the closantel, Isc and Io were 100, 100 and 98% respectively.
Subject(s)
Diptera , Insecticides/therapeutic use , Ivermectin/therapeutic use , Myiasis/veterinary , Salicylanilides/therapeutic use , Sheep Diseases , Administration, Oral , Albendazole/administration & dosage , Albendazole/therapeutic use , Animals , Female , Injections, Subcutaneous , Insecticides/administration & dosage , Ivermectin/administration & dosage , Male , Myiasis/drug therapy , Myiasis/prevention & control , Salicylanilides/administration & dosage , SheepABSTRACT
Jump performances of Ctenocephalides canis and Ctenocephalides felis felis have been measured and compared on unfed young imagos. The mean length of the C. felis felis jump was 19.9+/-9.1cm; minimum jump was 2cm, and the maximum was one 48cm. The C. canis jump was significantly longer (30.4+/-9.1cm; from 3 to 50cm). For height jump evaluation, grey plastic cylindric tubes measuring 9cm in diameter were used. Their height was increasing from 1 to 30cm by 1cm. Groups of 10 fleas of the same species were deposited on the base of the tube. The number of fleas which succeeded in jumping above the tube was recorded. The mean height jump carried out by 50% of fleas was calculated after linearisation of the curves: it was 15.5 and 13.2cm for C. canis and C. felis, respectively. The highest jump was 25 for C. canis and 17cm for C. felis.
Subject(s)
Cat Diseases/parasitology , Dog Diseases/parasitology , Ectoparasitic Infestations/veterinary , Siphonaptera/physiology , Animals , Cats , Dogs , MovementABSTRACT
Two experiments were conducted on dogs to evaluate interval to initiation and duration of the first blood meal of Ctenocephalides canis (Curtis). Percentage of fed male and female fleas was calculated for fleas held on dogs for 5, 15, 30, 60 min, 6, and 24 hr. Duration of first blood meal was also measured for individual fleas confined on dogs. When fleas were free in the hair coat, 21.2% had begun blood feeding within 5 min. After 1 hr, 72.5% of fleas had fed. After 6 hr, 95.2% of males and 100% of females had taken a blood meal, and 24 hr after deposition all fleas had fed. There was no significant difference between the 2 sexes. The mean delay between deposition and biting for fleas that began feeding within 15 min was 2 min 52 sec +/- 3 min 2 sec for female fleas and 3 min 8 sec +/- 2 min 45 sec for males. The mean duration of female and male meals was 5 min 3 sec +/- 3 min 41 sec and 6 min 9 sec +/- 6 min 8 sec, respectively. There was no significant difference between the 2 sexes. The dog flea took its blood meal on dogs more slowly than the cat flea did on cats; this meal was significantly longer for Ctenocephalides felis felis (Bouche) than for C. canis.
Subject(s)
Dog Diseases/parasitology , Ectoparasitic Infestations/veterinary , Siphonaptera/physiology , Animals , Blood , Cat Diseases/parasitology , Cats , Dogs , Ectoparasitic Infestations/parasitology , Feeding Behavior/physiology , Female , Host-Parasite Interactions , Male , Time FactorsABSTRACT
OBJECTIVE: To determine whether and for how long a single dose of a new injectable formulation of lufenuron would successfully control experimentally induced Ctenocephalides felis infestations in cats. ANIMALS: 15 cats (3 groups of 5). PROCEDURE: Each group of cats was housed in a separate room. Each cat was infested with 150 fleas, and flea counts were checked weekly. Once flea populations had stabilized, cats were treated with lufenuron at a dosage of 5 mg/kg of body weight, s.c. (group 1) or 10 mg/kg, s.c. (group 2). Group-3 cats were not treated. Flea counts were checked weekly for 18 weeks, and effectiveness was determined by comparing flea counts for treated cats with flea counts for control cats. Cats were reinfested with fleas during weeks 19, 26, 36, and 49, and flea counts were again determined weekly. RESULTS: Effectiveness for both dosages of lufenuron was > 90% by 5 weeks after treatment, > 95% by 9 weeks after treatment, and > 98% by 13 weeks after treatment. Reinfestations performed during weeks 19 and 26 were well controlled (ie, > 90% reduction in flea counts, compared with control group) in both groups of cats treated with lufenuron. Reinfestations performed during week 36 were well controlled only in cats treated with lufenuron at the higher dosage. Reinfestations performed during week 49 were not controlled in either group of treated cats. CONCLUSIONS: A single dose of the new injectable formulation of lufenuron should control flea populations in cats for up to 26 weeks, even among cats that are periodically reinfested with fleas.
Subject(s)
Benzamides/therapeutic use , Cat Diseases , Ectoparasitic Infestations/veterinary , Insecticides/therapeutic use , Siphonaptera , Animals , Benzamides/administration & dosage , Benzamides/adverse effects , Cats , Diptera , Ectoparasitic Infestations/drug therapy , Female , Injections, Subcutaneous , Insecticides/administration & dosage , Insecticides/adverse effects , Male , Time FactorsABSTRACT
OBJECTIVE: To compare effectiveness of deltamethrin- and diazinon-impregnated collars against fleas (Ctenocephalides felis) in an experimental infestation. ANIMALS: 30 Beagles. PROCEDURE: Dogs were housed in separate cages. Dogs were infested with 50 fleas each, and fleas were counted 24 hours later. Dogs were then fitted with a collar containing 4% deltamethrin (group 1) or 15% diazinon (group 2). Dogs in group 3 were controls. Fleas were counted 24 and 48 hours after collars were applied, and fleas were then removed. Dogs were reinfested with fleas on days 7, 14, 28, 60, 90, 120, 150, and 210. Fleas were counted 24 and 48 hours after each reinfestation. RESULTS: 48 hours after applying collars, flea counts were not notably decreased. Effectiveness in groups 1 and 2 was 24 and 29%, respectively. Infestation on day 7 was controlled, to a comparable extent, by both insecticides (effectiveness approx 85%). Effectiveness > 97% was obtained after collars had been worn for 14 days. Between days 14 and 91, both collars had comparable effectiveness (> 93%) that did not differ significantly. After day 91, effectiveness of the collar containing diazinon decreased sharply, whereas that of the collar containing deltamethrin remained > 90% until day 152. CONCLUSIONS AND CLINICAL RELEVANCE: The collar containing deltamethrin controlled flea populations on dogs beginning 14 days after application and continuing until day 150. The collar containing diazinon controlled infestation for 90 days. Deltamethrin-impregnated collars are a new formulation that can be introduced into a program to easily control fleas.
Subject(s)
Diazinon , Dog Diseases , Ectoparasitic Infestations/veterinary , Pyrethrins , Siphonaptera , Animals , Dogs , Ectoparasitic Infestations/prevention & control , Female , Insecticides , Male , NitrilesABSTRACT
OBJECTIVE: To determine the effect of an adulticide on flea populations of dogs and to evaluate efficacy of combined use of the adulticide and an insect growth regulator (IGR) in dogs with experimentally induced flea infestations. ANIMALS: 40 adult Beagles. PROCEDURE: Each group of 5 dogs was housed in a separate room. Each dog was infested 3 times with 50 fleas, and fleas were counted beginning on day -21. Groups of dogs and treatments (initiated on day 0) were as follows: 1, adulticide once; 2, adulticide on days 0 and 7; 3, adulticide on days 0, 3, and 7; 4, sham treatment; 5, IGR monthly; 6, IGR monthly plus adulticide once weekly for 6 weeks; 7, IGR monthly plus adulticide twice weekly for 6 weeks; 8, sham treatment. Flea counts were compared between treated and control dogs. RESULTS: By 24 hours after initial treatment, all adult fleas but 1 were dead in treated dogs. In groups 1 and 3, populations increased to 15 to 20 fleas/dog 2 months after treatment, compared with 48 fleas/dog in group 4. After treatment, mean flea counts were significantly lower for groups 1, 2, and 3, relative to group 4. Efficacy of treatment for group 5, relative to group 8, was > 94% after day 84. Efficacy of treatment for groups 6 and 7 was 99% after day 28. CONCLUSIONS AND CLINICAL RELEVANCE: Treatment with adulticide alone or in combination with an IGR had better efficacy, compared with sham treatment or IGR alone. Administration of adulticide twice weekly was not more efficacious than treatment once weekly.
Subject(s)
Dog Diseases/drug therapy , Ectoparasitic Infestations/veterinary , Insecticides/therapeutic use , Juvenile Hormones/therapeutic use , Siphonaptera , Administration, Oral , Animals , Dog Diseases/parasitology , Dogs , Drug Therapy, Combination , Ectoparasitic Infestations/drug therapy , Female , Insecticides/administration & dosage , Juvenile Hormones/administration & dosage , Random AllocationABSTRACT
Twenty-four beagles were randomly allocated into four groups of six and housed in separate cages. Each dog was infested with 25 Ctenocephalides canis and 25 Ctenocephalides felis felis and two days later (day 0) the dogs in groups 1, 2 and 3 received a spot-on application of selamectin (6 mg/kg), imidacloprid (10 mg/kg), or fipronil (6-7 mg/kg), respectively, while the dogs in group 4 were not treated. The dogs were combed 48 hours later, the fleas were removed, counted and their species were determined. All the dogs were reinfested with the same number of the two species of fleas on days 7, 14, 21, 28 and 35, and the efficacy of the treatments was calculated 48 hours after each infestation. The mean numbers of fleas on the control dogs were 19.8 C. canis and 14.7 C. felis felis. The three treatments were effective for the full 35 days of the trial; over the first 28 days, the efficacy of selamectin ranged from 81 to 100 and 92 to 99 per cent against C. felis felis and C canis, respectively, the efficacy of imidacloprid ranged from 98 to 100 per cent and the efficacy of fipronil was 100 per cent against both species. There were no significant differences between the three treatments.
Subject(s)
Dog Diseases/drug therapy , Ectoparasitic Infestations/veterinary , Imidazoles/therapeutic use , Insecticides/therapeutic use , Ivermectin/analogs & derivatives , Ivermectin/therapeutic use , Pyrazoles/therapeutic use , Siphonaptera , Administration, Cutaneous , Animals , Dogs , Ectoparasitic Infestations/drug therapy , Female , Imidazoles/administration & dosage , Insecticides/agonists , Ivermectin/administration & dosage , Male , Neonicotinoids , Nitro Compounds , Pyrazoles/administration & dosage , Treatment OutcomeABSTRACT
The amounts of active ingredient required to kill adult Ctenocephalides felis fleas on filter insecticide impregnated papers were determined in an attempt to compare the activity of different active ingredients. The following compounds were tested: bioallethrin, deltamethrin, esbiothrin and permethrin. The LD50 and LD90 against Ctenocephalides felis were 121 and 770 mg/m2 respectively for bioallethrin, and 161 and 671 mg/m2 respectively for esbiothrin. For deltamethrin and permethrin, the LD50 were 0.38 and 23 mg/m2 respectively, and 15 and 60 mg/m2 respectively for LD90.
Subject(s)
Insecticides , Pyrethrins , Siphonaptera , Animals , Cats , Drug Evaluation , Lethal Dose 50ABSTRACT
Evaluation of insecticidal activity of flea products is generally based on counting live fleas in the animal's coat 24 and 48 hours following artificial infestation. This approach, however, does not allow to specify whether the fleas have had the opportunity to bite and take a bloodmeal prior to their death. To address this question, 30 cats were alloted to six groups of five animals. Each cat was housed in a separate cage. At Day 0, each group of cats received a single treatment as follows: Group 1: spot-on application of imidacloprid: cats < 4 kg: 40 mg/cat, cats > or = 4 kg: 80 mg/cat (Advantage). Group 2: spot-on application of fipronil: 50 mg/cat (Frontline spot-on). Group 3: spray application of fipronil: 7.5 mg/kg b.w. (Frontline spray). Group 4: foam application of permethrin 40/60: 50 mg/kg b.w. (Defencat). Group 5: aerosol spray application of dichlorvos + fenitrothion: one second/kg b.w. (NuvanTop). Group 6: control group: cats were left untreated. One hour after treatment, each cat was infested with 50 unengorged young adult fleas, Ctenocephalides felis, deposited along the dorsal midline. One hour later, each cat was carefully combed using a fine-toothed comb (13 teeth/cm). Collected fleas were swatted to deteci blood in their abdomen. To the manufacturers respective product use instructions and efficacy claims, reeinfestations were made at Days 3, 7, 14 in all groups; at Days 21 and 30 in Groups 1, 2, 4, 6; at Days 35 and 42 in Groups 3 and 6. The cats were combed one hour after each reinfestation. The results indicate that the topical application of imidacloprid or fipronil does not prevent fleas from biting and feeding within the first hour after infestation prior to being killed while the topical application of dichlorvos/fenitrothion and permethrin let to a better than 80% decrease of the number of engorged fleas for three and seven days post treatment, respectively.
Subject(s)
Cat Diseases/prevention & control , Ectoparasitic Infestations/veterinary , Insecticides/pharmacology , Siphonaptera/drug effects , Administration, Topical , Aerosols , Animals , Cat Diseases/parasitology , Cats , Dichlorvos/administration & dosage , Dichlorvos/pharmacology , Dichlorvos/therapeutic use , Ectoparasitic Infestations/parasitology , Ectoparasitic Infestations/prevention & control , Feeding Behavior/drug effects , Female , Fenitrothion/administration & dosage , Fenitrothion/pharmacology , Fenitrothion/therapeutic use , Imidazoles/administration & dosage , Imidazoles/pharmacology , Imidazoles/therapeutic use , Insecticides/administration & dosage , Insecticides/therapeutic use , Male , Neonicotinoids , Nitro Compounds , Permethrin , Pyrazoles/administration & dosage , Pyrazoles/pharmacology , Pyrazoles/therapeutic use , Pyrethrins/administration & dosage , Pyrethrins/pharmacology , Pyrethrins/therapeutic use , Siphonaptera/physiology , Time FactorsABSTRACT
Giardiasis is an intestinal parasitosis affecting dogs and able to infect human beings. Its diagnosis can not be done with the only clinical signs, the main of which is non characteristic diarrhoea. It implicates to perform further tests to detect the parasite. The zinc sulfate concentration technique (ZSCT) is the more effective one if performed on two or three successive days. Fecal ELISA kits have been developed to detect Giardia in humans and were found to be less sensitive than the ZSCT in dogs. In this study, we used 30 infected Beagles to compare the sensitivity of one, two or three fecal examinations following ZSCT and one or two ELISA tests. We conclude that if a single ZSCT is insufficient, two or three ZSCT and one or two ELISA using the commercial kit ProSpecT Giardia have almost the same sensitivity.
Subject(s)
Dog Diseases/diagnosis , Giardiasis/veterinary , Animals , Antigens, Protozoan/analysis , Diarrhea/diagnosis , Diarrhea/parasitology , Diarrhea/veterinary , Dog Diseases/parasitology , Dogs , Enzyme-Linked Immunosorbent Assay/methods , Enzyme-Linked Immunosorbent Assay/veterinary , Feces/parasitology , Female , Giardia/immunology , Giardia/isolation & purification , Giardiasis/diagnosis , Giardiasis/parasitology , Male , Sensitivity and Specificity , Zinc SulfateABSTRACT
Some pharmacokinetic parameters of selamectin were determined in male (n = 5) and female (n = 5) Beagle dogs following a topical application at a dose rate of 6 mg/kg. The plasma concentration versus time data for the drug were analysed using a one-compartment model. The maximum plasma concentrations of 12.72 +/- 5.13 ng/ml for males and 22.65 +/- 11.95 ng/ml for females occurred around 5 days after administration. The area under the concentration-time curve (AUC) was 192.08 +/- 63.85 ng.day/ml for males and 370.97 +/- 146.87 ng.day/ml for females. The mean residence time was the same in males and females (12.55 days). This study reveals a sex-influence on the disposition of selamectin in the plasma of dogs, which implies that further information will be needed for correlation with efficacy studies in dogs.
Subject(s)
Dogs/blood , Ivermectin/analogs & derivatives , Ivermectin/pharmacokinetics , Administration, Topical , Animals , Antiparasitic Agents/administration & dosage , Antiparasitic Agents/blood , Antiparasitic Agents/pharmacokinetics , Female , Half-Life , Ivermectin/administration & dosage , Ivermectin/blood , Male , Metabolic Clearance RateABSTRACT
INTRODUCTION: Cutaneous dirofilariasis caused by Dirofilaria repens is a parasitic disease in dogs and cats. It is exceedingly rare in man who is an occasional host. The parasite fails to reach maturity. The true incidence of dirofilariasis is probably greater than recognized. This can be explained by the apparent benign nature of the lesions that may not warrant excision. CASE REPORT: A 12-year-old girl presented a cutaneous nodular lesion involving the forehead. Surgical excision allowed diagnosis: cutaneous dirofilariasis caused by Dirofilaria repens. The child had always stayed in mainland France. Histopathological examination showed the worm trial with inflammatory granulomatous reaction of the deep dermis. The parasite extraction allowed successful treatment. DISCUSSION: This new case of cutaneous dirofilariasis emphasizes that this parasitic disease is present in mainland France. The infection is more common in Southern France. The present report describes a pediatric case in Gironde, where it is exceedingly rare. Microscopic examination identified, and it was unusual, a male worm.